• 한국산림과학회지
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• 제103권2호
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• pp.203-210
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• 2014

본 연구는 지구 온난화와 관련한 대응 대책 마련을 위해서 대기 온도 상승에 따른 주요 침엽수의 종자 발아 및 발아 후 유묘의 생존율 변화를 알아보고자 수행하였다. 소나무와 전나무를 대상으로 적외선등을 이용하여 대조구 보다 온난화 처리구의 대기 온도를 $3^{\circ}C$ 높게 유지하는 실외 실험적 온난화 처리를 실시하였다. 온난화 처리에 따른 대기 온도 상승으로 두 수종 모두 발아율이 높아지는 경향을 보였지만, 전나무에서만 유의적 차이가 나타났다. 소나무와 전나무 두 수종 모두 온난화 처리구가 대조구보다 평균발아일수는 유의적으로 감소하였으며, 온난화 처리구에서 높은 발아속도와 발아세를 보였다. 온도 상승과 토양 수분 감소에 따라 전나무 유묘의 고사율은 증가하였지만, 소나무는 온난화 처리에 따른 차이를 보이지 않았다. 향후, 지구 온난화에 따른 대기와 토양 온도 상승 및 토양 수분감소로 양묘과정에서 종자 발아 및 유묘 생존율의 변화가 예상되기 때문에 지구 온난화에 대응할 수 있는 양묘기술 개선이 필요할 것으로 판단된다.

• The Korean Journal of Physiology
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• 제3권1호
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• pp.19-28
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• 1969

Skin temperatures on 10 sites and rectal temperature at every 10 minutes, oxygen consumption at every 20 minutes were measured on 18 male subjects (ages between 14 and 47 years) after exposure to cold air at $15^{\circ}C$ for two hours in a climatic room. Total body fat measured by means of a skinfold method and ratio of body surface area (S) to body volume (V), S/V, were utilized as basis of observations. Surface area was calculated after DuBois equation and body volume was calculated by our original formula. In influencing on the heat loss from the body core to the cold environment, % fat showed inverse relations, whereas, S/V ratio showed direct relations. Thus these two factors acted antagonistically on the body heat loss. Local skin temperatures showed negative correlations with skinfold thickness on the same site, nemaly, on chest, r=-.567; on back, r=-.507; and on upper arm, r=-.353. The other 7 skin sites showed low correlations with % fat. Minimum mean weighted skin temperature (MWST) showed a negative correlation (r=-.443) with % fat, and showed no correlation with S/V ratio. Oxygen consumption in the cold air at $15^{\circ}C$ increased from the first measurement at 20 minutes after exposure and maintained the same increasing trend up to 120 minutes. ${\Delta}T_R$ was greater in tile lean subjects who showed a greater % change in oxygen consumption. The antagonistic actions of % fat and S/V ratio on the heat loss were manifested by observations as follows: minimum rectal temperature was higher In fat subjects (r=.600) and lower in subjects with a greater S/V ratio (=-.582), ${\Delta}T_R$ was smaller in fat subjects (r=-.738) and greater in subjects with a greater S/V ratio (r=.618). Temperature difference between body core and skin surface (minimum rectal temperature minus minimum MWST) showed a positive correlation with % fat (r=.600) and a negative correlation with S/V ratio (r=-.881). Decrease in the mean body temperature and heat debt, respectively, showed negative correlations with % fat and positive correlations with S/V ratio.

• 한국식생활문화학회지
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• 제9권3호
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• pp.303-310
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• 1994

This study was conducted to assess the acceptability of the desirable serving temperatures of soups by evaluating the sensory characteristics of Radish Clear Beef Soup, Meat Pot Stew, and Chinese Cabbage Beanpaste Soup used in the Foodservice Establishment. 1. Taste revealed a better acceptability under a high temperature than that under a low temperature in three soups. 2. The aroma of Meat Pot Stew, Chinese Cabbage Beanpaste Soup showed a significant difference for each temperature, and especially, Chinese Cabbage Beanpaste Soup showed a better sabory aroma at $80^{\circ}C{\sim}85^{\circ}C$. 3. Meat Pot Stew showed little significant differences in color and in its change among three soups. Chinese Cabbage Beanpaste Soup did not make any change of color at both $35^{\circ}C{\sim}40^{\circ}C\;and\;50^{\circ}C{\sim}55^{\circ}C$. 4. In a Meat Pot Stew case, appearance conspicuously changed at $35^{\circ}C{\sim}40^{\circ}C$, which resulted in losing appetite. Three soups showed a good response above at $65^{\circ}C$ and a very poor response at below $55^{\circ}C$. 5. Radish Clear Beef Soup, Meat Pot Stew, Chinese Cabbage Beanpaste Soup showed a tender texture at above $80^{\circ}C,\;65^{\circ}C\;and\;50^{\circ}C$. 6. Chewiness showed a good acceptability from $50^{\circ}C\;to\;80^{\circ}C$ in Radish Clear Beef Soup, Chinese Cabbage Beanpaste Soup cases, but only in the case of Meat Pot Stew showed good response at $80^{\circ}C{\sim}85^{\circ}C$. 7. In cases of Radish Clear Beef soup, Meat Pot Stew, bland and stinking aroma, and aftertaste were increased remarkably in proportion as temperature was lowered. In case of Meat Pot Stew, especially, it turned out to be almost impossible to eat at $35^{\circ}C{\sim}40^{\circ}C$. 8. In cases of Radish Clear Beef Soup, Chinese Cabbage Heanpaste Soup, overall acceptability at $65^{\circ}C$ was comparatively good but aroma and taste at below $65^{\circ}C$ was so bad that there was the eminent tendency to avoid tasting.

• 한국식생활문화학회지
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• 제24권2호
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• pp.212-218
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• 2009

This study was carried out to determine the quality characteristics of Jeju mandarin orange jellies containing sugar (control) and sugar derivative sweeteners (erythritol, isomaltooligosaccharide, sorbitol, and xylitol) to be consumed by the elderly. The gelling agents were carrageenan and gellan gum at the concentration of 0.27%, respectively. The average age of the subjects for the acceptance test was 78. The lightness (L), redness (a), and yellowness (b) values of the carrageenan gel were higher than those of the gellan gum gel using the same sugars. The L, a, and b values of the carrageenan gel made with sugar derivative sweeteners were higher than those of the respective gel made with sugar. Whereas there were almost no differences between the color values of the gellan gum gels made with sugar and sugar derivative sweeteners, respectively. The gelling and melting temperatures of the carrageenan gel with erythritol were highest and those of the respective gel with xylitol were lowest among the sugar derivative sweeteners. The gelling and melting temperatures of the gellan gum gels with erythritol and isomaltooligosaccharide, respectively, were lower, and those of the respective gel made with xylitol were higher compared to that with sugar. There was no break down in the gellan gum gel. The break down rate of the carrageenan gel made with isomaltooligosaccharide was highest and that of the respective gel with xylitol was lowest among the sugar derivative sweeteners. The hardness, adhesiveness, springiness, and chewiness of the gellan gum gel were greater compared to the carrageenan gel. The adhesiveness levels of the carrageenan gels made with sugar derivative sweeteners were lower, and their springiness was higher than the respective gel with sugar. All the TPA characteristics of the gellan gum gels made with erythritol and xylitol, respectively, were higher than the respective gel with sugar. The rupture properties of the gels were the same as their TPA characteristics. The sensory acceptance of the carrageenan and gellan gum gels made with sugar were highest among all the sweeteners. There were no significant differences in the sensory acceptance scores of the carrageenan gels made with xylitol, erythritol, and sugar, respectively, and there were also no significant differences in the sensory acceptance scores of the gellan gum gels made with xylitol, sorbitol, and sugar, respectively. Xylitol was preferred in both the carrageenan and gellan gum gels. Thus, xylitol appears to be an appropriate alternative sweetener to sugar in Jeju mandarin orange jelly for consumption by the elderly, with regard to the acceptability and stability of the gel.

• 한국버섯학회지
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• 제18권4호
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• pp.317-322
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• 2020

느타리 '흑타리' 품종의 배양 중 고온스트레스에 의해 발생되는 미발이 현상을 구명하기 위하여 배양온도에 따른 생육차이를 조사하였다. PDA 배지에서 '흑타리'의 적정생육온도는 23~26℃였고, 균사생장속도는 '춘추2호'에 비하여 빠른편이었다. 병내 배지온도는 초기에 상승하여 배양 중반에 최고점에 도달한 후 온도가 하강하였다. 배양 온도가 높을수록 배양기간은 짧아졌다. 배양온도 20℃ 처리구에서 배양기간은 25일 정도 소요되었으며, 미발이율은 1.8%, 수량은 139.4 g/병을 나타내었다. 배양온도 24℃ 처리구에서 배양기간은 20일 정도 소요되었으며, 미발이율은 4.2%, 병당수량은 132.1 g/병을 나타내었다. 배양온도 16℃와 28℃처리구에서는 미발이율이 증가되었고 수량이 감소하였다. 이 결과를 바탕으로 농가에서 배양온도와 미발이율의 관계를 조사하였다. 배양실 온도를 18℃로 설정하고 배지품온을 28℃ 미만으로 관리하는 농가는 미발이율이 0.3~0.8%를 나타내었다. 배양실내의 온도가 20℃ 이상이며 환기가 잘 이루어지지 않은 농가에서는 미발이율이 3.5% 정도 발생되었다. 배양실 온도가 19℃이며 배지 최고 품온이 31.3℃까지 상승하는 농가는 미발이율이 8.2%로 높게 나타났다. 배양중 병내부 온도가 28℃이상 상승하고 배양실내의 환기가 잘 이루어지지 않을 경우 미발이율이 증가하고 수량이 감소되는 경향을 보였다. 결과적으로, 배양실은 배지품온이 28℃ 이상 상승하지 않도록 배양실 내부의 공기를 지속적으로 순환시키고, 배양공간에 맞는 최적의 배양량을 넣어 관리하여야 한다.

• Ocean and Polar Research
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• 제32권1호
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• pp.63-71
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• 2010

An experiment was conducted to investigate the effects of temperature and stocking density on daily patterns and rates of total ammonia nitrogen (TAN) excretion in juvenile red seabream Pagrus major (mean body weight: 29.0 g) under fasting and feeding conditions. Fish were acclimated over 7 days under four different temperatures (10, 15, 20, and $25^{\circ}C$) and at two different densities (5.5 and $11.0\;kg\;m^{-3}$). Each treatment had three replicates and a total of 216 fish were used. After 72 hours starvation, endogenous TAN excretion was measured for each temperature and density. To investigate exogenous TAN excretion, fish were handfed a commercial diet containing 51.6% crude protein twice a day for 7 days, at 08:00 and 16:00. Water was sampled from both inlets and outlets of chambers every 2 hours over a 24 hour period. Both endogenous and exogenous TAN excretion increased with increases in temperature and density (P<0.05). Mean daily endogenous TAN excretion rates at 10, 15, 20, and $25^{\circ}C$ were 88.8, 101.1, 125.0, and $143.3\;mg\;TAN\;kg^{-1}\;d^{-1}$ at low density, and 105.2, 119.2, 141.5, and $168.8\;mg\;TAN\;kg^{-1}\;d^{-1}$ at high density, respectively. Mean daily exogenous TAN excretion rates at 10, 15, 20, and $25^{\circ}C$ were 343.5, 403.7, 535.7, and $601.7\;mg\;TAN\;kg^{-1}\;d^{-1}$ at low density, and 391.9, 479.7, 611.9, and $683.4\;mg\;TAN\;kg^{-1}\;d^{-1}$ at high density, respectively. The exogenous TAN excretion rate peaked 10~12 hours after the first feeding under all temperatures and densities. The TAN loss for ingested nitrogen increased with increases in temperature and density (P<0.05), ranging from 27.9 to 50.1% at low density and 31.7 to 56.9% at high density. This study provides empirical data for estimating ammonia excretion and managing the culture of red seabream under the given temperatures and densities.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.127-133
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• 2006

Shewanella oneidensis MR-1 has the ability to inhale certain metals and chemical compounds and exhale these materials in an altered state; as a result, this microorganism has been widely applied in bioremediation protocols. However, the relevant characteristics of cell growth and biosynthesis of PuFAs have yet to be thoroughly investigated. Therefore, in this study, we have attempted to characterize the growth and fatty acid profiles of S. oneidensis MR-1 under a variety of temperature conditions. The fastest growth of S. oneidensis MR-1 was observed at $30^{\circ}C$, with a specific growth rate and doubling time of $0.6885h^{-1}\;and\;1.007 h$. The maximum cell mass of this microorganism was elicited at a temperature of $4^{\circ}C$. The eicosapentaenoic acid (EPA) synthesis of S. oneidensis MR-1 was evaluated under these different culture temperatures. S. oneidensis MR-1 was found not to synthesize EPA at temperatures in excess of $30^{\circ}C$, but was shown to synthesize EPA at temperatures below $30^{\circ}C$. The EPA content was found to increase with decreases in temperature. We then evaluated the EPA biosynthetic pathway, using a phylogenetic tree predicted on 16s rRNA sequences, and the homology of ORFs between S. oneidensis MR-1 and Shewanella putrefaciens SCRC-2738, which is known to harbor a polyketide synthase (PKS)-like module. The phylogenetic tree revealed that MR-1 was very closely related to both Moritella sp., which is known to synthesize DHA via a PKS-like pathway, and S. putrefaciens, which has been reported to synthesize EPA via an identical pathway. The homology between the PKS-like module of S. putrefaciens SCRC-2738 and the entire genome of S. oneidensis MR-1 was also analyzed, in order to mine the genes associated with the PKS-like pathway in S. oneidensis MR-1. A putative PKS-like module for EPA biosynthesis was verified by this analysis, and was also corroborated by the experimental finding that S. oneidensis MR-1 was able to synthesize EPA without the expression of $dihomo-{\gamma}-linoleic$ acid (DGLA) and arachidonic acid (AA) formed during EPA synthesis via the FAS pathway.

• 한국식품위생안전성학회지
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• 제12권1호
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• pp.9-14
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• 1997

Vibrio mimicus is a closely related species with V. cholerae, and has been reported to be associated with gastrointestinal infections. Although extraintestinal infections of these vibrios have also been reported in Japan and Southeast Asia. But little research papers on V. mimicus was reported in Korea. Therefore, we tried to isolate V. mimicus from the environmental sea water from April to July in Pusan, Korea. Among the isolated strains, we selected the strongest hemolytic strain and then named V. mimicus SM-9. In this paper, we checked the antibiotic susceptibility and psychrotrophic characteristics of the isolated strain. Hemolytic activity of the hemolysin produced by the isolated strain was also measured. V. mimicus was not detected from the sea water samples in April and May, but its detection rate was relatively high in June and July in Pusan, Korea. The bacteriological characteristics of V. mimicus SM-9 were Gram-negative rods, motile, oxidase positive, Voges-Proskauer negative and sucrose negative. In 23 kinds of antibiotics susceptibility test, V. mimicus SM-9 showed susceptibility to the most of antibiotics submitted while it was resistive against lincomycin, oxacillin, rifampin and vancomycin. Hemolytic activity of the hemolysin produced by V. mimicus SM-9 was highest in stationary phase of the growth curve in BHI broth at 37$^{\circ}C$ and its activity was reached 18 HU per $m\ell$ of culture supernatant. For checking the psychrotrophic property of V. mimicus SM-9, the decreasing rate of the strain in phosphate buffer solution and yellowtail flesh homogenate was examined during the storage at 4, 0, -4 and -2$0^{\circ}C$. The decreasing rates of the selected strain stored in phosphate buffer solution were greater than those in fish homogenate. Decreasing rates of V. mimicus SM-9 stored in phosphate buffer solution were not significantly different by the storage temperatures. The viable cell counts of the strain were decreased as 5 log cycles after 120 hours at all the tested temperatures. While decreasing numbers of the strain in fish homogenates were 2*4 log cycles after 120 hours. The decreasing pattern of the strain numbers were very slow after 200 hours at all the stored temperatures.

• 한국포장학회지
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• 제29권1호
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• pp.35-41
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• 2023

수경재배한 미니 파프리카 '뉴라온레드'을 골판지 골판지 박스로 포장하여 저장 시, 온도조건 별(5℃, 10℃, 15℃와 20℃) 저장에 따른 특성 변화를 조사하였다. 일반적으로 파프리카는 10℃에서 저장이나 유통이 적절한 것으로 알려져 있으나, 국내 미니 파프리카는 상온에서 온도관리를 하지 않고 수확후 관리가 이루어지고 있었다. 미니 파프리카 '뉴라온레드'의 외관변화는 저장온도 5℃, 10℃와 15℃에서 비슷한 경향으로 나타나는데, 저장온도 10℃에서 상품성 유지기간이 가장 길었다. 저장온도 20℃에서는 다른 저장온도에서보다 급격한 외관변화로 인해 상품성 감소로 선도를 유지하는 기간이 짧아졌다. 생체중량 변화는 저장온도20℃가 저장온도 5℃~15℃와 차이를 보였으며, 저장기간이 지속될수록 격차가 더 커졌다. 생체중량 변화에서 저장온도 5℃~15℃에서는 유사한 경향을 보였다. 미니 파프리카 저장 중 경도 감소 정도는 온도에 따라 영향을 받아, 20℃에서 가장 커서 경도가 낮아졌으며, 다른 저장온도에서 저장 종료 시 저장온도 10℃에서 다소 높은 경도 유지의 경향을 보였다. Hue angle 변화는 저장초기부터 차이를 인지할 수 있었는데, 저장온도 15℃와 20℃에서 변화폭이 저장온도 5℃와 10℃에서보다 커서, 낮은 수준을 보였다. 그러나 미니 파프리카의 저장온도에 따른 가용성 고형분 함량(SSC) 변화는 저장온도에 따른 영향이 불분명한 것으로 나타났다. 수경재배한 미니 파프리카의 저장온도에 따른 수확후 특성은 높은 저장온도에서 모든 변화량이 크고 선도 감소가 커서, 현행의 상온 유통을 개선한 온도관리가 필요한 것으로 보인다. 다른 저장온도인 15℃와 20℃에서보다 5℃와 10℃에서 품질변화폭이 적어, '뉴라온레드'의 수확후 상품성 유지에 효과적인 관리온도로 판단된다. 본 연구를 통하여 미니 파프리카 '뉴라온레드'의 수확후 관리에 필요한 적정온도를 제시하였으며, 향후 다른 품종으로까지 연구를 확대하여 미니 파프리카의 관리를 일반화된 결과를 제시하여 보편적 관리기술로 정착화가 필요한 것으로 보인다.

• 한국어병학회지
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• 제6권2호
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• pp.205-218
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• 1993

On the development of hirame(Paratichtys olivaceus) culture, outbreak of scuticociliata infection was reported to cause severe damage in Japan. To establish effective measures for isolation and cultivation of this ciliate, we tried to culture this pathogenic ciliate using medium for bacteria and fish cell lines in vitro. Scuticociliata from the brain tissues of infected fish was aseptically inoculated to CHSE-214 cells cultured in MEM-10 without antibiotic. Scuticociliata grew well and the number of ciliate reached $10^6\;cells/ml$ at temperatures of $15^{\circ}C$ to $20^{\circ}C$ for 10d. The number of ciliate cultured in the cell lines is 10 times higher than the numbers cultured in the liquid medium alone. This ciliata could be cloned by dilution method. Scuticociliata isolated could grow well on 42 different cell lines that were established from marine fish, warm freshwater fish, and salmonids. This ciliate could be preserved in liquid nitrogen for more than 6 months. Subsequently, we observed the optimal temperature and salinity for growth, and tested the sensitivities of this organism to formaldehyde, flagyl(Metronidazole), Ekuteshin(Combination compound of sulfamonometoxin and ormethoprim), and ozonixation. Optimal temperature for growth was $25^{\circ}C$ and salinity was 1.0 to 1.5%. Washed scuticociliata was killed by formaldehyde at the concentration of 50ppm for 10min, but was not completely killed even at a high concentration of 400ppm for 20min in MEM-5. Flagyl and Ekuteshin can inhibit the growth of scuticociliata at the concentration of 1,000 and $100{\mu}g/ml$ in MEM-10, respectively. More than 99% of this scuticociliata could be killed by ozonization at a dose equivalent to $1.0mg/\ell$ oxidant for 30sec in sea water. Isolated scuticociliata showed the pathogenicity to the cultured hirame by artificial infection(I. P. injection, $10^5\;cells$/fish). The number of scuticociliata in the water could be counted by most probable number(MPN) method using tissue culture, and the minimum detectable number was $1.8\;cells/\ell$. The number in the reservoir tank for water supply to the culture tank was 110 cells/l. After cleaning by elimination of the sediments from of the reservoir tank and disinfected with formaldehyde, number of scuticociliata decreased and was counted less than $1.8\;cells/\ell$ and infection rate of cultured hirame was decreased.