• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1670-1680
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• 2017

Lignocellulose, composed mostly of cellulose, hemicellulose, and lignin generated through secondary growth of woody plant, is considered as promising resources for biofuel. In order to use lignocellulose as a biofuel, biodegradation besides high-cost chemical treatments were applied, but knowledge on the decomposition of lignocellulose occurring in a natural environment is insufficient. We analyzed the 16S rRNA gene and metagenome to understand how the lignocellulose is decomposed naturally in decayed Torreya nucifera (L) of Bija forest (Bijarim) in Gotjawal, an ecologically distinct environment. A total of 464,360 reads were obtained from 16S rRNA gene sequencing, representing diverse phyla; Proteobacteria (51%), Bacteroidetes (11%) and Actinobacteria (10%). The metagenome analysis using single molecules real-time sequencing revealed that the assembled contigs determined originated from Proteobacteria (58%) and Actinobacteria (10.3%). Carbohydrate Active enZYmes (CAZy)- and Protein families (Pfam)-based analysis showed that Proteobacteria was involved in degrading whole lignocellulose, and Actinobacteria played a role only in a part of hemicellulose degradation. Combining these results, it suggested that Proteobacteria and Actinobacteria had selective biodegradation potential for different lignocellulose substrates. Thus, it is considered that understanding of the systemic microbial degradation pathways may be a useful strategy for recycle of lignocellulosic biomass, and the microbial enzymes in Bija forest can be useful natural resources in industrial processes.

• 한국해양학회지
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• v.3 no.2
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• pp.63-72
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• 1968

해양에 있어서의 생물체의 에너지 순환, 즉 해양에 있어서의 생산체계에서 이중 먼저 기초생산체계만이라도 그 전상을 파악하자는 일은 먼 옛날부터 해양생물학자들의 진지하고도 끊임없는 추구대상이었다. 해양에는 막대한 양의 바닷물이 잠겨있고, 이곳에는 엄청난 양의 생명체가 함양되어 있으며, 이것들은 피포식자-포식자의 계열에서 어떠한 균형상태를 이루고 있는가하는 문제는 극히 흥미가 진진한 문제이다. 해양에 함양되는 무수한 생명체들은 간단없이 명멸, 소장만을 거듭하고 있다는 시적인 형용과 연상은 한층 더 구체화되어야 한다. 이러한 경향은 또한 근래의 세계인구의 격증에 대비하는 인간의 식량조달의 필요상, 해양이 지닌 잠재적인 식량생산의 여력을 재평가하자는 기운에서 한층 더 박차가 가해졌었다. 식량생산의 보고로서 해양에 주어진 책무는 크기만 하다. 해양에 잠겨있는 막대한 양의 바닷물은 긴 년월에 걸쳐 자연이 조제한 훌륭한 하나의 배양액이라고 불수 있고, 이것의 다각적인 이용개발은 지금부터 시작되었다고 해도 과언이 아니다. 근래에 이르러 각종기구 및 측기의 발달에 따라 해양의 물리, 화학 및 생물학적인 미세구조가 점차적으로 해명됨에 따라 해양학에 있어서의 고전적이고 전통적인 개념에 수정을 강요하는 사태가 적지않게 일어나고 있다. 해양의 기초생산체계에 대한 개념도 크게 비약하고 있으며, 해양에 있어서의 식물플랭크톤->동물플랭크톤->소형동물->어류를 연결하는 일방통행적인 식물연쇄개념을 크게 확대하고 비약시키지 아니하면 안될 여러 가지 사실이 들어나고 있다. 해양의 생물생산계의 기초적인 역할을 하는 것은 식물플랭크톤, 데트리타스 (detritus)등을 포함한 미소현탁물이라는 것은 틀림이 없으나, 이것들이 생명물질 기워니 아니드라도 물리현상으로 일어나는 기포를 매개로 하여 생성할수 있는 가능성이 제시되었다. 여기서는 이러한 해양의 미소현탁물에 관한 연구에 대해서 그 의의와 연구결과를 요약 소개하여 해양학도들의 참고로 한다. 이 종설을 꾸미는데 Jorgensen(1962), riley(1963), Parsons(1963), 서택(1966)등의 같은 토픽스에 관한 종설을 많이 참고로 한 것을 부기한다.

• Korean Journal of Transplantation
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• v.32 no.4
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• pp.104-107
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• 2018

Mucormycosis is an extremely rare but potentially life-threatening fungal infection. Gastrointestinal (GI) mucormycosis is very rare and occurs primarily in highly malnourished patients, especially in infants and children. A 55-year-old man with end-stage renal disease due to diabetic nephropathy, who had undergone deceased donor kidney transplantation 2 years prior, complained of abdominal pain and distension with a 3-day duration. Computed tomography revealed diffuse gastric wall thickening, and a huge amount of grey colored necrotic debris surrounded by erythematous erosive mucosa was observed at the antrum to upper body by GI endoscopy. The microscopic examination obtained from a GI endoscopic specimen demonstrated peptic detritus with numerous non-septate mucor hyphae in the mucosa and submucosa. Mucormycosis was diagnosed based on the clinical findings and morphological features. A total gastrectomy was performed and an antifungal agent was administered. A microscopic examination of the surgical specimen demonstrated invasive mucormycosis with numerous fungal hyphae with invasion into the mucosa to subserosa. The patient and graft were treated successfully by total gastrectomy and antifungal therapy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.419-427
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• 2023

This study, analyzed the stomach contents, stable isotope signatures (δ¹³C and δ¹⁵N) in muscle tissue, and potential food sources (particulate organic matter, Zostera marina, epiphytes, and zooplankton) of Sardinops melanostictus in the southern coast of Korea to evaluate its dietary changes across seasons (summer and autumn 2022). The diet of S. melanostictus predominantly comprised organic detritus (99%) regardless of the season. The isotope results showed that S. melanostictus had mean δ¹³C and δ¹⁵N values of -17.7±0.4‰ and 10.6±0.5‰, respectively and seasonal variations were observed (t-test, P=0.010, P=0.002). However, the isotopic mixing model showed that particulate organic matter was a major nutritional source for S. melanostictus in summer and autumn (88.7% and 90.2%, respectively). Based on the stomach contents and stable isotope signatures, these results may help improve the understanding of S. melanostictus and establish the management measures for their conservation.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.6
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• pp.750-763
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• 2018

This study conducted 10 sampling sites survey 4 times to determine the trophic structure and energy flow of marine ecosystems for Uljin marine ranching area, Korean East Sea from March to October 2013. Based on the ecological characteristics of biological species, one used the non-Metric Multidimensional Scaling method based on the similarity of species. A total of 19 classified species groups formed categories including, top predators, seabirds, large pelagic fishes, small pelagic fishes, rockfishes, pleuronectiformes, benthic fishes, semi-benthic fishes, cephalopods, benthic feeders, epifauna, bivalves, abalone, Cnidaria, zooplankton, benthic algae, microalgae, phytoplankton and detritus. The biomass, production/biomass, consumption/biomass, diet composition data of each species groups to input data used in Ecopath mode estimated the trophic structure and energy flow of marine ecosystems in the Uljin marine ranching area. One estimated each species groups on the trophic level from 1 to 5.687. The sum of all consumption was estimated at $229.7t/km^2/yr$ and the sum of all exports was as estimated $3,432.4t/km^2/yr$. Total system throughput was at $6,796.2t/km^2/yr$, and the sum of all production was estimated at $3,613.1t/km^2/yr$. Net system production according to these results was estimated at $3,490.3t/km^2/yr$ and total biomass (excluding detritus) was estimated at $167.3t/km^2/yr$ in the Uljin marine ranching area.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.1
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• pp.1-13
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• 2006

A field survey on the spatio-temporal distribution characteristics and origins of organic matter in surface sediments was carried out monthly at six stations in Gamak Bay, South Korea from April 2000 to March 2002. The range of ignition loss(IL) was $4.6{\sim}11.6%(7.1{\pm}1.6%)$, while chemical oxygen demand(CODs) ranged from $12.25{\sim}99.26mgO_2/g-dry(30.98{\pm}19.09mgO_2/g-dry)$, acid volatile sulfide(AVS) went from no detection(ND)${\sim}10.29mgS/g-dry(1.02{\pm}0.58mgS/g-dry)$, and phaeopigment was $6.84{\sim}116.18{\mu}g/g-dry(23.72{\pm}21.16{\mu}g/g-dry)$. The ranges of particulate organic carbon(POC) and particulate organic nitrogen(PON) were $5.45{\sim}23.24 mgC/g-dty(10.34{\pm}4.40C\;mgC/g-dry)$ and $0.71{\sim}2.99mgN/g-dry(1.37{\pm}0.58mgN/g-dry)$, respectively. Water content was in the range of $43.1{\sim}77.6%(55.8{\pm}5.6%)$, and mud content(silt+clay) was higher than 95% at all stations. The spatial distribution of organic matter in surface sediments was greatly divided between the northwestern, central and eastern areas, southern entrance area from the distribution characteristic of their organic matters. The concentrations of almost all items were greater at the northwestern and southern entrance area than at the other areas in Gamak Bay. In particular, sedimentary pollution was very serious at the northwestern area, because the area had an excessive supply of organic matter due to aquaculture activity and the inflow of sewage from the land. These materials stayed longer because of the topographical characteristics of such as basin and the anoxic conditions in the bottom seawater environment caused by thermocline in the summer. The tendency of temporal change was most prominently in the period of high-water temperatures than low-water ones at the northwestern and southern entrance areas. On the other hand, the central and eastern areas did not show a regular trend for changing the concentrations of each item but mainly showed a higher tendency during the low-water temperatures. This was observed for all but AVS concentrations which were higher during the period of high-water temperature at all stations. Especially, the central and eastern areas showed a large temporal increase of AVS concentration during those periods of high-water temperature where the concentration of CODs was in excess of $20mgO_2/g-dry$. The results show that the organic matters in surface sediments in Gamak Bay actually originated from autochthonous organic matters with eight or less in average C/N ratio including the organic matters generated by the use of ocean, rather than terrigenous organic matters. However, the formation of autochthonous organic matter was mainly derived from detritus than living phytoplankton, indicated the results of the POC/phaeopigment ratio. In addition, the CODs/IL ratio results demonstrate that the detritus was the product of artificial activities such as dregs feeding and fecal pellets of farm organisms caused by aquaculture activities rather than the dynamic of natural ocean activities.

• The Korean Journal of Ecology
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• v.25 no.5
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• pp.359-364
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• 2002

This study was conducted to examine the seasonal pattern of N and P uptake by reeds (Phragmites australis) planted in newly constructed Shihwa tidal freshwater marshes. Reed and soil samples were collected from the wetland periodically from June 2000 to May 2002. Reed samples were analyzed for dry weight and content of N and P Soil organic matter content and salinity were also determined. Dry matter content of reed increased during the growing season but decreased in the fall and winter. However, this seasonal pattern was not so evident in the second year. In particular, throughout the measurement period, dry matter content of reed was lowest at a site showing high soil salinity. Regression analyses between dry matter content of reed and soil EC(1:5) suggested that dry matter content per unit square meter would decrease by 1.5 kg with every 1 dS m/sup -1/ increase in soil EC(1:5). The amount of N and P assimilated by reed significantly decreased from the fall and was lowest in the spring. Net decrease in N content from reed during the fall and next spring was calculated as 34.5 and 24.6 g m/sup -2/ in the first and second years, respectively, while the corresponding P loss was 4.0 and 1.8 g m/sup -2/. Soil organic mailer content increased in the fall and winter, but decreased in the spring and summer. The results of this study suggested that the removal of N and P by reed would be considerable during the growing season but the nutrients taken up by reeds would return as detritus to the marshes in the fall and winter. Based on the results of the study, therefore, the harvest of the reed at the latter part of the growth would be recommended to prevent further water quality degradation. However, the long-term effects of reed harvest needs further study.

• Journal of the Korean earth science society
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• v.23 no.7
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• pp.565-574
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• 2002

Based on previous investigations of aerial photographs and topographical surveys, this study focuses on the sedimentologic features of the Daebudo area including sedimentation processes, sedimentary facies and hydrologic conditions of the erosional coast. A total of 137 surface sediments and one core (by hand auger) sediment were obtained to interpret the depositional environment of the erosional coast in the macro-tidal coast. Surface sediments are distributed from sandy gravel (sG) to silt (Z). Textural parameters are characterized not only by coarse, poorly sorted, positive skewed and multi-modal distribution in the supra-tidal flat, but also finer, relatively well-sorted, symmetric distribution in the intertidal flat. According to the C/M diagram, sediment transport modes of study area are characterized by the mixed mode of suspension and bedload in the upper-, middle-tidal flat and by uniform suspension in the lower-tidal flat due to tidal effect. Vertical sediment distribution of the core, collected near shoreline, shows coarsening-upward, poorly sorted pattern by the input of detritus resulting from coastal erosion. Considering the sedimentological features of the study area, it appears to be composed of a coastal zone changed by not only artificial reclamation, but also by natural processes such as strong wave action due to typhoons and storms during high water level and long/short-term sea level rising. As a result, tide-dominated erosional coasts show that the shore is affected by local, temporal and hydrological conditions near high tide level and that the intertidal flat is represented by a general tide-dominated sedimentary environment.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.362-372
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• 2011

The relationships among total epiphytes, algal epiphytes and eelgrass ($Zostera$ $marina$ L.) were studied at eelgrass medows from July, 1998 to July, 1999 in Yulim-ri, Yeosu, Korea. Epiphytic diatoms on eelgrass leaves were observed from July to December 1998. From the results of this study, we inferred the following three (3) conclusions : 1) As eelgrass grew older, biomass of epiphytes increased, according to relationships between the leaf length and area of eelgrass and biomass (DW, AFDW and Chl. $a$) of epiphytes. 2) According to the ratio of dry weight, ash-free dry weight and Chlorophyll of epiphytes, the algae with calcareous or siliceous skeletons, such as coralline algae or diatoms, were dominated in the epitphytic algae community. 3) The autotrophic index (AI) calculated from AFDW and Chl. $a$ of epiphytes varied from 151 to 375. However, the period of autotrophic community was shorter than heterotrophic community and the value of AI was high. From these results, we inferred that heterotrophic community, including detritus or microorganisms were dominated in the most of research period.

• The Korean Journal of Cytopathology
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• v.11 no.1
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• pp.1-10
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• 2000

The purpose of this study is to describe the cellular characteristics of endometrial hyperplasia without/with atypia in cervical smears. These cellular features were compared with those of normal endometrium and endometrial carcinoma. We reviewed 265 cervical smears : 64 normal proliferative endometrium, 118 endometrial hyperplasia without atypia, 21 endometrial hyperplasia with atypia, and 62 endometrial adenocarcinoma. Of these smears, 72(27.2%) smears which had diagnostic endometrial epithelial cells were selected for this study. The cytologic abnormalities about cellularity, background, changes in cellular architecture, alterations in nuclear size, anisokaryosis, chromatin pattern, nucleoli, cytoplasmic vacuoles, and mitosis were observed. Nuclear enlargement(1.6 to 2 times of the nucleus in the intermediate squamous cell) and anisokaryosis(${\geq}$2 fold in size variation) were highly suggestive of endometrial hyperplasia without/with atypia. The nuclei from endometrial hyperplasia with atypia were more coarsely granular in chromatin patterns than hyperplasia without atypia(33.3% vs 3.4%). Micronucleoli were observed in all endometrial conditions, but the presence of macronucleoli were more suggestive of hyperplasia with atypia(22.2%) and adenocarcinoma(55%). The changes in cellular architecture(loss of polarity, uneven internuclear distance, overlapping and loose arrangement) were seen in hyperplasia with atypia and adenocarcinoma. Characteristically, bloody background was seen in endometrial hyperpiasia, and cellular detritus or granular proteinaceous material was only observed in endometrial adenocarcinoma. Mitoses were also observed in adenocarcinoma. In conclusion, although there is no single parameter useful for the cytologic differential diagnosis of endometrial lesions, combined cytologic evaluation can be used to diagnose hyperplasia cytologically.