• Title/Summary/Keyword: D605E

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Detection of Human Cytomegalovirus UL97 D605E Mutation in Korean Stem Cell Transplantation Recipients and Donors

  • Lee, Gyu-Cheol;Choi, Su-Mi;Lee, Chan Hee;Lee, Dong-Gun;Choi, Jung-Hyun;Yoo, Jin-Hong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.8
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    • pp.1154-1158
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    • 2013
  • Ganciclovir resistance of human cytomegalovirus is associated with mutations in the viral UL97 gene and poses severe problems for immunocompromised patients. In this study, PCR-based restriction fragment length polymorphism and sequencing analyses detected the UL97 D605E mutation in all five clinical isolates from patients with ganciclovir-resistant human cytomegalovirus infection during prolonged ganciclovir therapy, whereas the M460V mutation was only present in 1 of 5 isolates. On the other hand, the detection rates of the D605E mutation in the stored available DNA samples from the donor and allogeneic stem cell transplantation recipients were 66.7% and 93.7%, respectively, suggesting that the presence of D605E mutation was not associated with the ganciclovir exposure. Although the D605E mutation may not be related to ganciclovir resistance, we suggest that this mutation could be an important molecular marker of human cytomegalovirus evolution in East Asian countries. Moreover, the restriction fragment length polymorphism method using the restriction enzyme HaeIII, which is generally used to detect the UL97 A591V mutation, could also detect the D605E mutation and may therefore be a useful tool for future research on the investigation of UL97 gene mutations.

Comparative analysis of men's slim pants patterns - Using a 3D CLO virtual garment system - (남성 슬림 팬츠 패턴 비교 분석 - 3D CLO 가상착의 시스템을 활용하여 -)

  • Kim, Kyung-A;Hong, Eun-Hee;Uh, Mi-Kyung
    • The Research Journal of the Costume Culture
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    • v.22 no.4
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    • pp.605-618
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    • 2014
  • This study aims to compare the ease of men's slim pants patterns, and to analyze the fit and appearance through the 3D virtual garment system. The study selected four educational materials and one industrial pattern of slim pants for a total of five items. The CLO 3D Modelist program was utilized to carry out the appearance evaluation through virtual wearing and opacity, and a comparison was performed regarding the clothing pressure when the virtual model was standing and walking. The results of our comparison of the patternmaking for slim pants showed that pattern C pants had the greatest ease on the waist circumference, while B pants showed no ease. The C and E pants also had the most ease on the hip measurement. In the appearance evaluation, A pants received the most favorable results, followed by D, E, B, and C, in descending order. The clothing pressure appeared to be mainly red on the waist, crotch, and hem when standing in all pants, so the clothing pressure was high. While walking, the stress appeared to be different for the left and right parts of the body, and the most significant difference was observed on the thigh area.

Nutrient Requirements of Exercising Swamp Buffalo, Bubalus bubalis, from Materials Balance and In Vivo Body Composition by the Body Density Method. I. Aspects of Energy and Protein Metabolism in Working Cows

  • Mahardika, IG.;Sastradipradja, D.;Sutardi, T.;Sumadi, IK.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.5
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    • pp.605-612
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    • 2000
  • Four young swamp buffalo cows of similar age ranging in weight between 280 to 380 kg and trained to do physical work were used in a study to determine energy and protein requirements for draught using a $4{\times}4$ Latin square designed experiment. The experiment consisted of field trials employing 4 levels of work load, e.g. no work as control, and loads amounting 450 to 500 Newton (N) pulled continuously for 1, 2 and 3 h daily for 14 consecutive days. Cows were fed king grass (Penisetum purpuroides) ad libitum and were subjected to materials balance trials. Body composition was estimated in vivo by the body density method and daily energy expenditure (EE) was calculated from ME minus retained energy (RE). The results show that EE while not working ($EE_{resting}$) was $0.42kgW^{0.75}MJ/d$ and maintenance ME ($ME_m$) was $0.37kgW^{0.75}MJ/d$. ME requirement increased to 1.65 times maintenance for the work of 3 hours. The energy expended for doing exercise ($E_{exercise}$) was 9.56, 20.0 and 25.86 MJ/cow for treatments 1, 2 and 3 II, respectively. Fat retention was absent in all groups of working cows, but protein retention was only negative for cows undertaking 3 h work. The relationship between $E_{exercise}$ (MJ), work load (F, kN), work duration (t, h) and body mass (W, kg) was found to be: $E_{exercise}=(0.003F^{1.43}t^{0.93})/W^{0.09}MJ$. The maintenance requirement for digestible protein was $2.51kgW^{0.75}g/d$, whereas digestible protein for growth ($DP_{growth}$) and for work ($DP_{work}$) followed the equations: $DP_{growth}=[(258+1.25W^{0.75}){\Delta}Wkg/d]g$ and $DP_{work}=[12.59e^{0.95t}]g$, respectively The coefficients a, b and c for the calculation of $E_{exercise}$ components according to the Lawrence equation were found to be 2.56 J/kgW.m, 5.2 J/kg load carried.m and 0.29, respectively, thus efficiencies to convert ME into work were 0, 16.09, 27.3 and 32.44% for control, 1, 2 and 3 h/d work, respectively. ME and DP requirements for a 250 to 400 kg working buffalo cow allowing to growth up to 0.5 kg/d are presented.

An Efficient Path Expression Join Algorithm Using XML Structure Context (XML 구조 문맥을 사용한 효율적인 경로 표현식 조인 알고리즘)

  • Kim, Hak-Soo;Shin, Young-Jae;Hwang, Jin-Ho;Lee, Seung-Mi;Son, Jin-Hyun
    • The KIPS Transactions:PartD
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    • v.14D no.6
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    • pp.605-614
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    • 2007
  • As a standard query language to search XML data, XQuery and XPath were proposed by W3C. By widely using XQuery and XPath languages, recent researches focus on the development of query processing algorithm and data structure for efficiently processing XML query with the enormous XML database system. Recently, when processing XML path expressions, the concept of the structural join which may determine the structural relationship between XML elements, e.g., ancestor-descendant or parent-child, has been one of the dominant XPath processing mechanisms. However, structural joins which frequently occur in XPath query processing require high cost. In this paper, we propose a new structural join algorithm, called SISJ, based on our structured index, called SI, in order to process XPath queries efficiently. Experimental results show that our algorithm performs marginally better than previous ones. However, in the case of high recursive documents, it performed more than 30% by the pruning feature of the proposed method.

The Effects of Addition of Apple Pomace to Rice Straw Silage on Feed Intake and Digestibility of Korean Native Goats (사과박 첨가 볏짚 사일리지가 한국 재래산양의 사료 섭취량과 소화율에 미치는 영향)

  • 조익환;황보순;전하준;안종호;이주삼;한태호
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.2
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    • pp.107-114
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    • 2002
  • Rice straw silage added with apple pomace in different ratios were mixed with commercial concentrates and fed to Korean native goats in comparison to the corn silage mixed with the same content of commercial concentrates in whole diet as that of apple pomace added rice straw silage (rice straw : apple pomace = 100:0, 80:20, 60:40, 40:60). Dry matter intake, digestibilities of nutrients and nitrogen retention were investigated for the possibilities of application of agricultural by-products for the diets of Korean native goats. Crude protein contents of rice straw silage added with apple pomace were 6.3~7.3% and the contents of ADF, NDF and crude ash were highest in 100% rice straw mixed ratio (A) as 39.4, 61.6 and 8.9% respectively. Those were lower in corn silage (E) as 30.3, 53.4 and 4.9% respectively, however NSC content of corn silage was highest among the experimental treatments as 31.4%. Daily dry matter intakes per head and also per metabolic basal weight (DM g/kg of $BW^{0.75}$ were significantly (p<0.05) higher in D of the highest mixed ratio of apple pomace (605.3, 69.5g) than those of corn silage (E: 394.0, 46.8g). Daily live weight changes were significantly (p<0.05) higher in 40% (C: 16.7g) and 60% (D: 22.9g) apple pomace mixed ratios than 0% (A: 0.17g) and 20% (B: 4.3g) apple pomace mixed ratios. Digestibilities of dry matter and organic matter were higher in D and E than in A and B and those of ADF and NDF were higher in D as 50.2 and 57.4% respectively than the digestibilities in A, B and E. Nitrogen retention (g, %) was highest in D of the highest mixed apple pomace ratio (1.4g, 20.4%) however lowest in A (-0.3g, -7.75%).

Numerical simulation of compressive to tensile load conversion for determining the tensile strength of ultra-high performance concrete

  • Haeri, Hadi;Mirshekari, Nader;Sarfarazi, Vahab;Marji, Mohammad Fatehi
    • Smart Structures and Systems
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    • v.26 no.5
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    • pp.605-617
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    • 2020
  • In this study, the experimental tests for the direct tensile strength measurement of Ultra-High Performance Concrete (UHPC) were numerically modeled by using the discrete element method (circle type element) and Finite Element Method (FEM). The experimental tests used for the laboratory tensile strength measurement is the Compressive-to-Tensile Load Conversion (CTLC) device. In this paper, the failure process including the cracks initiation, propagation and coalescence studied and then the direct tensile strength of the UHPC specimens measured by the novel apparatus i.e., CTLC device. For this purpose, the UHPC member (each containing a central hole) prepared, and situated in the CTLC device which in turn placed in the universal testing machine. The direct tensile strength of the member is measured due to the direct tensile stress which is applied to this specimen by the CTLC device. This novel device transferring the applied compressive load to that of the tensile during the testing process. The UHPC beam specimen of size 150 × 60 × 190 mm and internal hole of 75 × 60 mm was used in this study. The rate of the applied compressive load to CTLC device through the universal testing machine was 0.02 MPa/s. The direct tensile strength of UHPC was found using a new formula based on the present analyses. The numerical simulation given in this study gives the tensile strength and failure behavior of the UHPC very close to those obtained experimentally by the CTLC device implemented in the universal testing machine. The percent variation between experimental results and numerical results was found as nearly 2%. PFC2D simulations of the direct tensile strength measuring specimen and ABAQUS simulation of the tested CTLC specimens both demonstrate the validity and capability of the proposed testing procedure for the direct tensile strength measurement of UHPC specimens.

Performance analysis of Savonius Rotor for Wave Energy Conversion using CFD

  • Zullah, Mohammed Aisd;Choi, Young-Do;Kim, Kyu-Han;Lee, Young-Ho
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.600-605
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    • 2009
  • A general purpose viscous flow solver Ansys CFX is used to study a Savonius type wave energy converter in a 3D numerical viscous wave tank. This paper presents the results of a computational fluid dynamics (CFD) analysis of the effect of blade configuration on the performance of 3 bladed Savonius rotors for wave energy extraction. A piston-type wave generator was incorporated in the computational domain to generate the desired incident waves. A complete OWC system with a 3-bladed Savonius rotor was modeled in a three dimensional numerical wave tank and the hydrodynamic conversion efficiency was estimated. The flow over the rotors is assumed to be two-dimensional (2D), viscous, turbulent and unsteady. The CFX code is used with a solver of the coupled conservation equations of mass, momentum and energy, with an implicit time scheme and with the adoption of the hexahedral mesh and the moving mesh techniques in areas of moving surfaces. Turbulence is modeled with the k.e model. Simulations were carried out simultaneously for the rotor angle and the helical twist. The results indicate that the developed models are suitable to analyze the water flows both in the chamber and in the turbine. For the turbine, the numerical results of torque were compared for all the cases.

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A Study on the Effect of Virtual Reality Intervention on Cognitive Function in Individuals With Stroke Through Meta-analysis (메타분석을 통한 뇌졸중 환자의 인지기능에 대한 가상현실 중재 효과 연구)

  • Kwon, Jae Sung
    • Therapeutic Science for Rehabilitation
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    • v.10 no.3
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    • pp.7-22
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    • 2021
  • Objective : The purpose of this study was to verify the effect of virtual reality interventions (VRIs) on cognitive function in individuals with stroke through a systematic literature review and meta-analysis. Methods : We reviewed randomized controlled trials (RCTs) the last 10 years using academic databases. PubMed, MEDLINE, and CINAHL were used for international studies, and DBpia, KISS, Kyoboscholar, and e-article were used for Korean studies. For the quantitative meta-analysis, subgroups of outcomes were classified into general cognitive function (G-CF), attention and memory (A&M), and executive function (EF). Results : Nine RCTs were analyzed. The total number of participants was 271 (140 in the experimental group). The effect size (Cohen's d) was estimated using a random effects model. The effect sizes of the outcome subgroups of were as follows: small to medium for G-CF (d=0.422; 95% CI: 0.101~0.742; p=0.010), small for A&M (d=0.249; 95% CI: -0.107~0.605; p=0.170), and medium for EF (d=0.666; 95% CI: 0.136~1.195; p=0.014). Conclusion : Considering the various stimuli provided by the virtual environment and the results from available research, virtual reality should be applied to interventions for integrated cognitive functions. In addition, it would be appropriate to be used as an additional intervention to traditional cognitive rehabilitation for stroke.

Process Characteristics for $YB_{2}Cu_{3}O_{7-d}$ Films Fabricated by Single Target Sputter and Surface Modification Technique

  • Lee, Eue-Jae
    • Korean Journal of Materials Research
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    • v.5 no.5
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    • pp.598-605
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    • 1995
  • Thin films of $YB_{2}Cu_{3}O_{7-d}$ were prepared on various substrated of MgO(100), $SrTiO_{3}$, and $LaAlO_{3}$ by using off-axis magentron sputtering methods and annealing in-situ. The prarameters of film fabrication processes had been optimized through a "follow the lcoal maxima" strategy to yield good quality films in therms of the critical temperature $T_{c}$ and the critical current density $J_{c}$. Optimizedproecsses employing a plane magndtron and an cylindrical magnetron yielded $T_{c}$>90K along with $J_{c}$$10^{6}$A/$\textrm{cm}^2$ at 77K and > 2${\times}$$10^{7}$A/$\textrm{cm}^2$ at 5K. The sampels, however, showed degradationinthe properties, after chemical etching for fabrication of microbridges with the line width of 2-10 mocrons. In particular, the value of $T_{c}$ for the microbridges of 2microns was as small as 80%. The degradation was strongly dependent on the line width through a formula : $T_{c}$(e)=$T_{c}$)b) [1-a exp(-1000 bL)} where $T_{c}$(e) and $T_{c}$ (b) are the values of $T_{c}$ in the absolute scale measured after and before chemical etching, respectively and L is the line width in mm. By utilizing a best fitting technique, the proper constant values of a and to b were found as exp(-1.2) and 0.22, respectively. This formula was very useful in estimatiing the upper limit of the device operationtemperature.

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Miniaturization of Circular Microstrip Antenna Using the Perturbation Effect (Perturbation 효과를 이용한 원형 마이크로스트립 안테나의 소형화)

  • Ryu Mi-Ra;Hur Jung;Woo Jong-Myung
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.7 s.110
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    • pp.605-614
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    • 2006
  • In this paper, a 3D structure skimmer-shaped circular microstrip antenna is designed, the ends of whose both sides are made as DC(Depressed Carving part) applying perturbation effect to reduce the patch size of microstrip antenna operating at design frequency 1.575 GHz. The result shows its return loss, - 10 dB bandwidth, gain, - 3 dB beamwidth E, H-plane are -26.59 dB, 65 MHz(4.13 %), 4.66 dBd, $79^{\circ},\;87^{\circ}$ respectively. The diameter of the antenna is 85 mm, which is 12.4 % reduced compared to the size(97 mm) of general microstrip patch antenna. Therefore its area reduction is 23.2 %. Furthermore, a linear and circular polarized baseball-shaped circular microstrip antenna is designed to minimize the patch size of the antenna. This structure of antenna operating at the design frequency 1.575 GHz is applied with the optimum RC(Raised Carving part) & DC ratio and an asymptotic line angle. In case of linear polarized baseball-shaped circular microstrip antenna, the patch size of the antenna is 74 mm, which is 41.8 % area reduction compared to general microstrip patch antenna. In case of circular polarized baseball-shaped circular microstrip antenna, the diameter of patch is 82 mm, which is 28.5 % area reduction compare to general microstrip patch antenna linear polarized. We have verified that the perturbation effect can be applied to minimize the circular microstrip antenna.