• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.325-325
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• 1997

No Abstract, See Full Text

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.42-42
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• 2019

• The Korean Journal of Food And Nutrition
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• v.8 no.3
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• pp.159-164
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• 1995

The test possibility of L-ascorbic acid(AsA) assay by ascorbate oxidase(A.O) solution obtained from cucumber was estimated. The results obtained were summarized as follow. The difference of absorbance before and after oxidation of AsA by A.O solution was proportional to the AsA concentration. Iso- AsA compared to same concentration of AsA was 97% response. Therefore, the 0 specificity on Iso- AsA a was deficient. On the sucrose, glucose, fructose were coexisted from 5 times, 500 times of AsA concentration, none of them were affected by any level of AsA concentration. In the case of EDTA, it was not nearly affected that EDTA was coexisted 50 times of AsA concentration, but EDTA Inhibited considerably from 500 times of AsA concentration. The effect of citric acid on the AsA concentration was not in the same level of AsA, however, it was slightly inhibited from 5 times, 50 times of AsA concentration and 500 times of AsA inhibited remarkably As known L-AsA was catalycally autooxidated by Cu2+ and Fe2+. They were not nearly affected In the above 0.01M level on the Ash assay. However, Fe3+ ion was neither nearly affected in the 0.001M level nor In the above 0.01M level on the AsA assay.

• Korean Journal of Food Science and Technology
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• v.29 no.2
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• pp.314-319
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• 1997

The objectives of this study were to examine the effect of storage time, temperature, pH, and NaCl concentration on hydroperoxide lyase and lipoxygenase activities, and to establish important informations to the production of typical cucumber flavor. Conditions affecting lipoxygenase and hydroperoxide lyase would be important for cucumber flavor production. Maximum activity was observed at pH 5.0 for hydroperoxide lyase and pH 5.5 for lipoxygenase. Both enzymes were relatively stable at $40\;to\;50^{\circ}C$ for 6days storage time. Maximum activity of both enzymes was observed with 0.2 M NaCl at pH 5.0. Activities were stimulated with concentrations of NaCl from 0.05 to 0.2 M. Hydroperoxide lyase and lipoxygenase activities were decreased at concentration of NaCl greater than 0.2 M.

• Korean Journal of Plant Tissue Culture
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• v.28 no.1
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• pp.53-58
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• 2001

To develop herbicide-resistant cucumber plants (Cucumis sativus L. cv Green Angle) embryogenic suspension cultures were co-cultured with Agrobacterium tumefaciens strain LBA4404 carrying a disarmed binary vector pGA-bar. The T-DNA region of this binary vector contains the nopalin synthase/neomycin phosphotransferase Ⅱ (npt Ⅱ) chimeric gene for kanamycin resistance and the cauliflower 35S/phosphinothricin acetyltransferase (bar) chimeric gene for phosphinothricin (PPT) resistance, After co-cultivation for 48 h, embryogenic calli were placed on maturation media containing 20 mg/L PPT. Approximately 200 putatively transgenic plantlets were obtained in hormone free media containing 40 mg/L PPT. Northern blot hybridization analysis confirmed the expression of the bar gene that was integrated into the genome of five transgenic plants. Transgenic cucumber plants were grown to maturity. Mature plants in soil showed tolerance to the commercial herbicide (Basta) of PPT at the manufacturer's suggested level (3 mL/L).

• Research in Plant Disease
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• v.14 no.2
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• pp.134-137
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• 2008

A strain of Cucumber mosaic virus(CMV) was isolated newly from sweet pepper(Capsicum annuum var. angulosum) showing necrosis with large necrotic spots on fruits and vein banding with malformation on leaf at Cheongdo area in Gyeongsangbukdo. The new strain was designated as a CMV-NP and the shape of virus particles was isometric of 26 nm in diameter from the sweet pepper fruit by Dip method. The strain of CMV-NP was identified genetically by VC/RT-PCR. CMV-NP could infect systemically on the 9 indicator plants including Cucumis sativus, but it could infect locally on Chenopodim amaranticolor and C. quinoa. CMV-NP induced the specific symptoms of necrotic rings on the inoculated and the upper leaves of N. rustica and Tetragonia expansa. On Cucumis sativus, the large chlorotic ring and vein chlorosis were produced on the upper leaves. CMV-NP had no virulence on Datura stramonium.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.199-202
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• 2008

Rhizopus soft rot caused by Rhizopus stolonifer occurred on melon (Cucumis melo L.) in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, black and eventually rotted. The symptoms were infected from wounds at harvest time. The colony were white to light brown, formed much sporangiospores. The optimum temperature of the fungus was $30^{\circ}C$. Sporangia were globose or hemispheric, white at first and gradually to black, and $80{\sim}195\;{\mu}m$ in size. Sporangiophores were $12{\sim}24\;{\mu}m$ in width. Columella were hemispheric, and $70{\sim}90\;{\mu}m$ in size. Sporangiospores were irregular round or oval, brownish-black and $9{\sim}20\;{\times}\;6{\sim}8\;{\mu}m$ in size. On the basis of symptom, mycological characteristics and pathogenicity to melon, the causal fungus was identified as Rhizopus stolonifer (Ehrenb.) Vuill.. This is the first report of Rhizopus soft rot by R. stolonifer on melon in Korea.

• Applied Biological Chemistry
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• v.40 no.6
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• pp.572-576
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• 1997

Effect of anoxia treatment on alcohol fermentation in the placenta of oriental melons (Cucumis melo) at different developmental stages was studied. Results showed that fruits at the rapid growth stage (stage III) contained the lowest amount of acetaldehyde and ethanol as compared with fruits at other developmental stages. During anoxia treatment, a steady increase in ethanol content was observed in the placenta of oriental melons, regardless of their developmental stages, while the increment of acetaldehyde content was relatively small. Alcohol dehydrogenase in growing and maturing stage fruits showed increased activity with the maximum value at one day after the onset of anoxia treatment and then decreased gradually. An increase in the activity of pyruvate decarboxylase was also observed during anoxia.

• Korean journal of applied entomology
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• v.42 no.2
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• pp.119-124
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• 2003

The adult of the Cotton caterpillar, Palpita indica (Saunder) laid eggs more on mature leaves than on aged and developing leaves. They laid more on leaves than on petiole and stem, and more on the adaxial than on the abaxial surface side of the leaves. Larvae of the Cotton caterpillar showed their preference in the order of the cucumber (Cucumis sativus), gourd (Lagernaria siceraria), watermelon (Citrullus lanatus) ＞ oriental melon (Cucumis melon L. var makuwa), wax gourd (Benincasa hispida), melon (Cucumis melo), star cucumber (Sicyos angulatus) ＞ sponge cucumber (Lufa cylindrica), cotton (Gossypium indicum). There were no distinct differences among varieties and lines that were collected and hybridized at the Gochang Watermelon Experiment Station in Korea. There was a positive relationship between the leaf area and the degree of damage by the Cotton caterpillar. The feeding amounts of the Cotton caterpillar excrement were gradually increased to 16-18 days after hatching, after that the amounts of excrement were rapidly decreased.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.125-129
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• 1998

Cucumber (Cucumis sativus L.) plants were regenerated through organogenesis and somatic embryogenesis in cotyledon and hypocotyl cultures. The shoots were efficiently formed on the basal region of cotyledons cultured on MS medium containing 1.0㎎/L zeatin and 0.1㎎/L IAA in all cultivars used. Embryogenic calli were formed on hypocotyl segments cultured on MS medium containing 1.0㎎/L 2,4-D in cv. group 'Nakhab' and maintained by consecutive subculture on the same medium every 2-3 weeks without loss of embryogenic ability. Upon transfer to MS basal medium, high frequency somatic embryogenesis was achieved easily from embryogenic callus. Regenerated plantlets through organogenesis and somatic embryogenesis were transplanted to pots and gradually acclimatized to greenhouse condition where they subsequently produced fruits.