• The Korean Journal of Zoology
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• v.37 no.2
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• pp.222-231
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• 1994

밤바구미 유충기의 whole body로부터 cyclic AMP(CAMP)를 추출하여 농도 변화를 측정하였고 cyclic AMP-dependent protein kinase(PKA)를 부분 정제하여 활성도 변화를 조사하여 CAMP 농도와 PKA 활성도와의 소장관계를 비교하였다 CAMP 농도와 PKA 활성도는 HPLC와 liquid scintillation counter를 이용하여 측정하였다 CAMP 농도는 밤바구미 유충에서 월동전에 0.57 UMlg로 가장 높았고, 월동중에 0. 14 UMIS로 감소하였다가 월동후에 0.29 UMlg로 증가하였다 또한 PKA 활성도는 월동전에 2.56unit/mg로 가장 높았으며, 월동중에 0.62 unit/mg로 감소하다가 월동후에 07 unit/mg로 다시 증가하여 CAMP 농도 변화와 유사한 경향을 나타내었다. 이는 월동전에 휴면에 대비하여 최대의 취식으로 지방체 축적이 가장 많았고, 월동중에는 지방체의 소비가 증대되.기 때문에 감소하였다가, 월동후 휴면 종결과 유충-번데기 탈피를 준비하기 위해 'CAMP 농도와 PKA 활성도는 다시 증가하였다.

• Food Science and Preservation
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• v.5 no.3
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• pp.199-206
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• 1998

Comparative effects of gamma irradiation and methyl bromide (MBr) fumigation on disinfestation and some physicochemical attributes of acorn seeds were investigated. Insects in domestic acorns were identified to be Curculio dentipes Roelofs and Dichocrocis punctiferalis Guenee, which were easily disinfestated immediately after commercial MBr fumigation. Irradiation also showed similar effects on mortality of the insects in different post-irradiation periods; at 3rd day with doses of 1-3 kGy and at around 30th day 0.5-1 kGy. MBr fumigation caused the decrease in lightness (Hunter L value), total phenolics and reducing sugars and the increase in yellowness (Hunter b value) and browning intensity for the subjected samples, as compared with the nontreated control and irradiated ones at 0.5-2 kGy. Furthermore, MBr fumigation resulted in decay for the most part of samples during storage at 5-10 $^{\circ}C$ for 6 months in a PVC box packaging with nets. As a result, optimum levels of irradiation is expected to be roe of the alternatives to chemical fumigants.

• Korean journal of applied entomology
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• v.21 no.3 s.52
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• pp.133-137
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• 1982

An experiment was conducted to find out the proper and effective fumigation method for harvested fresh chestnuts produced in Korea. Fumigation with phosphine, causing damages on chestnuts by rapid corruption or change in color on pericarp, did not completely kill chestnut curculio and nut fruit tortrix larvae in harvested fresh chestnuts regardless of long exposure of chestnuts to phosphine. Any damages not being appeared by fumigant, fumigation with methyl bromide at $40g/m^3$ for 4 hours at $21^{\circ}C$ would retain the low control against inclusion of any chestnut pests tested. Though fumigation with methyl bromide at $50g/m^3$ for 24hours was effective against any larvae of chestnut insects control, they caused serious damages by making the flesh surface of harvested fresh chests dark or dense brown. However, fumigation with methyl bromide at $50g/m^3$ for 4 hours at $21^{\circ}C$ resulted in complete kill of larvae of Curculio dentipes and Carpocapsa splendana in harvested fresh chestnuts with no significant damages on the treated fruits.