• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.636-643
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• 2001

Bacillus sp. AIR-5, a strain from soil, produced an extracellular maltopentaose-forming amylase from amylose and soluble starch. This bacterium produced 8.9 g/l of maltopentaose from 40 g/l of soluble starch in a batch fermentation and the maltopentaose made up 90 % of the maltooligosaccharides produced (from maltose to maltoheptaose). The culture supernatant was concentrated using a 30 K molecular weight cut-off membrane and purified by DEAE-Cellulose and Sephadex G-150 column chromatographies. The purified protein showed one band on a native-PAGE and its molecular mass was estimated as 250 kDa. The 250-kDa protein was composed of tetramers of a 63-kDa protein. the isoelectric point of the purified protein was pH 6.9, and the optimum temperature for the enzyme activity was $45^{\circ}C$. The enzyme was quickly inactivated above $55^{\circ}C$, and showed a maximum activity at pH 8.5 and over 90% stability between a pH of 6 to 10. The putative N-terminal amino acid sequence of AIR-5 amylase, ATINNGTLMQYFEWYVPNDG, showed a 96% sequence similarity with that of BLA, a general liquefying amylase.

• Economic and Environmental Geology
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• v.17 no.4
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• pp.289-298
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• 1984

Uraniferous black slates of the Okchon sequence occur in Koesan (northeast) through Miwon-Boun (middle) to the southwest off Taejon (southwest) within the Okchon fold belt. The Uraniferous balck slates in the southwest off Taejon are particularly well developed in Chubu (northeast) and Moksso-ri (middle) areas whereas they are less developed in Jinsan (southwest) area. The uraniferous beds range from less than a meter to 40 meters in thickness and range from less than 0.02% $U_3O_8$ (cut-off-grade) to 0.05% $U_3O_8$ in the southwestern district off Taejon. Electron microprobe analysis of uranium-minerals found in graphitic slate samples enables to estimate their major compositions semi-quantitatively so that uraninite, ferro-uranophane and chlopinite are tentatively identified. Uranium-minerals are closely associated with carbon and metal sulfides. Correlation analysis of trace element concentrations revealed that U and F.C., and U and Mo are lineary correlative respectively and their correlation coefficients are positively high whereas those of U and V, U and Mn, and U and Zr are negatively low, implying that uranium mineralization has been closely related with concentrations of carbon and molybdenum. Stable isotope analyses of pyrite sulfur range widely from +11.5% to -23.3% in ${\delta}^{34}S$ values whereas those of graphite carbon fall within a narrow range between -23.3% and -28.9% in ${\delta}^{13}C$ values. The wide range of ${\delta}^{34}S$ values suggests that the sulfur could be of meteoric origin rather than of igneous source. The narrow range of ${\delta}^{13}C$ values, which are close to those of coal, indicates that the graphite is organic carbon in origin. Therefore, it is concluded that the uranium mineralization in the Okchon sequence took place primarily in sedimentary environment rich in organic matter and sulfide ion, both of which served as the reducing agents to convert soluble uranyl complex to insoluble uranium dioxide.

• Journal of Korean Tunnelling and Underground Space Association
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• v.13 no.3
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• pp.215-231
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• 2011

In this study, the behavior of a shallow 2-arch tunnel during the excavation in the sandy ground containing vertical discontinuity plane was experimentally studied. Load transfer mechanism in the pillar caused by a 2-arch tunnel excavation was observed. The position of the vertical discontinuity plane was varied. Model tests were carried out in the normal construction sequence of 2-arch tunnel. Test results-showed that the load transfer caused by the 2-arch tunnel excavation was concentrated in the discontinuity plane, and was cut by the discontinuity plane, so no load transfer took place above the discontinuity plane. It was also shown that the effect of adjacent tunnel excavation on the pillar load and the ground deformation was greater when excavating the upper half-face of the main tunnel, more than when excavating the lower half-face.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.3
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• pp.186-192
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• 2004

Purpose: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and Methods: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. Results: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. Conclusion: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.8A
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• pp.786-795
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• 2002

In this paper, we present a histogram and moment-based vidoe scencd change detection technique using hierarchical Hidden Markov Models(HMMs). The proposed method extracts histograms from a low-frequency subband and moments of edge components from high-frequency subbands of wavelet transformed images. Then each HMM is trained by using histogram difference and directional moment difference, respectively, extracted from manually labeled video. The video segmentation process consists of two steps. A histogram-based HMM is first used to segment the input video sequence into three categories: shot, cut, gradual scene changes. In the second stage, a moment-based HMM is used to further segment the gradual changes into a fade and a dissolve. The experimental results show that the proposed technique is more effective in partitioning video frames than the previous threshold-based methods.

• Economic and Environmental Geology
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• v.5 no.1
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• pp.1-20
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• 1972

A large anorthositic mass outcropped as mushroom-like body extending up to 46km which occurs in the Hadong kaoline district of southern Korea. The anorthositic mass is in contact with the metamorphic, plutonic and sedimentary rocks. The metamorphic rocks are of granitic gneiss and banded gneiss, etc; the plutonic rocks are of gabbroic and dioritic rocks, schistose granite, syenite, diorite and granite. The sedimentary rocks include siltstone and pebbly sandstone of Lower Gyeongsang System, Cretaceous in age. The anorthositic mass shows a gradational contact with the metamorphic and sedimentary rocks, and is cut by the plutonic rocks except gabbroic and dioritic rocks. The anorthositic mass is leucocratic in the central portion of the mass, and, in turn, grades to rock phases in which ma/ic minerals are irregularly scattered, then to the well-lineated rock and finally to the banded gneiss. Lineation of the anorthositic mass is accordant with that of the surrounding banded gneiss, and the lineation continues toward the gneiss. In some places, the rock phases in which mafics are scattered is gradational with adjacent sedimentary rocks. The anorthositic mass in contact with gabbroic and dioritic rocks shows spotted features. Various replacement features seen under the microscope and paragenetic sequence of the mineral components in the anorthositic rocks cannot be considered as the origin of magmatic crystallization. From the field and microscopic observations, it is concluded that the anorthositic mass was formed from replacement of the metamorphic rocks and plutonic rocks by the anorthositic magma.

• Parasites, Hosts and Diseases
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• v.55 no.6
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• pp.653-658
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• 2017

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

• Journal of Mechanical Science and Technology
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• v.16 no.3
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• pp.292-301
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• 2002

Ultrasound waves interact strongly with the orientation and sequence of the plies in a layup when propagating in the thickness direction of composite laminates. Also the layup orientation greatly influences its properties in a composite laminate. If the layup orientation of a ply is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. This may add a substantial cost to the production since the test is both labor intensive and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and requires less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite laminates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. High probability is found, by comparisons between the model and tests, in characterizing cured layups of the laminates by using the proposed method.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.401-406
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• 2001

When propagating the thickness direction of composite laminates ultrasound waves interacts strongly with the orientation and sequence of the plies in a layup. Also the layup orientation greatly influences its properties in a composite laminate. If one ply of the layup orientation is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. Those may add a substantial cost to the product since the test is both labor hard and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and require less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite lam mates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. It is found that a high probability shows between the model and tests developed in characterizing cured layups of the laminates.