• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
• /
• pp.170.3-171
• /
• 2002

• 한국고분자학회지:고분자과학과기술
• /
• 제6권1호
• /
• pp.16-21
• /
• 1995

• 한국영양학회:학술대회논문집
• /
• 한국영양학회 2001년도 춘계연합학술대회 초록
• /
• pp.259.2-259
• /
• 2001

• Bulletin of the Korean Chemical Society
• /
• 제7권6호
• /
• pp.490-491
• /
• 1986

• Bulletin of the Korean Chemical Society
• /
• 제15권2호
• /
• pp.168-169
• /
• 1994

• 한국동물생명공학회지
• /
• 제34권4호
• /
• pp.322-322
• /
• 2019

• The Plant Pathology Journal
• /
• 제33권1호
• /
• pp.95-100
• /
• 2017

Fungicide-resistant Alternaria alternata impede the practical control of the Alternaria diseases in crop fields. This study aimed to investigate cytological fungicide resistance mechanisms of A. alternata against dicarboximide fungicide iprodione. A. alternata isolated from cactus brown spot was cultured on potato-dextrose agar (PDA) with or without iprodione, and the fungal cultures with different growth characteristics from no, initial and full growth were observed by light and electron microscopy. Mycelia began to grow from one day after incubation (DAI) and continued to be in full growth (control-growth, Con-G) on PDA without fungicide, while on PDA with iprodione, no fungal growth (iprodione-no growth, Ipr-N) occurred for the first 3 DAI, but once the initial growth (iprodione-initial growth, Ipr-I) began at 4-5 DAI, the colonies grew and expanded continuously to be in full growth (iprodione-growth, Ipr-G), suggesting Ipr-I may be a turning moment of the morphogenetic changes resisting fungicidal toxicity. Con-G formed multicellular conidia with cell walls and septa and intact dense cytoplasm. In Ipr-N, fungal sporulation was inhibited by forming mostly undeveloped unicellular conidia with degraded and necrotic cytoplasm. However, in Ipr-I, conspicuous cellular changes occurred during sporulation by forming multicellular conidia with double layered (thickened) cell walls and accumulation of proliferated lipid bodies in the conidial cytoplasm, which may inhibit the penetration of the fungicide into conidial cells, reducing fungicide-associated toxicity, and may be utilized as energy and nutritional sources, respectively, for the further fungal growth to form mature colonies as in Ipr-G that formed multicellular conidia with cell walls and intact cytoplasm with lipid bodies as in Con-G.

• 대한한방소아과학회지
• /
• 제29권1호
• /
• pp.1-14
• /
• 2015

Objectives This experimental study was designed to investigate the effects of Dai-saiko-to (DSH) on differentiation of 3T3-L1 preadipocytes and body weight, serum lipid levels in high-fat diet-induced obese mice. Materials and Methods Cells were incubated with DSH at an indicated concentration (0.01-1 mg/ml) for 24h, then the growth rate was assessed by MTS assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of DSH. On Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) and cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancer-binding proteins ${\alpha}$ ($C/EBP{\alpha}$) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. Four-weeks old mice (wild-type C57BL/6) were used for all experiments. Body weight gain and serum lipid levels were measured in the obesity-induced mice. Results DSH did not show toxicity even at the concentration of 1 mg/ml and DSH significantly inhibited the differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. Also, DSH significantly reduced the expressions of $PPAR{\gamma}$ and $C/EBP{\alpha}$ in a dose-dependent manner. Furthermore, DSH significantly reduced body weight gain, serum glucose, total cholesterol and LDL-cholesterol contents in obesity-induced mice. Conclusions These results demonstrated that DSH inhibited 3T3-L1 preadipocyte differentiations and high-fat diet-induced obesity in mice.

• The Plant Pathology Journal
• /
• 제36권5호
• /
• pp.450-458
• /
• 2020

Pepper and tomato plants infected with two Clavibacter species, C. capsici and C. michiganensis have shown different patterns of disease development depending on their virulence. Here, we investigated how pepper and tomato plants respond to infection by the high-virulent or low-virulent Clavibacter strains. For this, we chose two strains of each Clavibacter species to show different virulence level in the host plants. Although low-virulent strains showed less disease symptoms, they grew almost the same level as the high-virulent strains in both plants. To further examine the response of host plants to Clavibacter infection, we analyzed the expression patterns of plant defense-related genes in the leaves inoculated with different strains of C. capsici and C. michiganensis. Pepper plants infected with high-virulent C. capsici strain highly induced the expression of CaPR1, CaDEF, CaPR4b, CaPR10, and CaLOX1 at 5 days after inoculation (dai), but their expression was much less in low-virulent Clavibacter infection. Expression of CaSAR8.2 was induced at 2 dai, regardless of virulence level. Expression of GluA, Pin2, and PR2 in tomato plants infected with high-virulent C. michiganensis were much higher at 5 dai, compared with mock or low-virulent strain. Expression of PR1a, Osmotin-like, Chitinase, and Chitinase class 2 was increased, regardless of virulence level. Expression of LoxA gene was not affected by Clavibacter inoculation. These results suggested that Clavibacter infection promotes induction of certain defense-related genes in host plants and that differential expression of those genes by low-virulent Clavibacter infection might be affected by their endophytic lifestyle in plants.

• 대한의용생체공학회:의공학회지
• /
• 제19권6호
• /
• pp.603-609
• /
• 1998

가속도 변화에 따른 뇌의 미만성 부상을 해석하기 위하여 성인 및 2세, 6세아의 머리 부분에 대한 유한 요소 모델을 개발하였다. 외력은 최대값이 200g인 삼각형 형태의 가속도를 가하였으며, 가속도의 방향, 지속시간에 따른 변화를 해석하였다. 가속도 변화에 따라 발생되는 뇌내의 전단력 분포는 뇌간, 뇌교 및 중뇌등 신경조직이 밀집된 곳에서 크게 발생되어 이곳에서 미만형 부상이 발생할 확률이 높음을 알 수 있었으며, 특히 6세아 모델의 경우 뇌간에서의 최대 전단력이 굴전 형태의 회전가속도 받았을 때 가장 크게 나타나는 결과를 보여 개발된 모델이 임상결과와 일치함을 보여주고 있었다. 가속도 지속 시간이 길어짐에 따라 뇌내에 발생되는 압력 및 최대 전단력의 크기가 증대되고 있었으며, 유아모델의 경우 성인모델에 비하여 가속도 방향과 관계없이 낮은 압력이 발생하였지만 발생압력이 감소하지 않고 지속되는 현상을 보이고 있었다. 그리고 각 가속도에 의한 미만성 부상을 예방하기 위한 안전지수로는 현재 탑승자의 안전 설계에 활용되고 있는 HIC보다는 최대 전단응력이 더 적절한 부상 예측인자임을 알 수 있었다.