• Journal of Microbiology and Biotechnology
• /
• 제22권7호
• /
• pp.960-966
• /
• 2012

The diversity and composition of yeast populations may greatly impact wine quality. This study investigated the yeast microbiota in two different types of wine fermentations: direct inoculation of a commercial starter versus pied de cuve method at an industrial scale. The pied de cuve fermentation entailed growth of the commercial inoculum used in the direct inoculation fermentation for further inoculation of additional fermentations. Yeast isolates were collected from different stages of wine fermentation and identified to the species level using Wallersterin Laboratory nutrient (WLN) agar followed by analysis of the 26S rDNA D1/D2 domain. Genetic characteristics of the Saccharomyces cerevisiae strains were assessed by a rapid PCR-based method, relying on the amplification of interdelta sequences. A total of 412 yeast colonies were obtained from all fermentations and eight different WL morphotypes were observed. Non-Saccharomyces yeast mainly appeared in the grape must and at the early stages of wine fermentation. S. cerevisiae was the dominant yeast species using both fermentation techniques. Seven distinguishing interdelta sequence patterns were found among S. cerevisiae strains, and the inoculated commercial starter, AWRI 796, dominated all stages in both direct inoculation and pied de cuve fermentations. This study revealed that S. cerevisiae was the dominant species and an inoculated starter could dominate fermentations with the pied de cuve method under controlled conditions.

• 한국해양바이오학회지
• /
• 제1권2호
• /
• pp.119-125
• /
• 2006

어류의 insulin-like growth factor-I (IGF-I)의 생화학적 작용기작을 연구하기 위하여 한국산 조피볼락의 IGF-I cDNA 유전자 cloning을 행하였다. 완전한 cDNA 유전자 염기서열은 PCR과 RACE 방법을 통하여 얻어진 DNA로부터 결과를 얻을수 있었다. 결정된 IGF-I의 염기서열은 flounder, chinook salmon, human IGF-I의 염기서열과 비교한 결과 각각 93.6%, 90.7%, 85.4%의 높은 상동성을 보였다. 생화학적으로 활성이 있는 재조합 IGF-I을 얻기 위하여 IGF-I의 B-C-A-D domain 부분을 PCR로 얻은 뒤 E. coli BL21(DE3)에 넣어 overexpression 시켰다. Ni-NTA colummn을 사용하여 순수한 재조합 단백질을 정제할수 있었다. 정제된 단백질은 SDS-PAGE 상에서 7 kDa의 단일 band를 보여 주었으며 [$^3H$]-thymidine 결합정도를 측정하는 방법으로 활성을 가지고 있음을 확인할수 있었다.

• 한국소음진동공학회논문집
• /
• 제18권11호
• /
• pp.1157-1169
• /
• 2008

This paper presents spectral element formulation which approximates Lamb wave propagation by PZT transducers bonded on a thin plate. A two layer beam model under 2-D plane strain condition is introduced to simulate high-frequency dynamic responses induced by a piezoelectric (PZT) layer rigidly bonded on a base plate. Mindlin-Herrmann and Timoshenko beam theories are employed to represent the first symmetric and anti-symmetric Lamb wave modes on a base plate, respectively. The Euler-Bernoulli beam theory and 1-D linear piezoelectricity are used to model the electro-mechanical behavior of a PZT layer. The equations of motions of a two layer beam model are derived through Hamilton's principle. The necessary boundary conditions associated with the electro-mechanical properties of a PZT layer are formulated in the context of dual functions of a PZT layer as an actuator and a sensor. General spectral shape functions of response field and the associated boundary conditions are obtained through equations of motions converted into frequency domain. Detailed spectrum element formulation for composing the dynamic stiffness matrix of a two layer beam model is presented as well. The validity of the proposed spectral element is demonstrated through numerical examples.

• 지구물리와물리탐사
• /
• 제14권1호
• /
• pp.98-104
• /
• 2011

탐사 지구물리학에서 수치 모사는 지하매질에서의 탄성파 전파 현상을 이해하는데 중요한 통찰력을 제공한다. 탄성파 모사는 음향파 근사에 의한 수치 모사보다 계산시간이 많이 소요되지만 전단응력 성분을 포함하여 보다 현실적인 파동의 모사를 가능하게 한다. 그러므로 탄성파 모사는 탄성체의 반응을 탐사하는데 적합하다고 할 수 있다. 계산 시간이 길다는 단점을 극복하기 위해 본 논문에서는 그래픽 프로세서(GPU)를 이용하여 탄성파 수치 모사 시간을 단축하고자 하였다. GPU는 많은 수의 프로세서와 광대역 메모리를 갖고 있기 때문에 병렬화된 계산 아카텍쳐에서 사용할 수 있는 장점이 있다. 본 연구에서 사용한 GPU 하드웨어는 NVIDIA Tesla C1060으로 240개의 프로세서로 구성되어 있으며 102 GB/s의 메모리 대역폭을 갖고 있다. NVIDIA에서 개발된 병렬계산 아카텍쳐인 CUDA를 사용할 수 있음에도 불구하고 계산효율을 상당히 향상시키기 위해서는 GPU 장치의 여러 가지 다양한 메모리의 사용과 계산 순서를 최적화해야만 한다. 본 연구에서는 GPU 시스템에서 시간영역 유한차분법을 이용하여 2차원과 3차원 탄성과 전파를 수치 모사하였다. 파동전파 모사에 가장 널리 사용되는 유한차분법 중의 하나인 엇갈린 격자기법을 채택하였다. 엇갈린 격자법은 지구물리학 분야에서 수치 모델링을 위해 사용하기에 충분한 정확도를 갖고 있는 것으로 알려져 있다. 본 논문에서 제안한 모델링기법은 자료 접근 시간을 단축하기 위해 GPU 장치를 메모리 사용을 최적화하여 가능한 더 빠른 메모리를 사용한다. 이점이 GPU를 이용한 계산의 핵심 요소이다. 하나의 GPU 장치를 사용하고 메모리 사용을 최적화함으로써 단일 CPU를 이용할 경우보다 2차원 모사에서는 14배 이상, 3차원에서는 6배 이상 계산시간을 단축할 수 있었다. 세 개의 GPU를 사용한 경우에는 3차원 모사에서 계산효율을 10배 향상시킬 수 있었다.

• BMB Reports
• /
• 제44권10호
• /
• pp.653-658
• /
• 2011

Sclerotinia sclerotiorum fungus has three endoxylanases induced by wheat bran. In the first part, a partial xylanase sequence gene (90 bp) was isolated by PCR corresponding to catalytic domains (${\beta}5$ and ${\beta}6$ strands of this protein). The high homology of this sequence with xylanase of Botryotinia fuckeliana has permitted in the second part to amplify the XYN1 gene. Sequence analysis of DNA and cDNA revealed an ORF of 746 bp interrupted by a 65 bp intron, thus encoding a predicted protein of 226 amino acids. The mature enzyme (20.06 kDa), is coded by 188 amino acid (pI 9.26). XYN1 belongs to G/11 glycosyl hydrolases family with a conserved catalytic domain containing $E_{86}$ and $E_{178}$ residues. Bioinformatics analysis revealed that there was no Asn-X-Ser/Thr motif required for N-linked glycosylation in the deduced sequence however, five O-glycosylation sites could intervene in the different folding of xylanses isoforms and in their secretary pathway.

• 한국생산제조학회지
• /
• 제23권1호
• /
• pp.75-82
• /
• 2014

For accurate gain tuning of the lab-manufactured six-axis articulated robot RS2 with less noise, in this study, a program routine using dynamic signal analyzer, which is a realization of a controller design algorithm in the frequency domain, is programmed using LabVIEW$^{(R)}$. The contribution of this paper is the proposal of a simulation technique based on SolidWorks$^{(R)}$ and LabVIEW$^{(R)}$ for the gain tuning of a six-axis articulated robot. To realize the simulation, the LabVIEW$^{(R)}$ program used for experimental gain tuning is incorporated in to SolidWorks$^{(R)}$. A comparison shows that the results of simulation-based gain tuning and experimental gain tuning are almost the same within a 5% error bound. On the basis of the comparison, it can be suggested that the simulation-based technique for gain tuning can be applied instead of experimental gain tuning to a six-axis articulated robot by interlocking SolidWorks$^{(R)}$ and LabVIEW$^{(R)}$.

• Journal of Astronomy and Space Sciences
• /
• 제23권3호
• /
• pp.199-208
• /
• 2006

The terrestrial impact cratering record recently has been examined in the time domain by Chang & Moon (2005). It was found that the ${\sim}26$ Myr periodicity in the impact cratering rate exists over the last ${\sim}250$ Myrs. Such a periodicity can be found regardless of the lower limit of the diameter up to D ${\sim}35km$. It immediately called pros and cons. The aim of this paper is two-fold: (1) to test if reported periodicities can be obtained with an independent method, (2) to see, as attempted earlier, if the phase is modulated. To achieve these goals we employ the time/frequency analysis and for the first time apply this method to the terrestrial impact cratering records. We have confirmed that without exceptions noticeable peaks appear around ${\sim}25$ Myr, corresponding to a frequency of ${\sim}0.04(Myr)^{-1}$. We also find periodicities in the data base including small impact craters, which are longer. Though the time/frequency analysis allows us to observe directly phase variations, we cannot find any indications of such changes. Instead, modes display slow variations of power in time. The time/frequency analysis shows a nonstationary behavior of the modes. The power can grow from just above the noise level and then decrease back to its initial level in a time of order of 10 Myrs.

• 한국양식학회지
• /
• 제12권2호
• /
• pp.91-100
• /
• 1999

The complete nucleotide sequence of olive flounder (Paralichthys olivaceus) hsp70-related rDNA was determined by RT- and RACE-PCR methods. A full-length of hsp70-related cDNA has an open reading frame of 1.95 kb encoding 650 amino acids with a calculated molecular weight of 71.1 kD. A corresponding hsp70-related protein contains a number of conserved elements including an ATP-binding domain, a nuclear localization signal and the carboxyl terminal motif, EEVD, which may have a role in chaperone function. Comparison of nucleotide and predicted amino acid sequence between olive flounder hsp70-related gene and hsp/hsc70 genes of other species revealed a high similarity with the cognate form of these genes. These results indicated that we recovered likely to be a olive flounder cognate hsc70 gene.

• Journal of Magnetics
• /
• 제8권1호
• /
• pp.24-31
• /
• 2003

The discoveries of antiferromagnetic coupling in Fe/Cr multilayers by Grunberg, the Giant Magneto Resistance by Fert and Grunberg and a large tunneling magnetoresistance at room temperature by Moodera have triggered enormous research on magnetic thin films and magnetoelectronic devices. Large opportunities are especially opened by the spin dependent tunneling resistance, where a strong dependence of the tunneling current on an external magnetic field can be found. We will briefly address important basic properties of these junctions like thermal, magnetic and dielectric stability and discuss scaling issues down to junction sizes below 0.01 $\mu\textrm{m}$$^2$with respect to single domain behavior, switching properties and edge coupling effects. The second part will give an overview on applications beyond the use of the tunneling elements as storage cells in MRAMs. This concerns mainly field programmable logic circuits, where we demonstrate the clocked operation of a programmed AND gate. The second 'unconventional' feature is the use as sensing elements in DNA or protein biochips, where molecules marked magnetically with commercial beads can be detected via the dipole stray field in a highly sensitive and relatively simple way.

• 한국군사과학기술학회지
• /
• 제5권2호
• /
• pp.169-184
• /
• 2002

In order to extract bearing information from the directional sensors of DIFAR(directional frequency analysis and recording) that is a kind of passive sonobuoy, the cardioid beamforming algorithm applicable to DIFAR system was studied in the frequency domain. the algorithm uses narrow-band signals propagated though the media from the acoustic sources such as ship machineries. The proposed algorithm is expected to give signal to noise ratio of 6dB when it uses the maximum response axis(MRA) among the Cardioid beams. The estimated bearings agree very well with those from GPS data. Assuming the bearings from GPS data to be real values, the estimation errors are analyzed statistically. The histogram of estimation errors in each frequency have Gaussian shape, the mean and standard deviation dropping in the ranges -1.1~$6.7^{\circ}$ and 13.3~$43.6^{\circ}$, respectively. Estimation errors are caused by SMR degradation due to propagation loss between the source and receiver, daily fluctuating geo-magnetic fields, and non-stationary background noises. If multiple DIFAR systems are employed, in addition to bearing, range information could be estimated and finally localization or tracking of a target is possible.