• The Korean Journal of Physiology and Pharmacology
• /
• v.20 no.6
• /
• pp.573-580
• /
• 2016

Caspases, a family of cysteine proteases, cleave substrates and play significant roles in apoptosis, autophagy, and development. Recently, our group identified 72 genes that interact with Death Caspase-1 (DCP-1) proteins in Drosophila by genetic screening of 15,000 EP lines. However, the cellular functions and molecular mechanisms of the screened genes, such as their involvement in apoptosis and autophagy, are poorly understood in mammalian cells. In order to study the functional characterizations of the genes in human cells, we investigated 16 full-length human genes in mammalian expression vectors and tested their effects on apoptosis and autophagy in human cell lines. Our studies revealed that ALFY, BIRC4, and TAK1 induced autophagy, while SEC61A2, N-PAC, BIRC4, WIPI1, and FALZ increased apoptotic cell death. BIRC4 was involved in both autophagy and apoptosis. Western blot analysis and luciferase reporter activity indicated that ALFY, BIRC4, PDGFA, and TAK1 act in a p53-dependent manner, whereas CPSF1, SEC61A2, N-PAC, and WIPI1 appear to be p53-independent. Overexpression of BIRC4 and TAK1 caused upregulation of p53 and accumulation of its target proteins as well as an increase in p53 mRNA levels, suggesting that these genes are involved in p53 transcription and expression of its target genes followed by p53 protein accumulation. In conclusion, apoptosis and/or autophagy mediated by BIRC4 and TAK1 may be regulated by p53 and caspase activity. These novel findings may provide valuable information that will aid in a better understanding of the roles of caspase-related genes in human cell lines and be useful for the process of drug discovery.

• Korean Journal of Mathematics
• /
• v.24 no.3
• /
• pp.369-374
• /
• 2016

In this paper, we study the hereditary convex radius of convex harmonic mapping $f(z)=f_1(z)+{\bar{f_x(z)}}$ under the integral operator $I_f(z)={\int_{o}^{z}}{\frac{f_1(u)}{u}}du+{\bar{{\int_{o}^{z}}{\frac{f_x(u)}{u}}}}$ and obtain the sharp constant ${\frac{{\sqrt[4]{6}}-{\sqrt[]{15}}}{9}}$, which generalized the result corresponding to the class of analytic functions given by Nash.

• Korean Journal of Applied Biomechanics
• /
• v.16 no.1
• /
• pp.101-108
• /
• 2006

The purpose of this study was to compare GRF characteristics during walking wearing jogging and roller shoes. Twelve male middle school students (age: $15.0{\pm}0.0\;yrs$, height: $173.6{\pm}5.0\;cm$, weight: $587.6{\pm}89.3\;N$) who have no known musculoskeletal disorders were recruited as the subjects. Kinematic data from six S-VHS camcorders(Panasonic AG456, 60 fields/s) and GRF data from two force platform; (AMII OR6-5) were collected while subjects walked wearing roller and jogging shoes in random order at a speed of 1.1 m/s. An event sync unit with a bright LED light was used to synchronize the video and GRF recordings. GRF data were filtered using a 20 Hz low pass Butterworth. digital filter and further normalized to the subject's body weight. For each trial being analyzed, five critical instants and four phases were identified from the recording. Temporal parameters, GRFs, displacement of center of pressure (DCP), and loading and decay rates were determined for each trial. For each dependent variable, paired t-test was performed to test if significant difference existed between shoe conditions (p <.05). Vertical GRFs at heel contact increased and braking forces at the end of initial double limb stance reduced significantly when going from jogging shoe to roller shoe condition. Robbins and Waked (1997) reported that balance and vertical GRF are closely related It seems that the ankle and knee joints are locked in an awkward fashion at the heel contact to compensate for the imbalance. The DCP in the antero-posterior direction for the roller shoe condition was significantly less than the corresponding value for the jogging shoe condition. Because the subjects tried to keep their upper body weight in front of the hip to prevent falling backward, the DCP for the roller shoe condition was restricted The results indicate that walking with roller shoes had little effect on temporal parameters, and loading and decay rates. It seems that there are differences in GRF characteristics between roller shoe and jogging shoe conditions. The differences in GRF pattern may be caused primarily by the altered position of ankle, knee, and center of mass throughout the walking cycle. Future studies should examine muscle activation patterns and joint kinematics during walking with roller shoes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.9
• /
• pp.1228-1234
• /
• 2000

The reported study was conducted on range managed Malpura ewes that were non-breeding empty, were at an advanced stage of pregnancy, and were in early lactation, under a protocol of free grazing with concentrate supplementation at 1.00, 1.25 and 1.50% of their body weight to assess their plane of nutrition and nutrient intake. The biomass yield of pasture plots was 1689, 1820 and 2912 kg/ha in pregnancy, lactation and empty phases, respectively. In addition to natural shrubs and forbs, Cenchrus ciliaris (36.4%) and dead litter (31.6%) were the major component of pasture vegetation during pregnancy. The dead litter disappeared during the lactation and empty phase with a concomitant increase in distribution of Cenchrus ciliaris to 73.0 and 87.2% respectively. The daily dry matter consumption from supplemental concentrate and free grazing was 70.1, 57.3 and 63.5 g/kg $W^{0.75}/d$ with concentrate to roughage ratio of 40:60, 47:53 and 33:67 in pregnancy, lactation and empty phases respectively. Digestibility of DM and OM were similar in the three phases while CP digestibility was higher (p<0.0l) during lactation than other two phases. Digestibility of NDF, ADF and cellulose were higher (p<0.0l) in empty than pregnancy and lactation, while hemicellulose digestibility was similar in lactation and empty and lower in pregnancy phase. The ewes in ~hases of pregnancy, lactation and empty consumed 7.1, 7.7 and 6.1 g DCP and 197.2, 214.6 and 232.5 kcal DE/kg $W^{0.75}/d$ respectively. It is concluded that ewes maintained on semi-arid Cenchrus dominated pasture with concentrate supplementation during pregnancy, lactation and empty phases consumed 45.2, 45.1 and 35.2 g DCP/Mcal ME respectively.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.9
• /
• pp.1228-1232
• /
• 2001

Forty five Soviet Chinchilla rabbits weaned at 35 d of age were fed 5 experimental diets prepared by using oat plant meal ($T_2,\;T_4$) and tall fescue hay ($T_3,\;T_5$) at 25% and 50% level along with control ($T_1$). The gain in body weight at 84 d of age decreased with test material substitution, however, the differences were non significant when compared with oat plant meal and were significant (p<0.05) with tall fescue hay at both the levels. Feed conversion ratio also deteriorated with test material and its increasing proportion and the differences were significant (p<0.05). The performance-index calculated were 42.1, 36.7, 29.4, 33.3 and 18.8 in $T_1,\;T_2,\;T_3,\;T_4$ and $T_5$, respectively. Digestibility of all the organic nutrients decreased significantly (p<0.05) with test material incorporation, as a result nutritive value of the test diets decreased linearly. Nitrogen balance as per cent of intake was higher in oat plant meal substituted diet compared to tall fescue substituted diet. Cost per kg live weight gain was lowest with 50% oat plant meal followed by 25% oat plant meal incorporation. It was highest with tall fescue hay substitution. Nutritive value of oat plant meal as calculated was DCP 5.1%, TDN 35.8% and that of tall fescue hay was DCP 6.5%, TDN 30.8%. Thus, oat plant meal can be substituted safely up to 25% in the feeding regime of weaner rabbits whereas, for tall fescue hay the safe limit is below 25% level and needs to be ascertained.

• Journal of Korea Technology Innovation Society
• /
• v.5 no.1
• /
• pp.44-58
• /
• 2002

There have been many solutions to overcome theoretical problems of the Discounted Cash Flow Methods, especially on the valuation of technology. Real Options are thought as a solution. There, however, are another problems in applying Real Options for the valuation of technology; diversity and complexity of models. This Paper recommends 5 models for the valuation of technology, especially for practitioners.

• Fire Science and Engineering
• /
• v.14 no.2
• /
• pp.33-38
• /
• 2000

Flux, essentially used in soldering process of PCB (Printed Circuit Board) in electronics industry, contains IPA (Isopropyl alcohol) and methanol, which are highly inflammable and explosive. Hazard Chemical Controlling Law classified methanol as toxic material and Environmental Law classified methanol as VOC (Volatile Organic Compound). So there have been pressing needs of developing substitutes for the existing Flux. New solvent which is non-flammable and main component is DCP having same specific character of the existing Flux. It's been combirated with proper composition ratio adding stabilizer. As a result, it relieved working Environment Allowance thickness 200 ppm to 470 ppm, chance of not been soldered 0.083% to 0%, spread 85% to 87%, power saving resistance 1.0$\times$$10^{12}$$\Omega$ to 6.9$\times$$10^{12}$$\Omega$, which means a lot better than the existing Flux. Therefore, Flux confirmed the chance of improving productivity, safety, environment safety and quality. Also, Flux got a satisfied result after product quality test and product reliability test.

• Elastomers and Composites
• /
• v.50 no.1
• /
• pp.24-29
• /
• 2015

In this study, we investigated the effects of a crosslinking agent and a compatibilizer on the mechanical and rheological properties of waste PP and waste ground rubber tire (WGRT) composites. In order to simulate a commercial TPV, the component of waste PP and WGRT was fixed at 30 and 70 wt%, respectively. With the simple addition of SEBS-g-MA into the waste PP/WGRT composites, the tensile strength of the composite was decreased, whereas both the elongation at break and impact strength were significantly increased because of rubbery characteristics of SEBS-g-MA. In order to further improve the properties of the composites, the waste PP/WGRT/SEBS-g-MA composites was revulcanized with dicumyl peroxide (DCP). As expected, mechanical properties of the revulcanized composites was generally improved. Especially, with 15 and 1 phr of SEBS-g-MA and DCP, elongation at break was highest value of about 183% because of the recross-linking of WGRT without chain scission of the main chain. It was found that complex viscosity of the revulcanized composite increased which might verify further vulcanization of the WGRT.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.2
• /
• pp.200-205
• /
• 2001

The effects of dietary supplementation with the sodium salt of isobutyric acid in a low protein (10% CP) wheat straw based diet on nutrient utilization, blood metabolites and growth performance were studied with male crossbred calves. The calves were divided into two equal groups of 6 each. The animals of the control group were fed a basal diet consisting of wheat straw, concentrate mixture and green oat fodder in 40:40:20 proportion whereas BCFA supplemented group received the basal diet+isobutyric acid at 0.75 percent of basal diet. The duration of study was 120 days. The feed intake between experimental groups did not differ significantly and the average total DMI (% BW) was 1.99 and $1.95kg\;day^{-1}$ in control and BCFA supplemented diets. The dietary supplementation of BCFA improved (p<0.01) the DM, OM, CP (p<0.05), NDF and cellulose digestibilities by 8.50, 9.01, 5.39, 17.78 and 18.44 per cent over those fed control diet. The total N retention on BCFA supplementation was improved (p<0.01) due to the decreased (p<0.05) faecal N excretion. The BCFA supplementation did not alter the blood circulatory levels of glucose, total protein, albumin, urea N and amino acids. However after 120 days of experimental feeding a significant (p<0.05) increase in the concentrations of non-esterified fatty acid was observed in control group. The DCP intake and the DCP content of experimental diets was similar in both groups. However, the TDN content of BCFA supplemented diet was significantly (p<0.01) higher (64.35%) than that of control (59.60%). The total live weight gain in BCFA supplemented diet increased by 15.94% over control. The average daily gain and efficiency of feed conversion were also improved in BCFA fed calves by 13.38 and 26.71% respectively, compared to control. It is concluded that dietary supplementation with isobutyric acid improved the digestibility of nutrients and growth performance of calves.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.6
• /
• pp.769-776
• /
• 2004

Data from 24 feeding trials conducted on lactating cattle from different institutes across India were subjected to regression analysis to derive requirements of ME, TDN, CP and DCP for maintenance, milk production and body weight gain. Maintenance requirements for ME, TDN, CP and DCP were 598 KJ, 39.5 g, 6.27 g and 2.90 g/kg $W^{0.75}$, respectively and the corresponding requirements for production of 1 kg 4% FCM were 5,023 KJ, 332 g, 82 g and 58 g. The corresponding requirements for one g gain in BW were 27 KJ, 1.78 g, 0.44 g and 0.19 g. Regression equations had high $R^{2}$ values (0.67 to 0.90) and the equations (Fvalue) as well as coefficients were highly significant (p<0.001). Regressed values were used to develop feeding standards. Derived values matched well with the actual intake versus performance of animals under diverse feeding conditions. The new standards so derived predicted requirements and intake of nutrients for different production levels better than existing feeding standards; as these are based on a more thorough analysis of a larger database, the new feeding standards will be appropriate for wide use in India.