• 대한영양사협회학술지
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• 제25권1호
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• pp.1-16
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• 2019

This study examined the microbiological quality of daycare center meals. Six menu items from five daycare centers in Daegu included uncooked processed foods (seasoned cucumber and lettuce salad), post-preparation after cooking processed foods (rolled omelet and seasoned soybean sprout), and cooking processed foods (panbroiled beef with oyster sauce and seasoned pork roast). Microbiological analyses were performed for the aerobic plate counts (APC), coliforms, and Escherichia coli. The analyses were conducted in July and November 2017. The mean APC and coliform count of seasoned cucumber decreased significantly from 4.71 log colony forming units (CFU)/g and 2.50 log CFU/g in July to 4.07 log CFU/g and 1.78 log CFU/g in November, respectively (P<0.01 and P<0.001). The APC of panbroiled beef with oyster sauce and seasoned pork roast were significantly lower in July (1.84 and 1.79 log CFU/g) than in November (2.41 and 2.28 log CFU/g) (P<0.001). The coliform counts of panbroiled beef with oyster sauce and seasoned pork roast were significantly greater in November (2.11 and 1.62 log CFU/g) (P<0.001). E. coli was not detected. Among the foods prepared using the three preparation processes, the post-preparation after cooking processed foods had the lowest microbial quality. The APC and coliform counts of cooking processed foods were satisfactory in July, with an acceptable rating for pan-broiled beef with oyster sauce in November. Time-temperature control and the prevention of cross-contamination are essential during meal production for food safety, regardless of the season.

• Genomics & Informatics
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• 제19권3호
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• pp.31.1-31.9
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• 2021

Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. For better understanding the difference between Leptospira isolates causing mild and severe leptospirosis, illumina sequencing was used to sequence genomic DNA in both serotypes. DNA of Leptospira isolated from two patients, one with mild and another with severe symptoms, were included in this study. The paired-end reads were removed adapters and trimmed with Q30 score using Trimmomatic. Trimmed reads were constructed to contigs and scaffolds using SPAdes. Cross-contamination of scaffolds was evaluated by ContEst16s. Prokka tool for bacterial annotation was used to annotate sequences from both Leptospira isolates. Predicted amino acid sequences from Prokka were searched in EggNOG and David gene ontology database to characterize gene ontology. In addition, Leptospira from mild and severe patients, that passed the criteria e-value < 10e^-5 from blastP against virulence factor database, were used to analyze with Venn diagram. From this study, we found 13 and 12 genes that were unique in the isolates from mild and severe patients, respectively. The 12 genes in the severe isolate might be virulence factor genes that affect disease severity. However, these genes should be validated in further study.

• 급식외식위생학회지
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• 제3권1호
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• pp.8-18
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• 2022

Dry-aging is one of the traditional aging processes, especially for beef. This aging process is being popular, because it produces unique brown/roasted flavor and texture that consumers prefer. However, as it is exposed to outside without packaging food safety concerns have been raised. The objective of this study was to investigate the presence of total aerobic bacteria (TAB) and pathogenic bacteria in manufacturing environment and suggest the safety management plan for the production of dry-aged meat. Surface samples from 66 environmental and 6 beef carcass samples were collected. According to the monitoring results, the contamination levels of TAB were in the order of shelves (5.4±1.1 Log CFU/cm²), cotton gloves (2.9±0.2 Log CFU/cm²), and door knobs (2.8±0.4 Log CFU/cm²) in the dry-aging room. In the door knobs, the level of mold was higher than that of yeast. These results indicate that the mold spores may be cross-contaminated with environmental factors inside the aging room. The risk factors that may occur during the manufacturing process were presented and possibility of risk was determined. From the aspect of microbiology, aging and trimming steps were determined as the critical control points. The temperature of the aging room should be maintained below 10℃ and the humidity below 75-85%. Based on the monitoring and the risk assessment of the dry-aging process, we prepared the safety management plan for the production of dry-aged meat, and it should be useful in improving the food safety of dry-aged meat.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제29권1호
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• pp.43-51
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• 2023

Purpose: To accommodate the increasing number of patients during the COVID-19 pandemic, numerous portable HEPA filter units (PHUs) were installed in the general wards of existing medical facilities(EMFs) to convert them into emergency conversion facilities (ECFs). The purpose of this study was to build a dataset in preparation for emerging infectious diseases in the post-COVID-19 era by analyzing the construction and operation of ECFs. Methods: Field investigations were conducted during ECF operation periods based on the analysis of heating, ventilation, and air conditioning (HAVC) system design documents for six ECFs across Korea. Interviews were conducted with facility managers during the field investigations. Results: When constructing an ECF within an EMF, the installation status and characteristics of the existing system should be considered. Field investigations and verifications of the operation of HAVC systems must be conducted beforehand for smooth ECF operations. If heating and cooling are required with indoor air circulation type equipment in an ECF zone, the implementation of a heating and cooling method that can satisfy the comfort requirements of the occupants while minimizing cross-contamination is essential. When using PHUs that do not meet the performance standards required by medical equipment, the noise level resulting from such equipment operation must be evaluated and improved. Implications: For EMFs, various guidelines that can be referred to for the construction and operation of ECFs must be developed to prepare for emerging infectious diseases in the future.

• Journal of Veterinary Science
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• 제23권1호
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• pp.9.1-9.11
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• 2022

Background: Escherichia coli, which causes subclinical or clinical mastitis in cattle, is responsible for transmitting antimicrobial resistance via human consumption of raw milk or raw milk products. Objectives: The objective of this study was to investigate the molecular characteristics of 183 E. coli from bulk tank milk of five different dairy factories in Korea. Methods: The molecular characteristics of E. coli such as serogroup, virulence, antimicrobial resistance, and integron genes were detected using polymerase chain reaction and antimicrobial susceptibility were tested using the disk diffusion test. Results: In the distribution of phylogenetic groups, group D was the most prevalent (59.6%) and followed by group B1 (25.1%). The most predominant serogroup was O173 (15.3%), and a total of 46 different serotypes were detected. The virulence gene found most often was fimH (73.2%), and stx1, fimH, incC, fyuA, and iutA genes were significantly higher in isolates of phylogenetic group B1 compared to phylogenetic groups A, B2, and D (p < 0.05). Among 64 E. coli isolates that showed resistance to at least one antimicrobial, the highest resistance rate was observed for tetracyclines (37.5%). All 18 integron-positive E. coli carried the integron class I (int1) gene, and three different gene cassette arrangements, dfrA12+aadA2 (2 isolates), aac(6')-Ib3+aac(6')-Ib-cr+aadA4 (2 isolates), and dfrA17+aadA5 (1 isolate) were detected. Conclusions: These data suggest that the E. coli from bulk tank milk can be an indicator for dissemination of antimicrobial resistance and virulence factors via cross-contamination.

• 근관절건강학회지
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• 제31권1호
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• pp.42-52
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• 2024

Purpose: This study aimed to identify the factors affecting compliance with personal protective equipment (PPE) use among medical device reprocessing staff. Methods: This descriptive cross-sectional study included 163 cleaning staff members from ten general hospitals in Seoul and Gyeonggi. Data were collected using self-report questionnaires administered between July and September 2023. Analysis included t-tests, ANOVA, Pearson's correlation coefficient, Bonferroni correction, and multiple regression, conducted using SAS ver.9.4. Results: Statistically significant differences in compliance with PPE were found based on department and exposure to contamination within six months (t=-2.82, p=.007). Attitudes toward PPE (r=.22, p=.006) and awareness of the safety climate (r=.22, p=.006) showed a statistically significant positive correlation with PPE compliance. Factors influencing use of personal protective equipment by cleaning staff during medical device reprocessing were department, compliance with PPE, and awareness of the safety climate. The explanatory power of these factors was 58.0%. Conclusion: Improving PPE compliance and creating a safe cleaning environment entails fostering a supportive safety climate. Additionally, regular training that takes into consideration the characteristics of the cleaning staff, alongside continuous monitoring, is required.

• Journal of Preventive Medicine and Public Health
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• 제57권1호
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• pp.73-82
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• 2024

Objectives: The unsafe use of pesticides in agriculture represents a major hazard to human health. This study was conducted to investigate the association between pesticide exposure and health symptoms among chili farmers in northeast Thailand. Methods: This cross-sectional analytic study included 141 chili farmers in Sakon Nakhon Province, in northeast Thailand. Data regarding health symptoms were gathered using a self-report questionnaire. A medical technician tested blood cholinesterase activity using an erythrocyte method, and an occupational medicine specialist at Ramathibodi Hospital in Bangkok, Thailand reviewed the results. Associations between personal characteristics, pesticide exposure factors, and health symptoms were analyzed using multiple logistic regression. Results: Of the 141 chili farmers studied, 66.7% experienced pesticide poisoning, as indicated by below-normal cholinesterase levels. Fatigue was the most frequently reported symptom associated with pesticide exposure, affecting 37.6% of participants. This was followed by nausea and vomiting (31.9%), dizziness (14.9%), and dry throat (14.9%). Multivariate logistic regression analysis revealed that several factors were significantly associated with adverse symptoms: amount of work experience, volume of pesticides used, use of chemical pesticides, use of leaking containers during spraying, direct pesticide exposure while working, contact with pesticide-soaked clothing, consumption of food and drinks in the fields, and blood cholinesterase level indicating risk. Conclusions: This study suggests potential health risks for chili farmers stemming from exposure to and contamination by pesticides used in agricultural practices. To mitigate these risks, it is essential to supply personal protective equipment and to implement educational programs aimed at improving protective behaviors among farmers.

• 복식
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• 제51권2호
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• pp.149-167
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• 2001

Today, every culture has taken on the compromise form by means of the cultural difference, variety, and pluralism according to the internationalization and the advance so that it has been developed toward the half-blooded and multilayered the aspect. In accordance with this current of the times, this thesis observed the feminism found in the multiculturism of the end of the 20th century, the third world, and technology with the side of the tendency of modern fashion, considered the hybride phenomenon which is pulling down the wall between culture and genre due to the social diversification. and predicted the fashion trend do 21st century serf on it. Multiculturism is the movement that began to arise in the academic world of America and the literary world form the close of 1980's in accepting the variety of culture and regarding the culture with the more balanced and wide view and just as it is, it means the attitude of accepting one or more cultures of variely and the position of taking interest in the culture of minority race not the culture of a governing race. It is the fashion of feminism adapts dualism like unisex, androgynous look, etc of bisexual lendency in the 1980's, it shows new style with crossover of liberal sense because there is not the difference of sex in fashion. The eco-feminism pursues the natural sexuality not being instrumental and dismantling and expressed it in the Gender expression of an integrated human being. The trend of ethnic fashion in the close of 20th century is that the element of hippie is working so strongly. By adding embroidery of Oriental style, accessories of Indian style, feathers, beads, a hempen hood to the ethnic costumes of Asia and Latin, is shows the figure of ethnic hippie. As the cycler fashion is the future clothes through technology of computer, it uses a cool glass material bringing up the image of a spacesuit in order to expresses cyber image through artificial color combination of sheen colors, Though this techno-color fashion has established the fresh stimulation and the innovative aspect with ultramodern materials and image of futurism, it transmits a hope of estranged people and the natural elements. Hybride means a cross and mixture of animal and plant in Korean and is also called fusion. The phenomenon of hybrid predicts to comes the period of a cross and variation because something completely new comes into the world by contamination, mixture and compromise through meeting something different each other and it has on advantage of developing something existing to one more stage. It is prospected that in the society of 21st century, the borderline of traditional gender will be disappeared, variety and individuality will determine the individual behavior, and the masculine value will be substituted by feminine value. In the society giving priority to feminine value, a fashion stuck closely to women is what must reflect lives of woman under the proposition of woman's beauty, being on original function. So, it is considered that a fashion with added convenience and practicality having the function which is easy to put on, comfortable to act, able to express solves so much, and able to show various appearances according to T.P.O will get into the spotlight.

• 한국식품영양과학회지
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• 제45권10호
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• pp.1497-1507
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• 2016

본 연구는 서울 경기지역에 소재한 외식업체의 종사자를 대상으로 2014년 5월~2014년 7월까지 설문조사를 시행하였다. 설문은 총 500부가 회수되었고, 이 가운데 통계분석이 가능하고 유효한 458부를 분석 자료로 이용하였으며, 외식업체 경영자와 종업원의 HACCP(Hazard Analysis and Critical Control Point) 선행요건에 대한 중요도와 수행도를 IPA(Importance-Performance Analysis) 기법을 이용하여 비교 분석함으로써 향후 소규모 외식업체 HACCP 적용을 위해 선행되어야 할 위생관리 항목 중 우선적으로 교육해야 할 항목에 대한 연구 자료를 제시하고자 하였다. 외식업체 종사자를 대상으로 HACCP 선행요건 15개 항목의 중요도와 수행도를 분석한 결과, 전체 항목 중 중요도에 비해 현장 수행도의 점수가 유의적으로 낮게 조사된 대표적인 항목은 '종업원의 손 위생 및 청결한 위생복장의 착용', '포획된 해충 설치류를 정기적으로 확인', '입고되는 원재료의 확인 및 검사', '식품안전사항 개선조치 후 결과 기록 유지', '모니터링 도구의 정기적 세척 소독'으로 나타났다. 이에 HACCP 선행요건 기준에 따라 위의 5개 항목은 중요도-수행도의 점수 차이가 매우 크게 나타났으므로 위의 5개 항목내용을 중심으로 현장 수행력을 높일 수 있는 교육내용으로 구성해야할 것으로 사료되었다. HACCP 선행요건에 대한 세부항목별 IPA 분석 결과, 수행도가 상대적으로 낮아 집중하여 개선해야 할 영역인 2사분면의 경우 소규모 업체는 '포획된 해충 설치류를 정기적으로 확인', '종업원의 손 위생 및 청결한 위생복장 착용', '음식물 교차오염 방지' 항목이 조사되었고, 중규모 업체는 '조리장의 해충 설치류 유입 차단', '식품안전사항 개선조치 후 결과 기록 유지' 등으로 나타났다. 2사분면 항목은 종사자들이 매우 중요하게 인식하고 있지만, 수행도가 낮아 외식업체 규모에 따른 적합한 개선이 필요할 것으로 사료되었다. 이상의 연구를 종합한 결과, 중 소규모 전체 외식업체를 대상으로 중요도는 높지만, 수행도가 상대적으로 낮아 집중하여 개선해야 할 위생관리항목은 '포획된 해충 설치류를 정기적으로 확인', '종업원의 손 위생 및 청결한 위생복장 착용', '음식물 교차오염 방지' 등으로 나타나 이 항목들을 중심으로 지속적인 모니터링과 개선활동을 전개할 필요가 있겠다. 또한, 소규모 업체를 대상으로 집중하여 개선해야 할 항목은 '포획된 해충 설치류를 정기적으로 확인', '종업원의 손 위생 및 청결한 위생복장착용', '음식물 교차오염 방지' 등이었고, 중규모 업체만 나타난 항목은 '조리장의 해충 설치류 유입 차단', '식품안전사항 개선조치 후 결과 기록 유지' 등으로 나타났다. 이에 이 항목들에 대하여 실제 성공적으로 관리되는 외식업체 실제 사례를 통한 위생교육이 강화될 필요가 있으며, 위생교육에 따른 중 소규모 외식업체 현장에서 가장 개선되어야 할 항목들에 대하여 보완된 위생관리점검표를 메뉴얼화하여 철저하게 관리 감독해야 하겠다.

• 미생물학회지
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• 제41권3호
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• pp.216-224
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• 2005

혈장분획제제 중 혈액응공인자제제와 일부 면역글로불린제제는 혈장에 존재하는 다양한 단백질로부터 유효한 단백성분만을 선택적으로 분리 정제하기 위해 크로마토그래피 방법을 사용하여 생산된다. 효율적인 세척(cleaning) 공정이 이루어지지 않는다면 크로마토그래피는 다양한 종류의 불순물뿐만 아니라 혈액 중 내재 또는 오염 가능성이 있는 위해인자가 오염될 가능성이 있다. 본 연구에서는 혈장분획제제 제조공정에 사용되는 크로마토그래피의 세척 공정에서 혈장유래 바이러스의 제거 및 불활화 공정의 검토 강화로 혈장분획제제의 안전성을 확보하기 위해 크로마토그래피 세척 검증 시스템을 구축하고자 하였다. 크로마토그래피 세척 공정 중 바이러스 제거 검증을 위해 혈장유래 바이러스 중 물리${\cdot}$화학적 처리에 가장 큰 저항성을 갖는 human parvovirus B19의 모델 바이러스의 porcine parvovirus(PPV)를 대상으로 real-time PCR 정량법을 확립하였다. PPV에 특이적인 primer를 선별하였으며 형광염료 SYBR Green I을 사용하여 PPV DNA를 정량하였다. 세포배양법에 의한 감염 역가와 비교한 결과 PCR 민감도는 1.5 $TCID_{50}/ml$이었다. 확립된 검증법의 신뢰성(reliability)을 보증하기 위해 실험법의 특이성(specificity), 재현성(reproducibility) 등을 검증하였다. 구축된 검증시스템을 thrombin 분리${\cdot}$정제를 위한 SP-Sepharose 양이온 크로마토그래피 공정과 factor VIII 분리${\cdot}$정제를 위한 Q-Sepharose 음이온 크로마토그래피 공정에 적용하여 크로마토그래피 세척 검증을 실시하고, 세척 검증 시스템의 적합성을 확인하였다.