• 제목/요약/키워드: Culture collection

검색결과 895건 처리시간 0.028초

지역경관자원을 활용한 제주상효식물원 계획 (Master Plan for Jeju Sanghyo Botanical Garden using local landscape resources)

  • 박은영;유병림
    • 농촌계획
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    • 제15권4호
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    • pp.173-182
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    • 2009
  • This study is a plan for the Jeju Sanghyo Botanical Garden construction, and it is prepared to maintain the identity of the local landscape by composing the garden using the local landscape resources. The goals of becoming one of world's best, garden centered botanical garden and at the same time one that represents culture of Jeju. Unusual species are included in the collection and with the general collection, special collection and exhibition by different kinds are planned to be offered to the public. The garden will demonstrate the world's various types of gardens in a compressed way. It is our endeavor to become center of culture beyond plant education and exhibition, drawing a large number of people to our garden. Moreover, conservation of Jeju's unique botanic resources will be carried out and the garden will show the garden culture. Due to the size of the site, different themes will be exhibited in one zone in a parallel. Unique combination of each local landscape resource will create own theme. The display will be divided into three different stages: the 1st stage is an educational exhibition, assisting more understanding of distinguishing different locations, the second is exhibition by various species and the final stage will show an ideal way to develop a garden with harmony of various plant species.

Aspergillus cumulatus sp. nov., from Rice Straw and Air for Meju Fermentation

  • Kim, Dae-Ho;Kim, Seon-Hwa;Kwon, Soon-Wo;Lee, Jong-Kyu;Hong, Seung-Beom
    • Journal of Microbiology and Biotechnology
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    • 제24권3호
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    • pp.334-336
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    • 2014
  • A new species named Aspergillus cumulatus sp. nov. is described in Aspergillus section Aspergillus (Eurotium state). The type strain (KACC $47316^T$) of this species was isolated from rice straw used in meju fermentations in Korea, and other strains were isolated from the air in a meju fermentation room. The species is characterized by growth at a wide range of water activities and the formation of aerial hyphae on malt extract 60% sucrose agar (ME60S) that resemble a cumulus cloud. Furthermore, A. cumulatus produces yellow ascomata containing small lenticular ascospores (5.1-5.7 ${\mu}m$) with a wide furrow, low equatorial crests, and tuberculate convex surface. The species is phylogenetically distinct from the other reported Aspergillus section Aspergillus species based on multilocus sequence typing using rDNA-ITS, ${\beta}$-tubulin, calmodulin, and RNA polymerase II genes.

냉장 저장 중인 청도반시에 발생한 곰팡이의 동정 (Identification of Fungi Isolated from Cheongdo-Banshi (flat persimmon) Stored in a Refrigerator)

  • 방나래;하상오;김대호;김선화;홍승범
    • 한국균학회지
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    • 제42권4호
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    • pp.276-281
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    • 2014
  • 냉장 저장 중에 있는 청도반시로부터 서로 다른 모양을 나타내는 곰팡이 7 균주를 분리하였다. 분리한 곰팡이를 형태적 특성과 분자적 특성에 근거하여 동정한 결과 이들은 Botrytis cinerea Pers. (4 균주), Penicillium expansum Link (2 균주), Rhizopus delemar (Boidin) Wehmer & Han-zawa (1 균주)로 동정되었다. 이들 중에서는 B. cinerea와 P. expansum이 빈번하게 관찰되었고 R. delemar는 낮은 빈도로 발생하였다. P. expansum과 R. delamar는 국내의 감에서 아직 보고되지 않은 종이기에 본고에서 보고하고자 한다.

1990년대의 히피패션에 관한 연구 (A Study on Happie Fashion in 1990′s)

  • 한미경;은영자
    • 복식문화연구
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    • 제9권2호
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    • pp.188-206
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    • 2001
  • In 1990's, many collections, showing trends of restoration, took the hippie fashion as their main theme. Therefore, this study investigates the aspects of the hippie trend focusing on style, materials, colors shown in Mlan Collection, New York Collection, London Collection, Paris Collection, Tokyo Woman's Collection, and street fashions. 1. In style, the collections and street fashions exhibited layered look. 2. In materials, natural fibers were preferred s in 1960's . 3. In colors, natural hues such as black, white, and brown were primarily used, whereas vivid colors were used as accent. 4. In patterns, floral patterns were most extensively used as the flower is the symbol of Hippie. Animal patterns were also used. 5. In accessories, the Indian bred, a symbolic accessory of Hippie, was used with various materials. The hippie fashion in 1990's appeared not s a total fashion, but as an ancillary associate has the role of accentuation like hair-style, make-up, acessaries, etc.

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메주에서 분리한 검은 Aspergillus 균주의 동정 (Identification of Black Aspergillus Strains Isolated from Meju)

  • 홍승범;김대호;김선화;방나래;권순우
    • 한국균학회지
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    • 제41권2호
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    • pp.132-135
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    • 2013
  • 검은 Aspergillus 속균은 동양의 발효산업에서 매우 중요한 곰팡이로서 식품산업에 관련된 검은 Aspergillus를 흑국균이라고 한다. 흑국균은 A. luchuensis, A. niger, A. tubingensis로 구성이 되어 있는데 이 중에서 A. luchuensis는 동양의 발효산업에서 매우 중요한 곰팡이로 알려져 있다. 우리나라 전통 된장과 간장의 주요 원료가 되는 메주에서도 검은 Aspergillus가 흔히 발견되었다. 33균주의 검은 Aspergillus가 2008년부터 2011년 사이에 전국적으로 수집한 98개의 완성메주로부터 분리되었고 추가로, 발효과정에 있는 메주로부터 검은 Aspergillus 21균주를 분리하였다. ${\beta}$-tubulin과 calmodulin 유전자 염기서열 분석을 통하여 이들을 동정한 결과 이들은 A. niger 21균주, A. luchuensis 14균주, A. tubingensis 10균주, A. welwitschiae 9균주로 동정되었다. 이 중 A. luchuensis는 생장이 활발하고 단백질, 지방, 탄수화물 분해력이 높은 것으로 조사되어 이들의 산업적 응용에 대한 추가의 연구가 필요한 것으로 사료된다.

농업미생물은행(KACC)의 곰팡이 보존 (Maintenance of Filamentous Fungi in Korean Agricultural Culture Collection (KACC))

  • 신명숙;홍승범
    • 한국균학회지
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    • 제42권2호
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    • pp.97-103
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    • 2014
  • 농업미생물은행(KACC)에는 7,039균주의 곰팡이가 장기 보존되어 있다. 이 중에서 다수의 포자를 형성하는 4,065균주는 동결건조법으로 보존되어 있는데 사멸로 인한 균주의 소실을 막기 위하여 대부분은 액체질소보존법 그리고 모든 균주가 저온냉동고보존법으로 함께 보존되어 있다. 4,065균주의 곰팡이에는 주로 공기 중에 흔하며 산업적으로 이용성이 많은 Aspergillus, Penicillium, Lichtheimia, Mucor, Rhizopus 속 등이 포함된다. 포자를 형성하지 않거나 소수형성하거나 또한 보존이 어려운 너무 큰 포자를 형성하는 나머지 곰팡이는 동결건조보존법으로 보존이 불가하기 때문에 액체질소보존법, 저온냉동고보존법 그리고 광유보존법으로 균주를 보존한다. 여기에는 Phytophthora, Pythium, Cercospora, Septoria, Rhizoctonia 속 등의 식물병원균이 포함된다. 이 외에도 KACC에서 이용하는 다양한 곰팡이 보존법을 소개하고 이들의 상세보존 과정을 기술한다.

일균일명 체계에 의한 국내 보고 Aspergillus, Penicillium, Talaromyces 속의 종 목록 정리 (Species List of Aspergillus, Penicillium and Talaromyces in Korea, Based on 'One Fungus One Name' System)

  • 김현정;김정선;천규호;김대호;석순자;홍승범
    • 한국균학회지
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    • 제44권4호
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    • pp.207-219
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    • 2016
  • 인간의 삶에 큰 영향을 끼치는 Aspergillus, Penicillium 및 그들의 완전세대 속에 속하는 곰팡이의 국내 보고 종 목록을 작성하였다. 기존의 국가 생물종 목록집에는 Aspergillus 14종, Eurotium 4종, Neosartorya 8종, Penicillium 47종, Talaromyces 5종이 보고되었다. 본 논문에서는 국제조류 균류 식물명명규약(ICN)에 따른 일균일명 체계에 따라 Eurotium과 Neosartorya은 Aspergillus 속으로 합치고 Penicillium과 Talaromyces도 새로운 속 개념에 따라 정리하였다. 또한 기존의 국가 생물종 목록에 빠져 있던 77종을 추가하여 Aspergillus, Penicillium 및 그들의 완전세대에 속하는 곰팡이의 국내 보고 종 목록을 Aspergillus 55종, Penicillium 82종, Talaromyces 18종으로 정리하였다.

Analysis of the MAT1-1 and MAT1-2 Gene Ratio in Black Koji Molds Isolated from Meju

  • Mageswari, Anbazhagan;Kim, Jeong-seon;Cheon, Kyu-Ho;Kwon, Soon-Wo;Yamada, Osamu;Hong, Seung-Beom
    • Mycobiology
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    • 제44권4호
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    • pp.269-276
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    • 2016
  • Aspergillus luchuensis is known as an industrially important fungal species used for making fermented foods such as awamori and shochu in Japan, makgeolli and Meju in Korea, and Pu-erh tea in China. Nonetheless, this species has not yet been widely studied regarding mating-type genes. In this study, we examined the MAT1-1 and MAT1-2 gene ratio in black koji molds (A. luchuensis, Aspergillus niger, and Aspergillus tubingensis) and in Aspergillus welwitschiae isolated from Meju, a fermented soybean starting material for traditional soy sauce and soybean paste in Korea. The number of strains with the MAT1-1 locus was 2 of 23 (A. luchuensis), 6 of 13 (A. tubingensis), 21 of 28 (A. niger), and 5 of 10 (A. welwitschiae). Fungal species A. tubingensis and A. welwitschiae showed a 1 : 1 ratio of MAT1-1 and MAT1-2 mating-type loci. In contrast, A. luchuensis revealed predominance of MAT1-2 (91.3%) and A. niger of MAT1-1 (75%). We isolated and identified 2 A. luchuensis MAT1-1 strains from Meju, although all strains for making shochu in Japan are of the MAT1-2 type. These strains may be a good resource for breeding of A. luchuensis to be used in the Asian fermented-food industry.

Iron Increases Susceptibilities of Pseudomonas aeruginosa to Ofloxacin by Increasing the Permeability

  • Kim, Sookyoung;Kim, Jinsook;Hyeran Nam;Yusun Jung;Lee, Yeohee
    • Journal of Microbiology
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    • 제38권4호
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    • pp.265-269
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    • 2000
  • Iron increased the susceptibilities of clinical isolates Pseudomonas aeruginosa to quinolones. In the presence of iron, increased susceptibilities to ofloxacin were observed in twenty-six out of thirty isolates and with no change in four isolates. In the case of norfloxacin, iran increased susceptibilities of twelve isolates but did not render any change in eighteen isolates. In the case of ciprofloxacin, iron decreased the MICs (Minimal Inhibitory Concentration) of twenty isolates, increased the MIC of one isolate, and did net change the MICs of nine isolates. To find out how iron increased susceptibility to ofloxacin, bacterial cells were grown in Muller Hinton (MH) media and succinate minimal media (SMM) to induce iran acquisition systems and the intracellular ofloxacin concentrations were assayed in the presence of iron. The addition of iron to the media decreased the MICs of cells whether they were grown in MH or SMM. Siderophores, carbonyl cyanide m-chlorophenylhydrazone (an inhibiter of proton motive force), and ouabain (an inhibitor of ATPase) did not decrease the effect of iron. Results suggested that the increase in the intracellular ofloxacin concentration by iron is accomplished not by decreasing the efflux but by increasing the of ofloxacin permeability.

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Observations on Some of the Mycelial Growth and Pigmentation Characteristics of Cordyceps militaris Isolates

  • Shrestha, Bhushan;Lee, Won-Ho;Han, Sang-Kuk;Sung, Jae-Mo
    • Mycobiology
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    • 제34권2호
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    • pp.83-91
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    • 2006
  • Characteristic growth patterns of Cordyceps militaris isolates on various media, under varying light conditions and at varying incubation periods were examined. Light was found to be the most critical single factor in determining the density, texture, and pigmentation of the mycelial culture of the fungus. However, under the light condition, the degree of pigmentation and mycelial density were found to be affected by the incubation period and type of medium. Irrespective of the variations in medium type or incubation period, there was no pigmentation of the mycelium under dark condition. Radial growth of the mycelium was faster under dark incubation rather than under light incubation. Abundant mycelial density and darkest pigmentation of C. militaris isolates were produced in nutritionally rich media like SDAY, SMAY and CZYA, suggesting that these media may fulfill all the requirements for vegetative growth of the fungus. Growth characteristics of C. militaris isolates could be easily observed by the simple agar culture method, which would be useful to characterize the phenotypic characteristics of large number of pure cultures of the fungus under given conditions of growth factors such as medium, light and temperature.