• 한국식품영양학회지
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• 제33권1호
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• pp.91-97
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• 2020

The purpose of this study was to provide the basic data of butternut squash (Cucurbita moschata) according to the drying methods. The proximate composition and chromaticity of freeze-dried and hot air-dried Cucurbita moschata powders were evaluated, and the antioxidant effects of these ethanolic extracts were analyzed by DPPH, ABTS, FRAP, reducing power activity, total phenolic, and flavonoid content. As a result, Cucurbita moschata was revealed to have high carbohydrate and crude protein contents, while crude ash and crude fat contents were relatively low. Also, it was confirmed that the hot air-dried Cucurbita moschata had lower L, a, and b values than the freeze-dried Cucurbita moschata. Additionally, as a result of analyzing the antioxidant activity of Cucurbita moschata ethanolic extracts, hot air-dried Cucurbita moschata ethanolic extract showed significantly superior antioxidant activity than freeze-dried Cucurbita moschata ethanolic extract. Also, hot air-dried Cucurbita moschata ethanolic extract was revealed to have a higher polyphenol content, but slightly lower flavonoid content than freeze-dried Cucurbita moschata ethanolic extract. Based on the data from this study, further experiments on Cucurbita moschata material are necessary.

• 한방비만학회지
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• 제9권2호
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• pp.57-63
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• 2009

In recent year, We are concerned about anti-aging, disease-protection, long-life, many methods are used in solving this problem. And Those are related with antioxidative ability. Recently, We heard that Cucurbita moschata Duch. has anti-hypertensive effect and good for health. So I let made a experiment for this result of the anti-oxidant effect of Cucurbita moschata Duch. used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity and Bovine serum albumin(BSA). The results of this study were as follows: We measured level of DPPH radical scavenging activity. And we obtained results that the ability of DPPH radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $20\;mg/m{\ell}$. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $10\;mg/m{\ell}$. When we inspected antioxidation with Bovine serum albumin(BSA). And we obtained that antioxidative ability was increased after$2.5\;mg/m{\ell}$ of Cucurbita moschata Duch.. So I guess that Cucurbita moschata Duch. has effect of antioxidative ability. Hereafter we need differential experimental methods for evidence of antioxidative effect on Cucurbita moschata Duch.

• Preventive Nutrition and Food Science
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• 제6권2호
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• pp.133-135
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• 2001

The edible part of cucurbita moschata Duch, which is commonly used as a Korean traditional medicine as well as a popular food source, was studied to isolate anti-complementary substance. Extracts of Cucurbita moschata Duch showed significant anti-complementary activities on the classical pathway of the complement system. Especially, the ripe Cucurbita moschata Duch had more activity than that of the complement system. Especially, the ripe Cucurbita moschata Duch had more activity than that of the green one in terms of the overall anti-complementary activity. Among the extracts of various organic solvents of the ripe Cucurbita moschata Duch, chloroform and ethyl-acetate extracts, which are non-polar solvent extracts, showed the strongest activities. These results suggest that the major difference in the solvent extraction for the anti-complementary substances depends on the change in the chemical composition such as the fatty acid with the degree of ripening.

• Preventive Nutrition and Food Science
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• 제6권4호
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• pp.244-246
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• 2001

Angiotensin converting enzyme (ACE) inhibitor acts on the inhibition of ACE and causes a decrease in blood pressure. There have been several reports on screening of ACE inhibitors from natural food products and protein hydrolysates of various food sources. Ripe Cucurbita moschata Duch has been used as an oriental medicine in Korea. To isolate ACE inhibitors, crude water extracts of the edible portion of ripe Cucurbita moschata Duch were obtained after heating in water at 95$^{\circ}C$ for 2 h. Crude extracts were then filtered using PM-10 and YM-1 membranes. The membrane-filtered solution was loaded onto Sephadex G-15 column equlibrated with a phosphate buffer. Among the four major fractions of gel permeation chromatography, the second fraction had the highest inhibitory activity of 65%. Further purification of the fraction using reversed-phase HPLC with a $C_{18}$ column produced ACE inhibitors, which were identified as a mixture having molecular mass of 222 and 273 by Tandem mass spectrometry.

• Applied Biological Chemistry
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• 제42권3호
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• pp.267-270
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• 1999

• Archives of Pharmacal Research
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• 제25권4호
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• pp.438-440
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• 2002

A new unsaturated hydroxy fatty acid was isolated from the leaves of Cucurbita moschata through repeated silica gel column chromatography and chemical methods. The structure of the new fatty acid was determined as 13-hydroxy-9, 11, 15-octadecatrienoic acid on the basis of several spectral data including 2D-NMR. The stererostructures of double bonds were determined to be 9Z, 11 E and 15E by coupling patterns of related proton signals in the $^1H-NMR$ and NOESY experiments.

• 한국식품영양과학회지
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• 제39권2호
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• pp.165-171
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• 2010

늙은 호박의 부위를 나누고 추출용매를 증류수, 50% EtOH, 100% EtOH로 달리하여 건물 중량의 25배에 해당되는 부피(w/v)일 때, 추출물의 생리활성 및 항산화 효과를 측정하였다. 추출물의 수율을 측정한 결과 추출용매를 증류수로 사용한 추출물들이 가장 높았으며, 특히 섬유질 추출물이 14.62%로 가장 높게 나타났다. 전자공여 작용의 경우 50% EtOH로 추출한 섬유질 추출물이 52.37%의 높은 전자 공여능을 보였다. SOD 유사활성을 측정한 결과에서는 100% EtOH에 의해 추출된 과육 추출물이 42.48%로 다른 추출물보다 높은 유사활성을 나타냈다. 총 폴리페놀 함량의 경우 50% EtOH로 추출한 섬유질 추출물이 121.49 mg%로 가장 높게 나타났다. 아질산염 소거능 측정 결과에서 모든 추출물이 pH 1.2에서 소거능이 가장 높게 측정되었다. Tyrosinase 활성 저해능의 경우 모든 과육 추출물이 11.36~19.14%로 가장 높게 나타났으나 비교물질인 L-ascorbic acid에 비해 매우 낮은 저해능을 보였다. ACE 저해활성에서도 전자공여능과 페놀함량 측정결과와 같이 50% EtOH로 추출한 섬유질 추출물이 가장 높게 나타났다.

• 한국식품과학회지
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• 제43권2호
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• pp.195-199
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• 2011

본 연구에서는 늙은호박(Cucurbita moschata Duch.)의 부위별 항산화 효과 및 생리활성을 평가하기 위하여 잎과 과피, 과육과 씨 네 부분으로 나누어 일반성분을 측정하고, 환류 추출과 감압농축 및 동결건조를 통하여 분말을 얻은 후 실험에 사용하였다. 호박의 부위별 총 폴리페놀 함량은 잎이 $29.62{\pm}0.88mg$ GAE/g DM로 가장 높게 측정되었다. 플라보노이드 함량도 잎이 가장 높고 과피, 과육, 씨의 순서로 측정되어 폴리페놀 함량과 같은 경향을 보였다. DPPH radical 소거 활성과 ABTS radical 소거 활성에서 SC50값을 측정하였을 때는 잎에서 가장 높은 소거 활성을 나타내었다(DPPH: $1.27{\pm}0.14$, ABTS: $2.28{\pm}0.05$). FRAP assay를 이용한 환원력을 실험한 결과에서도 잎에서 가장 높은 환원력이 관찰되었다. 한편, 생리활성적인 측면 중 tyrosinase 효소 억제 활성 능력은 대조군인 kojic acid와 비교하였을 때 같은 농도인 0.1 mg/mL에서 20.70%이었던 반면 실험군인 잎은 49.58%, 과육은 40.20%을 보였고, 과피는 41.30%, 씨는 36.79%가 관찰되어 모두 이보다 높은 수치를 나타냈으므로 kojic acid 보다 효과가 있는 것을 확인할 수 있었다. 최종당화산물(AGEs) 생성억제 효능에서는 잎(65.85%), 과피(50.14%), 과육(13.72%), 그리고 씨(11.18%)의 순서로 관찰되었다. ${\alpha}$-Glucosidase 저해활성은 씨 부분의 저해활성이 63.58%로 67.28%를 나타낸 대조군 0.06 mM acarbose에 근접한 수치를 나타내었다.

• 식물병연구
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• 제11권2호
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• pp.106-114
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• 2005

To breed powdery mildew resistant varieties, 104 accessions of Cucurbita spp. germplasm were evaluated. Powdery mildew resistance and horticultural characteristics of the accessions were investigated in the field conditions. C. martinezii was selected for high resistance to powdery mildew in the field. The growth of powdery mildew pathogen and response of leaf surface tissue to the pathogen were observed after artificial inoculation to resistant C martinezii and susceptible C. moschata cv. 'Wonye No.402'. Mycelium growth was very clearly different between resistant C. martinezii and susceptible C moschata cv. 'Wonye No. 402' at 5 days after inoculation at the adult stage. Interspecific hybridization was attempted to transfer powdery mildew resistance gene(s) from C. martinezii to C. moschata. A single dominant gene action was postulated as deter mining powdery mildew resistance at the seedling stage. But for the effective selection of powdery mildew resistant individuals, it was established that adult plant resistance should be confirmed in the field conditions after the seedling test. Through the seedling and field tests, C. moschata lines resistant to powdery mildew and superior to horticultural characteristics, were selected after 2 backcrossing and 3 selfing generations. The fruit characteristics of newly developed lines were similar to those of the recurrent parent, 'Jaechenjarae'.

• Applied Biological Chemistry
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• 제40권5호
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• pp.375-379
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• 1997

리보즘불활성화 단백질(Ribosome-inactivating protein, RIP)을 생성하는 식물을 탐색하여 그중 호박$(Cucurbita\;moschata\;D_{UCHESNE})$ 잎에서 ammonium sulfate 침전, DE 52-Cellulose, S-Sepharose, FPLC Superose 12 HR, FPLC Mono-S column chromatography에 의하여 ribosome-Inactivating 활성이 있는 단백질(PR 1, PRIP 2)을 분리하였다. 정제된 단백질의 분자량은 SDS-PAGE에서 약 31,000과 30,500인 염기성 단백질로서, 특히 PRIP 1은 열에도 안정하여 $50^{\circ}C$에서 30분간 처리한 경우에도 활성이 유지되었다. 이 단백질들의 ribosome-inactivating 활성을 in vitro translation system에서 측정한 결과 50% 활성저해농도 $(IC_{50})$는 PRIP 1은 0.82nM, PRIP 2은 0.79 nM이었다. PRIP 1과 PRIP 2의 N-말단부분의 아미노산 서열을 분석하여, 이미 밝혀진 리보즘불활성화 단백질들과 아미노산서열의 유사성을 분석해 본 결과, PRIP 1은 Luffa cylindrica에서 분리된 Luffin B 및 Trichosanthes kirilowii Maximowicz에서 분리된 Trichokirin과, PRE 2은 Momordia charantia에서 분리된 Momordin II 및 MAP 30과 유사성이 매우 높았다.