• Title/Summary/Keyword: DCN

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Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide (인간치수세포에 Mineral Trioxide Aggregate와 수산화칼슘 제재 적용 시 유전자 발현 양상 비교)

  • Kim, Yong-Beom;Shon, Won-Jun;Lee, Woo-Cheol;Kum, Kee-Yeon;Baek, Seung-Ho;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.36 no.5
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    • pp.397-408
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    • 2011
  • Objectives: This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs). Materials and Methods: wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction. Results: Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal. Conclusions: Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

Determination of Correlation between Cetane Number, Derived Cetane Number and Cetane Index for Diesel Fuel (경유연료의 세탄가, 유도세탄가 및 세탄지수의 상관관계 분석)

  • Jeon, Hwayeon;Kim, Ji Yeon;Kim, Shin;Yim, Eui Soon
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1134-1144
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    • 2018
  • Cetane Number is one of the quality standard for diesel, which assesses the compatibility of ignition quality of diesel compression in diesel engines. Cetane number must be upper 52 to keep the recent diesel quality standards. It is known that if cetane number is high, there will be shorter ignition delay periods than being lower. On the other hands, if cetane number is too high that exceeds the quality standard, there will increase the air pollution and decrease of the fuel efficiency because incomplete combustion. In South Korea, various methods are being used to measure the cetane number such as cetane number that used CFR engine, cetane index from calculate density and distillation temperature and derived cetane number to make up for CFR engine that ignition delay in high temperature is implemented. In this study will be conducted by collecting the diesel from the major oil companies, and try to analyze the correlation between the different methods of cetane number with various factors. At the results of this study, it was shown that the cetane index is high then cetane engine and derived cetane number. therefore it will be necessary to additional research for out of cetane number quality standards.