• Title/Summary/Keyword: Culture Temperatures

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Novel Starter Culture for Kimchi, Using Bacteriocin-producing Enterococcus faecium Strain (Enterococcus faecium bacteriocin 생산균주를 starter로 이용한 김치의 제조)

  • 하덕모;차동수
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.550-556
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    • 1994
  • For an extension of the palatable stage in Kimchi which was limited by further lowering pH as the fermentation proceeds, the starter culture of bacteriocin-producing Enterococcus faecium DU 0267 obtained from Kimchi was added at the preparation time, and pH, bacteriocin activity, growth of lactic acid bacterial group and gas production in Kimchi were examined during the fermentation at 10, 20 and 30$\circ$C . The pH of Kimchi fell rapidly to 4.0~4.2 in the early fermentation stage, and then, has gone down very slowly throughout further fermentation. The lactic acid bacte- ria, particularly lactobacilli and leuconostoc, were remarkably slower in its growth than those in the control. Although the patterns of these change during fermentation at different temperatures were similar, these effects by the addition of starter were enhanced at 10 and 20$\circ$C. The bacteriocin activity was increased rapidly during log phase of the bacteriocin producer strain in the early fermentation stage of Kimchi and reached their maximum after fermentation at 10$\circC, for 8 days and at 20 or 30$\circ$C for 2 days. Thereafter, the activity disappeared quickly. The gas production by fermentation was also suppressed considerably, and their volume produced after fermentation at 20$\circ$C for 14 days corresponded to 60% of those of the control.

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Quality Characteristics of Dumpling Shell Containing Boehmeria nivea Powder (모시잎 분말 첨가에 따른 만두피의 품질 특성)

  • Park, In-Duck
    • Journal of the Korean Society of Food Culture
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    • v.33 no.3
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    • pp.268-275
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    • 2018
  • This study examined the effects of Boehmerianivea powder on the functional and sensory characteristics of dumpling shell. Various dumpling shell samples were prepared with wheat flour containing different amounts of Boehmerianivea powder. The instrumental characteristics were examined and sensory evaluations were performed. According to the amylograph data, the composite Boehmerianivea powder-wheat flour samples had increased gelatinization temperatures with increasing Boehmerianivea powder content; whereas the initial viscosity at $95^{\circ}C$, viscosity at $95^{\circ}C$ after 15 minutes, and the maximum viscosity were reduced. The L, a and b Hunter's color values decreased with increasing Boehmerianivea powder content. In addition to the cooked weight, the cooked volume and turbidity of dumpling shell increased with increasing level of Boehmerianivea powder. In terms of the textural characteristics, the addition of Boehmerianivea powder increased the chewiness, smoothness and adhesiveness. The DPPH free radical scavenging activity increased significantly with increasing content of Boehmerianivea powder (p<0.05). Finally, the results of the sensory properties indicated that 5% Boehmerianivea powder had the highest scores. These results suggest that Boehmerianivea powder can be applied to dumpling shells to achieve high quality and functionality.

Mechanical and Electrical Properties of Aluminium Alloy by Cryorolling Process

  • Wangkasem, P.;Rojananan, S.
    • International Journal of Advanced Culture Technology
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    • v.3 no.1
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    • pp.46-51
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    • 2015
  • The mechanical and electrical properties of aluminium alloy 6061 are reported in this present work. Aluminium alloys were homogenized at $550^{\circ}C$, for 5 hours and cooled in the furnace. The different thickness reductions of 60-90% on homogenized aluminium alloy plates were achieved by cryo-rolling. Later, the as rolled samples were aged by solution treatment at the temperatures of $520^{\circ}C$ for 1 hour, water quenched; subsequently aged at $160^{\circ}C$ for 8-24 hours and partial aged (not solution treatment) at $160^{\circ}C$ for 8- 24 hours. Mechanical and electrical properties of samples were investigated. The experimental result showed that the microhardness of cryo-rolled samples were increase with increasing the percentage of the thickness reduction. Moreover, the microhardness of cryo-rolled, aged by solution treatment samples were higher than those of the cryo-rolled and cryo-rolled, partial aged samples. The cryo-rolled alloys subjected to full aged at $160^{\circ}C$ for 24 hours exhibited the hardness of 125 HV and electrical conductivity values was 45.76 %IACS and the cryo-rolled alloys subjected to partial aged at $160^{\circ}C$ for 20 hours exhibited the hardness of 67 HV and electrical conductivity values was 49.67 %IACS.

Recovery of Sodium Sulfate from Farm Drainage Salt and Using It in Direct Dyeing of Cotton - Analysis of Color Difference -

  • Jiyoon Jung
    • The International Journal of Costume Culture
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    • v.4 no.1
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    • pp.18-24
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    • 2001
  • Agricultural drainage salt generated during irrigation of crops in San Joaquin Valley, California, exceeds 600,000 tons annually and cumulates in the field in a rapid rate. As a result, the waste is taking out more farmlands for salt storage and disposal, imposing serious concerns to environment and local agricultural industry. In searching for a potential solution to reduce or eliminate the waste, this research explored feasibility of producing a value -added product, sodium sulfate, from the waste and utilizing the product in textile dyeing. The results indicated that sodium sulfate could be produced from the salt and could be purified by a recrystalization method in a temperature range within the highest and lowest daily temperatures in summer in the alley. The recovered sodium sulfate samples, with purities ranging from 67% to 99.91, were compared with commercially available sodium sulfate in direct dyeing of cotton fabrics. The salt samples recovered from Mendata, California (〉98.8% sodium sulfate) cause little color difference in the dyeing with selected direct dyes, and the purified salt (Ⅲ) (99.91% sodium sulfate) is more applicable for direct dyeing of cotton fabrics if it has no other toxic effects. The recovered sodium sulfate from certain areas in the valley could not be employed in direct dyeing due to the high level of impurities in it.

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Inhibition of Aflatoxin Production of Aspergillus flavus by Lactobacillus casei

  • Chang, In-Jeong;Kim, Jeong-Dong
    • Mycobiology
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    • v.35 no.2
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    • pp.76-81
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    • 2007
  • Lactobacillus casei KC-324 was tested for its ability to inhibit aflatoxin production and mycelial growth of Aspergillus flavus ATCC 15517 in liquid culture. flatoxin $B_{1}$ biosynthesis and mycelial growth were inhibited in both simultaneous culture and individual antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolites. produced in cell-free supernatant fluids of the cultured broth of L. casei KC-324. In cell-free supernatant fluids of all media tested, deMan, Rogosa and Sharpe broth, potato dextrose broth, and Czapek-Dox broth+1% yeast extract showed higher antiaflatoxigenic activity. In these case, fungal growths, however, was not affected as measured by mycelial dry weight. The antiaflatoxigenic metabolites from L. casei KC-324 were produced over wide range of temperatures between $25^{\circ}C$ and $37^{\circ}C$. However, these metabolites were not thermostable since the inhibitory activity of the supernatant was inactivated within 30 minutes at $100^{\circ}C$ and $121^{\circ}C$. The inhibitory activity was not influenced by changing pH of supernatant between 4 and 10. However, the antiaflatoxigenic activity was slightly reduced at pH 10.

Quality Characteristics of Mandupi Added with Curcuma aromatica Powder (강황분말을 첨가한 만두피의 품질특성)

  • Park, Bock-Hee;An, Sung-A;Cho, Hee-Sook
    • Journal of the Korean Society of Food Culture
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    • v.29 no.4
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    • pp.348-354
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    • 2014
  • The purpose of this study was to investigate the effects of Curcuma aromatica powder on the functional and sensory characteristics of Mandupi. Various Mandupi samples were prepared with wheat flour along with addition of various amounts of Curcuma aromatica powder, after which instrumental characteristics and sensory evaluations were investigated. According to the amylograph data, the composite Curcuma aromatica powder-wheat flour samples showed increased gelatinization temperatures with increasing Curcuma aromatica powder content, whereas initial viscosity at $95^{\circ}C$, viscosity at $95^{\circ}C$ after 15 minutes, and maximum viscosity were reduced. In terms of Hunter's color values, L value decreased while a and b values increased with increasing Curcuma aromatica powder content. Besides cooked weight, cooked volume and turbidity of Mandupi increased as the addition level of Curcuma aromatica powder increased. In terms of textural characteristics, addition of Curcuma aromatica powder increased springiness, chewiness, and brittleness, whereas springiness and adhesiveness decreased. Based on sensory evaluations, the overall preference of Mandupi with 3% added Curcuma aromatica powder was higher than that of control.

Effects of Low and Alternated Temperature Treatments on Quality of Oak Mushroom in Sawdust Culture (표고 톱밥 재배에서 저온 및 변온 처리가 표고 품질에 미치는 영향)

  • Park, Kyoung-Sub;Son, Jung-Eek;Yoon, Gap-Hee
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.04b
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    • pp.43-44
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    • 2001
  • Recently the sawdust culture of Lentinus edodes(Berk.) has been gradually extended replacing the log cultivation in Korea. It is indeed able to reduce the use of log and cultivation period in controlled facilities, but is not yet able to produce the high-quality mushroom. The objectives of this study were to examine the effects of low and alternated temperature treatments during the fruiting period on the quality of oak mushroom. At low temperature treatments of 1$^{\circ}C$ and 10$^{\circ}C$, the crack, lightness, hardness, and other characteristics for the high-quality oak mushroom were not improved. However, the crack, brightness, and hardness of cap were increased at alternated temperatures of 5-10$^{\circ}C$ than the other temperature treatments. In conclusion, the alternated temperature treatments were more effective than the low temperature treatment for improving the indices of high-quality oak mushroom such as the crack, brightness and hardness of cap.

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Optimization of Maca (Lepidium meyenii) Extraction for Natural Beverage Development using Enzyme Treatment (효소처리에 의한 천연 마카음료 개발을 위한 최적 추출 조건)

  • Kim, Jeong-Ah;Im, Moo-Hyeog
    • Journal of the Korean Society of Food Culture
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    • v.34 no.3
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    • pp.361-368
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    • 2019
  • The purpose of this study was to establish the best optimized extraction condition for the optimal development of fresh maca beverage using low temperature extraction and enzyme treatment. Low temperatures were applied to prevent heat-related nutritional loss during the extraction process. Best extraction conditions were investigated based on the ratio of maca to water, the ratio of enzymes, extraction temperature and time, and agitation. The optimal enzyme conditions were also examined after the treatment of cellulase:pectinase mixture to maintain the original color and flavor, as well as to increase the extraction yield. When cellulase:pectinase was 1:1, the extraction rate ranged from 77.84 to 79.29%. In addition, the best extraction rate was found when maca was mixed with twice volume of water and incubated at $45^{\circ}C$ ($84.05{\pm}0.32%$) with 90 rpm ($87.13{\pm}0.46%$) agitation for 3 hours ($84.73{\pm}0.29%$). Furthermore, sensory evaluation showed a high score in flavor, sweetness, and overall acceptability after adding 3% jujube concentrate into a fresh maca beverage.

Quality Characteristics of Mandupi with Skate (Raja kenojei) flour (홍어 분말을 첨가하여 제조한 만두피의 품질특성)

  • Cho, Hee-Sook;Kim, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.23 no.2
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    • pp.252-257
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    • 2008
  • The purpose of this study was to investigate the effects of skate (Raja kenojei) flour on the functional and sensory characteristics of Mandupi. Mandupi samples were prepared with wheat flour along with the addition of different amounts of skate flour, followed by functional measurements and sensory evaluations. According to the amylograph data, the composite skate flour-wheat flour samples had increased gelatinization temperatures with increasing skate flour content; whereas initial viscosity at $95^{\circ}C$, viscosity at $95^{\circ}C$ after 15 minutes, and maximum viscosity were reduced. In terms of color values, L and b values decreased, but the a-value increased with increasing skate flour content. Furthermore, the addition of skate flour increased hardness, springiness, chewiness, and adhesiveness in the samples. Overall, based on sensory evaluations, the Mandupi containing 5% skate flour was preferred over the other samples.

Characterization of Endochitosanases-Producing Bacillus cereus P16

  • Jo, Yu-Young;Jo, Kyu-Jong;Jin, Yu-Lan;Jung, Woo-Jin;Kuk, Ju-Hee;Kim, Kil-Yong;Kim, Tae-Hwan;Park, Ro-Dong
    • Journal of Microbiology and Biotechnology
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    • v.13 no.6
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    • pp.960-968
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    • 2003
  • A bacterial isolate showing a strong endochitosanase activity was isolated from soil and then characterized. The isolate was identified and designated as Bacillus cereus P16, based on morphological and biochemical properties, assimilation tests, cellular fatty acids pattern, along with 16S rRNA gene sequence. The optimized medium for producing extracellular chitosanase in a batch culture contained 1% tryptone, 0.5% chitosan, and 1% NaCl (pH 7.0). Powder chitosan and tryptone served the best as carbon and nitrogen sources, respectively, for the chitosanase production. Chitosanase activity was the highest when culture was completed at $37^{\circ}C$ among various temperatures ($20-42^{\circ}C$) tested in a shaking incubator (200 rpm). The levels of chitosanase activity in the culture fluid were 2.0 U/ml and 3.8 U/ml, respectively, when incubated in a flask for 60 h and in a jar fermenter for 24 h. The culture supernatant showed a strong liquefying activity on the soluble chitosan. The viscosity of 1% chitosan solution, that was incubated with the culture supernatant, was rapidly decreased, suggesting the secretion of endochitosanolytic enzymes by P16. The culture fluid revealed six endo-type chitosanase isozymes, two major (38 and 45 kD), and four minor (54, 65, 82, and 96 kD) forms by staining profile. The crude enzymes were very stable, and full activity was maintained for 4 weeks at $4^{\circ}C\;or\;-20^{\circ}C$ in the culture supernatant, suggesting a highly desirable stability rate for making an industrial application of the crude enzymes. The supernatant also cleaved the insoluble chitosan powder, but the hydrolysis rate was much lower. The enzymic degradation products of chitosan contained $(GlcN)_n$ (n=2-8). The concentration of chitosan in the reaction mixture of the crude enzyme affected the chitooligosaccharides composition of the hydrolysis products. When the higher concentration of chitosan was used, the higher degree of polymerized chitooligosaccharides were produced. By comparison with other commercial chitosanase preparations, P16 was indeed found to be a valuable enzyme source for industrial production of chitooligosaccharides from chitosan.