• 제목/요약/키워드: Culture-independent method

검색결과 129건 처리시간 0.023초

인문지리학 방법론의 새로운 지평 (New horizon of geographical method)

  • 최병두
    • 대한지리학회지
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    • 제38권
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    • pp.15-36
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    • 1988
  • In this paper, I consider the development of methods in contemporary human geography in terms of a dialectical relation of action and structure, and try to draw a new horizon of method toward which geographical research and spatial theory would develop. The positivist geography which was dominent during 1960s has been faced both with serious internal reflections and strong external criticisms in the 1970s. The internal reflections that pointed out its ignorance of spatial behavior of decision-makers and its simplication of complex spatial relations have developed behavioural geography and systems-theoretical approach. Yet this kinds of alternatives have still standed on the positivist, geography, even though they have seemed to be more real and complicate than the previous one, The external criticisms that have argued against the positivist method as phenomenalism and instrumentalism suggest some alternatives: humanistic geography which emphasizes intention and action of human subject and meaning-understanding, and structuralist geography which stresses on social structure as a totality which would produce spatial phenomena, and a theoretical formulation. Human geography today can be characterized by a strain and conflict between these methods, and hence rezuires a synthetic integration between them. Philosophy and social theory in general are in the same in which theories of action and structural analysis have been complementary or conflict with each other. Human geography has fallen into a further problematic with the introduction of a method based on so-called political ecnomy. This method has been suggested not merely as analternative to the positivist geography, but also as a theoretical foundation for critical analysis of space. The political economy of space with has analyzed the capitalist space and tried to theorize its transformation may be seen either as following humanistic(or Hegelian) Marxism, such as represented in Lefebvre's work, or as following structuralist Marxism, such as developed in Castelles's or Harvey's work. The spatial theory following humanistic Marxism has argued for a dialectic relation between 'the spatial' and 'the social', and given more attention to practicing human agents than to explaining social structures. on the contray, that based on structuralist Marxism has argued for social structures producing spatial phenomena, and focused on theorising the totality of structures, Even though these two perspectives tend more recently to be convergent in a way that structuralist-Marxist. geographers relate the domain of economic and political structures with that of action in their studies of urban culture and experience under capitalism, the political ecnomy of space needs an integrated method with which one can overcome difficulties of orthhodox Marxism. Some novel works in philosophy and social theory have been developed since the end of 1970s which have oriented towards an integrated method relating a series of concepts of action and structure, and reconstructing historical materialism. They include Giddens's theory of structuration, foucault's geneological analysis of power-knowledge, and Habermas's theory of communicative action. Ther are, of course, some fundamental differences between these works. Giddens develops a theory which relates explicitly the domain of action and that of structure in terms of what he calls the 'duality of structure', and wants to bring time-space relations into the core of social theory. Foucault writes a history in which strategically intentional but nonsubjective power relations have emerged and operated by virtue of multiple forms of constrainst wihthin specific spaces, while refusing to elaborate any theory which would underlie a political rationalization. Habermas analyzes how the Western rationalization of ecnomic and political systems has colonized the lifeworld in which we communicate each other, and wants to formulate a new normative foundation for critical theory of society which highlights communicatie reason (without any consideration of spatial concepts). On the basis of the above consideration, this paper draws a new norizon of method in human geography and spatial theory, some essential ideas of which can be summarized as follows: (1) the concept of space especially in terms of its relation to sociery. Space is not an ontological entity whch is independent of society and has its own laws of constitution and transformation, but it can be produced and reproduced only by virtue of its relation to society. Yet space is not merlely a material product of society, but also a place and medium in and through which socety can be maintained or transformed.(2) the constitution of space in terms of the relation between action and structure. Spatial actors who are always knowledgeable under conditions of socio-spatial structure produce and reproduce their context of action, that is, structure; and spatial structures as results of human action enable as well as constrain it. Spatial actions can be distinguished between instrumental-strategicaction oriented to success and communicative action oriented to understanding, which (re)produce respectively two different spheres of spatial structure in different ways: the material structure of economic and political systems-space in an unknowledged and unitended way, and the symbolic structure of social and cultural life-space in an acknowledged and intended way. (3) the capitalist space in terms of its rationalization. The ideal development of space would balance the rationalizations of system space and life-space in a way that system space providers material conditions for the maintainance of the life-space, and the life-space for its further development. But the development of capitalist space in reality is paradoxical and hence crisis-ridden. The economic and poltical system-space, propelled with the steering media like money, and power, has outstriped the significance of communicative action, and colonized the life-space. That is, we no longer live in a space mediated communicative action, but one created for and by money and power. But no matter how seriously our everyday life-space has been monetalrized and bureaucratised, here lies nevertheless the practical potential which would rehabilitate the meaning of space, the meaning of our life on the Earth.

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텍스트마이닝 기법을 통한 직업상담서비스산업의 직무 및 지식구조 분석 (Textmining-Based Analysis for Duty and Knowledge structure of Vocational Counseling Service Industry)

  • 육동인
    • 한국산학기술학회논문지
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    • 제18권9호
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    • pp.366-376
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    • 2017
  • 본 논문은 빅데이터 분석방법인 텍스트마이닝 기법과 네트워크분석을 활용하여 직업상담서비스 산업의 직무와 지식구조를 분석하기 위한 것이다. 정부에서 추진하는 국가직무능력표준(NCS)에서 제시한 직업상담, 전직지원, 취업알선 세 분야의 능력단위요소를 조사 대상으로 하였다. 사용빈도수가 높았던 단어들에 대한 분석결과 이 세 분야는 각각의 독자적인 직무도 있었지만 내담자, 구직자 등을 대상으로 상담, 검사, 취업지원 등 중복된 직무가 많았다. 직업상담서비스 분야 종사자들에게 요구되는 지식은 직업상담사 자격시험에 포함되어 있는 직업심리학, 직업상담학, 노동시장론, 직업정보론, 법률 등 5가지 외에도 창업, 생애진로, 직업적응, 문화충돌 등 새로운 분야가 다수 포함되어 있었다. 또한 상담자들에게는 공감, 수용, 경청 등 감성적인 접근과 함께 객관적, 가치중립, 정확 등의 태도를 중시하는 등 가치중립적인 입장을 견지할 것을 제시했다. 본 논문은 직업상담서비스분야의 직무를 빅데이터 분석을 통해 객관적으로 분석한 첫 시도라는 점에서 의의가 있다. 또한 정부의 정책방향과 실제 산업 현장의 차이를 처음으로 구체적으로 확인했다는 점도 의미있다고 할 수 있다.

Karasek의 Job Content Questionnaire와 Korea Occupational Stress Scale의 비교 연구 (Comparison of Karasek's Job Content Questionnaire and Korea Occupational Stress Scale)

  • 이종빈;장성록
    • 한국안전학회지
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    • 제30권1호
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    • pp.72-78
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    • 2015
  • According to the report of the WHO, workers have been exposed to much job strain such as job load, responsibility, role, interpersonal conflict etc. In Korea, studies on job strain started to become active from 1990s and now hundreds of studies are actively under going or publishing so that the studies are contributing to development and improvement of job strain. Representative measurement models of job strain are Job Strain Model of Karasek, Job Stress Model of NOISH, Korea Occupational Stress Scale, JSQ(Job Stress Questionnaire), K-OSI(Korea Version of Occupational Stress Inventory) etc. (Lee Kwan-Suk, 2012 ; KOSHA, 2003). Among them, Job Strain Model of Karasek had been loved by many researchers of job strain before Korea Occupational Stress Scale was developed. Job Strain Model of Karasek had been fitted to Korean style and then, used to analyze job strain of Korean people so that this Scale highly contributed to seeking relationship with cardiovascular disease, musculoskeletal disease caused by job, smoking, drug, alcohol poisoning, and pulse(Lee Kwan-Suk, 2012). But as this Model was studied and developed based on foreign culture and life pattern, a model fit to Korea was developed to measure job strain for Korean people, which is Korea Occupational Stress Scale now most frequently used in measuring job strain. Accordingly, after this study made questionnaire survey about same population using the two methods used most frequently in measuring job strain, the study investigated what features appeared, what correlations appear between two models, and comparatively analyzed characteristics each independent and dependent variable. Based on this, the study aimed to exactly express job strain of Korean people. The subjects of the study were a population of 233, and Karasek's Questionnaire and KOSS's Questionnaire were surveyed at the same time. The results were analyzed by statistical program to obtain significant difference between two models. Four particular groups were divided with Job Strain Model of Karasek and the four particular groups were measured with Korea Occupational Stress Scale. And job strain come from combination of two models was measured, with which new comparative analysis method was suggested.

중국 경극 가면을 응용한 패치워크 기법의 의상 디자인 연구 (Study on Chinese Opera Masks Applied Fashion Design -Based on a Patchwork Technique-)

  • 이설매;이상희;한설아
    • 한국의류학회지
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    • 제36권2호
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    • pp.194-205
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    • 2012
  • Historically, garments have been spontaneously made with human instinct in order to warm and decorate the body using textiles that have been made and used by various peoples in different cultures. Contemporary garments have been used as a means of warming as well as express individual personality and a desire to pursue beauty. They have a unique formativeness and a symbolic nature according to cultural phenomena that reflect a cultural outcome that expresses contemporary thinking and ideology as well as their aesthetic consciousness. This study globally promotes Chinese opera mask culture and proposes the possibility of creative thinking through the introduction of Chinese opera mask elements into contemporary fashion, expressing them as patchworks. In order to endow artistic value (required in contemporary fashion) this study creates works by analyzing and applying a formative sense seen in patchwork techniques and the characteristics of Chinese opera masks; in addition, it sought the possibility of new expressions in garment designs. The results obtained from the research involve the following. First, the introduction of the traditional patchwork technique to contemporary garments may be a dynamic theme of garment expression and exceed stereotypical ideas that enable the creation of designs attuned to the globalization era. Second, it was possible to pursue independent formativeness which is in harmony with a modern sense through the restructuring of the harmony of colors and practical characteristics found in patchworks. Third, it was possible to demonstrate their artistry and unique effect in the expression of contemporary garments by the expression of the analyzed results of characteristic patterns of Chinese opera masks with a patchwork technique in creating works. Results show that it is necessary to conduct research into a patchwork technique that applies diverse materials as a new method to develop contemporary garment design also as a creative design. In addition, if the analyzed results of the characteristic patterns of Chinese opera masks can be expressed with a patchwork technique, they may be able to exhibit a genuine effect along with the artistry of expression in contemporary garments; thereby, relevant follow-up research should be further continued.

당근의 체세포배로부터 발생한 이상자엽 유식물의 유관속 조직의 구조적 특성 (Structural Characteristics of Vascular Tissue in Carrot Seedlings with Anomalous Cotyledon Developed from Somatic Embryos)

  • 홍성식;소웅영
    • 식물조직배양학회지
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    • 제25권1호
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    • pp.69-74
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    • 1998
  • 당근(Daucus carota L cv. Hongshim)의 체세포배를 MS 기본배지에서 4주간 현탁배양하여 얻어진 유식물들은 자엽수에 따라 유형별로 구별되었고, paraffin method에 의한 영구표본을 제작하여 유관속 구조를 관찰하였다. 자엽의 엽육은 책상조직과 해면조직으로 뚜렷한 분화가 되어 있지 않았다. 모든 유형의 자엽에서 유관속은 독립된 병립유관속으로 배열되어 있지 않고, 서로 연결되어 있거나 부분적으로 연결되어 있다. 자엽기부는 대부분의 유형에서 짧은 원통상 구조를 보인다. 자엽기부와 자엽절에서 유관속의 배열은 주발형자엽 및 1자엽배에서는 환상으로 연결되므로서 외사관상중심주의 형태를 보이는 경우가 많고, 5자엽배에서도 가끔 관찰되었다. 2자엽, 3자엽 및 4자엽배의 자엽기부와 자엽절에서는 주로 독립적으로 배열된 진정중심주가 관찰되었다. 배축에서 유관속의 배열은 주발형자엽, 1자엽 및 5자엽배에서는 4-6원형 목부가, 2자엽배에서는 4원형 목부가 많고, 3자엽배에서는 3원형 목부가 많았다. 자엽절-배축에는 대부분의 경우에 피층유관속이 산재하며, 드물게 이중유관속도 나타난다. 2자엽배에서는 대부분이 2원형 목부이며, 3자엽배에서는 3원형 목부가 절반정도 관찰되며, 나머지에서도 주로 2원형 목부이지만 드물게 4원형 목부도 관찰되었다.

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Genomic and Proteomic Analysis of Microbial Function in the Gastrointestinal Tract of Ruminants - Review -

  • White, Bryan A.;Morrison, Mark
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권6호
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    • pp.880-884
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    • 2001
  • Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

Elevated Platelets Enhance Cancer Cell Migration, Promote Hematogenous Metastasis and Associate with a Poor Prognosis in Advanced Non-small Cell Lung Cancer Cases

  • Li, Yan;Miao, Li-Yun;Xiao, Yong-Long;Cai, Hou-Rong;Zhang, De-Ping
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권1호
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    • pp.139-143
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    • 2014
  • Although correlations between platelets and lung cancer has been recognized, effects on non-small cell lung cancer (NSCLC) metastasis remain to be determined in detail. In the present study, wound healing assays revealed a role of platelets in NSCLC cell migration. Thus the mean migration rate of lung adenocarcinoma A549 cells was significantly elevated after co-culture with platelets ($81.7{\pm}0.45%$ vs $41.0{\pm}3.50%$, P<0.01). Expression of GAPDH was examined by reverse transcription-polymerase chain reaction to study the effect of platelets on NSCLC cell proliferation. The result showed that the proliferation of A549 and SPC-A1 cells was not affected. Mouse models were established by transfusing A549 cells and SPC-A1 cells into mice lateral tail veins. We found tumor metastasis nodules in lungs to be increased significantly after co-transfusion with platelets (in A549, $4.33{\pm}0.33$ vs $0.33{\pm}0.33$, P=0.01; in SPC-A1, $2.67{\pm}0.33$ vs $0.00{\pm}0.00$, P=0.01). In addition, consecutive inoperable patients with newly diagnosed NSCLC (TNM stage III or IV) between January 2009 and December 2011 were retrospectively reviewed. Using the Kaplan-Meier method, NSCLC patients with a high platelet counts demonstrated a significantly shorter progression free survival compared with those with a low platelet count (> $200{\times}10^9/L$, 3 months versus ${\leq}200{\times}10^9/L$, 5 months, P=0.001). An elevated platelet count was also identified as an independent prognostic factor by Cox regression analysis for prgression free survival (adjusted hazard ratio: 1.69; 95% CI: 1.16, 2.46; P=0.006). This study suggested that platelets might contribute to the hematogenous metastatic process by promoting cancer cell migration, which eventually affects the prognosis of NSCLC.

Hypoxia Induced High Expression of Thioredoxin Interacting Protein (TXNIP) in Non-small Cell Lung Cancer and its Prognostic Effect

  • Li, Yan;Miao, Li-Yun;Xiao, Yong-Long;Huang, Mei;Yu, Min;Meng, Kui;Cai, Hou-Rong
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권7호
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    • pp.2953-2958
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    • 2015
  • Although associations between thioredoxin interacting protein (TXNIP) and cancers have been recognized, the effects of TXNIP on non-small cell lung cancer (NSCLC) prognosis remained to be determined in detail. In addition, while hypoxia is a key characteristic of tumor cell growth microenvironment, the effect of hypoxia on TXNIP expression is controversial. In this study, formaldehyde fixed and paraffin embedded (FFPE) samples of 70 NSCLC patients who underwent resection between January 2010 and December 2011 were obtained. Evaluation of TXNIP and hypoxia inducible factor-$1{\alpha}$ ($HIF-1{\alpha}$) protein expression in FFPE samples was made by immunohistochemistry. By Kaplan-Meier method, patients with high TXNIP expression demonstrated a significantly shorter progression free survival (PFS) compared with those with low TXNIP expression (18.0 months, 95%CI: 11.7, 24.3 versus 23.0 months, 95%CI: 17.6, 28.4, P=0.02). High TXNIP expression level was also identified as an independent prognostic factor by Cox regression analysis (adjusted hazard ratio: 2.46; 95%CI: 1.08, 5.56; P=0.03). Furthermore, TXNIP expression was found to be significantly correlated with $HIF-1{\alpha}$ expression (Spearman correlation=0.67, P=0.000). To further confirm correlations, we established a tumor cell hypoxic culture model. Expression of TXNIP was up-regulated in all three NSCLC cell lines (A549, SPC-A1, and H1299) under hypoxic conditions. This study suggests that hypoxia induces increased TXNIP expression in NSCLC and high TXNIP expression could be a poor prognostic marker.

맥파 스트레스와 한국인 직무스트레스의 상관관계 분석 - 도시철도 건설종사자를 대상으로 (Analysis of the Relationship between Macpa Stress Index and Korean Job Stress Level - Focusing on Subway Construction Workers)

  • 채정식;이유정;장성록
    • 한국안전학회지
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    • 제37권1호
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    • pp.64-69
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    • 2022
  • The study measured a subway construction worker's Macpa stress by Heart Rate Variability measuring instrument and conducted a survey of Korean job stress from subway construction workers. Also, the study analyzed the relationship between Macpa stress index and Korean job stress result and suggested managing stress method for each item. According to National Statistical Office data, the first line subway in Seoul was started to open in 1974. The extended total length is 996 kilometers until 2019. Many aged workers are currently working at subway construction sites due to the avoidance of young workers since the past until now. It means that the elderly has a substantial portion among subway construction workers. The productivity has been adversely affected by health problems due to the aging of workers, job stress due to heavy work, and personal health problems. So, the regulation and policies on job stress health management are being strengthened. The data were measured Macpa stress by machine measuring heart rate variability and conducted Korean job stress survey(shortened) from Sa-sang to Ha-dan line Busan subway construction workers for analyzing the relationship. Independent variable were age, job duration, job position, employment type, working type in this study. Macpa's dependent variable was stress index and Korean job stress survey(shortened)'s dependent variables were job requirements, job autonomy, relationship conflict, job instability, organizational structure, inappropriate compensation, working place culture, and total score. SPSS 12.0 K Statistics Program was used for statistical analysis. Kruskal-wallis test, a nonparametric statistical analysis, was used because the data are difficult to be assumed as normal distribution. As a result, the paper indicated the significant correlation between Macpa stress index and Korean job stress(short version). The elderly workers presented higher Macpa index and higher job stress due to aging and heavy-duty work. The majority workers were daily workers who had unstable working condition and uncertainty about the future. The study suggested a manual that could reduce job stress for subway construction workers and future study deriving management tool through analyzing job stress factor is necessary.

유튜브 먹방과 온라인 배달 주문: 영향력 분석과 예측 모형 (Youtube Mukbang and Online Delivery Orders: Analysis of Impacts and Predictive Model)

  • 최사라;이상용
    • 지능정보연구
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    • 제28권4호
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    • pp.119-133
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    • 2022
  • 음식 문화 및 산업과 관련한 대표적 특징들 중에는 음식 배달 주문 산업이 성장하고 있다는 것과 유튜브와 같은 1인 미디어에서의 소위 '먹는 방송' (먹방)이 최고의 인기 콘텐츠로 자리 잡았다는 사실 등을 거론할 수 있다. 본 연구는 이러한 배경에 근거하여 두가지 초점을 두어 연구하고자 하였다. 먼저, 유튜브 먹방과 먹방 댓글에서 확인되는 대중들의 감성이 관련 음식의 배달 이용 건수에 영향을 미치는지를 회귀분석 모형을 통하여 확인하고자 하였다. 다음으로, 대한민국에서 대표적인 주문 음식인 치킨의 배달 이용 건수 데이터와 유튜브 먹방 댓글 데이터와 날씨 데이터를 활용하여, 머신 러닝을 통한 치킨 배달 주문 예측 모형을 구현하였다. 2015년 6월 3일부터 2019년 9월 30일까지 총 1,580개의 데이터를 활용하였고, 날씨 변수로서의 온도, 습도, 강수량과 유튜브 먹방 변수로의 영상에 달린 댓글 수, 댓글의 긍정어 수, 중립어 수, 부정어 수 등을 수집하였다. 본 연구에 활용된 데이터의 유튜브 먹방과 먹방 댓글의 감성이 배달 이용 건수에 영향 미침을 확인하기위해 선형 회귀 방법론을 사용하였으며, 예측모델을 위해 사용된 머신 러닝은 Linear Regression, Ridge, Lasso, Random Forest, Gradient Boost이다. 본 연구를 통해 유튜브 먹방과 댓글의 감성이 배달 이용 건수에 영향 미침을 확인하였고 예측 모형 또한 기존 모델보다 성능이 좋아짐을 Root Mean Square Error 값을 통하여 확인하였다. 본 연구는 먹방의 광고 효과를 확인하였으며, 배달 업종에서의 경영에 활용할 수 있는 함의를 제공하고자 하였다.