• Archives of Reconstructive Microsurgery
• /
• 제6권1호
• /
• pp.1-8
• /
• 1997

Recently prostaglandin $E_1(PGE_1)$ has been shown to ensure flap survival by producing vasodilation of the peripheral vessels and platelet disaggreation. However, direct observation and detailed quantitative studies of the effects of $PGE_1$ on the cutaneous microcirculation have not been reported. In the present study, we investigated cutaneous microcirculatory changes in the rabbit ear chamber(REC) with an intravital microscope following intravenous administration of $PGE_1$. The results obtained in this study indicate that $PGE_1$ administered intravenously at a rate of 200ng/kg/min might act directly on the vessels and cause dilatation of metarterioles and capillaries without affecting vasomotion and systemic blood pressure. Clinically in order to evaluate the effect of an intravenous administration of $PGE_1$ on the cutaneous microcirculation, cutaneous blood flow, skin temperature and transcutaneous $Po_2$ in the pedicle or free flap of operated patients were evaluated by the combination of several measurements following the administration of $PGE_1$. The present study suggests that improvement of cutaneous microcirculation by $PGE_1$ may enhance the survival rate of flap or replantation. Both vessel arterial ischemia and venous congestion are main factors of tissue necrosis in the flap surgery. Vasodilatory or antithrombotic agents have been used in salvage of flap necrosis. However, the therapeutic effects of those drugs are still not well elucidated. Recently prostaglandin $E_1(PGE_1)$ has been shown to ensure flap survival by producing vasodilatation of the peripheral vessels and platelet disaggregation[1-3]. Emerson and sykes[4] have obtained significant improvement in the flap survival in the rat using $PGI_2$. Suzuki et al.[5] have reported prolonged flap survival length by using $PGE_1$ in the rabbit and concluded that $PGE_1$ improved the microcircuration in the flap. However, direct observation and detailed quantitative studies of the effects of $PGE_1$ on the cutaneous microcirculation have not been reported. In the present study, we investigated microcirculatory changes in the rabbit ear chamber[6,7] with an intravital microscope following intravenous administration of $PGE_1$.

• 한국임상수의학회지
• /
• 제34권4호
• /
• pp.249-254
• /
• 2017

The cutaneous microcirculation plays a role in various physiological processes and pathological conditions. Two non-invasive methods were used in this study to obtain reference values for cutaneous microcirculation in intact male beagles. Twenty intact male beagles were used. The experimental environments were standardized. Laser Doppler flowmetry was used to measure cutaneous blood flow, and an infrared thermometer was used to measure cutaneous temperature. The blood flow and temperature were measured from the right side of the subjects at 20 cutaneous sites. Based on the laser Doppler flowmetry, the region with the highest blood flow was the periocular region that with the lowest was the forelimb foot pad. In addition, the standard deviation of the chest wall was the highest while that of the periocular region was the lowest. For skin temperature, the inguinal region had the highest mean skin temperature and the forelimb foot pad had the lowest. The correlation coefficient between the two methods was 0.72. Similar to a previous study, the values derived from repeated measurements at the 20 regions are reproducible and can contribute to research. Compared to the results of a previous study, the temperatures of the two smallest skin regions were the same; however, no specific trend was observed. The correlation coefficient between the two methods was significantly comparable, and this good correlation can reduce their limitations and variables complementarily. In addition to possible use in human studies, accumulated resources on measurements of skin blood flow in the future will potentiate its use in the veterinary medicine field.

• 한국응용과학기술학회지
• /
• 제41권2호
• /
• pp.393-405
• /
• 2024

이 연구는 아로마 목·어깨 마사지와 Red LED 조사가 목 부위 피부 미세혈류에 미치는 영향을 조사했다. 본 연구에서는 20~60세 범위의 여성 30명을 대상으로 무작위로 분배하여 세 개의 실험그룹으로 나누었다. A 그룹 (n=10)은 아로마 목·어깨 마사지 후 Red LED 조사를 받았고 B 그룹 (n=10)은 아로마 목·어깨 마사지 후 15분 동안 휴식을 취했다. C 그룹 (n=10)은 Red LED 조사만을 받았다. 실험 결과, A 그룹과 B 그룹은 시간 요인에 대한 결과에서 목 부위 피부 미세혈류 시점 간의 주 효과가 유의함을 나타냈다 (F = 29.883, p < 0.001, 𝜂² = 0.624). 그룹 요인에 대한 통계 분석 결과 그룹 간의 주효과가 유의하지 않은 것으로 나타났으며 (F = 0.202, p = 0.659, 𝜂² = 0.011), 또한 그룹과 시간의 상호 작용이 유의했다 (F = 8.452, p = 0.001, 𝜂² =0.320). C 그룹은 실험 후 목 부위 피부 미세혈류에 통계적으로 유의한 차이가 없었다 (p > 0.05, Cohen's d = 0.202). 결론적으로, Red LED 처리가 목 피부 미세혈류를 개선하는 데에는 제한적인 효과를 나타내며 A 그룹과 B 그룹의 결과와 대조되며, 특히 A 그룹에서의 아로마 목·어깨 마사지와 Red LED 조합 처리의 효과는 유의하지 않았음을 보다 명확하게 보여준다.

• 대한미세수술학회:학술대회논문집
• /
• 대한미세수술학회 1996년도 제16차 학술대회
• /
• pp.183.1-183.1
• /
• 1996

• 한국전자파학회:학술대회논문집
• /
• 한국전자파학회 1999년도 제3회 전자장의 생체영향에 관한 워크숍
• /
• pp.117-129
• /
• 1999

Acute effects of locally applied of static magnetic field (SMF) and extremely low frequency electromagnetic field(ELF-EMF) to the cutaneous tissue within a rabbit ear chamber (REC)were evaluated under conscious conditions. Rabbits with the REC were subjected to intravital microscopical investigation by use of microphotoelectric plethysmography(MPPG). There was no dose-response relationship between the extent of vasomotion changes and frequencies(0,20,50, 100Hz)or power levels (1, 5, 10, 25, 50, 100, 200 mT). Under low vascular tone the both fields induce vasodilatation. The effects of SMF (1 mT) on the cutaneous microcirculatory system induced the vasodilatation with enhanced vasomotion under nor-adrenaline-induced high vascular tone as well as the vasoconstriction with reduced vasomotion under acetylcholine-induced low vascular tone. This suggests that the SMF can modulate vascular tone due to the modification of vasomotion biphasically in the cutaneous tissue.

• 셀메드
• /
• 제2권1호
• /
• pp.10.1-10.6
• /
• 2012

Acupuncture works by stimulating peripheral sensory nerves and their endings causing an increase in cutaneous blood flow and microcirculation, as well as release of neurotransmitters, neuropeptides, and hormones. SP6 Patch is a nanoscale nontransdermal device that mildly stimulates Stomach 36 (zusanli) and other acupuncture points. As stimulation of these points has been indicated to have an effect on hypothalamic function, it is of great research interest to investigate the effect of SP6 Patch on the physiology of organs that are affected by hypothalamic regulation. Bioelectrical tissue impedance data indicative of cellular physiologic organ function, using an Electro Interstitial Scan (EIS) system, were acquired from hypothalamus, pancreas, liver, intestines, kidneys, thyroid and adrenal glands in 10 (1 male, 9 females) volunteers while wearing the SP6 Patch daily for 1 week. EIS testing was performed at baseline with no patch, 30 min after wearing the patch, and after wearing the patch 12 h/day for 1 week. Subjects were instructed to keep well hydrated during the study period. All subjects served as their own control. The hypothesis was: The SP6 Patch worn 12 h/day on the Stomach 36 acupuncture point for 1 week, may significantly improve cellular physiologic functional status of different organs measured by EIS. All tested organs achieved significant improvement in their functional physiologic status after wearing the SP6 Pach 12 h/day for 1 week compared to baseline with an overall average statistical power > 89%. Based upon these results the hypothesis was accepted as true.

• 대한의용생체공학회:의공학회지
• /
• 제19권4호
• /
• pp.379-384
• /
• 1998

본 연구에서는 초음파를 이용하여 피부 혈관에서 그 위치에 대한 혈류 신호정보를 얻을 수 있는 펄스파(pulsed wave) 시스템을 이 분야에 응용하기 위한 가능성을 고찰하였다. 20MHz의 변환자로 수신된 신호들은 시간 영역에서 서로 비교하여 편이량을 cross-correlation방법을 사용하여 혈류속도를 구하였다. 피부의 매우 작은 혈관에서 혈류속도 거출시 발생되는 문제점에 대해 in-vitro와 in-vivo 실험을 통해서 이 방법의 효용성을 보이고 그 문제점에 대한 해결 방법을 제시한다. 시간 편이량 측정은 cross-correlation방법에 의해 정규화된 계수에서 최대점을 찾는 것이며 송신주기에 따라 반사된 수신 신호에서부터 cross-correlation방법을 사용하여 속도를 구한다. In-패패 실험을 통해 작은 튜브 내의 깊이에 대한 속도 정보를 주는 속도 profile과 이론적으로 계산된 속도 rpofile을 비교하였으며 토끼 귀 부분의 auriculares caudales에서 소동맥과 소정맥에 대한 혈류속도 profile을 구하였다. 수신 신호에서 진동에 의한 오차는 DFT를 사용하여 보정하였고 클루터 신호는 전체 수신 신호를 평균하여 하나의 기준 수신 신호를 정하여 이것에서 다른 수신 신호 값을 뺌으로써 감소시켰다.