• 한국식품영양과학회지
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• 제23권5호
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• pp.845-848
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• 1994

The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ehtanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example , the acetic acid production increased proportionally by the increasing of initial concentration was higher that 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking than static cultivation by about 10 days with 150 rpm shaking speed. Acetic acid production with shaking cultivation was faster the static cultivation by abot 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor , the optimum fermentation condition was 700 rpm of agitation speed and 5L/min air flow rate in 3L culture medium .

• 한국버섯학회지
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• 제6권3_4호
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• pp.146-149
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• 2008

Pleurotus pulmonarius usually grows on the dead wood of deciduous trees in mid summer to early fall. The pileus color is white to tan and fruiting body is generally smaller than P. ostreatus. This study was carried out to investigate the characteristics of cultivation and consumer acceptance of P. pulmonarius. The period of incubation and primordial occurrence were required about 33days, 5days in bottle cultivation repectively, and the yield was 170g/850cc bottle. In bed cultivation, the period of primordial occurrence was similar to that of bottle cultivation and the yields was $35.7{\sim}46.2kg/3.3m^2$. Taste and favor of P. pulmonarius were superior to P. oostreatus., but the appearance, color and shape, was slightly inferior to P. ostreatus.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.99-99
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• 2022

This study was carried out to select the excellent silage maize varieties customized for paddy cultivation that is vulnerable to lodging and waterlogging in the central and region. Ten varieties (Kwangpyeongok, P3394, etc.) were sown with 3 replicates in Suwon, Gyeonggi-do. It was sown twice in April and June of each year in 2020-2021 and harvested during the yellow ripe stage suitable for silage. The number of days to flowering stage in lowland condition increased up to 11 days compared to that in upland condition. In April seedling, varieties that showed a relatively small decrease in biomass production and total digestible nutrients (TDN) under lowland condition compared to upland condition were Gangdaok and Kwangpyeongok in 2020, and Dacheongok, Gangdaok and Kwangpyeongok in 2021. Kwangpyeongok, Gangdaok, and Sinhwangok showed relatively higher biomass production and TDN than the other varieties under lowland compared to upland in both 2020 and 2021. Our results suggest that Kwangpyeongok and Gangdaok are suitable silage maize varieties for lowland cultivation in the central region of Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.198-198
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• 2017

Cultivated varieties of sweet potato were from dry texture type to tender texture type on the basis of consumer preferences. There are many differences in the quantity of sweet potato, starch content, pigment, and sugar content depending on the cultivation season and area, even in the same variety. Therefore, in this study, we attempted to establish optimum time of harvesting through growth characteristics and variation in component like starch, sugar, polyphenol and flavonoid. Four sweet potato varieties were used in this experiment. Among them, Jinhongmi (JHM) & Yulmi (YM) were as dry texture type and Pungwonmi (PWM) & Hogammi (HGM) were as tender texture type. Sweet potatoes were transplanted on 23 May, 2016 and were investigated storage root weight and component contents every 20 days from 60 days to 120 days and surveyed yield at 110, 120, 130 days after transplantation. Result revealed that storage root weight of YM, JHM, and HGM were 30.1, 38.9, 20.8 g respectively in 60 days after transplanting. Storage roots of PWM gerw faster with the weight of 88.2 g. In 120 days after transplanting, storage root weight varied from 88.3 to 118.7 g, HGM was the smallest, and PWM was the largest. Sugar contents of sweet potato ranged from 21.0 to $23.8Brix^{\circ}$ in 60 days after transplanting and from 27.5 to $30.78Brix^{\circ}$ in 120 days after transplanting. In particular, the sugar content of HGM was the highest over $30Brix^{\circ}$ after 80 days. The starch content of dry texture type (YM, JHM) increased from 15.5% to 20.4% and tender texture type (PWM, HGM) increased from 11.0% to 17.3%. Starch content tended to be high in dry type sweet potatoes. The content of polyphenol and flavonoid were highest in 60 days after transplanting and was reduced according to cultivation period. The total yield of PWM was high as 3,154 kg/10a and large storage root of over 250 g accounted for 47.4% in 110 days after transplanting. Storage root (YM, JHM, HGM) of 81~150 g accounted for 34.9% ~ 43.2% in 120 days after transplanting. These are the most marketable. Because consumer in Korea prefers small, round and about 100g size sweet potato. The ratio of large storage root (over 250 g) were increased in all varieties at 130 days after transplanting. Therefore, it is considered appropriate to harvest PWM at 110 days and YM, JHM, HGM at 120 days after transplanting, which planted in late May.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권6호
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• pp.368-372
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• 2003

The growth and amino acid contents of the cyanobacterium, Spirulina platensis strain NIES 46, were investigated using ammonium, nitrate, nitrite, or urea as the sole nitrogen source in a batch culture. Chlorophyll a concentration was highest at 2,096$\mu\textrm{g}$/L in the nitrate group after 10days of cultivation, while the dry weight of S. platensis was highest at 4.5g/L in the ammonium group after 30days of cultivation. The total amino acid content was highest at 174mg/g dry weight of S. platensis in the urea group at the end of the cultivation period, yet the amino acid patterns for S. platensis were similar for all the experimental groups. Therefore, it seemed that the growth and amino acid composition of S. platensis varied depending on the type of nitrogen sources, while the amino acid patterns were not changed. Also, the most efficient harvesting time for S. platensis seemed to be approximately 10 days after cultivation.

• 한국자원식물학회지
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• 제37권2호
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• pp.149-160
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• 2024

국내에서 감자는 주로 반찬이나 부식으로 이용해 왔지만 최근에는 칩이나 프렌치프라이 등 스낵이나 간식으로 이용이 증가하고 있다. 가공원료용 생감자를 사용하여 감자를 가공하는 것에 대한 수요는 높지만 감자를 2기작으로 특히 가을에 재배하였을때 가공품질이 어떻게 달라지는 지에 대한 연구는 부족한 실정이다. 따라서 본 연구는 봄감자 재배기간을 달리하여 가을감자를 재배하였을 때 생육과 수량 및 가공품질에 미치는 영향을 구명하고자 수행하였다. 봄감자 파종 후 수확을 70일, 80일, 90일, 100일 총 4번에 거쳐 하였으며, 이를 활용하여 각각 강릉과 서천 지역에서 가을재배를 수행하였다. 지상부 출현율은 봄감자 재배기간이 짧은 씨감자를 가을재배 했을 때 높아지는 경향이었다. 경장은 봄재배 기간이 80일 및 90일인 씨감자를 강릉 지역에서 가을재배했을 때 47.2~48.9로 다른 처리에 비해 컸다. 재배기간에 따른 경수와 엽색은 통계적 유의성이 없었다. 수확시기에 따라 괴경수, 괴경무게, 수량 및 상서수량은 통계적 유의성이 없었으며 품종에 따른 차이는 뚜렷하게 나타났다. 괴경 무게는 강릉에서는 새봉 품종이, 서천에서는 은선 품종이 우수하였다. 전분 함량은 봄에 80일간 재배한 씨감자를 강릉 지역에서 가을재배 했을 때 7.9%로 함량이 높았으며, 서천 지역에서는 유의성이 나타나지 않았다. Glucose 함량은 재배기간에 따라 뚜렷한 차이를 보였는데, 봄감자 재배기간이 긴 씨감자를 가을재배에 이용할 경우 그 함량이 높아졌다. 감자를 2기작으로 재배 시 봄감자 재배기간이 괴경의 생육, 수량 및 가공품질에 미치는 영향에 대해 연구한 결과, 재배기간에 따른 차이는 크지 않았고, 품종에 따른 차이가 뚜렷하였다. 은선 품종은 가을재배 시 지상부 생육 및 수량이 뛰어났고, 가공품질은 새봉 품종이 우수하였다.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.716-721
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• 1999

It has been known that continuous cultivation of hairy root is difficult to maintain for a long period of time compared to the microbial and callus cultures. Chemostat cultivation was successfully carried out in order to economically produce a plant-based colorant, betacyanin, from red beet hairy root for more than 85 days in a 14-1 fermentor. The result from the chemostat cultivation was compared to those of the batch and fed-batch cultivations of red beet hairy roots. It was shown that hairy root reached its steady state within 50 days of the cultivation, and then maintained for about 25-30 days in a wide range of dilution rates. Total betacyanin production from the continuous process was also calculated to be 2.65g at 0.28(l/d) of dilution rate, compared to 0.196g from fed-batch cultivation. It was found that betacyanin production was a partially growth related process, yielding 0.376 mg/g-fresh wt. cell and $1.89{\times}10^{-5}$ mg/g-fresh wt. cell/d, with 0.92 of correlation factor in a partial growth-product model. It was also shown that the cell growth required was relatively large for maintenance amount of energy at a low dilution rate. The growth of hairy root was inhibited by high light intensity in following a photo-inhibition model. The growth parameters were estimated to be 0.3(l/d), $10.56kcal/\textrm{m}^2/h$,{\;}and{\;}35.81kcal/\textrm{m}^2/h$ for the maximum specific growth rate, half saturation light intensity, and inhibition light intensity, respectively.

• 한국버섯학회지
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• 제13권2호
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• pp.92-96
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• 2015

팽이버섯 신품종 '금향'은 '갈뫼'와 수집 야생균주인 'CBMFV-33'으로부터 분리한 단포자를 교배함으로써 육성된 품종으로, 갓 색이 진한 미색을 나타내며, 재배기간이 짧다. 병재배 시 배양 기간은 23일, 초발이 소요일수는 7일, 생육일수는 17일로 총재배기간이 47일이 소요되어 대조품종 '갈뫼'에 비해 7일이 단축되었다. 갓 직경은 '갈뫼'에 비해 크고, 갓 색은 진한 미색을 나타냈다. 수량은 850 ml 병 당 162 g으로 '갈뫼' 대비 8% 증수되었다. 또한 야생 팽이버섯처럼 식미감도 우수하여, 차별화된 색과 맛으로 백색 팽이버섯의 틈새시장 공략에 유리할 것으로 판단된다.

• 한국초지조사료학회지
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• 제18권3호
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• pp.195-204
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• 1998

This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

• 한국미생물·생명공학회지
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• 제42권1호
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• pp.67-72
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• 2014

A new substrate, breadcrumbs, was investigated for biomass accumulation, the pH of the cultural broth, the formation of primary metabolites such as the proteins and endopolysaccharides of Schizophyllum commune 1768 and Trametes versicolor 353, as well as its bioconversion efficiency. The results showed that S. commune gives more mycelial mass ($23.96{\pm}0.8g/l$) and in a shorter period (4 days) than T. versicolor ($15.76{\pm}0.5g/l$ in 5 days). The pH values changed from the initial 6.1 to 3.6 in S. commune cultural broth and to 4.4 in T. versicolor cultural broth. Maximal endopolysaccharide content in the mycelia of S. commune and T. versicolor were 7.13% and 6.42%, correspondingly. Crude protein content in S. commune mycelium was 18.83 % on the 4th day of cultivation, and 20.03%, in the mycelium of T. versicolor, on the 6th day of cultivation. Kinetic parameters for the quantitative estimation of cultivation efficiency were calculated for biomass, endopolysaccharide, and crude protein concentrations.