• The Korean Journal of Pharmacology
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• v.32 no.1
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• pp.113-123
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• 1996

It has been well known that polyamines ensure the stability of chromatin structure and the fidelity of DNA transcription. This study was carried out to evaluate the effect of polyamines on the apoptosis of mouse thymocytes induced by dexamethasone and polyamine synthesis inhibitors. 1) In the histological death findings of thymocytes double-stained with acridine orange and ethidium bromide, the apoptotic and the necrotic fractions (AF; NF) in the control group were $9.4{\pm}4.2%$ and $4.5{\pm}5.3%$, respectively. Dexamethasone $(3\;{\times}\;10^{-8}\;M:\;DX)$ in creased AF upto $52.0{\pm}8.1%$ and did not change NF, but A23187 $(5\;{\times}\;10^{-7}\;M:\;A2)$ increased AF and NF upto $45.0{\pm}8.9%$ and $20.5{\pm}10.6%$, respectively. 2) The thymocyte viability was significantly reduced by DX, DHEA $(1\;{\times}\;10^{-4}\;M)$, A2, DFMO $(1\;{\times}\;10^{-4}\;M)$, and $MGBG\;(1\;{\times}\;10^{-4}\;M)$, respectively. It was, however, little affected by $aminoguanidine\;(1\;{\times}\;10^{-4}\;M:\;AG)$, $putrescine\;(1\;{\times}\;10^{-5}\;M:\;PT)$, $spermidine\;(1\;{\times}\;10^{-5}\;M:\;SD)$, and $spermine\;(1\;{\times}\;10^{-5}\;M:\;SM)$. 3) The genomic DNA of mouse thymocyte was markedly fragmented by DX and A2, respectively, and to a lesser extent, by DHEA, but was little affected by MGBG, DFMO, AG, and each of polyamines. 4) The DX induced reduction of thymocyte viability was moderately attenuated by DHEA, but little affected by DFMO, MGBC, and AG. However, SM significantly attenuated the viability reduction induced by A2 as well as DX. 5) The thymocyte viability reduction by MGBG and DFMO was significantly attenuated by only SM among three polyamines applied in this study. 6) The thymocyte viability redution by combined treatments of DX with DFMO and MGBG, respectively, was significantly attenuated by SM, and moderately by PT. But the viability reduction by combined treatment of DX with AG or DHEA was not affected by polyamines. These results suggest that polyamines, particularly spermine, might play the inhibitory role in thymocyte apoptosis and the inhibitory effect can be ascribed in part to the increase of polyamine uptake by thymocytes pretreated with DFMO and MGBG.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.10b
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• pp.118-118
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• 2003

The influence of dietary supplementation with dehydroepiandrosterone-sulphate (DHEA-S) at 0.6% was investigated in male Syrian golden hamsters initiated by treatments with azoxymethane(AOM), and dihydroxy-di-n-propyl nitrosamine (DHPN), timed after transfer from a choline-deficient to a normal diet.(omitted)

• Journal of the Korea Convergence Society
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• v.11 no.8
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• pp.323-330
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• 2020

A purpose of this study is to investigate the effects of 12-Weeks regular physical activities on female senior adults aging related hormone(HGH, DHEA-S, estrogen) levels and a risk factor in cardiovascular disease(adiponectin, hs-CRP). Twenty female senior adults participated in this study and divided intoexercise group(n=10) and control group(n=10). The Korean traditional dance program was conducted three times a week(60min) for 12-weeks. Paired t-test was used to examone the differences within experimental groups and independent t-test was used to changes the differences between groups. The followings are the results of this study. Estrogen(p=0.025), HGH(p=0.009), DHEA-S(p=0.009), adiponectin(p=0.014) was significantly increased after 12-weeks program for exercise group, And hs-CRP(p=0.010) was significantly decreased for exercise group. Estrogen(p=0.006), DHEA-S(p<.0.002), adiponectin(p=0.008), hs-CRP(p=0.004) was significantly changed between exercise group and control group.

• The Korean Journal of Pharmacology
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• v.27 no.1
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• pp.81-88
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• 1991

The bilateral castration of male mice was operated under light ether anesthesia, and the sham operated mice were considered as the uncastrated. The treatments of mice with the following steroids were started one hour after operation. Hydrocortisone 50 mg/kg (HC), dehydroepiandrosterone 250 mg/kg (DHEA), ${\beta}-estradiol$ 5 mg/kg (E2), and testosterone 20mg/kg (TS) were subcutaneously injected into male ICR mice at noon for four days. Animals were sacrificed in the next-morning (at 10-12 A.M.) after the last injection. The intestinal putrescine(PT) content was lower and the liver and intestinal spermine(SM) contents were higher in castrated mice(CM), comparing with those of uncastrated mice (UCM). The intestinal PT content of UCM was markedly increased HC. But all brain polyamines of CM were significantly decreased by it. And HC also increased the spermidine(SD) content of kidney and liver and the intestinal PT content in CM. E2 induced the marked increase of liver PT content with the moderate increase of renal SD in UCM. And E2 significantly increased the brain and liver PT contents and the all renal polyamine contents in CM. Both of DHEA and TS induced the increase of renal PT content in UCM, and they also induced the marked increases of all renal polyamines of CM. In addition, TS increased the brain SM of CM. These results suggest that the steroidal regulation mechanism of brain, kidney, liver, and intestine seems to be different from one another, and the renal activity of polyamine synthesis can be markedly enhanced by sex steroids.

• 건강소식
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• v.25 no.12 s.277
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• pp.18-19
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• 2001

• Journal of Pharmaceutical Investigation
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• v.41 no.1
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• pp.37-43
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• 2011

Urinary steroid levels were investigated in the treatment of CKD-501, a new anti-diabetic drug candidate. CKD-501 was administered orally at the dosage of 1, 2, 4 mg/day for 7 days to normal men (n=18). Urine was collected before, during and after stopping the drug administration and the urinary level of androgen, estrogen, progestin and corticoids were quantified using GC-MS (gas chromatography-mass spectrometry). Only urinary corticosteroid and an androgen, DHEA levels among all the analyzed steroids, have been found to increase progressively, reaching significant levels on the last day of drug treatment and later declined after the drug treatment is withdrawn. Therefore, it was thought that an increase in the urinary corticoid and DHEA levels could be a characteristic of CKD-501, since it prominently acts on the glucose sensitivity and suppresses the triglyceride levels. In conclusion, it was found that CKD-501, an anti-diabetic drug candidate, affects the glucocorticoid and DHEA levels and it plays a crucial role in glucose homeostasis.

• Perspectives in Nursing Science
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• v.2 no.1
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• pp.19-36
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• 2005

Muscle atrophy is defined as a decrease in muscle mass, cross-sectional area, and myofibrillar protein content. Causes inducing muscle atrophy may be inactivity, denervation, undernutrition and steroid. Inactivity may decrease protein synthesis and increase protein breakdown of skeletal muscle. The muscle atrophy due to inactivity was induced by bed rest, hindlimb suspension, cast, total hip replacement arthroplasty, anterior cruciate ligament reconstruction. Denervated atrophy may be induced by the loss of innervation from lower motor neuron. The atrophy was apparent in the lower limb of hemiplegic patients following ischemic stroke and in the hindlimb of ischemic stroke rats. Protein breakdown of skeletal muscle in the undernourished state results in muscle atrophy. The atrophy due to undernutrition was evident in cancer and leukemia patients and in the undernourished rats. Steroids have been used to treat allergies, inflammatory diseases, autoimmune diseases and to inhibit immune function following transplantation. Steroids may induce muscle atrophy by protein breakdown of skeletal muscle. Muscle Physiology Laboratoryat College of Nursing, Seoul National University proved that dexamethasone may induce hindlimb muscle atrophy in rats and exercise and DHEA may attenuate hindlimb muscle atrophy induced by the steroid in rats. Nurses working with patients undergoing steroid treatment need to be cognizant of steroid induced muscle atrophy. They need to assess whether muscle atrophy is being occurred during and after the steroid treatment. Moreover, they need to apply exercise and DHEA to the patients undergoing steroid treatment in order to attenuate the steroid induced muscle atrophy.

• Journal of the Korean Society of Physical Medicine
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• v.10 no.1
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• pp.53-61
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• 2015

PURPOSE: This study examined the effect of the combined exercise program on physical fitness and related hormone in elderly women. METHODS: The subjects included 40 elderly women who reside at B city. Upon thire agreements, the subjects were divided into either an experimental group and the control group. there 20 subjects in each. The combined exercise program was conducted during the 8 weeks, and the experimental group was underwent its associated program 5 times a week. there wear 2 subjects from each group that were excluded. The physical fitness wear measured and the related hormone(growth hormone, DHEA) were taken with blood serum density. RESULTS: After 8 weeks for intervention, there were statistically significant differences between in physical fitness and growth hormone, DHEA in experimental group(p<.05). however, this difference was not significantly different in the control group. Futher, there wear statistically significant difference between two group of all item(p<.05). CONCLUSION: The findings of this study show that combined exercise program helps the physical fitness and increase of growth hormone, DHEA in elderly women. In conclusion, the regular combined exercise program for 8 weeks is effective for increase physical fitness and related hormone in elderly women, and positive influence upon, thereby being thought to be able to lower risk in aging and weakness.

• Biomolecules & Therapeutics
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• v.25 no.3
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• pp.321-328
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• 2017

Steroid sulfatase (STS) is an enzyme responsible for the hydrolysis of aryl and alkyl sulfates. STS plays a pivotal role in the regulation of estrogens and androgens that promote the growth of hormone-dependent tumors, such as those of breast or prostate cancer. However, the molecular function of STS in tumor growth is still not clear. To elucidate the role of STS in cancer cell proliferation, we investigated whether STS is able to regulate the integrin signaling pathway. We found that overexpression of STS in HeLa cells increases the protein and mRNA levels of integrin ${\beta}1$ and fibronectin, a ligand of integrin ${\alpha}5{\beta}1$. Dehydroepiandrosterone (DHEA), one of the main metabolites of STS, also increases mRNA and protein expression of integrin ${\beta}1$ and fibronectin. Further, STS expression and DHEA treatment enhanced phosphorylation of focal adhesion kinase (FAK) at the Tyr 925 residue. Moreover, increased phosphorylation of ERK at Thr 202 and Tyr 204 residues by STS indicates that STS activates the MAPK/ERK pathway. In conclusion, these results suggest that STS expression and DHEA treatment may enhance MAPK/ERK signaling through up-regulation of integrin ${\beta}1$ and activation of FAK.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.2
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• pp.141-151
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• 1998

Objectives: To determine whether the body weight, body mass index (BMI), and basal serum level of LH, FSH, testosterone (T), dehydroepiandrosterone sulfate (DHEA-S) are related to the ovarian response to clomiphene citrate (CC) in patients with polycystic ovarian syndrome (PCOS). Materials and Method: From January 1996 to June 1997, total 57 patients with PCOS were enrolled in the present study. Women who had other infertility factors were excluded from our study. The ovulation induction using CC was used in all patients. The patients were grouped into 50 mg group, 100 mg group, and 150 mg group according to their daily CC dose. The patients were also grouped to ovulatory and non-ovulatory group. The body weight, BMI, and basal serum level of LH, FSH, T, DHEA-S were measured in all patients on the 2nd or 3rd day of the menstrual cycle. Results were analysed with Student's t-test and Fisher's exact test. Results: The body weight and BMI of the nonovulating group were significantly higher than those of the ovulating group in all groups (50, 100, 150 mg of CC). However, there were no significant differences of the level of LH and FSH between ovulating and nonovulating groups in all CC groups (50, 100, 150 mg). The level of T of nonovulating group was significantly higher in 50 and 100 mg of CC groups, but not in 150 mg group. The level of DHEA-S of the non-ovulating group is significantly higher in 50 mg group, but not in 100 and 150 mg groups. Conclusion: The body weight and BMI could be useful predictors of ovarian response to CC in patients with PCOS, and basal T and DHEA-S also might be useful in cases of low-dose CC treatment.