• Journal of the Korean Institute of Gas
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• v.21 no.3
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• pp.61-69
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• 2017

The commercial development of unconventional gas is pursued in North America because it is more feasible owing to the technology required to improve productivity. Shale reservoir have low permeability and gas production can be carried out through cracks generated by hydraulic fracturing. The decline rate during the initial production period is high, but very low latter on, there are significant variations from the initial production behavior. Therefore, in the prediction of the production rate using deterministic decline curve analysis(DCA), it is not possible to consider the uncertainty in the production behavior. In this study, production rate of the Eagle Ford shale is predicted by Arps Hyperbolic and Modified SEPD. To minimize the uncertainty in predicting the Estimated Ultimate Recovery(EUR), Monte Carlo simulation is used to multi-wells analysis. Also, kernel density function is applied to determine probability distribution of decline curve factors without any assumption.

• BMB Reports
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• v.54 no.11
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• pp.563-568
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• 2021

Cancer cells predominantly generate energy via glycolysis, even in the presence of oxygen, to support abnormal cell proliferation. Suppression of PDHA1 by PDK1 prevents the conversion of cytoplasmic pyruvate into Acetyl-CoA. Several PDK inhibitors have been identified, but their clinical applications have not been successful for unclear reasons. In this study, endogenous PDHA1 in A549 cells was silenced by the CRISPR/Cas9 system, and PDHA1^WT and PDHA1^3SD were transduced. Since PDHA1^3SD cannot be phosphorylated by PDKs, it was used to evaluate the specific activity of PDK inhibitors. This study highlights that PDHA1^WT and PDHA1^3SD A549 cells can be used as a cell-based PDK inhibitor-distinction system to examine the relationship between PDH activity and cell death by established PDK inhibitors. Leelamine, huzhangoside A and otobaphenol induced PDH activity-dependent apoptosis, whereas AZD7545, VER-246608 and DCA effectively enhanced PDHA1 activity but little toxic to cancer cells. Furthermore, the activity of phosphomimetic PDHA1 revealed the complexity of its regulation, which requires further in-depth investigation.

• Korean Journal of Environment and Ecology
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• v.26 no.3
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• pp.426-436
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• 2012

This study was carried out to investigate actual vegetation, the structure of plant community, and ecological succession sere of coastal forest ecosystem in warm temperate climate zone, Taejongdae, Busan City, Korea to provide the basic data for planning of the forest management. As a result of analysis of actual vegetation, vegetation types divided into 35 types, and the area of survey site was $1,750,461m^2$. The ratio of vegetation type dominated by Pinus thunbergii was 80.7%, dominated by Quercus spp. was just 5.0%, and dominated by Carpinus tschonoskii was just 0.4%. Eighteen plots(size is $20m{\times}20m$) were set up and the results analyzed by DCA which is one of the ordination technique showed that the plant communities were divided into four groups which are community I(P. thunbergii community), community II(P. thunbergii-Quercus serrata community), community III(Q. serrata-P. thunbergii community), and community IV(Carpinus tschonoskii-P. thunbergii community). The age of community I was from 38 to 59 years old, that of community II was from 35 to 71 years old, that of community III was from 37 to 53 years old, that of community IV was from 50 to 72 years old, thus we supposed that the age of the study site is about from 38 to 72 years old. We supposed that the successional sere of the study site is in the early stage of ecological succession in the warm temperate climate zone. The dominant species will be changed from P. thunbergii to Q. serrata or Carpinus tschonoskii in the canopy layer, on the other hand, Eurya japonica will be dominant species in the understory layer, and E. japonica and Trachelospermum asiaticum var. intermediumwill be dominant species in the shrub layer for a while. According to the index of Shnnon's diversity(unit: $400m^2$), community I ranged from 0.8640 to 1.3986, community II was from 0.1731 to 1.1885, community III was from 0.8250 to 1.0042, and community IV was from 0.3436 to 0.6986.

• Korean Journal of Environment and Ecology
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• v.27 no.2
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• pp.241-252
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• 2013

This study investigated plant community structure of Dongbaekdongsan(Mt.) in Jeju-do to identify character as evergreen broad-leaved forest. 20 plots(size is $20m{\times}20m$) were set up and plant communities were divided into 5 communities of Quercus glauca-Castanopsis cuspidata var. sievoldii-Ilex integra(Camellia japonica), Quercus glauca-Castanopsis cuspidata var. sievoldii-Camellia japonica, Quercus glauca-Camellia japonica, Quercus glauca-Camellia japonica-Pinus thunbergii and Pinus thunbergii-Quercus glauca by using TWINSPAN, DCA ordination, mean importance value and environmental condition. Area of Evergreen broad-leaved forest with stonny ground is covered with Quercus glauca and Castanopsis cuspidata var. sievoldii in canopy layer and Camellia japonica in the subordinate layer. Area of remaining soil's depth is covered with Pinus thunbergii. It has been alleged that Pinus thunbergii planted by human when Dongbaekdongsan(Mt.) is not selected local monuments. All of communities is predicted success to community of Quercus glauca-Camellia japonica. Shannon's diversity ranged from 1.0268 to 1.0717 in area of Evergreen broad-leaved forest and 1.2102 in area of Pinus thunbergii. 10 species of broad-leaved tree's constancy ratio is over the 80%. In future, Castanopsis cuspidata var. sievoldii and Cinnamomum japonicum can cover canopy layer, but it has low importance value. Specially high aged Camellia japonica is appeared everywhere. It has been alleged that it was touched by divinity since past. Dongbaekdongsan(Mt.) has different plant structure with another evergreen broad-leaved forest. It is hard that knowing its successional sere because it was damaged when it isn't degignate local monument. Accordingly continuous monitoring was required, successional direction and community's character is identified after forest become steady.

• Journal of Korean Society of Forest Science
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• v.109 no.1
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• pp.1-22
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• 2020

In order to evaluate the vertical distribution and distributional pattern of vascular plants in the Samga district of Sobaeksan National Park, vascular plants were surveyed along a hiking trail from the Samga Tour Support Center to the top of a mountain. The elevation range was divided into 11 sections with 100 m intervals from 400 m to 1439 m above sea level.A total of 375 taxa were listed, comprising 92 families, 235 genera, 332 species, 3 subspecies, 37 varieties, and 3 forms. The pattern of species richness along the elevational gradient showed a reverse hump-shaped trend. The species distribution pattern was positively correlated with the soil exchangeable cations Ca²⁺ and Mg²⁺, soil pH, available phosphate, and the warmth index. Furthermore, slope, soil moisture content, and soil exchangeable cations were significantly correlated with species distribution. DCA grouped herb species into two groups. Stands of each section were sequentially arranged from 400 m to 1500 m along an altitudinal gradient. Soil moisture content, soil pH, soil K²⁺ and Na²⁺, available phosphate, and slope were significantly correlated with stand distribution. This study provides important data that could be useful for conservation and the sustainable use of biodiversity in the study area. In order to understand the ecological and environmental characteristics and distribution of plant species, it will be necessary to continuously develop relative studies with continuous monitoring.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.72-81
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• 2012

We investigated the effects of acid mine drainage (AMD) from abandoned coal mines on benthic macroinvertebrate communities in the upper reaches of the Nakdong River from May to October in 2009. Qualitative and quantitative sampling (Surber sampler: $50{\times}50$ cm; mesh size, 0.2 mm) was conducted at 7 study sites and 3 control sites in the study area. We thus sampled 117 species belonging to 53 families, 15 orders, 6 classes, and 5 phyla; the Ephemeroptera, Plecoptera, and Trichoptera group (EPT-group) represented the majority of the benthic macroinvertebrate community (71 species; 64.5%). In the quantitative sampling, a total of 11,575 individuals belonging to 58 species of benthic maroinvertebrates were sampled from the study sites (Sites 1-7), whereas 2,844 individuals belonging to 79 species were sampled from the control sites (Sites A-C). Tolerant species such as oligochaetes, $Epeorus$ $pellucidus$, $Baetis$ $fuscatus$, Hydropsychidae species, and Chironomidae species were predominant in the study sites. The community indices for the study sites, such as MacNaughton's dominance index (DI) (mean${\pm}$SD, $0.52{\pm}0.21$; range, 0.33-0.85) and the Shannon diversity index ($H^{\prime}$) ($2.06{\pm}0.60$; 1.06-2.57), were different from those for the control sites (DI: $0.29{\pm}0.07$, 0.22-0.35; $H^{\prime}$: $3.13{\pm}0.14$; 3.03-3.30). In the study sites, shredders and scrapers were scarce, whereas gathering-collectors (mainly Chironomidae species) were relatively abundant, as were clingers and burrowers. The detrended correspondence analysis (DCA) and similarity analyses showed that benthic macroinvertebrate communities in the study sites were clearly separated from those of the control sites, with the greatest dissimilarity being noted at the uppermost study site (Site 1), which is located close to an abandoned coal mine. The Korean saprobic index (KSI) and the ecological score using benthic macroinvertebrates (ESB) showed that the uppermost study site (Site 1) was ${\alpha}$-mesosaprobic or heavily polluted, whereas other study sites were in a fair or relatively good condition.

• Korean Journal of Environment and Ecology
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• v.29 no.5
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• pp.764-776
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• 2015

This study aims to identify the structure of the plant community, and the ecological succession sere and the change in the forest ecosystem in Jungdaesa-Birobong area, Odaesan National Park_(i._e., located at high altitudes(over 1,000m)). It seeks to offer the basic data for the planning of vegetation management. In order to verify the status of the forest vegetation between Jungdaesa-Birobong, seventeen plots(size is $20m{\times}20m$) were set up as research sites at high altitudes. Importance value, distribution by diameter at breast height(DBH), the growth volume and age of the sample trees, similarity index and species diversity index of each survey plot were analysed. According to the results of DCA(Detrended Correspondence Analysis), one of the multivariate statistical techniques. It was found that the plant communities were classified into five groups: community I_(Quercus mongolica-Tilia amurensis community), community II_(Q. mongolica-Deciduous broad-leaved community), community III_(Q. mongolica-Pinus koraiensis community), community IV_(Abies holophylla-Q. mongolica community) and community V_(A. holophylla-Deciduous broad-leaved community). Community I which is dominated by Quercus mongolica and Deciduous broad-leaved communities is located at an altitude of over 1,300 meters(ranging from 1,335m to 1,495m), the community IV and V which are dominated by Abies holophylla are located at an altitude of under 1,200 meters(ranging from 1,115m to 1,175m) and the community II and III which include the main species of Quercus mongolica, Pinus koraiensis and Abies holophylla are located at an altitude of between 1,160 meters and 1,300 meters. The results showed that Quercus mongolica tends to have a higher importance value of woody species at a higher altitude while Abies holophylla tends to have higher importance value at a lower altitude. For the importance value woody species and -DBH class distribution, the communites I, II and III are expected to continuously maintain the present status. Whereas, for the influence of communities IV and V, Q. mongolica is predicted to be weakened. The age of sample trees was between 85 and 161; the average age was 123. The index of Shannon's Species diversity (H') showed heterogeneity was found among community I_(i._e., located at high altitude) and communities IV and V_(i._e., located at low altitude). As a results of analysing the index of Shannon's Species diversity (H': unit: $400m^2$), community III showed the highest diversity intex with 1.1109 followed by community II with 1.0475, community I with 1.0125, community IV with 0.9918 and community V with 0.8686. This study verified that the index of Shannon's species was significantly different by plant communities. For instance, when comparing the index of Shannon's species diversity in Quercus mongolica communities of this study and that of past relevant research, the value of index is very similar. However, the diversity index for the community which is dominated by Abies holophylla showed lower value when compared to the results from past relevant research.

• Korean Journal of Ecology and Environment
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• v.50 no.4
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• pp.381-402
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• 2017

In order to investigate distribution of vascular plants along elevational gradient in the Nodong valley of Gyebangsan, vascular plants of eight sections with 100-meter-high were surveyed from the Auto-camping site (800 m) to the top of a mountain (1,577 m). There were a total of 382 taxa: 89 families, 234 genera, 339 species, 7 subspecies, 34 varieties, and 2 forms. As a result of analyzing the pattern of species richness, it showed a reversed hump-shaped with minimum richness at mid-high elevation. As a result of analyzing habitat affinity types, the proportion of forest species increased with increasing elevation. But, the ruderal species decreased with increasing elevation, and then increased at the top of a mountain. As for the proportion of life forms, the annual herbs gradually decreased with increasing elevation, but it did not appear between 1,300 m and 1,500 m and then increased at the top of a mountain. The trees gradually increased with elevation and decreased from 1,300~1,400 m. The vascular plants divided into four groups by using DCA. The arrangement of each stands was arranged in order from right to left on the I axis according to the elevation. The distribution of vascular plants is determined by their own optimal ranges of vegetation. Also, rise in temperature due to climate change affects the distribution of vascular plants, composition, and diversity. Therefore, continuous monitoring is necessary to confirm ecological and environmental characteristics of vegetation, distribution ranges, changes of habitat. Furthermore, plans for conservation and management based on these data should be prepared according to climate change.

• Korean Journal of Environment and Ecology
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• v.27 no.5
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• pp.579-591
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• 2013

This study was carried out to investigate the actual vegetation, the structure of plant community, and ecological succession sere of forest ecosystem in temperate northern climate zone, Daegwallyeong Ranch, Gangwon-do (Province) and to offer the basic data for planning of the forest managemant. As a result of analysis of actual vegetation, vegetation types divided into 56types and the area of survey site was $19,397,361m^2$. The ratio of vegetation type dominated by Quercus mongolica forest was 39.1%, primary grassland was 24.7%, Quercus mongolica-Deciduous broad-leaved forest was 11.3%. Twenty eight plots (size is $20m{\times}20m$) were set up and the results analyzed by DCA which in one of the ordination technique showed that the plant communities were divided into six groups which area community I (Pinus densiflora-Quercus mongolica community), community II (Quercus mongolica-Pinus densiflora community), community III (Quercus mongolica community), community IV (Quercus mongolica-Deciduous broad-leaved community), community V (Deciduous broad-leaved community), community VI (Sorbus alnifolia community). The age of community Iwas ranged from 57 to 62 years old, that of community IIwas ranged from 41 to 77 years old, community III was ranged from 47 to 108 years old, community IV was ranged from 47 to 82 years old, community V was 47 years old, community VI was 55 years old, thus we supposed that the age of the study site is about from 41 to 108 years old. The Ecological succession is predicted from Pinus densiflora community to Quercus mongolica community and Deciduous broad-leaved were distributed in the center of the valley in Daegwallyeong Ranch. According to the index of Shannon's diversity (unit: $400m^2$), community IV was ranged from 0.8203 to 1.1439, community III was ranged from 0.8019 to 1.1375, community V was 1.0993, community I was ranged from 0.9475 to 1.0797, community II was ranged from 0.6896 to 1.0324, community VI was 0.9909.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.46-56
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• 2012

Benthic macroinvertebrates were investigated in Hoeongseong Lake region from March to October 2010, Korea. Macroinvertebrate communities, composition of the functional feeding groups, habitiat oriented groups and the biological water quality were assessed above and below Hoeongseong Dam in the lake region. Six sites, two (St.1~2) above the lake, two (St. 3~4) into the lake, and two (St. 5~6) below the dam, were selected for quantitative (Surber sampler $30cm{\times}30cm$, mesh size 0.2 mm) and qualitative (Hand net) samplings of benthic macroinvertebrates. As a result, a total of 83 species belonging to 43 families, 17 orders, 8 classes, and 5 phyla were recognized. The EPT-group (50 spp. : 60.24%) that is major taxa or EPT-group plus Diptera (61 spp. : 73.49%) occupied most of benthic macroinvertebrates community. Based on quantitative sampling, the number of benthic maroinvertebrates above the lake was 2,399 individuals including 54 species, 28 families, 11 orders, 4 classes, and 4 phyla, whereas 510 individuals including 16 species, 12 families, 7 orders, 4 classes, and 3 phyla in the lake and 626 individuals including 62 species, 33 families, 13 orders, 6 classes, and 4 phyla below the dam were collected respectively. Dominance index was the highest, with 0.82-0.93 ($0.87{\pm}0.05$) in Hoeongseong lake (St. 3-4), diversity index was 3.04-3.16 ($3.10{\pm}0.06$), evenness index was 0.79-0.85 ($0.82{\pm}0.03$), and richness index was 7.27-8.52 ($7.90{\pm}0.63$), which were relatively higher below the dam sites. In the functional feeding groups, collector-gatherers and collector-filterers were the highest in the whole sites, and predators (Micronecta sedula) was appeared highly in the lake. Moreover, swimmers, burrowers, and clingers were considerably occupied in all collecting sites. The result of the DCA, similarity analysis, and MRPP were well reflective of the composition of lake and stream macroinvertebrates. ESB indicate that the lake sites were evaluated heavily polluted under priority improvement waters. Also, Semisulcospira gottschei, Ecdyonurus kibunensis, Epeorus pellucidus, Rhoenanthus coreanus, Stenelmis sp., and Cheumatopsyche brevilineata are considered as an indicator species above and below Hoeongseong Lake, whereas Macrobrachium nipponense and Micronecta sedula are indicated in the lake.