• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.905-912
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• 2013

A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03-$0093^T$, ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03-$0093^T$ (= NBRC $101693^T$ =UICC $Y-335^T$ = BTCC-$Y25^T$). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needle-shaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-$0093^T$, differed from M. reukaufii NBRC $1679^T$ by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-$0093^T$, ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of $44.05{\pm}0.25%$, whereas that of M. reukaufii NBRC $1679^T$ was 41.3%. The low value of DNA-DNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC $1679^T$ strongly supported that these strains represent a distinct species.

• Journal of Broadcast Engineering
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• v.18 no.4
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• pp.543-549
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• 2013

Motion artifacts of central and autonomic nervous system signals degrades the performance of the bio-signal based human factor analysis. Firstly, we propose a defining method of motion artifact section by analyzing successive image frames. Motion artifact section is defined when the amount of motion is greater than the pre-defined threshold. In here, the amount of motion is estimated by first derivation of image frames at temporal domain. Secondly, we propose another defining method of motion artifact section through designing 2D Gaussian probability density function model by analyzing feature vectors of one cycle of signal such as length and amplitude. The defined motion artifact sections are interpolated on the basis of 1D Gaussian function. At result of applying the method into photoplethysmography signal, we confirmed that the calculated heartbeat rate from the restored photoplethysmography came up to the one from electrocardiography. Also, we found that the video based method generated relatively more false acceptance of motion artifact section and the probability density function based method generated relatively more false rejection of motion artifact section.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.2
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• pp.187-196
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• 2013

A numerical model was developed to predict the density wave oscillation (DWO) in the furnace wall tubes of a fossil-fired once-through boiler. The transient flow fields in the tubes were obtained using a 1D finite volume method in the time domain. A header model was also implemented to simulate the parallel tube connection of the wall tubes. The inlet and outlet mass flow variation in one of the parallel tubes was examined after a heat perturbation to find the DWO. After successful verification with experimental results reported in literature, the developed model was applied to the wall tubes of a 700-MW boiler furnace. In contrast to the simulation of Takitani's experiment, in which the unstable power thresholds tended to rise in the reduced bypass channel flow, no remarkable changes were observed in the power thresholds in the parallel channel modeling of the wall tubes of the boiler furnace.

• The Journal of the Acoustical Society of Korea
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• v.32 no.2
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• pp.116-123
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• 2013

This paper represents generation of time-varying underwater acoustic channels by performing scattering simulation with time-varying ocean surface and Kirchhoff approximation. In order to estimate the time-varying ocean surface, 1D Pierson-Moskowitz ocean power spectrum and Gaussian correlation function were used. The computed scattering coefficients are applied to the amplitudes of each impulse of BELLHOP simulation result. The scattering coefficients are then compared with measured doppler spectral density of signal components which were scattered from ocean surface and the correlation time used in the Gaussian correlation function was estimated by the comparison. Finally, bit-error-rate and channel correlation simulations were performed with the generated time-varying channel based on passive time-reversal communication scenario.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1163-1175
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• 2021

In autonomous driving, road markings are an essential element for object tracking, path planning and they are able to provide important information for localization. This paper presents an approach to update and measure road surface markers with HD maps as well as matching using inverse perspective mapping. The IPM removes perspective effects from the vehicle's front camera image and remaps them to the 2D domain to create a bird-view region to fit with HD map regions. In addition, letters and arrows such as stop lines, crosswalks, dotted lines, and straight lines are recognized and compared to objects on the HD map to determine whether they are updated. The localization of a newly installed object can be obtained by referring to the measurement value of the surrounding object on the HD map. Therefore, we are able to obtain high accuracy update results with very low computational costs and low-cost cameras and GNSS/INS sensors alone.

• Geomechanics and Engineering
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• v.36 no.4
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• pp.367-380
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• 2024

Investigations has shown that the correct estimation of the effective amplification period is as important as the amplification value itself. It gets more important in 2D basins. This study presents a quantitative coefficient for consideration of the nonlinearity effect in terms of amplification value and the shift in its period which is missing or ineffectively considered in the previous studies. To attain this goal, by the application of a time domain fully nonlinear method, the deviation of the more common equivalent linear results from the basin nonlinear behavior under strong ground motions is investigated quantitatively. Also, despite the increase in the damping ratio, the possibility of the increase in the amplification due to the increase in motion strength is shown. To make the results useful in engineering practice, by introducing nonlinearity ratio, the effect of the nonlinearity is quantitatively estimated for two soft and stiff clayey basins with three different depths under a set of motions scaled to two target spectrum. Results show that at the 100 m depth soft clayey basin, while the nonlinearity ratio shows a 35% deviation at the basin edge part under DD1 motion level, its effect moves to the central part with 20% effect under DD3 motion level. By the increase in depth to 150 m, the results show a decrease in the overall effect of the nonlinear behavior for both clay types. At this depth, the nonlinearity ratio gives a 30% and 17% difference on a limited distance from outcrop at the soft clayey basin under DD1 and DD3 motion levels, respectively. At the 30 m depth basins, the nonlinearity ratio shows up to 25% difference for different cases. The presented ratio would be introduced as nonlinearity coefficients for consideration of the nonlinearity effects in the codes. The presented quantitative margins will help the designer to have a better understanding of the amplification period change because of nonlinearity over 2D basin surface.

• Mycobiology
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• v.48 no.2
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• pp.122-132
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• 2020

Citric acid is a commercially valuable organic acid widely used in food, pharmaceutical, and beverage industries. In this study, 260 yeast strains were isolated from soil, bread, juices, and fruits wastes and preliminarily screened using bromocresol green agar plates for their ability to produce organic acids. Overall, 251 yeast isolates showed positive results, with yellow halos surrounding the colonies. Citric acid production by 20 promising isolates was evaluated using both free and immobilized cell techniques. Results showed that citric acid production by immobilized cells (30-40 g/L) was greater than that of freely suspended cells (8-19 g/L). Of the 20 isolates, two (KKU-L42 and KKU-L53) were selected for further analysis based on their citric acid production levels. Immobilized KKU-L42 cells had a higher citric acid production rate (62.5%), while immobilized KKU-L53 cells showed an ~52.2% increase in citric acid production compared with free cells. The two isolates were accurately identified by amplification and sequence analysis of the 26S rRNA gene D1/D2 domain, with GenBank-based sequence comparison confirming that isolates KKU-L42 and KKU-L53 were Candida tropicalis and Pichia kluyveri, respectively. Several factors, including fermentation period, pH, temperature, and carbon and nitrogen source, were optimized for enhanced production of citric acid by both isolates. Maximum production was achieved at fermentation period of 5 days at pH 5.0 with glucose as a carbon source by both isolates. The optimum incubation temperature for citric acid production by C. tropicalis was 32 ℃, with NH₄Cl the best nitrogen source, while maximum citric acid by P. kluyveri was observed at 27 ℃ with (NH₄)₂ SO₄ as the nitrogen source. Citric acid production was maintained for about four repeated batches over a period of 20 days. Our results suggest that apple and banana wastes are potential sources of novel yeast strains; C. tropicalis and P. kluyveri which could be used for commercial citric acid production.

• 한국지구물리탐사학회:학술대회논문집
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• 2003.11a
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• pp.244-251
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• 2003

A two-dimensional BEM code, $FRACOD^{2D}$, was applied to simulate fracture initiation and propagation processes in a rock pillar during an in situ heater test of a rock pillar planned at the $\"{A}sp\"{o}$ Underground Rock laboratory of SKB, in Southern Sweden. To take the advantage of conventional BEM for simulating fracturing processes, but without efforts for domain integral transformation, a hybrid approach is developed to simulate the fracturing processes in rock pillar under coupled thermo-mechanical loading. The code FRACOD was used for simulating the fracture initiation and propagation processes with its boundary tractions reflecting the effects of the initial and redistributed thermomechanical stresses in the domain of interest at multiple excavation and heating steps were produced by a special algorithm of stress inversion, based on resultant thermo-mechanical stress fields at each excavation and heat loading step by a FEM code without considering fracturing processes. This hybrid approach can take the advantages of both types of numerical methods and avoids their shortcomings for fracturing process simulation and domain effects, respectively. In this paper, we present the hybrid approach for the stress, displacements, and fracturing processes at sequential excavation and heating steps of the in situ heater test as a predictive modelling, the formulation of the fracturing models and the predictive results. Two sections of borehole depth, 0.5 m and 1.5 m below the tunnel floor are considered. The pillar area is modelled with the FRACOD and the stress field produced by excavation and heating is transferred with corresponding boundary stresses. From the modelling results, the degree of fracturing and damage are evaluated for 120 days of heating. Dominated shear fracturing in the vicinity of the central pillar was observed from the models at both sections, but spalled area appears to be limited. Based on the modelling results, a sensitivity study for the effect of pre-existing fractures in the vicinity of the holes is also conducted, and the initiation and evolution of EDZ around the deposition holes are investigated using this particular numerical technique.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.12 no.2
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• pp.217-227
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• 1992

The first and second-order reliability method(FORM and SORM) is presented using one dimensional finite difference and two dimensional finite element transport models. FORM and SORM can be used without any restrictive assumptions about the properties of the media, and the sensitivity information obtained as part of these analyses is used to identify the parameters which have major influence on the estimate of probability. The reliability analysis of transport in a one-dimensional domain is used to test the robustness of the reliability code and to evaluate the accuracy of the reliability method. A continuous source 2-D example with a concentration threshold limit state function is used to evaluate the influence of the parameters in the location of interest on the reliability solution.

• BMB Reports
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• v.43 no.1
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• pp.17-22
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• 2010

In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling.