• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.96-102
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• 1998

Primary hepatocytes of small animals such as rat and rabbit were often used for the study of extracorporeal liver support systems. Freshly isolated rat hepatocytes form spheroids within tow days when cultivated as suspension in spinner vessels. These spheroids showed enhanced liver specific functions and more differentiated morphology compared to hepatocytes cultured as monolayers However, shear stress caused by continuous agitation deteriorated spheroids gradually. In this work we immobilized spheroids to prolong liver specific activities. First, hepatocyte spheroids were suspended in collagen solution containing calcium chloride and then dropped into alginate solution. A thin layer of calcium alginate was formed around the droplet and then was removed after the inner collagen was gelled by treatment of sodium citrate buffer. Spheroids embedded in collagen-gel bead maintained liver specific functions such as albumin secretion rate longer than hepatocyte spheroids exposed to shear stress. Therefore, we suggest that this immobilization technique may offer an effective long-term hepatocyte cultivation and facilitase the development of a bioartificial liver support device.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1471-1477
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• 2012

Carotenoids produced by non-photosynthetic bacteria protect organisms against lethal photodynamic reactions and scavenge oxygenic radicals. However, the carotenoid produced by Gordonia alkanivorans SKF120101 is coupled to reducing power generation. SKF120101 selectively produces carotenoid under light conditions. The growth yield of SKF120101 cultivated under light conditions was higher than that under dark condition. In the cyclic voltammetry, both upper and lower voltammograms for neutral red (NR) immobilized in intact cells of SKF120101 were not shifted in the condition without external redox sources but were commonly shifted downward by glucose addition and light. Electric current generation in a biofuel cell system (BFCS) catalyzed by harvested cells of SKF120101 was higher under light than dark condition. The ratio of electricity generation to glucose consumption by SKF120101 cultivated in BFCS was higher under light than dark condition. The carotenoid produced by SKF120101 catalyzes production of reducing power from light energy, first evaluated by the electrochemical technique used in this research.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2001.12a
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• pp.1-4
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• 2001

The classification performance of Artificial Neural Network (ANN) and RBF-NN was compared for Landsat TM image. The RBF-NN was validated for three unique landuse types (e.g. Mixed landuse area, Cultivated area, Urban area), different input band combinations and classification class. The bootstrap resampling technique was employed to estimate the confidence intervals and distribution for unit load, The pollutant generation was varied significantly according to the classification accuracy and percentile unit load applied. Especially in urban area, where mixed landuse is dominant, the difference of estimated pollutant load is largely varied.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.6
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• pp.1-15
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• 2014

A dredging on erosion control dam has been enforced without evaluation the factors that affect the dredging. In addition, there is the negative effect much more than positive effect by dredging on erosion control dam. Therefore, this study was carried out to develop evaluation indicators and to suggest fieldbook in order to determine whether sand deposits at erosion control dam should be dredged up or not. The most important six evaluation indicators that can decide to dredge up at erosion control dam were obtained from three round delphi technique and were selected in the following order: the current sand deposit ratio(0.339), existence of cultivated land and house downstream(0.276), the slope of streambed(0.162), the amount of movable soil and gravel(0.118), the history of any disasters(0.063), the basin area(0.043). The weighted score for each evaluation indicator were acquired from AHP analysis with respect to the degree of importance and then the modified weighted score for actual measurements were classified as three categories: large(2.53), medium(1.60) and small(1.01). Based on delphi technique, erosion control dam dredging evaluation fieldbook introduced the four evaluation indicators out of the total six evaluation indicators and two low effected evaluation indicators were excluded. This results showed that the values for reliability analysis and consistency ratio were acceptable.

• YAKHAK HOEJI
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• v.17 no.1
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• pp.21-26
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• 1973

The constituents of Korean cannabis were studied comparatively with foreign orgins. Especially, tetrahydrocannabinol(THC), the active constituents of cannabis were analyzed by the technique of thin layer chromatography and colorimetry. The results are as follows ; (1) THC content in Korean cannabis is comparatively higher than that in foreign samples. (2) THC is contained most abundantly in male flowers, abundantly in female tops and leaves and some in barks. (3) There is a tendency that the THC content increase gradually with growth of plants, being highest during unripe nad decrease with maturity of the tops. (4) Korean cannabis contains THC, cannabinol, cannabidiol, cannabidiolic acid and other cannandiolic compounds. Distribution of chemical components of Korean cannabis, compared with those of foreign ones, is remarkably different. (5) The THC content of Spanish cannabis cultivated tentatively in Korea is similar to that of Korean cannabis.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11a
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• pp.185-191
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• 2003

Walled town was located on the axis connecting symbolic places, which was a feng-shui lucky place of a mountain sits to the rear and a body of water sits to the front. It represents environmental development of cultural space by blend of folk belief, religion, social system, administration facilities and living culture buildings. Therefore it is sustainable cultural view connected of formed or formless religious view and artificial view(wall, government buildings, living houses, and cultivated land so on) in a nature landscape. Environmental design technique, enlarging its meaning and value of living to mental level was founded from space composition and settlement, which was constructed organized space of government and residence area of walled town within the wall for looking for lucky place(from best, better, and good places) in order to construct ecological network (天+地+人, 山＋水＋方位＋人, 地理＋生利＋山水＋人心) by outlook of space and settlement.

• Animal cells and systems
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• v.10 no.3
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• pp.163-167
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• 2006

Reverse-Phase High-performance Liquid Chromatography (RP-HPLC) based technique is one of the most sensitive techniques to detect the antibiotics present in honey. In the southern part of Tamilnadu, India, majority of the farmlands are occupied by plantations such as coconut, banana and rubber. A variety of antimicrobial compounds and antibiotics, which have been reported in pollen, nectar and other floral parts of the plant, gets accumulated in honey through honeybees (Apis mellifera). We have collected the nectar samples from banana (Musa paridasiaca) and rubber (Ficus elastica) flowers and the honey from honey hives of banana and rubber cultivated areas. The extracted nectar and honey samples are subjected to RP-HPLC analysis with authentic antibiotic standards. Nectar and honey samples showed 4-17, 11-29 ${\mu}g/kg$ of streptomycin, 2-29, 3-44 ${\mu}g/kg$ of ampicillin and 17-34, 26-48 ${\mu}g/kg$ of kanamycin respectively.

• Journal of Plant Biotechnology
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• v.49 no.2
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• pp.107-117
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• 2022

Increasing soil salinity is one of the global challenges that the agriculture sector in Egypt has been facing; 33% of the cultivated land in Egypt, which includes merely 3% of the entire land area, is already salinized. The present review sheds light on the role of fungal biopriming, a technique in which hydrated seeds are inoculated with beneficial fungal flora, in mitigating the deleterious influence of NaCl tension. Endophytic fungi were recognized to be able to interact with several plant species, markedly contributing to the mitigation of NaCl stress in these plants, such that some plants get impoverished to their absent associated microbes under stressful conditions.

• Korean Journal of Remote Sensing
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• v.38 no.6_1
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• pp.1007-1014
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• 2022

Compact Advanced Satellite 500 (CAS500) can be used for various purposes, including vegetation, forestry, and agriculture fields. It is expected that it will be possible to acquire satellite images of various areas quickly. In order to use satellite images acquired through CAS500 in the agricultural field, it is necessary to develop a satellite image-based extraction technique for crop-cultivated areas.In particular, as research in the field of deep learning has become active in recent years, research on developing a deep learning model for extracting crop cultivation areas and generating training data is necessary. This manuscript classified the onion and garlic cultivation areas in Hapcheon-gun using PlanetScope satellite images and farm maps. In particular, for effective model learning, the model performance was analyzed according to the proportion of crop-cultivated areas. For the deep learning model used in the experiment, Fully Convolutional Densely Connected Convolutional Network (FC-DenseNet) was reconstructed to fit the purpose of crop cultivation area classification and utilized. As a result of the experiment, the ratio of crop cultivation areas in the training data affected the performance of the deep learning model.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.643-653
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• 2022

This study investigated which nutrient solution is suitable for growth and secondary metabolite contents when three different vegetable plants are grown simultaneously in one hydroponic cultivation bed. Seeds of pak choi (Brassica compestris L. ssp chinsensis), red mustard (Brassica juncea L.), and arugula (Eruca sativa Mill.) were sown in the shape of a triangle in three places on rockwool cubes. The rockwool cubes were placed in semi deepflow technique (semi-DFT) hydroponic systems in a rooftop greenhouse after three weeks of growth as seedlings then cultivated with four different nutrient solutions, Korea Horticultural Experiment Station (KHE), Hoagland, Otsuka-A, and Yamazaki, at the rooftop greenhouse for two weeks. The leaf area of pak choi cultivated in Otsuka-A was the largest but SPAD values, leaf area, and fresh weight of arugula were highest with KHE treatment. The total glucosinolate (GSL) content of pak choi was 151.7% higher in KHE than in Hoagland, and there was no significant difference in Yamazaki and Otsuka-A treatments. The total GSL content of red mustard was 34.6 μmol·g^-1 in Hoagland, and it was 32.6% higher in Hoagland than in Yamazaki. Total GSL content of arugula was 57.5% higher in Yamazaki and Hoagland nutrients than in KHE and Otsuka-A nutrients solutions. The total GSL content of three plants grown with KHE was 40.7% higher than with Yamazaki, and the other nutrient solutions did not show significant differences. Therefore, KHE nutrient solution is considered suitable for nutrient solution composition for the cultivation of three different Brassicaceae crops in a single hydroponic cultivation system.