• KSBB Journal
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• 제13권2호
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• pp.155-161
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• 1998

The hairy roots of Crotalaria sessiliflora were induced from the tissue segments infected with Agrobacterium rhizogenes ATCC 15834. The induced hairy roots were subjected to paper electrophoresis fro the detection of opine-positive clones which were considered to have been transformed. Mannopine and agropine were presented in hairy root clones while mannopine was presented in two hairy root clones. Eight hairy root clones were selected and cultured in MS, B5 and WP media. Each of hairy root clones was showed a difference in branch pattern and growth rate. The best culture medium and culture conditions of hairy roots were in $\frac{1}{2}$MS(3% sucrose, pH 5.7) liquid medium at 25$^\circ C$, 70 rpm under dark, the growth rate in $\frac{1}{2}$MS liquid medium was increased with 210-fold more than that of inoculated hairy roots and with 2-fold more than that in MS liquid medium. Also, the adequate condition for hairy root growth was such that concentration of KH$_2PO$_4 was 1.25mM and the ratio of NH${_4}{^+}$ : NO${_3}{^-}$ was 1 to 3 in MS medium. The presence of pyrrolizidine alkaloids, monocrotaline, in the hairy roots was detected by TLC.

• Archives of Pharmacal Research
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• 제27권5호
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• pp.544-546
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• 2004

Phytochemical investigation of the whole plants of Grata/aria sessiliflora L. led to the isolation of four flavonoids. The structures of these compounds were identified as 2',4',5,7-tetrahydroxy-isoflavone (1), 2',4',7-trihydroxyisoflavone (2), 4',7-dihydroxyflavone (3), and isovitexin (4) using spectroscopic analysis. Among these, compounds 2, and 3 have not been reported from Crotalaria species, whereas compounds 1, and 4 were reported from this plant for the first time.

• 동아시아식생활학회지
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• 제19권2호
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• pp.180-186
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• 2009

활나물 잎을 에탄올과 메틸렌 클로라이드 혼합용매(3:1)로 추출하였다. 이중 가장 활성이 높은 유효성분을 추출 및 분리하기 위해서 당과 지방 성분을 제거하고, water와 n-butanol 용매를 1:1 비율로 혼합하여 분획 추출하였다. n-butanol층 은 감압 농축 후, Sephadex LH-20이 충진된 column chromatography를 이용하여 10개(Fr1, Fr2, Fr3, Fr4, Fr5, Fr6, Fr7, Fr8, Fr9, Fr10)의 소 분획물을 얻었다. 분획물의 각종 항산화 활성 결과 Fr1, Fr4, Fr6에서 높은 항산화 활성을 나타내었다(p<0.05). 분획물중 활성이 강한 F1, F4 및 F6에서 미지의 물질을 발견하였으므로 각종 기기분석을 통해 그물질을 동정 중에 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.684-685
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• 2006