• Nuclear Engineering and Technology
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• 제45권5호
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• pp.653-664
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• 2013

The design of computer-based instrumentation and control (I&C) systems is determined by the allocation of I&C functions to I&C systems and components. Due to the characteristics of computer-based technology, component failures can negatively affect several I&C functions, so that the reliability proof of the I&C systems requires the accomplishment of I&C system design analyses throughout the I&C life-cycle. On one hand, this paper proposes the restructuring of the sequential IEC 61513 I&C life-cycle according to the V-model, so as to adequately integrate the concept of verification and validation. On the other hand, based on a metamodel for the modeling of I&C systems, this paper introduces a method for the modeling and analysis of the effects with respect to the superposition of failure combinations and event sequences on the I&C system design, i.e. the temporal change of physical structure is analyzed. In the first step, the method is concerned with the modeling of the I&C systems. In the second step, the method considers the analysis of temporal change of physical structure, which integrates the concepts of the diversity and defense-in-depth analysis, fault tree analysis, event tree analysis, and failure mode and effects analysis.

• 연구논문집
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• 통권27호
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• pp.75-86
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• 1997

Combined cycle power plant is a system where a gas turbine or steam turbine is used to produce shaft power to drive a generator for producing electrical power and the steam from the HRSG is expanded in a steam turbine for additional shaft power. Combined cycle plant is a one from of cogeneration. The temperature of the exhaust gases from a gas turbine ranges from $400^\circC$ to $600^\circC$, and can be used effectively in a heat recovery steam generator to produce steam. Combined cycle can be classed as a "topping(gas turbine)" and a "bottoming(steam turbine)" cycle. The first cycle, to which most of the heat is supplied, is called the topping cycle. The wasted heat it produces is then utilized in a second process which operates at a lower temperature level and is therefore referred to as a "bottoming cycle". The combination of gas/steam turbine power plant managed to be accepted widely because, first, each individual system has already proven themselves in power plants with a single cycle, therefore, the development costs are low. Secondly, the air as a working medium is relatively non-problematic and inexpensive and can be used in gas turbines at an elevated temperature level over $1000^\circC$. The steam process uses water, which is likewise inexpensive and widely available, but better suited for the medium and low temperature ranges. It, therefore, is quite reasonable to use the steam process for the bottoming cycle. Only recently gas turbines attained inlet temperature that make it possible to design a highly efficient combined cycle. In the present study, performance analysis of a dual pressure combined-cycle power plant is carried out to investigate the influence of topping cycle to combined cycle performance.

• Journal of Microbiology and Biotechnology
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• 제13권4호
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• pp.607-612
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• 2003

To elucidate the action mechanism of MCS-C2, a novel analogue of toyocamycin and sangivamycin, its effect on the expression of cell cycle-related proteins in the human myelocytic leukemia cell line HL-60 was examined using Western blotting and a flow cytometric analysis. MCS-C2, a selective inhibitor of cyclin-dependent kinases, was found to inhibit cell growth in a time- and dose-dependent manner, and inhibits cell cycle progression by inducing the arrest at G1 and G2/M phases, in HL-60 cells. The flow cytometric analysis revealed an appreciable arrest of cells in the G2/M phase of the cell cycle after treatment with MCS-C2. The HL-60 cell population increased gradually from 13% at 0 h, to 28% at 12 h in the G2/M phase, after exposure to $2{\;}\mu\textrm{M}$ MCS-C2. Furthermore, Western blot analysis demonstrated that MCS-C2 induced the cell cycle arrest at G1 phase through the inhibition of pRb phosphorylation. Hypophosphorylated pRb accumulated after treatment with $5{\;}\mu\textrm{M}$ MCS-C2 for 12 h, whereas, the level of hyperphosphorylated pRb was reduced. Thus, treatment of the cell with MCS-C2 suppressed the hyperphosphorylated form of pRb with a commensurate increase in the hypophosphorylated form.

• Journal of Ginseng Research
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• 제20권3호
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• pp.219-225
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• 1996

This study was performed to investigate the inhibition mechanism of cancer cell proof iferation caused by the petroleum-ether extract of ginseng against human rectum (HRT-18), colon (HT-29), llepatoma (Hep G2) and prostate (LNCaP) cancer cells and monkey kidney cells (Vero 76). Cells were treated with the petroleum-ether extract of ginseng (50 to 200 $\mu\textrm{g}$/ml) in G1 or S phase of the cell cycle, and proliferation and protein kinase C activity were measured. The petroleum-eth or extract of ginseng inhibited proliferation of HRT-18, HT-29, Hep G2 and LNCaP when treated in Gl phase, but not in S phase. This result shows that the ginseng extract arrests the cell cycle in G1 phase, resulting in the inhibition of cell proliferation. At the same concentrations, treatment of the ginseng extract in G1 phase decreased protein kinase C activity, while the treatment in S phase had no effect. This reault suggests that protein kinase C might be involved in the inhibition of the cell cycle and proliferation of cancer cells caused by the petroleum-ether extract of ginseng.

• 한국추진공학회지
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• 제2권2호
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• pp.74-82
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• 1998

복합발전 사이클은 가스터빈이나 스팀터빈으로부터의 출력을 이용하여 전개를 생산하기 위한 발전기를 구동시키고 배영회수기로부터 나온 증기를 스틸터빈에서 팽창시킴으로서 부가적인 동력을 얻는 장치를 가리킨다. 보통 가스터빈 배기로 부터의 온도는 $400{\sim}650^{\circ}C$정도로서 배열회수기에서 효과적으로 스팀을 생산할 수 있는 수준의 온도이다. 복합 사이클은 일반적으로 상부사이클과 하부사이클로 구분하는데 대부분의 열에너지 공급이 이루어지는 상부사이클을 브레이돈사이클 이라하며 브레이돈사이클에서 소비되는 에너지는 보다 낮은 온도 수준인 하부사이클에서 회수된다. 이러한 복합사이클은 최근 들어 더욱 보편적으로 적용되고 있는데 그 이유는 첫째, 가스터빈이나 스팀터빈이 독자적으로도 충분히 기술적인 검증을 받은 열기관으로서 초기에 비해 개발비가 저렴해졌다는 데 있고, 둘째, 작동유체인 공기가 $1000^{\circ}C$ 이상에서도 별다른 문제없이 적용될 수 있는 안전한 유체이고 비용이 전혀 들지 않는다는 점이다. 그 뿐 아니라 스팀터빈에 사용되는 물도 중저온에서 매우 저가로 공급할 수 있고 쉽게 공급이 가능하다는 이점으로 하부사이클에의 적용이 매우 양호하다는 점이다. 최근 소재기술의 개발에 따른 터빈입구온도의 향상은 이러한 복합발전 사이클의 기술적, 경제적 이점을 더욱 강화시켜 주고 있다. 본 연구에서는 3압에 의한 복합사이클에 대한 성능해석을 통하여 상부사이클이 전체 복합발전 성능에 미치는 영향을 조사하였으며 그 결과를 서인천 복합발전 인수 성능시험결과와 비교하였다. 본 연구결과는 현재 개념설계가 이루어지고 있는 장차 150~200MW수준의 산업용 가스터빈 개발에 중요한 방향제시를 할 수 있을 것으로 판단된다.

• Molecular & Cellular Toxicology
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• 제2권3호
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• pp.159-165
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• 2006

To determine the anticancer effect of D-amygdalin (D-mandelinitrole-${\beta}$-D-gentiobioside) in human chronic myeloid leukemia cells K562, we profiled the gene expression between amygdalin treatment and control groups. Through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of D-amygdalin was $57.79{\pm}1.83%$ at the concentration of 5 mg/mL for 24 h. We performed cDNA microarray analysis and compared the gene expression profiles between D-amygdalin (5 mg/mL, 24 h) treatment and control groups. Among the genes changed by D-amygdalin, we paid attention to cell cycle-related genes, and particularly cell cycle regulator genes; because arrest of cell cycle processing was ideal tactic in remedy for cancer. In our data, expressions of cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), ataxia telangiectasia mutated (includes complementation groups A, C, and D) (ATM), cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), and CHK1 checkpoint homolog (CHEK1, formally known as CHK1) were increased, while expressions of cyclin-dependent kinase 2 (CDK2), cell division cycle 25A (CDC25A), and cyclin E1 (CCNE1) were decreased. The pattern of these gene expressions were confirmed through RT-PCR. Our results showed that D-amygdalin might control cell cycle regulator genes and arrest S phase of cell cycle in K562 cells as the useful anticancer drug.

• 한국재료학회지
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• 제8권10호
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• pp.945-949
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• 1998

Ta(OC2H5)5와 NH3를 이용하여 Cycle-CVD법으로 산화탄탈륨 막을 증착하였다. Cycle-CVD법에서는 Ta(OC2H5)5와 NH3사이에 불활성 기체를 주입한다. 하나의 cycle은 Ta(OC2H5)5주입, Ar주입, NH3 주입, Ar 주입의 네 단계로 이루어진다. Cycle-CVD법으로 산화탄탈륨 막을 증착할 때, 온도 $250-280^{\circ}C$에서 박막의 증착 기구는 원자층 증착(Atomic Layer Deposition:ALD)이었다. $265^{\circ}C$에서 Ta(OC2H5)5:Ar:NH3:Ar:NH3:Ar의 한 cycle에서 각 단계의 주입 시간을 1-60초:5초:5초:5초로 Ta(OC2H5)5 주입 시간을 변화시키면서 산화탄탈륨 막을 Cycle-CVD법으로 증착하였다. Ta(OC2H5)5주입시간이 증가하여도 cycle 당 두께가 $1.5\AA$/cycle로 일정하였다. $265^{\circ}C$에서 증착된 박막의 누설 전류는 2MV/cm에서 2x10-2A/$\textrm{cm}^2$이었고 열처리후의 산화탄탈륨 막의 누설 전류값은 $10-4A\textrm{cm}^2$ 이하고 감소하였다. 증착한 산화탄탈륨 막의 성분을 Auger 전자 분광법으로 분석하였다. 2$65^{\circ}C$에서 증착한 막의 성분은 탄탈륨 33at%, 산소 50at%, 탄소 5at%, 질소 12at% 이었으며 90$0^{\circ}C$, O2300torr에서 10분 동안 열처리한 박막은 탄탈륨 33at%, 산소 60wt%, 탄소 4at%, 질소 3at%이었다. 박막의 열처리 온도가 높을수록 불순물인 탄소와 질소의 박막 내 잔류량이 감소하였다. 열처리 후의 박막은 O/Ta 화학정량비가 증가하였으며 Ta의 4f7/5와 4f 5/2의 결합 강도가 열처리 전 박막보다 증가하였다. 열처리 후 누설 전류가 감소하는 것은 불순물 감소와 화학정량비 개선 및 Ta-O 결합 강도의증가에 의한 것으로 생각된다.

• Nuclear Engineering and Technology
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• 제38권2호
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• pp.109-118
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• 2006

Various indirect power cycle options for a helium cooled gas cooled fast reactor (GFR) with particular focus on a supercritical $CO_2(SCO_2)$ indirect cycle are investigated as an alternative to a helium cooled direct cycle GFR. The balance of plant (BOP) options include helium-nitrogen Brayton cycle, supercritical water Rankine cycle, and $SCO_2$ recompression Brayton power cycle in three versions: (1) basic design with turbine inlet temperature of $550^{\circ}C$, (2) advanced design with turbine inlet temperature of $650^{\circ}C$ and (3) advanced design with the same turbine inlet temperature and reduced compressor inlet temperature. The indirect $SCO_2$ recompression cycle is found attractive since in addition to easier BOP maintenance it allows significant reduction of core outlet temperature, making design of the primary system easier while achieving very attractive efficiencies comparable to or slightly lower than, the efficiency of the reference GFR direct cycle design. In addition, the indirect cycle arrangement allows significant reduction of the GFR &proximate-containment& and the BOP for the $SCO_2$ cycle is very compact. Both these factors will lead to reduced capital cost.

• Journal of Welding and Joining
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• 제14권1호
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• pp.38-46
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• 1996

High temperature low cycle fatigue crack growth behavior is investigated over a range of two temperatures and various frequencies in SUS 304 stainless steel. It is found that low frequency and temperature can enhance time-dependent crack growth. With high temperature, low frequency and long crack length, ${\Delta}J_c/{\Delta}J_ f$, the ratio of creep J integral range to fatigue J integral range is increased and time-dependent crack growth is accelerated. Interaction between ${\Delta}J_f$ and ${\Delta}J_c$ is occured at high frequency and low temparature and ${\Delta}J_c$, creep J integral range is fracture mechanical parameter on transition from cycle-dependent to time dependent crack growth in creep temperature region.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2012년 춘계학술대회
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• pp.221-232
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• 2012

Cycle analysis has been performed to find out the optimum design point of the BOG re-liquefaction plant. The cycle state, defined by three cycle variables, was mainly described by the three cold temperatures of the three-pass heat exchanger, on which the constraints by the heat exchanger are imposed. The cycle states which are confined within a domain limited by the temperature constraints were the primary issue of this study. The BOG mass within the domain was analyzed first and then the cycle performance was related to the BOG mass afterwards, which enabled us to explain the observed behavior of the cycle performance under the temperature constraints by the heat exchanger. A good cycle performance could be ensured if the two cold Nitrogen temperatures of the three temperatures were placed close together near $-140^{\circ}C$ while the BOG temperature is kept far above enough, but not too far, from $-140^{\circ}C$ such that it does not interfere in their optimum temperature range.