• Microbiology and Biotechnology Letters
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• v.9 no.1
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• pp.7-19
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• 1981

The production of gluconic acid from glucose by the resting cell system of Aspergillus niger was studied. It was found that the conversion products from glucose by the resting cell system were markedly influenced by the pH, temperature, substrate concentration, aeration, metal ions, cultivation time and storage conditions of the resting cells. Conversion products were identified as gluconic acid by the thin layer chromatography and infrared spectrophotometry. These conversions were greatly stimulated by addition of $Mg^{++}$, and S $n^{++}$, but showed inhibitory effects by C $u^{++}$, H $g^{++}$, C $d^{++}$, A $g^{+}$ and cyanide. For the optimum cell storage, it was effective to be kept at -$25^{\circ}C$ in 0.05M phosphate buffer solution of pH 7.0. The gluconic acid production by the resting cell system was more effective than those of the fermentation with respect to cultivation time, yield, recovery and re-use of the cell.l.l.l.l.l.l.

• Applied Biological Chemistry
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• v.30 no.3
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• pp.219-226
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• 1987

Peroxidase isozyme C was isolated from mung bean cotyledon and purified to homogeneity as ascertained by chromatography and polyacrylamide gel electrophoresis, and then crystallized. Purification procedures included ammonium sulfate precipitation and column chromatography on Sephadex G-75, DEAE-cellulose and DEAE-Sephadex A-50. Peroxidase isozyme C was purified about 63 fold with 5% recovery. Isozyme C showed optimal activity at pH 5.0 with o-dianisidine and at pH 6.0 with guaiacol as substrate, and the optimal temperature was $70^{\circ}C$. Molecular weight of 50,000 was estimated for the isozyme C by SDS-polyacrylamide gel electrophoresis. At $70^{\circ}C$, it took 30 min to inactivate the isozyme to 50%, and at $80^{\circ}C$, this isozyme was almost completely inactivated in 20 min. The Km value of isozyme C for o-dianisidine was 0.11mM and that for guaiacol was 60.98mM using hydrogen peroxide as cosubstrate, and the kinetic pattern showed a competitive cyanide inhibition with respect to substrate. The crystalline structure of isozyme C was rectangular in shape.

• Journal of the Korean Society of Marine Environment & Safety
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• v.7 no.1
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• pp.7-14
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• 2001

The amount of petroleum consumption has been Increased according to the industrialization and It leads to the increase of the possibility of marine oil pollution. In Korea, some countermeasures including oil skimmer, gelling agent and herding agent of oil have been used for the remediation of the pollution. However, most of them have lets of shortcomings in the application under in-situ condition, because they are sensitive to the situation such as geographical feature, the wind and the tide. In reported literature, the natural powdered oil absorbent which is made of peat moss is an effective mean to clean spilled oil from lake or coast. However, the peat moss is a natural resource which is only Produced from a specific cold weather are like Canada. This indicates that the alternative materials which is readily obtained from everywhere are needed for powdered oil absorbent. Therefore. in the study, same natural materials including pine leaves and straw are tested as the alternative materials for the absorbent. The raw materials were dried and treated by heat at various temperature during several Periods and then. shattered by a grain cracking machine. The oil sorption capacity of the prepared materials was compared according to the methods of heat treatment and their sizes. The proportion of hydrogen cyanide to combustion of the absorbents was measured to confirm their final disposal methods. The biodegradability test of the absorbents was carried our to evaluate possibility of a side pollution in the coast. In was found that the heat treatment of pine leaves enhanced the capacity of oil sorption and decreased the water sorption. The maximum oil sorption was observed for the material treated at 18$0^{\circ}C$for 60 min. The amount of hydrogen cyanide from the combustion were 0.09ml/g, 0.07ml/g for pine leaves and straw respectively meaning that the final disposal by combustion might be feasible. The amount or organic carbon extracted from pine leaves during 7 days was up to 0.015g organic carbon from one gram of pine leaves. but the degradation was as fast as for glucose. It is concluded that the pine leaves can be served as a good raw material for the powdered oil absorbent like peat moss.

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.2
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• pp.110-117
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• 1987

Polyphenol oxidase in Prunus salicina(Red) was extracted, some properties and its partially purification were investigated as follows; Polyphenol oxidase was purified about 15 folds after ammonium sulfate saturation and about 64 folds after Sephadex G-100 column chromatography. Polyphenol oxidase showed optimum pH for activity at 6.5 and optimum temperature at at $35^{\circ}C$ and high affinity to catechol in o-diphenol compounds. Thermal stability were about 85% and 75% of initial polyphenol oxidase activity remained after heating at $50^{\circ}C$ for 5 minutes and 30 minutes respectively. The Michaelis constant of the enzyme was 2.58mM. L-cysteine, glutathione, ascorbic acid and potassium cyanide appeared to be most effective inhibitors. EDTA showed a slight inhibition.

• The Plant Pathology Journal
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• v.34 no.3
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• pp.208-217
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• 2018

In this study, samples were collected from the leaves and stems of healthy wild Pistachio trees (Pistacia atlantica L.) from various locations of Baneh and Marivan regions, Iran. In total, 61 endophytic bacteria were isolated and grouped according to phenotypic properties. Ten selected isolates from each group were further identified by partial sequencing of the 16S rRNA gene. Based on the results, isolates were identified as bacteria belonging to Pseudomonas, Stenotrophomonas, Bacillus, Pantoea and Serratia genus. The ability of these isolates was evaluated to phytohormone production such as auxin and gibberellin, siderophore production, phosphate solubilization, atmospheric nitrogen fixation, protease and hydrogen cyanide production. All strains were able to produce the plant growth hormone auxin and gibberellin in different amounts. The majority of strains were able to solubilize phosphate. The results of atmospheric nitrogen fixation ability, protease and siderophore production were varied among strains. Only Ba66 could produce a low amount of hydrogen cyanide. The results of biocontrol assay showed that Pb78 and Sp15 strains had the highest and lowest inhibition effects on bacterial plant pathogens, Pseudomonas syringae pv. syringae Pss20 and Pseudomonas tolaasii Pt18 under in vitro condition. Pb3, Pb24 and Pb71 strains significantly promote root formation on carrot slices. To our knowledge this is the first report of the isolation of endophytic bacterial strains belonging to Pantoea, Bacillus, Pseudomonas, Serratia and Stenotrophomonas genus from wild pistachio trees with plant growth promoting potential and biocontrol activity.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1424-1432
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• 2007

Two male, rumen fistulated crossbred Brahman-Thai native beef cattle (body weight = $400{\pm}50$ kg), fed on rice straw as a source of roughage, were used as rumen fluid sources. The treatments were $2{\times}3$ factorial arrangements; two roughages (fresh cassava foliage and cassava hay) and three sulfur levels (elemental sulfur) at 0.2 (control), 0.5 and 1% of DM, respectively. The experiment revealed that the rates (c) of gas production, ammonia-nitrogen concentration, true digestibility, total concentration or molar proportions of VFA and microbial biomass were not significantly different between cassava hay and fresh cassava foliage. However, all parameters for cassava hay were higher than for fresh cassava foliage. The supplementation of 0.5% sulfur to fresh cassava foliage resulted in a significant increase in the rate of gas production, true digestibility, total concentration of VFA, microbial biomass, rate of HCN disappearance, thiocyanate appearance and cyanide percentage conversion into thiocyanate. However, there were no effects of sulfur supplementation at 0.2, 0.5 and 1% to cassava hay. The finding suggests the utilization of cassava foliage for rumen microorganisms in terms of fermentation and HCN detoxification could be improved by sulfur supplementation of 0.5% of DM.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.188-193
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• 1997

Panax ginseng C.A.Meyer,1 to 5 years old were electrophored and were stained for SOD activity. The result indicated a total of 13 distinct form of the enzyme and the pattern of achromatic bands were not different according to ages. Nine of the enzyme activities were eliminated with cyanide or peroxide treatment and were resistant to treatment of chloroform plus ethanol. It suggested that they may be cupro-zinc containing SOD, whereas four were cyanide or peroxide resistant and were eliminated with cholroform plus ethanol treatment. They may be manganese containing SOD. Ginseng roos. 1 to 5 years old were analyzed for their SOD measurement of SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of about 700-800 unit/g of fresh weight. Therefore, the SOD activities from SOD-mimic compounds were higher than one from SOD. The ratio between the SOD activity from SOD-mimic compounds and one from true SOD was approximately 2:1 to 3:1.

• Journal of Korea Soil Environment Society
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• v.2 no.3
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• pp.39-47
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• 1997

Geochemical study was carried out to find out the distribution of metals and cyanide in soil in the vicinity of the abandoned Daduck mine and the reason for acid mine drainage occurrence in the tailings impoundment. Chemical analysis showed that content of As in soil around tailings exceeded 15mg/kg, Korean standard of soil contamination in the farm land. That means the contamination of soil by As is due to input of tailings. According to total decomposition of tailings, As, Cd, Cu, Pb, Zn and S were highly concentrated in tailings. However the water in tailings impoundment was changed to acidic and contaminated by metals and sulfate because the tailings in the top of the tailings impoundment had been oxidized. Acid mine drainage contaminated the water course in the vicinity of the paddy soils. The proper measures are required to prevent contamination of the soil and water in the vicinity of the Daduck mine.

• Journal of the Korean institute of surface engineering
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• v.1 no.1
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• pp.5-13
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• 1967

Up to this date, numerous methods of analysis of electropling solutions are published. Some, however, need lots of works before reaching final results, or require high technique and special instruments, and also some are unaccurate due to unclearnes of end point. Like our undevelop countries, technicians of electroplating shops are most high school graduates or under, and have not much knowledge on chemistry. Furthermore, those technicians have to control their plating solutions by themselves without having enough analytical laboratory equipment . Therefore, in this paper the simplest, besides accurate method is investigated after comparing numerous methods published. Among the methods of copper determinations from acid and alkaline copper plating baths, EDTA titration method are chosen, due to these methods are the simpest and fastest for the evaluation of metal content, without requirng any special instrument. For acid copper solutions, chelate titrations were accurate enough. Since the end point of titration of chelate method is variable according to the kind of indicators and other metal's coexisitence as well as solution component, many difficulties were encountered from cyanide copper, on the contrary of acid copper bath. PAN , PV, and MX indicators were tried , but it is found that MX is the best. In chyanide solution ,due to cyanide is the masking reagent , elimination of this component is essential , and finally found that elimination CN-by precipitation with AgNO$_3$ solution was the simplest and the most accurate way among others. This method was very accurate for the new plating solutions even coexistence with organic brightners. However used solutions for long months running have to be predetermined the accurate copper value by thiosulfate method form time to time, before chelate titration by means of AgNO$_3$ precipitation. Always some constant deviations will be seen according to the solutions nature. Therefore those deviation values have to be compensated each time.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.2
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• pp.171-177
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• 2005

Coconut based activated carbon and silica-gels were impregnated with 3-aminopropyltri ethoxysilan(APS) and N-(2-aminoethyl)-3-aminopropyl triethoxysilane (AEAPS) in order to investigate the effect of the amine group and the pore size of the supports on the removal of hydrogen cyanide(HCN) and aldehydes in mainstream smoke(MS). The physicochemical properties of the supports were analyzed by using thermal gravity analyzer(TGA), $N_2$ adsorption and desorption isotherms$(BET,\;N_2)$, and SEM-EDS. According to our experimental data, there was no significant difference in the delivery amount of HCN and aldehydes of non-functionalized silica-gels having meso-pores bigger than $20\AA$. In the case of silica-gels functionalized with APS(APS silica-gel), the delivery amounts of hydrogen cyanide(HCN) and aldehydes decreased with the increase of APS concentration. Silica-gel functionalized with AEAPS(AEAPS silica-gel) showed higher removal efficiency than that of APS silica-gels. The delivery amounts of HCN and aldehydes of activated carbon impregnated with APS and AEAPS increased with the increase of the APS and AEAPS concentrations. In accordance with the specific surface area analysis results, APS and AEAPS molecules decreased the specific surface area by blocking the micro-pores of the activated carbon. The volatile organic components removal efficiency by the micro-pores was higher than that of the amine group impregnated into the activated carbon.