• Title/Summary/Keyword: Culture Diffusion

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Inhibitory Effects of Bile Acids on the Cholesterol Biosynthesis in Cultured Hepatocytes (배양 간세포내에서의 콜레스테롤 합성에 대한 담즙산의 저해효과)

  • Kim, Sung-Wan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.5
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    • pp.496-501
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    • 1992
  • The present work tested the inhibitory effects of bile acids on the cholesterol biosynthesis and the activity of HMG-CoA reductase in cultured rat hepatocytes. The uptake of bile acids in hepatocytes were increased in according to the different bile acid concentrations and culture times. The rate of cholesterol synthesis in cells were inversely decreased to the bile acid concentrations and culture times. As expected, insulin injection (4 units/100g body weight) showed an enhancing effect of the cholesterol synthesis and the HMG-CoA reductase activity. The addition of bile acids in medium of insulin-treated hepatocytes also showed the suppressing effect. This effect was directly confirmed in isolated hepatic icrosomes by the test of HMG-CoA reductase activity. In the test of $Na^+$,$K^+$-ATPase activity in the isolated hepatocyte membrane, only the cholic acid did not stimulate the enzyme system. The reason of such difference is not obvious, but this result indicates that the cholic acid could be absorbed by simple diffusion.

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Woman Blogger's Health Image Recognition of Korean Foods (여성 블로거의 한식 건강 이미지에 대한 인식)

  • Chung, Hea-Jung;Cheon, Hee-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.20 no.6
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    • pp.837-844
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    • 2010
  • Dietary life was formed as being influenced by the people's cultural, social and environmental situations. The people's food culture is unique that was adapted to their surroundings. It was developed and industrialized in the ethnic food. Now the ethnic food was contributed to tour industry or culture industry. Then, food life style was change from the meat diet to the vegetable diet in the diffusion of well-being trends. So, we analyzed Koreans' Korean food image and investigated the difference as a demographics. We made questionnaire and did a survey to 220 bloggers using cooking internet sites on 10 August, 2009. We analyzed 206 questionnaires by SPSS package 12.0 except 14 untrustworthy questionnaires. We measured credibility and validity 39 items related Korean food image: Chronbach's ${\alpha}$ was highly 0.855. We checked that KMO examination was 0.775 and $x^2$ of Barttlet was 2482.342. After verymax rotation, we deducted 5 Koran food images-healthy, visuality, spicy, variety, low calorie. We analyzed ANOVA of 5 Korean food images according to demographics. We confirmed the differences by ages, monthly income and job except academic background (p<0.05). Therefore, Korean food images were learned and recognized in everyday life. Especially, we found that visuality and variety were recognized the more stronger high-income earners and teachers than others. So, Korean learned Korean food images in real life and recognized Korean food quite differently by demographics.

Study on Characteristics and User Reactions of Videos Related to COVID-19 Vaccine (코로나19 백신 관련 영상의 특성 및 이용자 반응에 대한 연구)

  • Lee, Mina;Hong, Juhyun
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.3
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    • pp.163-171
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    • 2021
  • This study aimed to investigate the main characteristics of the COVID-19 vaccine-related videos spread on YouTube and differences in user responses in the infodemic situation caused by COVID-19. As a result of content analysis of 579 videos related to the COVID-19 vaccine, it was found that all of the false information was written by individual channels. Institutions, organizations, media companies, and government channels reported spread of false information as well as fact-oriented reporting. The progressive channel had a high percentage of positive sentiment in favor of vaccination, and the conservative channel had a high percentage of negative emotion against vaccination. After the vaccination started, the number of videos on government channels increased, and it was found that the number of videos with positive emotions increased. Results of regression analysis of video characteristics that affect the number of likes indicated that personal expert videos and videos from progressive channels received more likes. Combining the research results, we propose a plan to promote government policies regarding the COVID-19 vaccine using social media.

A novel antimicrobial-containing nanocellulose scaffold for regenerative endodontics

  • Victoria Kichler ;Lucas Soares Teixeira ;Maick Meneguzzo Prado ;Guilherme Colla ;Daniela Peressoni Vieira Schuldt ;Beatriz Serrato Coelho ;Luismar Marques Porto ;Josiane de Almeida
    • Restorative Dentistry and Endodontics
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    • v.46 no.2
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    • pp.20.1-20.11
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    • 2021
  • Objectives: The aim of this study was to evaluate bacterial nanocellulose (BNC) membranes incorporated with antimicrobial agents regarding cytotoxicity in fibroblasts of the periodontal ligament (PDLF), antimicrobial activity, and inhibition of multispecies biofilm formation. Materials and Methods: The tested BNC membranes were BNC + 1% clindamycin (BNC/CLI); BNC + 0.12% chlorhexidine (BNC/CHX); BNC + nitric oxide (BNC/NO); and conventional BNC (BNC; control). After PDLF culture, the BNC membranes were positioned in the wells and maintained for 24 hours. Cell viability was then evaluated using the MTS calorimetric test. Antimicrobial activity against Enterococcus faecalis, Actinomyces naeslundii, and Streptococcus sanguinis (S. sanguinis) was evaluated using the agar diffusion test. To assess the antibiofilm activity, BNC membranes were exposed for 24 hours to the mixed culture. After sonicating the BNC membranes to remove the remaining biofilm and plating the suspension on agar, the number of colony-forming units (CFU)/mL was determined. Data were analyzed by 1-way analysis of variance and the Tukey, Kruskal-Wallis, and Dunn tests (α = 5%). Results: PDLF metabolic activity after contact with BNC/CHX, BNC/CLI, and BNC/NO was 35%, 61% and 97%, respectively, compared to BNC. BNC/NO showed biocompatibility similar to that of BNC (p = 0.78). BNC/CLI showed the largest inhibition halos, and was superior to the other BNC membranes against S. sanguinis (p < 0.05). The experimental BNC membranes inhibited biofilm formation, with about a 3-fold log CFU reduction compared to BNC (p < 0.05). Conclusions: BNC/NO showed excellent biocompatibility and inhibited multispecies biofilm formation, similarly to BNC/CLI and BNC/CHX.

Applying Appropriate Technology Design in North Korea: An Exploration (대북적정기술 디자인의 도입 및 적용에 대한 탐구)

  • Xianglian Han;Sung Woo Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.141-151
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    • 2023
  • Traditional ODA to North Korea (NK) has been unsustainable and politically contentious. This study proposes the design of appropriate technology (AT) as an alternative. We identified NK residents' urgent needs and global AT instances, and classified data by Maslow's hierarchy of needs. As electricity and potable water were identified as primary needs, suitable AT cases were selected. Given NK's extreme isolation, collaborative AT practices with local residents are unfeasible. Therefore we propose a new AT adoption model customized to NK, which emphasizes community-level adoption. We proposed a solar charging station for cooperative farms, a re-design of an AT previously utilized in Africa, and tailored it to fit our proposed model. The study's significance lies in its novel AT adoption model for NK's unique social fabric and the proposition of a specific design case, thus transcending previous relevant studies exploring AT's potential for NK.

A Study on a Perception and Promotion Plan for Employees to Increase the Consumption of Low-Sodium Foods Using Importance Performance Analysis (IPA) - Focused on Ready-to-Eat Foods (Samgak-Kimbap, Riceballs) - (식품 종사자가 인식하는 저나트륨 식품과 소비 확대를 위한 중요도-수행도 조사 연구 - 즉석섭취식품(삼각김밥, 주먹밥)을 중심으로 -)

  • Jimin Lim;Suah Moon;Ga Eun Yeo;Eui Su Kim;Yuri Kim;Ji Eun Oh
    • Journal of the Korean Society of Food Culture
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    • v.38 no.3
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    • pp.129-142
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    • 2023
  • As the demand for ready-to-eat foods continues to grow, concerns about the sodium in processed foods are also growing. In this study, a survey was conducted on the perception of low-sodium products and diffusion plans according to the type of employee (manufacturer, retailer, distributor). Of the 191 responses collected, 189 valid responses (98.9%) were analyzed. The results showed that the employees were aware of the health contribution of a low-sodium diet, labeling for low-sodium, and the promotion of low-sodium food to increase its consumption. Furthermore, retailers recognized the positive contribution of low-sodium products in terms of Environmental, Social, and Governance (ESG) management. The use of sodium substitutes was preferred as the best way to reduce sodium in ready-to-eat foods. With regard to sodium reduction in ready-to-eat foods, we found that the technical factors involved were clustered by the type of business. Specifically, distributors showed a similar performance but had a lower perception of importance than retailers. Manufacturers had a lower perception of both importance and performance. In this study, we collected perceptions from employees who were working at food companies, which differed from previous research. We sought to examine the differences in the perception of sodium reduction and consumption of ready-to-eat foods across various types of employment. Furthermore, we provided specific approaches to reduce perception gaps and enhance understanding among employees.

Empirical Research on the Interaction between Visual Art Creation and Artificial Intelligence Collaboration (시각예술 창작과 인공지능 협업의 상호작용에 관한 실증연구)

  • Hyeonjin Kim;Yeongjo Kim;Donghyeon Yun;Hanjin Lee
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.1
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    • pp.517-524
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    • 2024
  • Generative AI, exemplified by models like ChatGPT, has revolutionized human-machine interactions in the 21st century. As these advancements permeate various sectors, their intersection with the arts is both promising and challenging. Despite the arts' historical resistance to AI replacement, recent developments have sparked active research in AI's role in artistry. This study delves into the potential of AI in visual arts education, highlighting the necessity of swift adaptation amidst the Fourth Industrial Revolution. This research, conducted at a 4-year global higher education institution located in Gyeongbuk, involved 70 participants who took part in a creative convergence module course project. The study aimed to examine the influence of AI collaboration in visual arts, analyzing distinctions across majors, grades, and genders. The results indicate that creative activities with AI positively influence students' creativity and digital media literacy. Based on these findings, there is a need to further develop effective educational strategies and directions that incorporate AI.

A Study on the Spread of YouTube Political Issues and the Attribution of the Issue, Focusing on the Issue of the Constitutional Court's Ruling on the 'Complete deprivation of prosecutorial powers' Act (유튜브 정치 이슈의 확산 양산과 이슈 속성 연구: '검수완박' 법안 헌법재판소 판결 이슈를 중심으로)

  • Insool Cho;Juhyun Hong
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.1
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    • pp.193-203
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    • 2024
  • In a situation where news usage through YouTube is rapidly increasing, this study investigated which attributes of issues news producers prominently report on based on the two-stage agenda setting theory to empirically investigate the influence of various news producers on YouTube. Through the research results, we confirmed that broadcasters have the influence to set the agenda and form public opinion on YouTube, and discovered the possibility of a two-stage agenda setting effect occurring in the YouTube environment. We criticized whether news producers abuse emotional words due to their partisanship when reporting political issues, and discussed that an emotional approach to political issues can have a negative impact on news users' perception of reality.

Comparison of the Biochemical Activities of Commercial Yogurts and Lactobacillus acidophilus-containing Yogurt (시판용 요구르트와 Lactobacillus acidophilus 요구르트의 생화학적 활성의 비교)

  • Ryu, Jae-Ki;Lee, Hyeong-Seon;Koo, Bon-Kyung;Kim, Hyun-Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.2
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    • pp.59-64
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    • 2015
  • Lactic acid-producing bacteria such as Lactobacillus spp. function to ferment carbohydrates and produce ATP. Such Lactobacillus spp. are used for the production of commercial yogurts. Lactobacillus spp. are beneficial to the intestinal tract, and Lactobacillus acidophilus-containing yogurts have received considerable attention because of their preventive effects against early-stage cancer of the large intestine. In this study, lactic acid-producing bacteria were cultured from three different groups: commercial solid yogurt (for eating), commercial liquid yogurt (for drinking), and Lactobacillus acidophilus-containing yogurt. We first determined the optimum culture conditions for Lactobacillus spp. and then analyzed turbidity and pH in order to compare the growth abilities and lactic acid-production capacities among the groups. Finally, high-performance liquid chromatography was used to measure the lactic acid content in the culture supernatants, and the antibacterial activities against Staphylococcus aureus and Escherichia coli were compared among the three groups. The optimum culture conditions for Lactobacillus spp. were MRS medium at $25^{\circ}C$, for 24 h. The highest turbidity was found in L. acidophilus-containing yogurt, followed by liquid yogurt and solid yogurt. Similarly, the highest lactic acid production ability was found in L. acidophilus-containing yogurt, followed by liquid yogurt and solid yogurt. Culture supernatants from the three groups did not show any antibacterial activity towards S. aureus; however, supernatants derived from L. acidophilus-containing yogurt resulted in a 1.8 mm inhibitory zone against E. coli in a paper disk diffusion test. These results revealed the high level of lactic acid-production capacity and antibacterial activity in L. acidophilus-containing yogurt.

The Meaning of the 'Collective Intelligence' in the Transmedia Discourse (트랜스미디어 담론에 대한 집단지성론적 고찰)

  • Kim, Ki-Hong
    • Cartoon and Animation Studies
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    • s.40
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    • pp.261-285
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    • 2015
  • Transmedia has become a significant theme in the media studies sector and an academic discourse itself since its wide diffusion through Henry Jenkins' seminar book Convergence Culture. The aim of this essay is to examine the authentic intention of the proposer to understand its profound meaning and value in the research discourse. Firstly, Transmedia has originated as a part of the convergence culture research context, which is an identical approach in the 'active audience' research tradition. Thus, a history of the research in terms of Birmingham Cultural Studies tradition and its implication is scrutinized. Secondly, in respect of Pierre Levy's 'collective intelligence' which made significant influence on the making of the Convergence Culture and Transmedia Storytelling, the meaing of the transmedia discourse is studied. Thirdly and finally, the implication of this concept as a critical theory or Critique in the Cultural Studies tradition, which has highlighted the importance of the revelation of the binary oppositions and structures of dominance/resistance, with the interpretation of the role of the collective intelligence idea in the transmedia discourse, is studied.