• 한국식물병리학회지
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• 제3권3호
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• pp.223-235
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• 1987

황색 모자이크 괴저조반, 위축, 기형, colour breaking의 병미을 나타내고 있는 나리류를 한국의 남부지역에서 채집하였다. 전자현미경에 의해 2종의 바이러스 입자가 분리되어, 이들의 기주범위, 혈청반응, 조즙액중의 부활화 한계, 진딧물의 전반성, 감염엽세포에 있어서 바이러스 입자의 소재양식에 의하여 2종의 바이러스를 동정하였다. Broad bean wilt virus(BBWV) : 이 바이러스는 즙액접종에 의해 8과 23종의 검정식물에 감염이 확인되었다. 복숭아혹진딧물에 의해 쉽게 전반되었다. 내열성은 $70\%$, 내희석성은 1,000배, 내보존성은 6일이였다. 바이러스 입자의 형태는 직경 약 28nm의 소구형이다. 혈청시험에서 본 바이러스는 BBWV의 항혈청과 특이적인 반응대를 형성했다. 병엽절편의 전자현미경적관찰에서는 엽육세포의 세포질, 액포, 핵내에서 바이러스 입자의 응집괴 및 결정배열상이 확인되엇고, 특히 세포질내에서는 vesicular body의 발달이 확인되었다. Cncumber mosaic virus(CMV) : 이 바이러스 입자의 형태는 직경 약30nm의 소구형이다. 본 바이러스 CMV-Y의 항혈청과 특이적인 반응대를 형성했다. 감염엽조직의 전자현미경적관찰에서는 엽육세포의 세포질 및 액포내에서 바이러스 입자의 응집괴 도는 결정배열상이 확인되었다. BBWV와 CMV를 건전한 나리류(Lilium tigrium, L. concolor, L. auratum)에 재접종한 결과 BBWV의 접종주에서는 가벼운 병징 또는 colour breaking를 나타내었고, CMV의 접종수에서는 황색 모자이크 또는 괴저조반을 나타냈다.

• 한국식물병리학회지
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• 제14권6호
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• pp.630-635
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• 1998

Tobacco mosaic tobamovirus (TMV), cucumber mosaic cucumovirus (CMV), cucumber green mottle mosaic tobamovirus (CGMMV) and zucchini yellow mosaic potyvirus (ZYMV) from individual fruits and seeds of hot pepper and cucumber were detected by the reverse transcription-polymerase chain reaction (RT-PCR). The dilution end-points for RT-PCR in curde sap from TMV. and CMV - infected hot pepper leaves and CMV - and CGMMV-infected cucumber leaves were 10-5. However, the amount of PCR product obtained from preparation of ZYMV-infected cucumber leaf was 10-fold lower than those of CMV or CGMMV-infected cucumber leaves. In hot pepper, both TMV and CMV were detected in all parts of the fruit wall tissue, but the yields of PCR products in the fruit stalk and its surrounding tissues were higher than those of the end parts of the fruit. On the other hand, in cucumber fruit infected with CMV, CGMMV or ZYMV, the fruit wall tissue and seed located in both stalk and end parts showed higher yields of PCR products than those of intermediate parts. Of five viruses that were analysed, only TMV in hot pepper seed, and CGMMV and CMV in cucumber seed were detected in testa parts.

• 식물조직배양학회지
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• 제24권3호
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• pp.153-160
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• 1997

오이 모자이크바이러스(CMV, cucumber mosaic virus)는 작물의 생산량과 품질에 심각한 피해를 주기 때문에 외피단백질 유전자(CP, coat protein gene)를 도입하여 저항성 작물를 개발하고자 하였다. CMV CP유전자가 도입된 형질전환 담배 39 계통을 대상으로 오이모자이크바이러스 저항성을 검정하였다. 바이러스 저항성은 바이러스 감염으로 인한 생장 억제정도, 병징발현에 따른 잎모양의 변화로서 고도저항성, 저항성, 중간성, 감수성 등으로 판정하였고 39개 계통중 16 계통이 뚜렷한 바이러스 저항성을 보였다. 특히, 저항성 계통중 2 계통은 생장량과 잎모양에서 다른 저항성 계통보다 우수하여 고도저항성으로 세분하였다. 각 형질전환계통에서 CP단백질과 CP RNA 생성량을 조사하였는바, CP단백질 생합성은 대부분의 저항성과 감수성계통에서 검출되어 저항성과 특별한 관련을 인정할 수 없었으나 CP RNA는 대부분의 저항성 및 중간성 계통에서 다량 축적되는 경향을 보여 CP RNA가 저항성에 좀더 밀접함을 알수 있었다. 그러나 고도저항성 계통에서는 CP RNA가 검출되지 않아 저항성의 근원을 파악하기 위해서는 계속적인 연구가 요구된다.

• Molecules and Cells
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• 제19권2호
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• pp.228-231
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• 2005

Recent characterization of several genes involved in plant defense responses suggested that ubiquitin-mediated protein degradation has a role in these responses. We isolated two cDNAs (NtUBA1 and NtUBA2) encoding ubiquitin-activating enzyme (E1) from Nicotiana tabacum cv. BY-2. The open reading frames of both encoded 1080 amino acids, corresponding to molecular masses of 120 kDa. The E1s and corresponding transcripts were upregulated by infection with tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV), and to a lesser extent by cucumber mosaic virus (CMV). Furthermore, they were also upregulated by wounding stress, and the plant hormones salicylic acid, jasmonic acid and the ethylene precursor, aminocyclopropane-1-carboxylic acid (ACC). Our findings support the idea that the ubiquitin-proteasome system plays a role in plant disease defenses.

• The Plant Pathology Journal
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• 제16권2호
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• pp.79-82
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• 2000

No Abstract.See Full-text

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.4-8
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• 2003

Cucumber mosaic virus (CMV) belongs to genus Cucumovirus. The Cucumovirus group contains three distinct members： CMV, Tomato aspermy virus (TAV), and Peanut stunt virus (PSV). The type member, CMV is the most widespread and most studied. CMV is isometric particles about 30 nm in diameter. The genome of CMV is divided into three RNAs. In addition, RNA extracted from virus particles contains a fourth RNA that is a subgenomic RNA generated from RNA3. RNA1 and RNA2 are each encapsidated in separate particles, whereas RNAs3 and 4 are coencapsidated in a third particle. Hence, inoculation by three particles, transmitted either mechanically or by the aphid vector, is required to infect plants.(중략)

• The Plant Pathology Journal
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• 제19권3호
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• pp.166-170
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• 2003

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.137.1-137
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• 2003

A pepper strain of Cucumber mosaic virus (Pf-CMV) induces a mild chlorotic spot symptom in zucchini squash at 9 days post-inoculation (dpi), wile Fny strain of CMV causes severe mosaic and stunting symptom at 4 dpi in this host. Pseudorecombinants were constructed between the two strains, and assessments of symptom severity were indicated that both RNA2 and RNA3 were responsible for both mildness and the slow appearance of symptom elicited by Pf-CMV in zucchini squash. With various RNA2 and RNA3 chimeras between two strains of CMV, the genetic symptom determinants of phenotype of Pf-CMV were mapped to Tyr residue at positions amino acid 267 in 2a protein and at positions amino acid 168 in 3a movement protein (MP). Chimeras changed the sequences (both changed Tyr to lie) in the codons of both amino acid 168 of 3a MP and amino acid 267 of 2a protein were resulted in the high RNA accumulation, severity of symptom, and the rapid systemic spread, suggesting that 2a replicase as well as MP is involved in virus movement. The RNA accumulation pattern of all pseudorecombinants and chimeras are identical in protoplast of zucchini squash, indicating the virus movement is responsible for the phenotypes of two CMV strains rather than virus replication.

• 한국식물병리학회지
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• 제3권4호
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• pp.261-269
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• 1987

포장에서 고추에 황화, 엽맥록대 그리고 엽맥주름을 일으키는 바이러스가 분리되었다. 이 바이러스는 PVY항혈청과 ELISA 검정에서 반응하였으나 CMV, TMV, TBRV, AMV, TSWV, TEV, PMV, TRSV와는 반응하지 않았다. 바이러스 입자의 전자현미경 검경 결과 길이가 750-760nm의 사상형 입자였다. 이 바이러스는 진딧물에 의해 쉽게 전염되었으며 기주범위는 PVY와 비슷하였으나 Chemopodium amaranticolor와 C. quinoa에 전신 감염을 일으켰다.

• The Plant Pathology Journal
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• 제27권4호
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• pp.372-377
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• 2011

A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon biological, serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata, Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. 'Xanthi-nc', Capsicum annuum, Solanum lycopersicum, Solanum melongena, Cucurbita pepo, and Z. mays. In addition, chlorotic local rings on inoculated leaves along with severe mosaic, malformation, and fern leaf symptoms on upper systemic leaves were shown in N. glutinosa plants. Complete nucleotide sequences of each genomic RNA segment was determined and compared to those of the other CMV strains. Comparison of the nucleotide sequence of 1a open reading frame (ORF) revealed approximately 89.2-92.4% sequence identity with each CMV subgroup IA and IB strain, while showing only 78% sequence identity with CMV subgroup II. Nucleotide sequence analysis of RNA2 ORFs revealed 85.3-97.6% sequence identity with subgroup I. In ORFs of RNA3, levels of nucleotide sequence identities were higher than 92-99.2% with CMV subgroup I and lower than 82% with CMV isolates of subgroup II. These results suggest that CMV-ZM isolate is more closely related to subgroup I than subgroup II and therefore, CMV-ZM isolate might be classified into as CMV subgroup I based on biological and molecular analysis.