• Biomolecules & Therapeutics
• /
• v.27 no.3
• /
• pp.311-317
• /
• 2019

Mast cells are the most prominent effector cells of Type 1 hypersensitivity immune responses. CYC116 [4-(2-amino-4-methyl-1,3-thiazol-5-yl)-N-[4-(morpholin-4-yl)phenyl] pyrimidin-2-amine] is under development to be used as an anti-cancer drug, but the inhibitory effects of CYC116 on the activation of mast cells and related allergy diseases have not reported as of yet. In this study, we demonstrated, for the first time, that CYC116 inhibited the degranulation of mast cells by antigen stimulation ($IC_{50}$, ${\sim}1.42{\mu}M$). CYC116 also inhibited the secretion of pro-inflammatory cytokines including TNF-${\alpha}$ ($IC_{50}$, ${\sim}1.10{\mu}M$), and IL-6 ($IC_{50}$, ${\sim}1.24{\mu}M$). CYC116 inhibited the mast cell-mediated allergic responses, passive cutaneous anaphylaxis (ED50, ~22.5 mg/kg), and passive systemic anaphylaxis in a dose-dependent manner in laboratory experiments performed on mice. Specifically, CYC116 inhibited the activity of Fyn in mast cells and inhibited the activation of Syk and Syk-dependent signaling proteins including LAT, $PLC{\gamma}$, Akt, and MAP kinases. Our results suggest that CYC116 could be used as an alternative therapeutic medication for mast cell-mediated allergic disorders, such as atopic dermatitis and allergic rhinitis.

• Proceedings of the KSME Conference
• /
• 2001.11a
• /
• pp.772-777
• /
• 2001

The gas-assisted injection molding process is often perceived to be unpredictable, because of the extreme sensitivity of the gas. Since a slight change in design or process parameters can significantly change the resulting gas penetration, few designers and molders have the level of experience with the new gas-assisted injection molding process required for the development of new parts. This paper is concerned with the unified molding for a thick cosmetic chest by using gas-assisted injection molding. CAE analysis was carried out to design the part and the gas channel without inducing sink marks. And based on the part weight measurement, the processing parameters to control gas penetration percentage were chosen through the method of design of experiments. A thick cosmetic chest was successfully produced using the gas assist technology. The sink mark issue associated with the conventional injection molded parts was resolved. Weight savings and cycle-time reduction were also achieved.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1996.11a
• /
• pp.806-809
• /
• 1996

The present development relates to an apparatus for loading or unloading a workpiece, and more particularly, to an apparatus for loading or unloading a workpiece from a press die or supplier An apparatus for loading or unloading a workpiece has been installed in a press in order to load or unload a workpiece from a press die. There has been known such a loading or unloading apparatus as the apparatus can feed a workpiece in not only the horizontal direction but also in the vortical direction. Press working system in automotive factories are now switching over to a transfer press work ing system. The current problem to be sieved immediately is to automate the existing manual press lines for the manpower saving and speedy production. “Automation system for press line”has just been developed to meet the above trend. The loader and unloader are development under the design concept for high performance, easy control and operational safety to meet the more extensive.