• 천문학회보
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• 제44권1호
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• pp.82.2-82.3
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• 2019

Korea Astronomy and Space Science Institute (KASI), in collaboration with the NASA Goddard Sparce Flight Center (GSFC), will develop a next generation coronagraph for the International Space Station (ISS). COronal Diagnostic EXperiment (CODEX) uses multiple filters to obtain simultaneous measurements of electron density, temperature, and velocity within a single instrument. CODEX's regular, systematic, comprehensive dataset will test theories of solar wind acceleration and source, as well as serve to validate and enable improvement of space-weather/operational models in the crucial source region of the solar wind. CODEX subsystems include the coronagraph, pointing system, command and data handling (C&DH) electronics, and power distribution unit. CODEX is integrated onto a standard interface which provides power and communication. All full resolution images are telemeters to the ground, where data from multiple images and sequences are co-added, spatially binned, and ratioed as needed for analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.393-399
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• 2004

Useful genes can be Screened from various environments by construction of metagenomic DNA libraries. In this study, water samples were collected from several lakes in mid Korea, and analyzed by T-RFLP to examine diversities of the microbial communities. The crude DNAs r were extracted by the SDS-based freezing-thawing method, and then further purified using an $UltraClean^{TM}$ kit (MoBio, USA). The metagenomic libraries were constructed with the DNAs partially digested with EcoR I, BamH I, and Sac II in Escherichia coli DH 10B using the pBACe3.6 vector. About 44.0 Mb of metagenomic libraries were obtained with average inserts 13-15 kb in size. The bphC genes responsible for degradation of aromatic hydrocarbons via mets-cleavage were identified from the metagenomic libraries by colony hybridization using the bphC specific sequence as a probe. The 2,3-dihydroxybiphenyl (2, 3-DHBP) dioxygenase gene (bphC ), capable of degradation of 2,3-DHBP, was cloned and its nucleotide Sequences analyzed. The genes consisted of 966 and 897 base pairs with an ATG initiation codon and a TGA termination codon. The activity of the 2,3-DHBP dioxygenase was highly expressed to 2,3-DHBP and Showed a broad substrate range to 2,3-DHBP, catechol, 3-methylcatechol and 4-methylcatechol. These results in-dicated that the bphC gene identified from the metagenomes derived from lake water might be useful in the development of a potent strain for degradation of aromatic pollutants.

• 생명과학회지
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• 제9권3호
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• pp.253-261
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• 1999

Leptin gene, an obesity gene, has been known to involve in the regulation of food intake and body weight. It is also thought to be related to the glucose metabolism, insulin secretion and type II diabetes mellitus. Recently, the production of recombinant leptin protein has been attempted for the application in the treatment of obesity and the correction of hereditary obesity and type II diabetes. In the present study, leptin cDNA was cloned from mouse fat cells by RT-PCR and prokaryotic expression of leptin was attempted in order ot prepare a leptin-specific antigen. Immunization of a rabbit with the leptin-specific antigen into a rabbit resulted in the generation of leptin-specific antiserum that could be useful in the detection of leption expressed in various tissues. The sequence of leptin cDNA prepared in the present study wa identical to the previously reported one. Transformation of E. coli(DH5a) cells with the leptin cDNA-inserted translation vector, pGEX-4T-3-leptin followed by treatment with IPTG (0.1mM) resulted in the expression of a large amount of GST-leptin fusion protein with a molecular weight of 44 KDa as an inclusion body. Denaturation of the insoluble fusion protein by 8M urea, 6M guanidium-HCI or 0.1% 2-mercaptoethanol followed by a slow oxidation could not solubilize the inclusion body. The cell extract was subjected to SDS-PAGE and GST-leptin protein electroeluted from the gel was then injected into a rabbit subcutaneously for the immunization. Anti-GST-leptin rabbit antiserum which had a cross reactivity to the GST-leptin protein was generated. Leptin protein expressed in mouse brain and fat tissues was detected by Western blot immunodetection system using the antiserum generated in the present study.

• 천문학회보
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• 제44권2호
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• pp.57.4-57.4
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• 2019

BITSE is a project of balloon-borne experiments for a next-generation solar coronagraph developed by a collaboration with KASI and NASA. The coronagraph is built to observe the linearly polarized brightness of solar corona with a polarization camera, a filter wheel, and an aperture door. For the observation, the coronagraph is supported by the power distribution unit (PDU), a pointing system WASP (Wallops Arc-Second Pointer), telemetry & telecommand system SIP (Support Instrument Package) which are developed at NASA's Goddard Space Flight Center, Wallops Flight Facility, and Columbia Scientific Balloon Facility. The BITSE Command and Data Handling (C&DH) system used a cost-off-the-shelf electronics to process all data sent and received by the coronagraph, including the support system operation by RS232/422, USB3, Ethernet, and digital and analog signals. The flight software is developed using the core Flight System (cFS) which is a reusable software framework and set of reusable software applications which take advantage of a rich heritage of successful space mission of NASA. The flight software can process encoding and decoding data, control the subsystems, and provide observation autonomy. We developed a python-based testing framework to improve software reliability. The flight software development is one of the crucial contributions of KASI and an important milestone for the next project which is developing a solar coronagraph to be installed at International Space Station.

• 치위생과학회지
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• 제18권6호
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• pp.340-348
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• 2018

본 연구는 치과위생사 의료인화에 대한 요구와 논의가 있는 현 시대적 상황에서 치과의료인력을 대상으로 치과위생사를 위한 선행과제와 의료인이 되었을 때의 긍정적 부정적 효과, 확장 가능한 전문업무에 대해 파악하고자 하였다. 치과위생사 259명, 치과의사 128명을 대상으로 치과위생사 의료인화에 대한 인식과 선행 과제, 긍정 및 부정적 효과와 확장 가능한 전문업무 등에 대해 조사 분석하여 다음과 같은 결론을 얻었다. 치과위생사 의료인화에 대해 치과위생사의 94.2%, 치과의사의 46.9%가 인지하였고, 치과위생사는 협회매체(52.5%), 치과의사는 치과신문(23.4%)을 통해 인지하는 경우가 많았다. 찬성도는 치과위생사의 95.0%, 치과의사의 64.1%였으며, 당위성은 치과위생사가 84.9%, 치과의사 51.6%였다. 당위성의 근거로 치과위생사는 업무 전문성과 교육과정 부합성이 높았고 업무 부합성이 낮았으나 치과의사는 의료체계 유사성이 높았고 교육과정 부합성이 가장 낮았다. 의료인화를 위한 선행 과제 중 직업적 영역에서 치과위생사와 치과의사 모두 직업의식과 윤리의식이 높았다. 제도적 영역에서도 두 직종 모두 치위생교육 질 관리가 가장 높았으며, 학제 일원화가 가장 낮았다. 사회적 영역에서 치과위생사는 중앙정부설득과 국민공감대 형성이 높았고, 치과의사는 유관단체 협력과 중앙정부 설득이 높았다. 치과위생사 의료인화 시 긍정적 효과는 치과위생사 인식 확장이 치과위생사와 치과의사 모두 가장 높았고, 부정적 효과는 치과위생사의 경우 임금상승 심화와 위임진료 증가가 높았고, 치과의사는 임금상승 심화와 구인난 심화가 높았다. 의료인화 시 확장 가능한 전문업무로는 독자적 치주관리프로그램 운영으로 치과위생사(79.9%)와 치과의사(69.6%) 모두에게 가장 높게 나타났다. 치과위생사는 치주관리를 위한 진단(44.4%)과 전신질환자 구강건강관리(44.0%), 비외과적 치주처치(41.3%), 근육 정맥주사(27.4%) 순이었고, 치과의사는 근육 정맥주사(44.1%), 비외과적 치주처치(34.3%), 전신질환자 구강건강관리(29.4%), 치주관리를 위한 진단(13.7%) 순이었다. 이상의 결과를 통해 치과위생사 의료인화가 가지는 의미에 대한 충분한 검토와 논의가 필요할 것으로 생각되고, 의료인화와 관련하여 치과위생사의 업무가 합리적으로 조정되고 합법화하는 데 본 연구가 활용되기를 기대한다.

• 한국지반공학회논문집
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• 제15권6호
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• pp.29-44
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• 1999

본 논문은 균질 및 비균질의 사질토 지반에서 항타 시공된 단일 강성말뚝의 수평거동에 대한 모형실험 결과들을 고찰하였다. 본 연구의 목적은 말뚝의 수평거동 특성에 대한 말뚝 시공상태 (Driven & Embedded), 말뚝 근입길이에 대한 하부지반의 두께비 (H/L),그리고 지반의 상대밀도의 영향에 관하여 실험적인 연구를 수행하고 이러한 영향들을 정량화 할 수 있는 실험결과를 얻었다. 모형 실험 결과들에 의하면, 수평거동은 느슨한 균질지반에서 시공방법에 상당히 의존하는 것으로 나타났다. 말뚝시공시 항타방법에 의존할 경우 균질지반에서는 느슨한 지반일수록 그리고 비균질 지반 $(E_{h1}/E_{h2}/=0.18)$에서는 상부층 두께가 클수록 수평변위 감소에 상당한 효과를 얻을 수 있으나, 최대 휨모멘트 감소는 수평변위 감소와 정반대의 결과를 나타냈다. 수평변위 측면에서, 매입말뚝에 대한 항타말뚝의 변위비 $(y_{Driven}y_{Embedded})$는 균질지반의 각 상대 밀도에 대하여 0.65-0.88 $(D_r=90%)$와 0.38-0.65 (D$_{r}$=61.8%)의 범위로 그리고 비균질지반에서 0.6-0.88 의 범위로 나타났다. 또한, 본 연구에서는 $y_D/y_E \;와\; NBM_D/MBM_E$에 대한 항타고와 H/L의 영향들을 모형실험 결과들로부터 실험식으로 제안하였다.

• 대한의생명과학회지
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• 제2권2호
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• pp.175-185
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• 1996

모든 진핵세포에 존재하며 세포의 성장 및 분화에 주로 관계되는 신호전달물질의 하나인 Mitogen-activated protein(MAP)kinase의 mitogen에 의한 핵내 활성화와 기질 인산화에 대해 알아보기 위해 본 실험을 수행하였다. P388세포를 10% fetal bovine serum이 첨가된 DMEM배지에 배양한 후, 혈청이 들어있지 않은 배지에서 24시간 더 배양하고 serum 및 PMA를 농도별로 처리하여 세포성 장을 위한 최적 농도를 확인한 결과 serum은 5-20% 농도에서 세포성장을 촉진시켰고 PMA는 실험한 모든 농도에서 세포성장을 거의 촉진시키지 못하는 경향을 확인하였다. 이어 P388 세포를 serum 및 PMA로 10 분간 활성화하여 파쇄한후 세포질분획과 핵분획으로 분리하여 각 분획을 10% gel 상에서 전기영동 하여 nitrocellulose paper에 옳긴 후 anti-ERKI antibody를 이용해 확인해본 결과 serum, PMA로 처리된 세포 모두에서 MAP kinase의 핵내 이동이 관찰되었으며 특히 세포질 내에 주로 존재하는 42, 44 Kd의 MAP kinase isoform중 42 Kd의 isoform이 주로 핵내로 이동되는 것이 관찰되었다. MAP kinase의 기질인산화 실험을 위해 serum으로 활성화시킨 세포를 파쇄하여 SP-sephadex C-50, Phenyl superose, Mono Q column의 순서로 chromatography를 시 행하여 MAP kinase를 부분분리 하였다. 이와 같이 얻은 MAP kinase를 가지고 면역 T세포에 존재하는 tyrosine kinase인 $p56^{lck}$ 의 N-terminal peptide로 구성된 GST-fusion protein에 대한 인산화를 확인하였다. 또한 세포에서 분리한 MAP kinase를 가지고 transcription factor의 하나인 c-Jun protein에 대한 인산화실험을 실시한 결과 MAP kinase에 의해 인산화 됨이 확인되었다. 이상의 결과를 통해 P388세포는 (1)세포 성장시 외부 신호를 G-protein-coupled receptor/protein kinase C/MAP kinase의 경로보다는 주로 tyrosine kinase receptor protein/Ras/MAP kinase의 경로를 이용하여 핵으로 전달하는 것으로 추측되 며 (2) mitogen의 처리로 활성화된 MAP kinase중 주로 42 Kd isoform이 핵내로 이동하고, 분리한 MAP kinase가 GST-fusion protein과 transcription factor인 c-Jun을 모두 인산화 시키는 결과로 보아 MAP kinase의 isoform에 따라 표적 compartment가 다르고 결과적으로 표적 기질에 차이가 있을지 모른다고 간접적으로 추론할 수 있다.

• Clinical and Experimental Reproductive Medicine
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• 제32권4호
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• pp.315-324
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• 2005

Objectives: To compare the efficacy of GnRH antagonist multiple dose protocol (MDP) with that of GnRH agonist long protocol (LP) in controlled ovarian hyperstimulation for in vitro fertilization in patients with high basal FSH (follicle stimulating hormone) level or old age, a retrospective analysis was done. Methods: Two hundred ninety four infertile women (328 cycles) who were older than 41 years of age or had elevated basal FSH level (> 8.5 mIU/mL) were enrolled in this study. The patients had undergone IVF-ET after controlled ovarian hyperstimulation using GnRH antagonist multiple dose protocol (n=108, 118 cycles) or GnRH agonist long protocol (n=186, 210 cycles). The main outcome measurements were cycle cancellation rate, consumption of gonadotropins, the number of follicles recruited and total oocytes retrieved. The number of fertilized oocytes and transferred embryos, the clinical pregnancy rates, and the implantation rates were also reviewed. And enrolled patients were divided into three groups according to their age and basal FSH levels; Group A - those who were older than 41 years of age, Group B - those with elevated basal FSH level (> 8.5 mIU/mL) and Group C - those who were older than 41 years of age and with elevated basal FSH level (> 8.5 mIU/mL). Poor responders were classified as patients who had less than 4 retrieved oocytes, or those with $E_2$ level <500 pg/mL on the day of hCG injection or those who required more than 45 ampules of exogenous gonadotropin for stimulation. Results: The cancellation rate was lower in the GnRH antagonist group than in GnRH agonist group, but not statistically significant (6.8% vs. 9.5%, p=NS). The amount of used gonadotropins was significantly lower in GnRH antagonist group than in agonist group ($34.8{\pm}11.3$ ampules vs. $44.1{\pm}13.4$ ampules, p<0.001). The number of follicles > 14 mm in diameter was significantly higher in agonist group than in antagonist group ($6.7{\pm}4.6$ vs. $5.0{\pm}3.4$, p<0.01). But, there were no significant differences in clinical pregnancy rate (24.5% in antagonist group vs. 27.4% in agonist group, p=NS) and implantation rate (11.4% in antagonist group vs. 12.0% in agonist group, p=NS) between two groups. Mean number of retrieved oocytes was significantly higher in GnRH agonist LP group than in GnRH antagonist MDP group ($5.4{\pm}3.5$ vs. $6.6{\pm}5.0$, p<0.0001). But, the number of mature and fertilized oocytes, and the number of good quality (grade I and II) and transferred embryos were not different between two groups. In each group A, B, and C, the rate of poor response did not differ according to stimulation protocols. Conclusions: In conclusion, for infertile women expected poor ovarian response such as who are old age or has elevated basal FSH level, a protocol including a controlled ovarian hyperstimulation using GnRH antagonist appears at least as effective as that using a GnRH agonist, and may offer the advantage of reducing gonadotropin consumption and treatment period. However, much work remains to be done in optimizing the GnRH antagonist protocols and individualizing these to different cycle characteristics.