• 대한수의학회지
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• 제35권4호
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• pp.843-851
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• 1995

To observe the effect of Demodex canis infection on the cellular immune response and hematological profile, 8 Doberman pinschers experimentally infected with D cains and 4 uninfected control dogs were sensitized with 2, 4-dinitro-chlorobenzene(DNCB) on the skin and were challenged with DNCB 14 days after the initial sensitization to elicit allergic contact dermatitis. Histological and hematological changes of these dogs were then observed. Macroscopic changes of skin challenged with DNCB in D canis-infected dogs included significantly reduced area of allergic reaction(p<0.05) than in uninfected control group. Infiltration of inflammatory cells in the D canis-infected group was also significantly reduced(p<0.05) than in the uninfected control group. These changes indicated that the cell-mediated immune response of the animals was suppressed by the infection with D canis. Total white blood cell count in dogs infected with D canis was increased when dogs were sensitized with DNCB (p<0.01). The result appeared to be caused by stress due to D canis infection, secondary bacterial infection and decreased efficacy of general body defense system. Blood eosionophils were increased in D canis-infected dogs which appreared to be caused by the allergic contact dermatitis. Blood chemistry analysis revealed that total protein and globulin were increased(p<0.05), while albumin level was decreased. This result appeared to be caused by secondary bacterial infection.

• 한국임상수의학회지
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• 제37권2호
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• pp.96-99
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• 2020

An 11-year-old female Yorkshire terrier presented with alopecia, hyperpigmentation, and pruritus. Dermatological examination revealed a large number of Demodex mites including D. cornei and D. canis. Mean length of total body length of D. cornei was one third shorter than that of D. canis. In addition, sequential analysis of DNA extracted from the skin lesions showed a 97% and 99% identity with the two sequences of D. canis and D. cornei. This is the first report of a combined infestation of D. cornei and D. canis in Korea, along with its morphological and molecular characterization.

• Parasites, Hosts and Diseases
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• 제43권2호
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• pp.65-67
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• 2005

The intestines and hearts of dogs were examined for Toxocara canis, Toxascaris leonina, and Dirofilaria immitis, after necropsy between June 26 and September 29, 2004 in Chuncheon, Korea. Of the 662 dogs examined, 6 were infected with T. canis $(0.9\%),\;86\;with\;T.\;leonina\;(13.0\%)$. Fifty dogs were infected with D. immitis among 500 dogs examined $(10.0\%)$. Five were co-infected with T. canis and T. leonina, and three were co-infected with T. leonina and D. immitis. The cumulative positive infection rate for three species was $134/662(20.2\%)$. Considering previously reported seropositive rates of T. canis excretory-secretory antigen, i.e., $5\%$ in the adult population in Korea, the possibility of toxocariasis caused by T. leonina should be reevaluated.

• 한국동물위생학회지
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• 제33권1호
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• pp.29-35
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• 2010

This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

• Parasites, Hosts and Diseases
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• 제40권4호
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• pp.195-198
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• 2002

This study was performed to investigate whethere there is cross-reactivity between Dirofilaria immitis and three intestinal nematodes of dogs. In ELISA, D. immitis- infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of T. canis. In immunoblotting, some antigenic fractions, 44, 57 88, 100 kDa of crude extract of T. canis, were found to be positive reaction with sera of dogs infected with D. immitis. However, little or no cross-reaction were observed between sera of D. immitis-infected dogs and crude extract antigen of T. vulpis or A. caninum. These result suggest that there are partial cross reaction between sera of D. immitis-infected dogs and the antigen of T. canis.

• Parasites, Hosts and Diseases
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• 제45권1호
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• pp.19-26
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• 2007

This study describes the isolation of a Toxocara canis species-specific excretory-secretory(ES) antigen and the development of an enzyme-linked immunosorbent assay(ELISA) based on this antigen. Analysis of the ES antigens of T. canis, Toxocara vitulorum, Ascaris lumbricoides and Necator americanus larval antigen was performed by SDS-PAGE followed by western blotting. A 57 kDa T. canis-specific antibody fraction(TcES-57) was identified by western blotting and labelling with anti-Toxocara antibodies(from experimental rabbits and human patients) and tracing with anti-human or anti-rabbit peroxidase conjugate. No protein fraction of 57 kDa was detected in ES or larval antigens collected from T. canis, T. vitulorum, A. lumbricoides and N. americanus. Using TcES-57, a specific anti-serum was produced in rabbits and a double sandwich ELISA was developed. This test was validated using known seropositive sera from toxocariasis patients, sera from A. lumbricoides or N. americanus patients, and 50 serum samples from cats. These tests revealed that TcES-57 antigen is specific to T. canis infection and does not cross react with sera of other related infections. Thus, ELISA based on TcES-57 antigen was proven to be an effective tool in the diagnosis of toxocariasis and studies on the role of T. canis in the epidemiology of human toxocariasis.

• 대한수의학회지
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• 제41권2호
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• pp.167-172
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• 2001

1999년 3월부터 12월에 걸쳐 대구지방에서 피부사상균증에 이완된 개 110두에서 57주(51.8%)의 Microsporum canis를 분리하여 표준균주 1주와 함께 육안적, 현미경적 및 생물학적 성상을 조사하고 고찰하였다. M canis 57주는 집락의 성상 및 현미경적 소견에 따라 4가지(A~D)형으로 나눌 수 있었다. A형(white-yellow type)은 73%(42주)이며 표준주(ATCC 18615)의 전형적인 집락과 동일한 형이며, B형(fluffy type)은 21.1%(12주)이고, C형(flat type)은 3.5%(2주)이며, D형(radial groove type)은 1.7%(1주)이었으며, 이들 형은 전형적인 대분생자의 수적 차이가 인정되었다. 한편 계대가 진행됨에 따라 3가지 변이형(E~G)이 출현하였다. E형은 B형 12균주 중 25%(3주)가 변이한 균주로 갈색색소를 산생하고 비전형적인 대분생자가 관찰되며, G형은 B형의 변이주로 색소 및 대분생자가 소실된 형으로 25%(3주) 이었고, F형은 A형 42균주 중 7.1%(3주)가 변이한 균주로 섬유다발 모양의 성상과 뒷면의 색소 소실 및 비전형의 대분생자, 아령 모양의 균사가 관찰되었다. M canis 58주(표준주 포함)의 urease test, rice medium test, 모발천공시험에서 정도의 차이는 있었지만 전 균주가 양성이었다.

• Journal of Preventive Veterinary Medicine
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• 제43권4호
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• pp.214-220
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• 2019

Although canine brucellosis has been known to be an important re-emerging zoonosis, the pathophysiological mechanisms of Brucella canis infection remains clues to be solved. Different culture models, single and co-culture models, were constructed with canine epithelial cells, D17 and macrophage, DH82 to investigate the induction of immune responses in in vivo B. canis infection. Expression of genes related with induction of immune responses, Th1, Th2 and Th17, was compared in the two different models after the bacterial infection. In this study, expression of cytokine genes, IL-1β, IL-5, IL-6, IL-10, IL-23, and TNF-α was quantified in the DH82 at different time points using RT-qPCR in the two different culture systems after the infection. Cytokine genes related with Th1, IL-1β and TNF-α and Th17, IL-6 and IL-23 were expressed with time-dependent manners in the both systems (p<0.05). However, increase of Th2-related cytokine genes expression was not detectable in the both systems by comparison with control. The expression of Th1 and Th17 related cytokine genes was earlier in single cell culture than those in co-culture model (p<0.05). In general, amounts of the expressed genes were shown higher in single cell model than those in co-culture models. This study indicate that Th1 and Th17-associated immune responses are central to B. canis infection in dogs. In addition, it suggests a specific role of epithelial cells in the B. canis infection in vivo, which should resolved in the further study.

• 한국동물위생학회지
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• 제43권4호
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• pp.217-225
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• 2020

This study was conducted to survey pathogens of canine coronavirus (CCV), canine distemper virus (CDV), canine influenza virus (CIV), canine parvovirus (CPV), severe fever with thrombocytopenia syndrome virus (SFTSV), Dirofilaria (D.) immitis, Giardia and antibodies against Anaplasma (A.) phagocytophilum, Borrelia (B.) burgdorferi, Brucella (B.) canis and Ehrlichia (E.) canis among stray dogs in Gyeonggi province. We collected 271 feces, 291 bloods, 311 nasal and ocular swab samples from 311 of dogs in the Gyeonggi province assistance dogs sharing center from January to December, 2019. Among canine infectious disease pathogens, Giardia was highly detected in 46/271 (17.0%) samples. Subsequently, CCV 10.3% (28/271), D. immitis 8.2% (24/291), CPV 4.1% (11/271), CDV 1.0% (3/311), A. phagocytophilum (antibody) 0.3% (1/291), E. canis (antibody) 0.3% (1/291) were detected. Based on the results, this study is expected to provide a useful reference for disease control and management of stray dogs.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 1995년도 한국우주과학회보 제4권2호
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• pp.23-23
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• 1995

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