• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.559-564
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• 1998

The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively.

• Journal of Korean Society of Forest Science
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• v.101 no.4
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• pp.635-639
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• 2012

The present study was initiated to find out the insect vector of mulberry dwarf phytoplasma. Among the collected insects from mulberry cultivation region, the sucking insects (Hishimonas sellatus, Bothrogonia japonica and Balclutha punctata) were selected for transmission of mulberry dwarf phytoplasma. Sucking insects, H. sellatus and B. punctata successfully transmitted phytoplasma to healthy seedling mulberry and periwinkle plants by inoculation-feeding. Typical dwarf symptom was observed in mulberry tree, but very slender twigs on stem and formatted small leaves were found on periwinkle plants, which are typical witches' broom symptoms. The phytoplasmas were detected in all samples exception sucking insects B. japonica using phytoplasma universal primer pair R16F2n/R2.

• Journal of the Korean Institute of Rural Architecture
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• v.9 no.2
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• pp.13-20
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• 2007

This study examined the present condition and characteristics, spatial composition of the Atarasimura through field studies about the Atarasimura of Miyashiro, Saitama pref. in Japan. The results of this study are as follows. First, Atarasimura is the citizen farm of Miyashiro which promotes regional activation, community consciousness and the interchange of residents. Second, cultivation area and the miscellaneous scrub transforms the area into a park-like one, protecting a natural environment and making a beautiful landscape. Atarasimura is expected to enhance the studying effect of the children and young people. Third, the direct sale of agricultural products leads to the increase of income and resident employment opportunities. Fourth, the plan of future regional facilities must be made within a legal system and a systematic program for region making. In addition, the system of rational participation and cooperation between administration and local residents is important.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.108-111
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• 2005

A field study was conducted to develop an economically suitable combination of organic and inorganic fertilizers for sugarcane cultivation in sandy acidic soil of a commercial sugarcane farm under Old Himalayan Piedmont Plain of Bangladesh. Results revealed that the treatment $T_4$ which received $75\%$ of Recommended fertilizers (N 120, P 35, K 100, S 25, Zn 2 kg $ha^{-1}$) + 10 Mg $ha^{-1}$ Rice mill ash + Mg $ha^{-1}$ press mud significantly produced higher sugarcane yield (72.34 Mg $ha^{-1}$) among all the treatments except $T_5$ having $100\%$ of Recommended fertilizers + 10 Mg $ha^{-1}$ Rice mill ash + 10 Mg $ha^{-1}$ press mud, which was identical. $T_4$ also gave the highest net economic benefit at Bangladesh Taka 15,920.47 per hectare from the added nutrient management. Thus, the integrated use with organic and inorganic fertilizer is highly essential for sustainable production of sugarcane in commercial farm of the region in Bangladesh, where it has been grown year after year.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.4
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• pp.237-244
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• 2013

Radar remote sensing is appropriate for monitoring rice because the areas where this crop is cultivated are often cloudy and rainy. Especially, Synthetic Aperture Radar (SAR) can acquire remote sensing information with a high temporal resolution in tropical and subtropical regions due to its all-weather capability. This paper analyzes the relationships between backscattering coefficients of rice measured by RADARSAT-2 SAR and growth parameters during a rice growth period. We examined the temporal variations of backscattering coefficients with full polarization. Backscattering coefficients for all polarizations increased until Day Of Year (DOY 222) and then decreased along with Leaf Area Index (LAI), fresh weight, and Vegetation Water Content (VWC). Vertical transmit and Vertical receive polarization (VV)-polarization backscattering coefficients were higher than Horizontal transmit and Horizontal receive polarization (HH)-polarization backscattering coefficients in early rice growth stage and HH-polarization backscattering coefficients were higher than VV-polarization backscattering coefficients after effective tillering stage (DOY 186). Correlation analysis between backscattering coefficients and rice growth parameters revealed that HH-polarization was highly correlated with LAI, fresh weight, and VWC. Based on the observed relationships between backscattering coefficients and variables of cultivation, prediction equations were developed using the HH-polarization backscattering coefficients.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.126-132
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• 1978

To examine the effects of P.E. film and rice straw mulching on fall grown potato(Shimabara cultivar) in the middle region of Korea, sprouted seed tuber pieces were transplanted with six kinds of mulching treatments on 24th July and harvested on 25th October. P.E. film mulching from transplanting resulted the perfect rot of transplanted seed pieces due to the excessive high soil temperature (noon soil temperature of about 4$0^{\circ}C$ continued until the middle of August). P.E. film mulching from 15th September to harvest increased the marketable tuber yields by 35% due to the raising of soil temperature by 1-3$^{\circ}C$ and maintaining of available soil moisture in dry season. Rice straw (whole or cut in 10cm length) mulching lowered the soil temperature in hot season and raised it in cool season and maintained available soil moisture in dry season. Therefore better emergence, growth and yields were induced. Effects of whole straw, whole growing season and thicker mulching were greater than cut straw, half growing season and thinner mulching. 6cm mulching of whole or cut straw during whole growing season resulted the increasing of marketable tuber yields by 56 or 48%.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.363-370
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• 2012

Some physicochemical characteristics like nutritional values, sugar content, and fatty acid composition (FAs) in fruits of four papaya (Carica papaya L.) cultivars ('Red Lady', 'Sunrise Solo', 'Tainung', and 'BH-65') grown under plastic greenhouse conditions in the Mediterranean region of Turkey were evaluated. The chemical characteristics, except acidity in the fruits, significantly varied among the cultivars. Nitrogen (N) was the most abundant mineral in all papaya cultivars and ranged from $0.80\;g{\cdot}100\;g^{-1}$ in 'Red Lady' to $1.28\;g{\cdot}100\;g^{-1}$ in 'BH-65' in fresh weight. Potassium (K) and calcium (Ca) were found the highest amount in dry weight. Glucose and fructose were identified as the main sugars in all cultivars. Sucrose was in trace amounts in the three cultivars, but not detectable in cultivar 'Red Lady'. Twenty-five FAs were detected in the papaya fruits. Polyunsaturated fatty acids (PUFAs) were found to be in a highest amounts compared to the saturated fatty acid (SFAs) and monounsaturated fatty acids (MUFAs). Palmitic (C16:0), oleic (C18:1), and linoleic (C18:2n6) acids were the major fatty acids detected in all cultivars. The results of this study implied that dietary intake of papayas may supply substantial nutrient components necessary for human health.

• Hwankyungkyoyuk
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• v.11 no.2
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• pp.14-25
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• 1998

The Quality of human living depends on the environmental quality of the region sustaining the life. The environmental deterioration of the modern society is due to mechanical environmentalism. For the better quality of the life, The changes of recognition and attitude on the environments are required. These changes of mind are also important in environmental education for teenagers. The 7th national curriculum, officially anounced December 1998, focuses on the change of attitude to environments and practical behavior in real life for “Environments”, the environmental education curriculum in middle school. Basic elements of the curriculum are cultivation of the pro-environmental thinking, multi-levelling of teaching materials and methods, and encouraging of student participating activity. Actually, the curriculum construction is composed of stepped-levelling of teaching and learning, reasonable contents volume, encouraging of student practice, and suggesting of evaluation standards of textbook writing. Three main subjects of environmental education for middle school consist of (1) man and environment, (2) recognition of environmental problem, and (3) protection activity for environment. Methodology of environmental education can include multi-disciplinary approaches, variable teaching methods, and continuing evaluation of student practice and participation attitude. Environmental education for teenagers relating to the 7th national curriculum focuses on recognition of the environmental problems and practice activity in daily life. The recognition includes considering relationship of human life to environment, solving environmental problems in regional context, and development of comprehensive understanding concept of the environments. For the practice education, variable teaching methods, such as field survey and application of multi-media, are needed.

• The Journal of Fisheries Business Administration
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• v.45 no.2
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• pp.1-13
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• 2014

This study analyzes patent trends regarding farming-related technologies such as breeding or nursery technology, production systems, and new cultivation methods in order to provide objective patent information about the trends of R&D on marine products' proliferation or farming. Presently, in Korea, we try to secure marine living resources through the formation of artificial resources instead of environmental improvement policy or resource management policy. However, this is not about eliminating the causes of marine environment destruction but just the policy for improvement, so it is needed to revise the related policy with more ultimate measures. And it is necessary to develop technology that can secure the rights by integrating patent application strategies from the stage of R&D for medicine that can be utilized in chief farming countries and provide intensive support for the research centering around strategic varieties for farming in each region as well as strategic national farming varieties afterwards. Also, the analysis shows that it is urgent to make applications overseas based on domestic applications in order to develop technology that can be commercialized in not just Korean but overseas market as well.

• Journal of Environmental Science International
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• v.21 no.10
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• pp.1255-1263
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• 2012

Batch cultivations were performed to evaluate the influences of the initial pH condition on mesophilic and thermophilic acidogenic fermentation with food waste recycling wastewater. In both conditions of mesophilic and thermophilic fermentation, TVFAs production rates were maximized at the initial pH 7 condition as 0.15 and 0.23 g TVFAs/L hr, respectively. And pH was also maintained stably between 6 and 7 during 72hr acidogenic cultivation at both conditions. However, predominant VFA components were different according to reaction temperature conditions. In mesophilic condition, propionic acid which has low conversion efficiency to methane was accumulated up to 1,348 mg/L while acetic and butyric acid were predominant in thermophilic condition. Therefore, thermophilic acidogenic fermentation was superior for the effective VFAs production than mesophilic condition. From the DGGE analysis, the band patterns were different according to the initial pH conditions but the correlations of the each band were increased in similar pH conditions. These results mean that microbial communities were certainly affected by the initial pH condition. Consequently, the adjustment of the initial pH to neutral region and thermophilic operation are needed to enhance acidogenic fermentation of food waste recycling wastewater.