• 한국미생물·생명공학회지
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• 제18권1호
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• pp.49-55
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• 1990

D,L-2-aminothiazoline-4-carboxylic acid(D,L-ATC)로 부터 L-cysteine으로의 bioconversion에 대한 특성을 살펴보았다. Pseudomonas species의 배양중에 D,L-ATC를 첨가하여 균체내에 그 관여되는 효소를 유도, 생성시키고 균체만을 모은 후 파쇄하여 조효소액을 제조하였다. 실험결과, DL-ATC로 부터 L-형의 cysteine 만이 생성되며, 이 반응에 관여되는 효소는 cofactor로서 Mn이온을 필요로 하며, Mn 이온의 첨가에 의해 L-cysteine의 생성량이 수십배 증가되었다. 그러나, 이 효소는 생성물인 L-cysteine에 의해 feedback inhibition을 받았다. 한편, L-cysteine의 분해효소가 조효소액 내에 존재하며 그 효소반응의 저해제없이는 생성된 L-cysteine의 대부분이 분해되었다. 반면, 매우 효과적인 효소저해제인 hydroxylamine의 첨가로 L-cysteine의 분해를 거의 방지할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제1권1호
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• pp.50-53
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• 1991

The reaction steps involved in the bioconversion of a chemically synthesized precursor, $D,L-2-amino-{\Delta}^2-thiazoline-4-carboxylic$ acid (D,L-ATC), to L-cysteine and the properties of the involved enzymes were investigated. It was found that the conversion consisted of two steps, i. e., D,L-ATC to S-carbamyl-L-cysteine (S-C-L-cysteine) and S-C-L-cysteine to L-cysteine, and the S-C-L-cysteine was an intermediate between them. While the enzymes involved in the reactions were induced by the addition of D,L-ATC as an inducer, S-C-L-cysteine induced only the enzyme involved in the latter step. The conversion of S-C-L-cysteine to L-cysteine could be also carried out in the presence of hydroxylamine and its rate was much faster than that by the corresponding enzyme. On the other hand, L-cysteine (or L-cystine) was decomposed to evolve $H_2S$ by the enzyme considered to be a kind of desulfhydrase. However, hydroxylamine was a perfect inhibitor for this enzyme.

• 한국미생물·생명공학회지
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• 제20권6호
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• pp.681-686
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• 1992

D,L-ATC의 L-cysteine으로의 생물학적 전환에 있어 균체를 이용할 때, 계면활성제의 영향, 효소의 안정성 및 연속생산공정중의 고정화 균체 반응기의 안정성에 대하여 분석하였다. 계면활성제의 첨가없이 균체만을 이용할 때 반응은 매우 미미하게 이루어졌으나 SDS와 Triton X-100을 첨가할 때 cell-free 조효소액을 이용하는 경우와 비슷한 정도의 결과가 얻어졌다. 효소 활성은 $30^{\circ}C$에서 7시간 저장후 50 저하되었으며 질소가스하의 혐기적인 조건에서는 활성저하가 거의 일어나지 않았다. 이와 같은 효소의 불활성화는 효소에 대한 산소의 작용으로 사료되었다. 그러나, alginate로 고정화한 균체를 이용한 연속반응공정에서 혐기적으로 조건하에서 150시간 내에 대부분의 활성을 잃어버렸으며, L-cysteine 저해제인 hydroxylamine을 첨가할 때 효소활성이 급속히 감소되었다.

• 한국항공운항학회지
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• 제11권2호
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• pp.59-77
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• 2003

Air Traffic Control Tower is one of the most important units in Airport operation. It provides services related to safe and efficient traffic flows that control aircraft on the ground maneuvering area and terminal airspace. Also It is responsible for managing of ground operators. The major objective of this study is to evaluates relative efficiency of ATC towers in Domestic airports using data envelopment analysis so that it helps the ATC authority to improve the tower efficiency, to decide the level of benchmarking target and to establish the best alternative. The results of this study are the following; First, as a result of analysis for the potential improvement, it has analysed that the common problems of each ATC tower are to increase its number of flight and to reduce its number of runway followed by airside area, the number of air traffic controller and the number of stand. Second, it has shown that the each tower in RKPC(8), RKPT(5), RKPK(l) and RKSS(l) are used as the reference set. Especially, the tower in RKPC analyzed as a relatively efficient unit is the most main target for the towers in RKTU, RKTH, RKPS and RKTY to do bench marking and to set up the strategy for improving relative efficiency of the tower. Third, tower is actually not able to control the input and output data in this study except the number of controller, so that ATC authority is recommended to improve inefficiency of the towers through handling the number of controller.

• Fisheries and Aquatic Sciences
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• 제25권3호
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• pp.158-166
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• 2022

The complete mitochondrial genome of Tremoctopus violaceus was sequenced to analyze its organization and phylogenetic status within the order Octopoda. The mitochondrial genome of T. violaceus had a structure and organization similar to that of other Octopoda. The content of the nucleotides A, C, G, and T was 31.68 %, 7.71 %, 20.02 %, and 40.58 %, respectively. All protein-coding genes (PCG) began with the ATG codon, excluding ND4 and ATP6, which began with ATC and ATT, respectively, and terminated with TAG, TAA, TA, or T. Codons for isoleucine were the most used codons, whereas those for arginine were used the least. Two extra tRNAs, trnN and trnL, were found in the control region. These tRNAs have a D-armless structure. The control region had excess A + T content (83.16 %) and a stem-loop structure with two elements, which is reported for the first time in Octopoda by our study. Bayesian inference using 13 PCG revealed that Octopus and Octopodidae were polyphyletic, and that Tremoctopodidae diverged relatively earlier within Octopoda. The mitochondrial genome of T. violaceus and its characteristics may help to understand the evolutionary history of Octopoda and establish a marine biodiversity conservation strategy.

• 자원리싸이클링
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• 제8권5호
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• pp.34-43
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• 1999

폐윤활유로부터 활융가능한 연료기체를 생산하기 위해 개발한 전기아크를 이용한 분해자치에서 폐윤활유를 아크 분해할 때의 기체생성물과 잔류물의 특성을 연구하였다. 생성되는 기체는 수소(35~40%)와 아세틸렌(13~20%), 에틸렌(3~4%), 그 외 탄산수소들로 이루어져 있으며, 저온 열분해에서는 주요 생성물인 일산화탄소의 함량은 매우 적었다. 발생기체의 발열량은 11,000~13,000kacl/kg으로 기체 연료로의 활용성이 충분하여 유해기체도 배출기준치 이하였다. 액상 잔류물을 GC/MS로 분석한 결과 폐유의 고분자량 탄화수소물들이 저분자량 탄화수소물과 수소로 분해되었음을 알 수 있었고, 전지아크에 의한 고온 열분해이므로 탈수소반응이 주 반응임을 확인할 수 있었다. 고상 잔류물인 검댕이 평균 입경은 $10.3\mu\;extrm{m}$이며, 탄소 함량은 60%이상이고, 중금속 성분은 0.01ppm 이하였는데 전제과정을 거치 재활용이 가능할 것으로 생각된다.