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Regulation of Gene Expression for Amino Acid Biosynthesis in the Yeast, Sacchromyces cerevisiae

  • Lea, Ho Zoo
    • Proceedings of the Zoological Society Korea Conference
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    • 1995.10b
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    • pp.82-82
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    • 1995
  • Regulation of enzyme synthesis by transcriptional and translational control systems provides rather stable adaptation to change of amino acid level in the growth medium, while manipulation of enzyme activity through endproduct feedback inhibition represents rather short-term and reversible ways of adjusting metabolic fluctuation of amino acid level. Various control mechanisms interplay to regulate genes encoding enzymes for amino acid biosynthesis in the yeast, Sacchromyces cerevisiae. When amino acids are in short supply, genes under a cross-pathway regulatory mechanism Or general amino acid control (general control) increase their action, in which Gcn4p is the major positive regulator of gene expression. When cells are cultured in minimal medium, basal level expression is also regulated by supplementary control elements, where inorganic phosphate level is additionally involved. Most of amino acid biosynthetic genes are also regulated by the level of endproduct of the pathway. This pathway-specific regulatory mechanism is called specific amino acid control (specific controD, under which gene expression is reduced when endproduct is present in the medium. Derepression of a gene through general control can be usually overridden by repression through specific control, where the endproduct level of that particular pathway is high and not limiting. In this presentation, regulatory factors for basal level expression and general control of yeast amino acid biosynthesis will be discussed, m addition to pathway-specific repression patterns and interaction between CrOSS- and specific-control mechanisms. Preliminary results are also presented from the investigation of the cloned genes in the threonine biosynthetic pathway of the yeast. yeast.

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A Study on Accuracy Detection Method for Signal Peak Voltage (신호용 PEAK 전압 정밀검출에 관한 연구)

  • Park, Ho-Chul;Sung, Hyung-Su;Han, Seung-Moon;Han, Jeong-Hoon
    • Proceedings of the KIEE Conference
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    • 2000.07d
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    • pp.2528-2530
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    • 2000
  • In general, Diode makes a major role in electronic circuit. For example, switching of rectifier, cross current of switching rectifier, energy transfer of electronic element and reverse charge of capacitor, voltage insulation, energy feedback from load to power supply, and such as recovery of storaged energy. Generally, We regard power diode as ideal element, but it has a certain boundary actually, specially, We use diode for detecting circuit peak hold voltage signal. It has cut in voltage. It occurs error of measurement value namely. This error, below in region diode voltage drop (0.7v) measurement value is wholesome signal, Specially, We can not get precision data. Therefore, precision level is low between theoretical and measurement data because of error in actual circuit. Conclusionally, In this paper, We define the error concerning to the power diode characteristics which is used detecting of the minute signal, and recommend the method that minimize measurement error.

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The Comparison of Muscle Contraction Time and Modulation Ratio during Reaching Tasks in Hemiparalysis, Elderly and Young Adults

  • Sun, Jeon;Park, Dae-Sung
    • Physical Therapy Rehabilitation Science
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    • v.11 no.2
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    • pp.145-153
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    • 2022
  • Objective: The purpose of this study was to evaluate the Electromyography (EMG) of the upper limb during reaching tasks according to two heights in the sitting position. Design: Cross sectional design Methods: Fifteen hemiplegia, fifteen elderly, and fifteen healthy subjects have participated in this study. The targets (90% length of the subject's arm) were located at the two heights (the eye and xiphoid process). We have recorded EMG signals of seven upper limb muscles (anterior deltoid (AD), posterior deltoid (PD), pectoralis major (Pec), infraspinatus (Inf), supraspinatus (Sup), biceps brachii (Bi), triceps brachii (Tri)). The dependent variables were movement time(s), modulation ratio, working ratio, and the co-contraction ratio of the hemiplegia, elderly, and healthy at the reaching task. Two-way repeated-measures ANOVA (2-heights) was analyzed with the LSD post hoc test. Results: The study results were as follows: (1) The movement time to the target during reaching movement was significantly longer for the hemiplegia and elderly groups compared to the healthy group. (2) The modulation rate was significantly higher at eye height than the xiphoid height in AD, PD, Pec, Inf, Bi muscles, and the hemiplegia group and elderly group were significantly lower than the healthy group. Additionally, the modulation ratio showed a significant interaction between heights and groups. Conclusions: It is expected that the variables using the muscle contraction characteristics, the evaluation method of this study, can be used as an electromyography-based feedback method that can be objectively evaluated and quantified in clinical practice.

Linking nuclear energy, human development and carbon emission in BRICS region: Do external debt and financial globalization protect the environment?

  • Sadiq, Muhammad;Shinwari, Riazullah;Usman, Muhammad;Ozturk, Ilhan;Maghyereh, Aktham Issa
    • Nuclear Engineering and Technology
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    • v.54 no.9
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    • pp.3299-3309
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    • 2022
  • Nuclear energy has the potential to play an influential role in energy transition efforts than is now anticipated by many countries. Realizing sustainable human development and reducing global climate crises will become more difficult without significantly increasing nuclear power. This paper aims to probe the role of nuclear energy, external debt, and financial globalization in sustaining human development and environmental conditions simultaneously in BRICS (Brazil, Russia, India, China, and South Africa) countries. This study applied a battery of second-generation estimation approaches over the period from 1990 to 2019. These methods are useful and robust to cross-countries dependencies, slope heterogeneity, parameters endogeneity, and serial correlation that are ignored in conventional approaches to generate more comprehensive and reliable estimates. The empirical findings indicate that nuclear energy and financial globalization contribute to human development, whereas external debt inhibits it. Similarly, financial globalization accelerates ecological deterioration, but nuclear energy and external debt promote environmental sustainability. Moreover, the study reveals bidirectional feedback causalities between human development, carbon emissions and nuclear energy consumption. The study offers useful policy guidance on accomplishing sustainable and inclusive development in BRICS countries.

Verification of OpenMC for fast reactor physics analysis with China experimental fast reactor start-up tests

  • Guo, Hui;Huo, Xingkai;Feng, Kuaiyuan;Gu, Hanyang
    • Nuclear Engineering and Technology
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    • v.54 no.10
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    • pp.3897-3908
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    • 2022
  • High-fidelity nuclear data libraries and neutronics simulation tools are essential for the development of fast reactors. The IAEA coordinated research project on "Neutronics Benchmark of CEFR Start-Up Tests" offers valuable data for the qualification of nuclear data libraries and neutronics codes. This paper focuses on the verification and validation of the CEFR start-up modelling using OpenMC Monte-Carlo code against the experimental measurements. The OpenMC simulation results agree well with the measurements in criticality, control rod worth, sodium void reactivity, temperature reactivity, subassembly swap reactivity, and reaction distribution. In feedback coefficient evaluations, an additional state method shows high consistency with lower uncertainty. Among 122 relative errors in the benchmark of the distribution of nuclear reaction, 104 errors are less than 10% and 84 errors are less than 5%. The results demonstrate the high reliability of OpenMC for its application in fast reactor simulations. In the companion paper, the influence of cross-section libraries is investigated using neutronics modelling in this paper.

Variation of the Muscle Activity of Erector Spinalis and Multifidus According to Their Respective Cueing When Performing Tasks, Including Tactile Stimulation in Prone Position

  • Gam, Byeong-Uk;Song, Changho
    • Physical Therapy Rehabilitation Science
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    • v.11 no.1
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    • pp.88-96
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    • 2022
  • Objective: Purpose of this study was to compare muscle activity ratio of multifidus to erector spinalis according to various cueing including tactile stimulation to provide an effective strategy to provide verbal and tactile feedback during exercise to provoke multifidus muscle activation. Design: Cross-sectional study. Methods: Participants of this study included 28 healthy adults. Muscle activities of the multifidus and erector spinalis were measured while the participants performed tasks according to the three different methods of verbal cueing and three different tactile stimulation. Surface EMG was used to measure the muscular activity of the muscles during all the tasks. Results: Tactile stimulation to abdomen and lumbar vertebrae showed no significant difference in the muscle activity ratio (p>0.05). However, muscle activity ratio of the multifidus in relation to the erector spinalis was increased when subjects were given verbal instructions to make lumbar curvature with little force and to make lumbar curvature while pulling navel (p<0.05). However, it was decreased when they were provided with verbal instruction to make lumbar curvature with strong force (p<0.05). Conclusions: According to the results, proper verbal instruction was an effective tool to increase the muscular activity of multifidus. This study aimed to find and provide the most appropriate verbal cueing while doing exercises to activate multifidus.

System Identification of Nonlinear System using Local Time Delayed Recurrent Neural Network (지역시간지연 순환형 신경회로망을 이용한 비선형 시스템 규명)

  • Chong, K.T.;Hong, D.P.
    • Journal of the Korean Society for Precision Engineering
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    • v.12 no.6
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    • pp.120-127
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    • 1995
  • A nonlinear empirical state-space model of the Artificial Neural Network(ANN) has been developed. The nonlinear model structure incorporates characteristic, so as to enable identification of the transient response, as well as the steady-state response of a dynamic system. A hybrid feedfoward/feedback neural network, namely a Local Time Delayed Recurrent Multi-layer Perception(RMLP), is the model structure developed in this paper. RMLP is used to identify nonlinear dynamic system in an input/output sense. The feedfoward protion of the network architecture provides with the well-known curve fitting factor, while local recurrent and cross-talk connections provides the dynamics of the system. A dynamic learning algorithm is used to train the proposed network in a supervised manner. The derived dynamic learning algorithm exhibit a computationally desirable characteristic; both network sweep involved in the algorithm are performed forward, enhancing its parallel implementation. RMLP state-space and its associate learning algorithm is demonstrated through a simple examples. The simulation results are very encouraging.

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Wideband Resistive LNA based on Noise-Cancellation Technique Achieving Minimum NF of 1.6 dB for 40MHz (40MHz에서 1.6 dB 최소잡음지수를 얻는 잡음소거 기술에 근거한 광대역 저항성 LNA)

  • Choi Goangseog
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.20 no.2
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    • pp.63-74
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    • 2024
  • This Paper presents a resistive wideband fully differential low-noise amplifier (LNA) designed using a noise-cancellation technique for TV tuner applications. The front-end of the LNA employs a cascode common-gate (CG) configuration, and cross-coupled local feedback is employed between the CG and common-source (CS) stages. The moderate gain at the source of the cascode transistor in the CS stage is utilized to boost the transconductance of the cascode CG stage. This produces higher gain and lower noise figure (NF) than a conventional LNA with inductor. The NF can be further optimized by adjusting the local open-loop gain, thereby distributing the power consumption among the transistors and resistors. Finally, an optimized DC gain is obtained by designing the output resistive network. The proposed LNA, designed in SK Hynix 180 nm CMOS, exhibits improved linearity with a voltage gain of 10.7 dB, and minimum NF of 1.6-1.9 dB over a signal bandwidth of 40 MHz to 1 GHz.

A novel aerodynamic vibration and fuzzy numerical analysis

  • Timothy Chen;Yahui Meng;Ruei-Yuan Wang;ZY Chen
    • Wind and Structures
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    • v.38 no.3
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    • pp.161-170
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    • 2024
  • In recent years, there have been an increasing number of experimental studies showing the need to include robustness criteria in the design process to develop complex active control designs for practical implementation. The paper investigates the crosswind aerodynamic parameters after the blocking phase of a two-dimensional square cross-section structure by measuring the response in wind tunnel tests under light wind flow conditions. To improve the accuracy of the results, the interpolation of the experimental curves in the time domain and the analytical responses were numerically optimized to finalize the results. Due to this combined effect, the three aerodynamic parameters decrease with increasing wind speed and asymptotically affect the upper branch constants. This means that the aerodynamic parameters along the density distribution are minimal. Taylor series are utilized to describe the fuzzy nonlinear plant and derive the stability analysis using polynomial function for analyzing the aerodynamic parameters and numerical simulations. Due to it will yield intricate terms to ensure stability criterion, therefore we aim to avoid kinds issues by proposing a polynomial homogeneous framework and utilizing Euler's functions for homogeneous systems. Finally, we solve the problem of stabilization under the consideration by SOS (sum of squares) and assign its fuzzy controller based on the feasibility of demonstration of a nonlinear system as an example.

Performance Improvement by Cluster Analysis in Korean-English and Japanese-English Cross-Language Information Retrieval (한국어-영어/일본어-영어 교차언어정보검색에서 클러스터 분석을 통한 성능 향상)

  • Lee, Kyung-Soon
    • The KIPS Transactions:PartB
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    • v.11B no.2
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    • pp.233-240
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    • 2004
  • This paper presents a method to implicitly resolve ambiguities using dynamic incremental clustering in Korean-to-English and Japanese-to-English cross-language information retrieval (CLIR). The main objective of this paper shows that document clusters can effectively resolve the ambiguities tremendously increased in translated queries as well as take into account the context of all the terms in a document. In the framework we propose, a query in Korean/Japanese is first translated into English by looking up bilingual dictionaries, then documents are retrieved for the translated query terms based on the vector space retrieval model or the probabilistic retrieval model. For the top-ranked retrieved documents, query-oriented document clusters are incrementally created and the weight of each retrieved document is re-calculated by using the clusters. In the experiment based on TREC test collection, our method achieved 39.41% and 36.79% improvement for translated queries without ambiguity resolution in Korean-to-English CLIR, and 17.89% and 30.46% improvements in Japanese-to-English CLIR, on the vector space retrieval and on the probabilistic retrieval, respectively. Our method achieved 12.30% improvements for all translation queries, compared with blind feedback in Korean-to-English CLIR. These results indicate that cluster analysis help to resolve ambiguity.