• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.143-150
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• 1998

Many enzymes catalyze a primary reaction and/or secondary reaction. Dextransucrase usually synthesize dextran from sucrose as a primary reaction. The secondary reaction of dextransucrase is the transfer of glucose from sucrose to carbohydrate accepters. We have reacted dextransucrase from Leuconostoc mesenteroides B-742CB with sucrose and starches; granule or gelatinized starches, and Small or Potato starches. The yield of modified starch was ranged from 46% to 72%(s.d.<${pm}$5%) of theoretical depends on various reaction conditions. Modified products were more resistant against the hydrolysis of ${alpha}$-amylase, isoamylase, pullulanase and endo-dextranase than those of native starch. Based on the reactions from enzyme hydrolysis and methylation followed by acid hydrolysis modification of granule starch was more efficient than the modification of gelatinized starch. After modification of granule starch with dextransucrase, there produced a soluble modified starch. After modification the starch granules were fractionated to small size. The positions of glucose substitution of the modified products were determined by methylation followed by acid hydrolysis and analyzed by TLC. The products were modified by the addition of glucose to the position of C3, C4 and C6 free hydroxyl group of glucose residues in the starch.

• Journal of the Korea Concrete Institute
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• v.18 no.5 s.95
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• pp.621-630
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• 2006

Twelve beams made of lightweight high-strength concrete were tested to determine their diagonal cracking and ultimate shear capacities. A total of 12 beams without(4 beams) and with lightweight(8 beams) were tested in a stiff testing facility, and complete load-midspan deflection curves, including the maximum capacities portion, were obtained. The variables in the test program were concrete strength, which varied 35.4 MPa, 65.3 MPa; shear span-depth ratios a/d=1.5, 2.5, 3.5, 4.5; and tensile steel ratio between 0.57 and 2.3 percent. Also, we divided beam by diagonal tension crack and ultimate shearing strength to propose an equation. In addition, it analyzed comparison mutually applying existing proposal and guide. $V_{cr}$ was as result that AIK recommendations and Zsutty proposal decrease more than a/d=2.5, increased some in Mathey's proposal equation. $V_{cr,\exp}/V_{cr,cal}$ showed tendency of overestimation according to increase of tensile steel ratio and compressive strength of concrete. On the other hand, $V_{cr,\exp}/V_{cr,cal}$ is superior in conformability with an experiment result Zsutty's proposal among other equations. The proposal equation hew that expect $V_{cr}/V_u$, rationally about shearing strength. Therefore, shear strength an equation is considered to be utilized usefully evaluating capacity by change of the shear span depth ratio of lightweight concrete, tensile steel ratio, and compressive strength of the concrete in this research.

• Journal of the Korean Chemical Society
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• v.41 no.2
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• pp.98-104
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• 1997

A simple, accurate and reproducible capillary electrophoresis(CE) assay has been developed for the determination of berberine, cinnamic acid, and glycyrrhizin which are used in traditional Korean medicinal preparations. Separation of these compounds was performed in 20 mM phosphate buffer(pH 7.5) and acetonitrile(75:25, v/v) using a bare fused silica capillary($57 cm{\times}75 {\mu}m$ i.d.) at 25$^{\circ}C$. With the electric field of 350 V/cm, the time needed for the separation of berberine, cinnamic acid and glycyrrhizin was within 13 min. Calibration curves were linear for 1∼100 ${\mu}g/mL$ berberine, 0.3∼100 ${\mu}g/mL$ cinnamic acid and 2.5∼100 ${\mu}g/mL$ glycyrrhizin. The ranges of relative standard deviations(n=5) for those samples were between 0.96∼2.35%. The limits of detection(S/N=3) for berberine, cinnamic acid and glycyrrhizin were 0.5, 0.1 and 2.0 ${\mu}g/mL$, respectively. The numbers of theoretical plates were 181,000(berberine), 88,000(cinnamic acid) and 169,000(glycyrrhizin), while they were 3,100∼4,800 in HPLC.

• International Commerce and Information Review
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• v.14 no.2
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• pp.121-145
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• 2012

This research was performed to review the marketing PR(MPR) strategy to build the brand image of Chungnam tourism for the inducement of foreign tourist. Because each local governments are looking for the industries of new growth power from the tourism industry recently, we applied the BI strategy of Chungnam tourism with expended BLC model and GRID model after we analyzed the variety of PR situation and tasks to build the Chungnam tourism's brand image in the competitive domestic and foreign tourism industry. In the result of the study, we set up the limited brand image of Chungnam tourism newly; also we derived a specific action plan. First of all, they need every possible means for Brand Awareness PR, Issue PR, Brand Loyalty PR, etc. depend on brand life cycle with the Chungnam's tourism service products. Secondly, for the each tourism service products, it is requiring to repositioning from present position (B, C, D area) to A area by MPR strategy. Finally, many additional MPR strategies should put in to support for building the brand image for the 'Tourism Chungnam'. The strategic SNS public relations are required with considerations of items that are necessarily need to have the reasons and circumstances for the new foreign tourists, so they can have experiences for the Chungnam Tourism. For the existing foreign tourists, we should keep to build relationship in order to give them trusts and to feel sympathy.

• The Journal of the Acoustical Society of Korea
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• v.35 no.2
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• pp.92-101
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• 2016

There are many results in which acoustical conditions of a classroom play an important role for studying effects and academic achievement of students. However, there are very few guidelines or design proposals which could make appropriate acoustic environment when classrooms are built or renovated. The present study suggests various design proposals satisfying acoustic standards of classrooms based on theoretical calculation and acoustic field experiments. At first, minimum area of sound absorption was calculated which is required to satisfy the acoustic standard for domestic middle and high schools. Also, room acoustic measurements were carried out in order to investigate the acoustic performance of an existing classroom by changing interior finishing materials on ceiling and rear walls. As a result, it was revealed that reverberation time standard below 0.8 s can be acquired even if there is no sound absorption on ceiling which is a general practice executed in Korea. Specially, it was found that if partial area of ceiling would be treated as reflective with the ratio of sound absorption and reflection as 2:1, almost similar acoustic parameters of $C_{50}$, $D_{50}$, RASTI (Rapid Speech Transmission Index) and higher sound levels could be acquired in comparison with the case of entire sound absorption on ceiling.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.88-95
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• 2018

Maintainability indicates how easily a system can be restored to the normal state when a system failure occurs. Systems developed to have high maintainability can be competitive due to reduced maintenance time, workforce and resources. Quantification of the maintainability is possible in many ways, but only after prototype production or with historical data. As such, the graph theory and 3D model data have been used, but there are limitations in management efficiency and early use. To solve this problem, we studied the maintainability index of weapon systems using SysML-based modeling and simulation technique. A SysML structure diagram was generated to simultaneously model the system design and maintainability of system components by reflecting the maintainability attributes acquired from the system engineering tool. Then, a SysML parametric diagram was created to quantify the maintainability through simulation linked with MATLAB. As a result, an integrated model to account for system design and maintainability simultaneously has been presented. The model can be used from early design stages to identify components with low maintainability index. The design of such components can be changed to improve maintainability and thus to reduce the risks of cost overruns and time delays due to belated design changes.

• The Journal of the Acoustical Society of Korea
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• v.28 no.4
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• pp.318-327
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• 2009

In this paper, we propose a 3-D geometric localization method for near-field broadband source in shallow water environments. According to the waveguide invariant theory, slope of the interference pattern which is seen in a sensor spectrogram directly proportional to a range of the source. The relative ratio of the range between source and sensors was estimated by matching of two interference patterns in spectrogram. Then this ratio is applied to the Apollonius's circle which shows the locus of a source whose range ratio from two sensors is constant. Two Apollonius's circles from three sensors make the intersection point that means the horizontal range and the azimuth angle of the source. And this intersection point is constant with source depth. Therefore the source depth can be estimated using 3-D hyperboloid equation whose range difference from two sensors is constant. To evaluate a performance of the proposed localization algorithm, simulation is performed using acoustic propagation program and analysis of localization error is demonstrated. From simulation results, error estimate for range and depth is described within 50 m and 15 m respectively.

• Progress in Medical Physics
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• v.22 no.2
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• pp.61-66
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• 2011

In this study, we evaluated the effect of grid size on dose calculation accuracy using 2 head & neck and 2 prostate IMRT cases and based on this study's findings, we also evaluated the efficiency of a 2D diode array detector for IMRT quality assurance. Dose distributions of four IMRT plan data were calculated at four calculation grid sizes (1.25, 2.5, 5, and 10 mm) and the calculated dose distributions were compared with measured dose distributions using 2D diode array detector. Although there was no obvious difference in pass rate of gamma analysis with 3 mm/3% acceptance criteria for the others except 10 mm grid size, we found that the pass rates of 2.5, 5 and 10 mm grid size were decreased 5%, 20% and 31.53% respectively according to the application of the fine acceptance criteria, 3 mm/3%, 2 mm/2% and 1 mm/1%. The calculation time were about 11.5 min, 4.77 min, 2.95 min, and 11.5 min at 1.25, 2.5, 5, and 10 mm, respectively and as the grid size increased to double, the calculation time decreased about one-half. The grid size effect was observed more clearly in the high gradient area than the low gradient area. In conclusion, 2.5 mm grid size is considered acceptable for most IMRT plans but at least in the high gradient area, 1.25 mm grid size is required to accurately predict the dose distribution. These results are exactly same as the precious studies' results and theory. So we confirmed that 2D array diode detector was suitable for the IMRT QA.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.725-731
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• 2016

This study numerically investigates the thermal performance of a 2.0-kW butane-based combustion heating system for an electric vehicle under cold conditions. The system is used for cabin space heating and coolant-based battery thermal management. ANSYS CFX 17 software was used for parametric analysis. The mass flow rates of cold air and coolant were varied, and their effects were compared. The numerical results were validated with theoretical studies, which showed an error of 0.15%. As the outside air mass flow rates were increased to 0.005, 0.01, and 0.015 kg/s, the cabin supply air temperature decreased continuously while the coolant outlet temperature increased. When the coolant mass flow rates were increased to 0.005, 0.01 and 0.015 kg/s, the air temperature increased while the coolant outlet temperatures decreased. The optimal mass flow rates are discussed in a consideration of the requirements for high cabin heating capacity and efficient battery thermal management.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.103-103
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• 2015

유류유출사고와 같이 하천 수표면의 흐름에 따라 이동 확산하는 부유성 오염물질의 혼합해석을 위해 많은 연구자들은 입자추적모형을 사용한 혼합모의를 수행해왔다. 입자추적모형에서 오염물질의 혼합은 평균 유속 분포에 의한 결정론적인 이동과 난류유동에 의한 무작위적인 혼합으로 나타내며, 난류혼합에 의한 수평확산은 난류확산계수로 조절한다. 따라서 표면흐름에 의한 난류확산계수의 산정을 위해 많은 연구자들은 부유성 입자를 이용한 실내실험을 수행하여 수평확산계수를 산정했고(Engelund, 1969; Cederwall, 1971), 최근에는 GPS의 발전으로 인해 해양영역에서 GPS를 장착한 표면부자를 활용한 확산실험을 통해 수평확산계수를 산정한 바 있다(Kjellson and $D{\ddot{o}}{\ddot{o}}s$, 2012; Alpers 등, 2013). 하천수질오염사고의 약 43.5%가 유류유출에 의한 것이며(환경부, 2013), 이에 따라 표면흐름에 의한 오염물질 혼합해석이 필요하나, 하천에서 수평확산계수 산정을 위한 현장실험연구는 부족한 상황이다. 따라서 본 연구에서는 낙동강 본류에서 GPS부자를 이용한 입자추적실험을 수행하여 표면흐름에 의한 확산계수를 산정했다. GPS부자를 이용한 입자추적실험은 낙동강의 강정고령보 하류와 구미보 하류의 각각 세 지점에서 수행되었다. GPS부자는 바람에 의한 교란을 최소화하기 위해 지름 10 cm의 구형으로 제작하였으며 시범테스트를 통해 입자의 주 궤적 변화가 크지 않은 지점에 GPS부자를 투입했다. GPS부자는 오염물질의 사고유출을 가정하여 한 지점에 투입했고 GPS부자 사이의 간섭을 최소화하기 위해 25 ~ 35개의 GPS부자를 이용했다. 표면흐름에 의해 이동하는 부자의 위치는 GPS에 시계열로 저장됐고 ADCP를 이용하여 실험당시의 수리량을 측정했다. 입자위치의 시계열자료로부터 GPS부자의 확산범위의 시간변화를 계산했고 단순 모멘트법을 이용하여 종, 횡 방향 확산계수를 계산했다. 그 결과, 종 방향 확산계수는 $0.003{\sim}0.041m^2/s$로 계산되었고 횡 방향 확산 계수는 $0.001{\sim}0.012m^2/s$로 계산되어, 흐름방향의 유속성분에 의한 확산이 지배적인 것으로 나타났다. 지류 합류부에서는 이송이 지배적인 혼합이 발생되었고(Pe>1) Pe의 증가에 따라 수평확산계수가 감소되었다. 25~35개 GPS부자 궤적의 앙상블 평균으로부터 계산한 Integral time scale은 모멘트법으로부터 계산한 종, 횡 방향 확산계수와 비례하는 것으로 나타나, Taylor(1921)의 이론과 일치했다. 또한 실험수로에서 수행된 기존연구결과와 비교한 결과, 하폭 대 수심비, 마찰항의 증가에 따라 수평확산계수가 증가하는 경향을 나타내었다.