• 제목/요약/키워드: Cultured-Root

검색결과 576건 처리시간 0.023초

수종 근관충전세멘트의 Streptococcus Sanguis에 대한 항균효과에 관한 실험적 연구 (ANTIMICROBIAL EFFECT OF ROOT CANAL CEMENTS ON STREPTOCOCCUS SANGUIS)

  • 권오양;윤수한
    • Restorative Dentistry and Endodontics
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    • 제12권1호
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    • pp.77-84
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    • 1986
  • This study was designed to compare the antimicrobial effect of the several root canal cements and to determine the duration of their activity. After Strep. sanguis 9811 was streaked on the surface of BHI agar and Plain agar, PVC tubes filled with root canal cements were applied and cultured for 48 hours, aerobically. Following results were obtained, 1. All of the examined root canal cements had antimicrobial activity with varying degree. 2. The antimicrobial activity of N2 was larger than any other root canal cements. Nogenol and Tubliseal showed the lowest activity. ZOE, AH26, Procosol and PCA cement showed the moderate antimicrobial activity. (P>0.05) 3. At one day after mixing the root canal cements, all of the root canal cements except N2 and PCA cement showed greatly reduced antimicrobial activity. 4. At three days after mixing, PCA cement showed the reduced antimicrobial activity. N2 showed the reduced activity at 7 days after mixing.

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제주상사화 (Lycoris chejuensis K. Tae et S. Ko) 잎 및 뿌리 절편으로부터 소자구 형성을 통한 식물체 재생안 (Plant Regeneration from Leaf and Root Cultures of Lycoris chejuensis via Bulblet Formation)

  • 오명진;박종미;태경환;유장렬;김석원
    • Journal of Plant Biotechnology
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    • 제34권3호
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    • pp.223-227
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    • 2007
  • 제주상사화의 잎 및 뿌리 조직으로부터 형성된 캘러스 및 구형 소자구로부터 효율적인 기내 식물체 재생체계를 확립하였다. 2,4-D가 첨가된 B5 배양배지에서 12주 배양 후 제주상사화의 잎 조직으로부터 백색의 구형 세포괴 및 캘러스가 동시에 발달하였으며 그 빈도는 32.1%이었다. 그러나 3 mg/L 이상의 고농도 2,4-D 처리구에서는 캘러스 형성빈도가 14%로 크게 감소하였으며 10 mg/L 2,4-D 처리구에서는 캘러스 형성이 전혀 이루어지지 않았다. 잎 조직과 달리 뿌리 절편의 경우 3 mg/L 2,4-D 처리구에서 캘러스 형성빈도가 36.1%로 가장 높았으며 BA 단독처리구나 2,4-D와 BA의 혼용처리구에서는 그 빈도가 감소하였다. 형성된 백색의 구형세포괴는 생장조절제가 첨가되지 않은 B5배지에 배양하면 소자구 발달 경로를 거쳐 소식물체로 발달이 이루어졌다. 소식물체는 생장조절제가 첨가되지 않은 1/2MS 기본배지로 옮겨 명배양한 결과, 약 배양 2주후부터 녹색의 잎이 신장되는 것을 관찰 하였으며 4주 후 뿌리 발달이 이루어지면서 정상적인 식물체로 발달하였다. 재생된 소식물체는 배양기내에서 순화과정을 통해 토양이식이 가능하였다. 본 연구에서 확립된 제주상사화의 식물체 재생체계는 제주상사화의 대량증식 수단은 물론, 유용 형질 도입을 통한 분자육종의 수단 및 유용 유전자원의 장기보전을 위한 초저온 보존 연구의 직접적인 연구소재로 활용이 가능할 것으로 예상된다.

직파와 이식재배에 따른 인삼의 생육특성 및 엑스와 조사포닌 함량 (Comparison of Growth Characteristics, and Extract and Crude Saponin Contents in 4- Year-Old Ginseng Cultured by Direct Seeding and Transplanting Cultivation)

  • 이성우;차선우;현동윤;김영창;강승원;성낙술
    • 한국약용작물학회지
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    • 제13권6호
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    • pp.241-244
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    • 2005
  • 금산 및 음성지역의 4년생 인삼재배 농가포장에서 직파재배 5개소와 이식재배 5개소를 임의로 선정하여 직파와 이식재배에 따른 생육특성 및 엑스와 조사포닌 함량을 비교한 결과는 다음과 같다. 직파재배는 4년근 생존율이 평균 48%로 이식재배의 86%보다 떨어지나 입모수가 평균 96주/3.3$m^2$순로 이식재배의 57주보다 많고 엽면적지수가 커 수량성이 높은 반면, 주당근중은 작았다. 직파재배는 이식재배에 비해 동체의 신장이 양호하나 지근의 발달이 불량하여 동체중의 비율이 높고 지근중의 비율이 낮았으며, 직파재배는 적변 발생율이 적으나 동체와 지근부위의 엑스와 조사포닌 함량이 낮았다.

오옥신 처리가 에키네시아의 부정근 및 생리활성물질 생산에 미치는 영향 (Auxin Affects on Production of Adventitious Roots and Secondary Metabolites in Echinacea angustifolia)

  • 장영세;최해연;이은정;김해원;백기엽
    • 한국약용작물학회지
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    • 제20권6호
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    • pp.479-486
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    • 2012
  • The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.

In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

  • Lim, Jong-Gu;Junzo, Fujigaki
    • Plant Resources
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    • 제7권3호
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    • pp.222-225
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    • 2004
  • In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

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Effect of Rhizoma gastrodiae on glucose oxydase induced neurotoxicity in cultured mouse spinal dorsal root ganglion neurons

  • Park, Seung-Taeck;Park, Yang-Kyu;Park, Jae-Hwang;Cho, Kwang-Ho;Ryu, Do-Gon;Jeon, Byung-Hoon;Shin, Min-Kyo;Han, Du-Seok;Cho, Nam-Su;Shin, Dong-Min
    • Advances in Traditional Medicine
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    • 제1권1호
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    • pp.64-70
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    • 2000
  • Effects of Rhizoma gastrodiae on glucose oxidase-induced neurotoxicity was investigated in cultured newborn mouse spinal dorsal root ganglion(DRG) neurons that were treated in the media with or without glucose oxidase. In addition, the protective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity was examined. Cytotoxic values were expressed as a percentage of number of living cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In this paper, exposure of neurons to glucose oxidase resulted in a significant call death in a dose- and time-dependent manners in DRG neuron cultures. The decrease in cell viability induced by the glucose oxidase was blocked by Rhizoma gastrodiae extract. These results indicate that the neuroprotective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity may result from a prevention or attenuation of oxidative damage induced by glucose oxidase.

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Effect of Antimitotic Agent Colchicine on In Vitro Regeneration of Watermelon

  • Jaskani Muhammad J.;Raza H.;Khan M. M.;Kwon Sung W.
    • Journal of Plant Biotechnology
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    • 제6권4호
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    • pp.247-252
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    • 2004
  • In vitro cultures of watermelon were treated with antimitotic agent colchicine to induce ploidy alterations, particularly the induction of tetraploids. Explants cotyledon, embryonic end of seed, transverse sections of epicotyl and hypocotyl were cultured on MS media supplemented with BA ($1{\mu}M$) and colchicine ($0.01\%,\;0.05\%\;and\;0.1\%$). Explants were subcultured on colchicine free media after 4 and 7 days. Colchicine had negative effect on in vitro regeneration but this exhibited explants related response. However, hypocotyl section of seedlings induced maximum callus on $0.01\%$ colchicine. Shoot proliferation was more in cotyledon explants cultured on colchicine ($0.01\%$) for four days. Maximum root induction and root number were recorded in embryonic end explants. Overall, cotyledon and embryonic end explants, and low colchicine concentration ($0.01\%$) was found optimal in watermelon regeneration.

Pigment and Saikosoponin Production Through Bioreactor Culture of Carthamus tinctorius and Bupleurum falcatum

  • Wenyuan Gao;Lei Fan;Hahn, Eun-Joo;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • 제3권1호
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    • pp.1-5
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    • 2001
  • Traditional culture technology of medicinal plants mainly depends on the field culture, which has many problems. With progress of modern culture technology, it has become possible to produce valuable secondary metabolites from medicinal plants. In this paper, we discuss about the pigment and saikosaponin production from too medicinal plants, Carthamus tinctorius and Bupleurum falcatum, through bioreactor culture system. A two-stage bioreactor culture system was established for the production of yellow and red pigments and saikosaponins by cell suspension cultures of Carthamus tinctorius and Bupleurum falcatum. In Carthamus tinctorius, balloon type airlift bioreactors and column type airlift bioreactors were employed for the tell culture and for the pigment production, respectively. The greatest pigment production was obtained on White medium supplemented with 4 mg/L kinetin, high levels of sucrose concentration and photosynthetic photon flux. In Bupleurum falcatum, adventitious roots were cultured in balloon type airlift bioreactors and the root growth was greatest on SH medium containing 5 mg/L IBA and 0.2 mg/L kinetin. HPLC analysis showed that the contents of main active saikosaponins a, c, and d in adventitious roots were almost the same as those in field cultured root.

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Identification of Differentially Expressed Genes by Gabapentin in Cultured Dorsal Root Ganglion in a Rat Neuropathic Pain Model

  • Heo, Ji Hye;Lee, Seung Ha;Chang, Kyung Ha;Han, Eun Hye;Lee, Seung Gwan;Choi, Dal Woong;Kim, Suhng Wook
    • Biomolecules & Therapeutics
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    • 제21권2호
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    • pp.126-131
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    • 2013
  • Neuropathic pain is a chronic pain disorder caused by nervous system lesions as a direct consequence of a lesion or by disease of the portions of the nervous system that normally signal pain. The spinal nerve ligation (SNL) model in rats that reflect some components of clinical pain have played a crucial role in the understanding of neuropathic pain. To investigate the direct effects of gabapentin on differential gene expression in cultured dorsal root ganglion (DRG) cells of SNL model rats, we performed a differential display reverse transcription-polymerase chain reaction analysis with random priming approach using annealing control primer. Genes encoding metallothionein 1a, transforming growth factor-${\beta}1$ and palmitoyl-protein thioesterase-2 were up-regulated in gabapentin-treated DRG cells of SNL model rats. The functional roles of these differentially expressed genes were previously suggested as neuroprotective genes. Further study of these genes is expected to reveal potential targets of gabapentin.

Activation of the cGMP/Protein Kinase G Pathway by Nitric Oxide Can Decrease TRPV1 Activity in Cultured Rat Dorsal Root Ganglion Neurons

  • Jin, Yun-Ju;Kim, Jun;Kwak, Ji-Yeon
    • The Korean Journal of Physiology and Pharmacology
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    • 제16권3호
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    • pp.211-217
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    • 2012
  • Recent studies have demonstrated that nitric oxide (NO) activates transient receptor potential vanilloid subtype 1 (TRPV1) via S-nitrosylation of the channel protein. NO also modulates various cellular functions via activation of the soluble guanylyl cyclase (sGC)/protein kinase G (PKG) pathway and the direct modification of proteins. Thus, in the present study, we investigated whether NO could indirectly modulate the activity of TRPV1 via a cGMP/PKG-dependent pathway in cultured rat dorsal root ganglion (DRG) neurons. NO donors, sodium nitroprusside (SNP) and S-nitro-N-acetylpenicillamine (SNAP), decreased capsaicin-evoked currents ($I_{cap}$). NO scavengers, hemoglobin and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CPTIO), prevented the inhibitory effect of SNP on $I_{cap}$. Membrane-permeable cGMP analogs, 8-bromoguanosine 3', 5'-cyclic monophosphate (8bromo-cGMP) and 8-(4chlorophenylthio)-guanosine 3',5'-cyclic monophosphate (8-pCPT-cGMP), and the guanylyl cyclase stimulator YC-1 mimicked the effect of SNP on $I_{cap}$. The PKG inhibitor KT5823 prevented the inhibition of $I_{cap}$ by SNP. These results suggest that NO can downregulate the function of TRPV1 through activation of the cGMP/PKG pathway in peripheral sensory neurons.