• 제목/요약/키워드: Cross-Contamination

검색결과 263건 처리시간 0.024초

Biosafety Challenges for the Microbiology Laboratory

  • Montville, Thomas J.
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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    • pp.66-70
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    • 2005
  • Microbiology research must be conducted in a fashion that assures the health and well being of the researcher and the safety of the community. This lecture raises awareness of biosafety issues and discusses how the interaction of the pathogen being studied, the person conducting the research, and the practices being used can be manipulated to assure safety. The characterization of pathogens into Risk Groups, how these relate to Biosafety Levels, and the personal practices and laboratory design criteria associated with each Biosafety Level are explained. The importance of preventing or containing aerosols, limiting opportunities for cross-contamination, and taking a flexible multi-component approach to biosafety are emphasized.

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The Analysis of VOCs by GC/MS with Whole Column Coldtrapping on a Fused Silica Capillary Column in Indoor Environment

  • Dai, Shugui;Zhang, Lin;Bai, Zhipeng
    • 분석과학
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    • 제8권4호
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    • pp.829-834
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    • 1995
  • Whole column coldtrapping(WCC) on a fused silica capillary (FSCC)combined with GC/MS analysis was evaluated for use in the investigation of volatile organic compounds(VOCs) in indoor air. Research had indicated that a temperature of $-80^{\circ}C$ is optimal for WCC. Samples were analyzed on a $50m{\times}0.2mm$ cross-linked 5% phenylmethylsilicone fused silica column. Liquid nitrogen was used as the coolant for the peak resolution significantly. The analysis can be performed quickly and conveniently. More than 112 of VOCs were determined in the samples from three typical indoor environment including: (1) a room which had just been decorated involving building materials and paints; (2)a kitchen used for Chinese cooking, and (3) a room had tobacco smoke. The method is could be readily applied to rapid sample screening for VOCs contamination surveys or initial investigations with its valid and simple sampling and analytical technique.

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잔류 Sulfamethazine 검출용 ELISA 개발에 관한 실험적 연구 (Experimental Study on Development of ELISA Method for the Detection of Sulfamethazine Residues)

  • 임윤규;김성희
    • 한국식품위생안전성학회지
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    • 제10권4호
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    • pp.213-217
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    • 1995
  • A screening method has been developed for detecting sulfamethazine(SMZ) contamination of meat or feeds by using horseradish peroxidase (HRP) labeled protein A (Prot AHRP)and an indirect competitve enzyme-linked immunosorbent assay(ELISA). The assay is based on competitve binding of guinea pig anti-SMZ with SMZ in smaple and SMZ-gelatin conjugate(SMZ.GEL). Percent binding (B.Bo$\times$100) was calculated from the absorbance in the absence (B0) and presence (B) of SMZ. By the sandard curve prepared by plotting log(SMZ) vs percent binding of each known reference solution, the detection limit was 1.0ppb or less. Cross reacton with sulfadimethoxine, sulfaguaniding, sulfamerazine, sulfamthoxpyridazine, sulfanilamide, sulfisomidine and sufisoxazole were not observed. But sulfamerazine crossreacted in the test. The EC-50 value (concentration causing 50% inhibition of color development compared with blank) of sulfamerazine was 2.0 ppm. Further quality control will make the ELISA system ideal for the detection of SMZ in meat or feeds.

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Effectiveness of Antimicrobial Starch Coating Containing Thyme Oil against Salmonella, Listeria, Campylobacter, and Pseudomonas on Chicken Breast Meat

  • Goswami, Namita;Han, Jung-Hoon;Holley, Richard A.
    • Food Science and Biotechnology
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    • 제18권2호
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    • pp.425-431
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    • 2009
  • Antimicrobial coating on chicken carcasses may reduce the effects of cross-contamination and improve product shelf-life and safety. Thyme oil was mixed at 0.5%(v/v) with a pre-gelatinized pea starch coating solution. The coating solution was spread on chicken breast meat after inoculation with selected spoilage and pathogenic bacteria. After inoculation, the chicken meats were packaged in plastic bags and stored at $4^{\circ}C$. During 12 day storage, total aerobic bacteria, lactic acid bacteria, and inoculated organisms were counted at 4 day intervals. Thyme oil treatments reduced the viability of Salmonella as well as the growth of Listeria and Pseudomonas by 2 log CFU/g, and appeared to eliminate inoculated Campylobacter during storage. The addition of thyme oil increased the viscosity of the pre-gelatinized pea starch solution. The results suggested that thyme oil inclusion in an edible starch coating may be a satisfactory delivery system to enhance the safety of processed fresh meat.

Low Resolution Near-Infrared Stellar Spectra Observed by CIBER

  • Kim, MinGyu;Lee, Hyung Mok
    • 천문학회보
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    • 제41권1호
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    • pp.76.2-76.2
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    • 2016
  • We present near-infrared (0.8 - 1.8 microns) spectra of 63 bright (J_mag < 10) stars observed with Low Resolution Spectrometer (LRS) onboard the rocket-borne Cosmic Infrared Background Experiment (CIBER). Two Micron All Sky Survey (2MASS) photometry information is used to find cross-matched stars after reduction and extraction of the spectra. We identify the spectral types of observed stars by comparing with spectral templates from the Infrared Telescope Facility (IRTF) library. All the observed spectra are consistent with late F to M stellar spectral types, and we identify various infrared absorption lines. As our observations are performed above the Earth's atmosphere, our spectra are free from telluric contamination. Including HST/NICMOS and Cassini/VIMS, the spectral coverage has rarely been achieved in space, and the methods developed here can inform statistical studies with future low-resolution spectral measurements such as GAIA photometric and radial velocity spectrometer.

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자화된 플라즈마 내에서의 단분산 입자의 하전량 특정 (Measurement of Monodisperse Particle Charging in Unmagnetized and Magnetized Plasmas)

  • 한장식;안강호;김곤호
    • 반도체디스플레이기술학회지
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    • 제1권1호
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    • pp.35-40
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    • 2002
  • Understanding of charging properties of a small particle is necessary to control the particle contamination and to improve productivity of the electronic device in the plasma aided semiconductor manufacturing processes. In this study, the effects of both magnetic field and particle size on the charging properties are experimentally investigated in collisional dusty plasmas. The experiments carried out in the system consisted of a monodisperse particle generation system, a DC magnetized plasma generation system and a charge measurement system. The plasma chamber is made of cross-shape Pyrex surrounded by magnetic bucket (composed of 12 permanent magnetic bar) to confine the plasma. DC magnetic field up to 250G are applied to the plasma zone by external magnetic coil. Previous work shows the charging effect clearly increase with increasing the size of the particle and plasma density, as it was expected.

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제2주제: 외국의 학교급식의 관리 현황 (Food Safety Policies on School Lunch Program in Foreign Countries)

  • 노병의
    • 한국식품위생안전성학회:학술대회논문집
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    • 한국식품위생안전성학회 1997년도 추계학술세미나 - 위생적인 학교급식의 관리방안
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    • pp.13-19
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    • 1997
  • Foodborne diseases are serious problems throughout the world, because new pathogens have emerged. Nowadays many students eat school lunch provided by school lunch provided by school lunch program. They are threatened by pathogens transmitted by foods. many school children became victims of foodborne diseases. Most foodborne outbreaks were caused by mishandling foods at food service establishments. Food safety practices of school lunch programs in foreign countries were reviewed. Temperature control is the most critical part of keeping the foods safe. In order to prevent foodbrorne diseases in schools, the managers in charge of school lunch program and officials in charge of food safety of local health department try to make sure that foods are out of danger zone of temperature. Prevention of cross contamination, and sanitization of raw foods and utensils are also emphasized by school lunch program managers and health officials.

라벨 스무딩을 활용한 치은염 이진 분류기 캘리브레이션 (Calibration for Gingivitis Binary Classifier via Epoch-wise Decaying Label-Smoothing)

  • 이상현
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2021년도 추계학술대회
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    • pp.594-596
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    • 2021
  • Future healthcare systems will heavily rely on ill-labeled data due to scarcity of the experts who are trained enough to label the data. Considering the contamination of the dataset, it is not desirable to make the neural network being overconfident to the dataset, but rather giving them some margins for the prediction is preferable. In this paper, we propose a novel epoch-wise decaying label-smoothing function to alleviate the model over-confidency, and it outperforms the neural network trained with conventional cross entropy by 6.0%.

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식품의약품안전처의 위생점검표에 의한 육아지원기관 급식소 평가 및 개선대책 (Assessment and Corrective Measures of Child-care Foodservices by Sanitary Inspection Checklist Suggested by Korea Food and Drug Administration)

  • 안수정;문혜경
    • 대한영양사협회학술지
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    • 제21권3호
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    • pp.227-240
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    • 2015
  • The purpose of this study was to assess the hygiene status of 145 child-care foodservices, which were newly registered in the Center for the Children's Foodservice Management (CCFSM) in Changwon, Gyeongnam. Sanitary inspection checklist (40 food safety items) of Korea Food and Drug Administration (KFDA) was used by the CCFSM in Changwon, Gyeongnam. Average score from the total safety items in 145 child-care foodservices was 21.41 points out of 40 points. Score gaps between the lower group (71 foodservices, 13.92 points) and higher group (74 foodservices, 28.61 points) showed a significant difference (P<0.001). The top five priorities of sanitary measures needing corrective actions for child-care foodservices were as follows: 'provide hand washing and sanitizing tools, and supply manuals on how to wash hands properly', 'supply record form for access/inspection', 'conduct education for cooks on standards in the selection of sanitizer for raw vegetables and proper methods to wash and sanitize raw vegetables', 'provide a refrigerator and a freezer with installed thermometers and temperature record monitoring logs', 'encourage separation of contamination operating zone and clean zone or conduct training for cooks on ways to prevent cross-contamination by performing work separately by the hour'. For the higher group, most were national/public facilities (83.3%), whereas in the lower group, private facilities (62.1%) were more common than national/public ones (37.9%). Therefore, a significant difference (P<0.001) was detected in the two groups. These private facilities should be supported.

넙치 양식장 HACCP 적용을 위한 위생관리에 대한 연구 (Research on Sanitation Control for an HACCP Application for a Flatfish (Paralichthys olivaceus) Aquaculture Farm)

  • 지보영;민진기;김태진;최재석;박선미
    • 수산해양교육연구
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    • 제25권5호
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    • pp.1179-1191
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    • 2013
  • Many sanitation control problems due to aging facilities and equipment were identified when applying an HACCP system to a flatfish (Paralichthys olivaceus) aquaculture farm. Specifically, the major problems included a lack of awareness about worker hygiene, lack of management of the use of fish medicines, and vulnerability to secondary contamination by cross-contamination owing to a failure to separate breeding tools used for healthy and unhealthy fish. Therefore, the management standards on the farm regarding the surrounding environment, facilities and equipment, breeding tools, feed and medication, and the hygiene of practitioners must be improved. The hygiene management standards were divided into different procedures such as the management of farm hygiene and environmental sanitation, facilities and equipment, fish hygiene, feed, medications, water, and the stocking and shipping of fish. For each procedure, we established the management standards, inspection period, inspection procedures, and how to deal with errors that occur, to enable hygiene management by a small number of managers. Additionally, an inspection system and record form to implement an HACCP system were developed to maintain systematic management. The management and inspection of all aspects of the farm were designed to be easily managed by the supervisor.