• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.5069-5074
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• 2012

Objectives: The purpose of this study was to determine the relationship between methylation status of the Dact1 gene and MTHFR a1298c polymorphic forms in transitional cell carcinoma tissues in a Chinese population. Methods: Polymorphisms of folate metabolism enzyme gene MTHFR were assessed by restrictive fragment length polymorphism (RFLP) methods and PCR-based DNA methylation analysis was used to determine the CpG island methylation status of the Dact1 gene. Associations between the methylation status of the Dact1 gene and clinical characteristics, as well as MTHFR a1298c polymorphisms, were analyzed. Results: aberrant methylation of the Dact1 gene was found in 68.3% of cancer tissues and 12.4% of normal tissues,. The methylation rate of the Dact1 gene in cancer tissues was significantly higher in patients with lymph node metastasis than in those without lymph node metastasis (46.3% vs. 17.2%, P = 0.018). No association was found between aberrant DNA methylation and selected factors including sex, age, tobacco smoking, alcohol consumption and green tea consumption. After adjusting for potential confounding variables, variant allele of MTHFR a1298c was found to be associated with methylation of the Dact1 gene. Compared with wild type CC, the odds ratio was 4.33 (95% CI: 1.06-10.59) for AC and 4.95 (95% CI: 1.18-12.74) for AA. The N stage in TNM staging and the occurrence of lymph node metastasis were associated with an MTHFR 1298 AA+AC genotype (P<0.05). Conclusion: MTHFR 1298 AC and AA genotypes might help maintain a normal methylation status of the Dact1 gene, aberrant CpG island methylation of which is closely related to the genesis and progression of transitional cell carcinoma.

• Biomolecules & Therapeutics
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• 제30권5호
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• pp.435-446
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• 2022

The present study evaluated the anti-cancer activity of histone deacetylase (HDAC)-inhibiting CKD-581 in multiple myeloma (MM) and its pharmacological mechanisms. CKD-581 potently inhibited a broad spectrum of HDAC isozymes. It concentration-dependently inhibited proliferation of hematologic cancer cells including MM (MM.1S and RPMI8226) and T cell lymphoma (HH and MJ). It increased the expression of the dishevelled binding antagonist of β-catenin 3 (DACT3) in T cell lymphoma and MM cells, and decreased the expression of c-Myc and β-catenin in MM cells. Additionally, it enhanced phosphorylated p53, p21, cleaved caspase-3 and the subG1 population, and reversely, downregulated cyclin D1, CDK4 and the anti-apoptotic BCL-2 family. Finally, administration of CKD-581 exerted a significant anti-cancer activity in MM.1S-implanted xenografts. Overall, CKD-581 shows anticancer activity via inhibition of the Wnt/β-catenin signaling pathway in hematologic malignancies. This finding is evidence of the therapeutic potential and rationale of CKD-581 for treatment of MM.

• Journal of Ginseng Research
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• 제23권2호
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• pp.93-98
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• 1999

홍삼 exoact를 첨가한 액상 제품에서의 침전 생성을 감소시키기 위하여 홍삼 exoact에 ${\beta}-amylase$와 transglucosidase를 처리한 후 품질 특성의 변화를 조사하였다. 이소말토올리고당인 isomatose 가 $5.25\%$ 생성되었고 glucose의 함량이 증가하였으며, ginsenoside는 $Rb_1$과 $Rb_2$가 감소하였고 Rd의 함량이 증가하였다. 효소처리 한 홍삼 exDact는 무처리구에 비하여 Bifdus속균과, Lactobacizlus속균과 같은 장내유용세균에 대한 증식효과가 있었으며 Bifdus속균은 Lactobacizlus속균보다 대체로 흥삼 extract 첨가에 의한 생육도가 낮았다. 효소처리한 홍삼 extract는 단맛과 신맛이 증가하였으나 홍삼 extrac의 좋지 않은 맛으로 평가되는 쓴맛과 떫은맛이 감소하여 향미가 개선되었다. 효소처리 한 홍삼 extract를 첨가하여 $pH\;3.0\~4.5$의 음료를 제조한 다음 $40^{\circ}C$에서 1개월간 저장하였을 때 무처리구에 비하여 침전량이 현저히 감소하였으나 pH 3.0에서는 효소처리 한 홍삼 extract첨가구에서 $17.7\%$, 무처리구에서 $30.3\%$의 침전이 형성되었다. 따라서 인삼 음료의 침전을 감소시키기 위하여는 홍삼 exoact에 P-amylase와 transglucosidase를 처리하고 동시에 음료의 pH를 3.5 이상으로 유지하는 것이 요구되었다.