• BMB Reports
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• 제43권9호
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• pp.609-613
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• 2010

The Dvl and Axin proteins, which are involved in the Wnt signaling pathway, each contain a conserved DIX domain in their sequences. The DIX domain mediates interaction between Dvl and Axin, which together play an important role in signal transduction. However, the extremely low production of DIX domain fragments in E. coli has prevented more widespread functional and structural studies. In this study, we demonstrate that the DIX domains of Dvl and Axin are expressed noticeably in a multi-cistronic system but not in a mono-cistronic system. Formation of the $DIX_{Dvl1}-DIX_{Axin1}$ complex was investigated by affinity chromatography, SEC and crystallization studies. Unstable DIX domains were stabilized by complexing with counterpart DIX domains. The results of the preliminary crystallization and diffraction of the $DIX_{Dvl1}-DIX_{Axin1}$ complex may prove useful for further crystallographic studies.

• BMB Reports
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• 제38권2호
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• pp.243-247
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• 2005

Dishevelled (Dvl) is a positive regulator of the canonical Wnt signaling pathway, which regulates the levels of $\beta$-catenin. The $\beta$-catenin oncoprotein depends upon the association of Dvl and Axin proteins through their DIX domains, and its accumulation directs the expression of specific developmental-related genes at the nucleus. Here, the $^1H$, $^{13}C$, and $^{15}N$ resonances of the human Dishevelled 2 DIX domain are assigned using heteronuclear nuclear magnetic resonance (NMR) spectroscopy. In addition, helical and extended elements are identified based on the NMR data. The results establish a structural context for characterizing the actin and phospholipid interactions and binding sites of this novel domain, and provide insights into its role in protein localization to stress fibers and cytoplasmic vesicles during Wnt signaling.

• 지구물리와물리탐사
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• 제22권4호
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• pp.225-238
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• 2019

최적의 심도 영역 속도를 도출하기 위한 구조보정 속도분석(MVA, migration velocity analysis) 기법을 해양에서 취득한 원거리 다중채널 탄성파 자료에 적용하여 그 효용성을 확인한다. 지금까지 통상적으로 수행된 시간 영역 자료처리 결과는 지질학적 층서해석에는 무리 없는 결과이나, 어느 정도 가능성이 있는 플레이나 리드 지역에서의 유가스 탐사에서는 저류층 지질모델 구축, 시추 설계, 매장량 계산에서 반드시 심도 영역 속도 구조와 영상이 필요하다. 데이터 영역에서 근사 방식을 사용한 공통 중간점 기반 속도 분석으로부터 도출한 속도는 처음부터 오차를 내재하여 불확실성이 높다. 반면에, 이를 보완해 줄 검층 자료가 없는 상황에서 실측 규모의 속도 구조를 도출하는데 있어 이미지 영역 구조보정 속도분석 기법은 상당히 효율적인 방법이다. 이 연구에서는 해양에서 취득한 다중 채널 탄성파자료에 대해 합리적인 결과를 도출하기 위해 시간 영역에서 신호의 품질을 최적화하고, 이 자료에 대하여 반복적으로 MVA 기법을 적용함으로써 심도영역 속도 및 구조보정 단면도를 도출하였다. 시간 영역 속도를 단순히 Dix 방정식에 의해 심도영역으로 변환한 속도를 이용하여 생성한 결과(공통 수신점 모음도 및 중합 단면도)와 MVA 기법을 이용한 심도영역 자료처리를 통해 도출된 속도를 이용하여 생성한 결과를 비교함으로써, 심도영역 결과가 보다 합리적임을 확인하였다. 심도 영역으로 도출된 속도는 중합전 심도 구조보정에 바로 사용될 수 있을 뿐만 아니라, 현장 자료의 파형역산 적용 시 초기 모델로 활용함으로써 역산 수행 과정에서 발생할 수 있는 국부 최소(local minima) 문제를 최소화할 수 있다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.638-642
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• 2005

For gas hydrate exploration, long offset multichannel seismic data acquired using by the 4km streamer length in Ulleung basin of the East Sea. The dataset was processed to define the BSRs (Bottom Simulating Reflectors) and to estimate the amount of gas hydrates. Confirmation of the presence of Bottom Simulating reflectors (BSR) and investigation of its physical properties from seismic section are important for gas hydrate detection. Specially, faster interval velocity overlying slower interval velocity indicates the likely presences of gas hydrate above BSR and free gas underneath BSR. In consequence, estimation of correct interval velocities and analysis of their spatial variations are critical processes for gas hydrate detection using seismic reflection data. Using Dix's equation, Root Mean Square (RMS) velocities can be converted into interval velocities. However, it is not a proper way to investigate interval velocities above and below BSR considering the fact that RMS velocities have poor resolution and correctness and the assumption that interval velocities increase along the depth. Therefore, we incorporated Migration Velocity Analysis (MVA) software produced by Landmark CO. to estimate correct interval velocities in detail. MVA is a process to yield velocities of sediments between layers using Common Mid Point (CMP) gathered seismic data. The CMP gathered data for MVA should be produced after basic processing steps to enhance the signal to noise ratio of the first reflections. Prestack depth migrated section is produced using interval velocities and interval velocities are key parameters governing qualities of prestack depth migration section. Correctness of interval velocities can be examined by the presence of Residual Move Out (RMO) on CMP gathered data. If there is no RMO, peaks of primary reflection events are flat in horizontal direction for all offsets of Common Reflection Point (CRP) gathers and it proves that prestack depth migration is done with correct velocity field. Used method in this study, Tomographic inversion needs two initial input data. One is the dataset obtained from the results of preprocessing by removing multiples and noise and stacked partially. The other is the depth domain velocity model build by smoothing and editing the interval velocity converted from RMS velocity. After the three times iteration of tomography inversion, Optimum interval velocity field can be fixed. The conclusion of this study as follow, the final Interval velocity around the BSR decreased to 1400 m/s from 2500 m/s abruptly. BSR is showed about 200m depth under the seabottom