• Archives of Pharmacal Research
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• 제20권6호
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• pp.550-554
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• 1997

Chlorambucil is known to alkylate primarily N7 of guanine and N3 of adenine to induce DNA monofunctional adducts and interstrand cross-links (ISC). We have investigated the sequence specificity for DNA ISC induced by chlorambucil using duplex oligomers containing a defined cross-linkable sequences $ 5^{I}-A*TT, 5^{I}-G*TTor5^{I}-G*CC$ under bar which asterisk indicates the potential cross-linking site and underlined base indicates the potential cross-linking site on the opposite strand. An analysis of 20% denaturing polyacrylamide gel electrophoresis showed that chlorambucil was albe to induce DNA ISC in the duplex oligomers containing a sequence $5^I-GCC$. The formation of DNA ISC was not observed in the duplex oligomers containing sequences $5^I-ATT$. or $5^I-GTT$. These results indicate that chlorambucil induces guanine-guanine DNA ISC but not guanine-adenine or adenine-adenine DNA ISC. In addition, we have tested the ability of chlorambucil to induce DNA ISC within $5^I-GNNC$ or $5^I-GNNC$sequences using duplex oligomers containing the sequence$5^I-G^4G^3G^2^C$. The result of DNA strand cleavage assay showed that DNA ISC was formed at the $5^I-GGC$ sequence (an 1,3 cross-link, $G^1-G^3$) but not at $5^I-GGGC$ (an 1,4 cross-link, $G^1-G^4$) or $5^I-GC$ sequence (an 1,2 cross-link, $G^1-G^2$).

• Membrane and Water Treatment
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• 제6권3호
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• pp.173-187
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• 2015

The goal of present work is the preparation of a novel positively charged nanofiltration (NF) membrane and its development for the cation removal of aqueous solutions. This NF membrane was fabricated by the surface modification of polysulfone (PSf) ultrafiltration support. The active top-layer was formed by interfacial cross-linking polymerization of poly(ethyleneimine) (PEI) with p-xylylene dichloride (XDC) and then quaternized with methyl iodide to form a perpetually positively charged layer. In order to improve the efficiency of nanofiltration membrane, the concentration of PEI, XDC and methyl iodide solutions, PEI coating and cross-linking time have been optimized. As a result, a high water flux and high $CaCl_2$ rejection (1,000 ppm) was obtained for the composite membrane with values of $18.29L/m^2.h$ and 93.62% at 4 bar and $25^{\circ}C$, respectively. The rejections of NF membrane for different salt solutions followed the order of $Na_2SO_4$ < $MgSO_4$ < NaCl < $CaCl_2$. Molecular weight of cut off (MWCO) was calculated via retaining of PEG solutions with different molecular weights that finally, it revealed the Stokes and hydrodynamic radius of 1.457 and 2.507 nm on the membrane selective layer, respectively. The most efficient positively charged nanofiltration membrane exhibited a $Ni^{2+}$ rejection of 96.26% for industrial wastewater from Shamse Hadaf Co. (Kashan, Iran).

• 콘크리트학회논문집
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• 제23권4호
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• pp.471-478
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• 2011

콘크리트구조설계기준에서는 균열 검토시 설계의 간편함 때문에 철근 간격을 제한하는 간접 균열 제어 방법을 사용하고 있다. 뿐만 아니라 구조설계기준 부록에서는 균열폭을 산정하는 직접 균열 제어 방법도 제시하고 있다. 그러나 이러한 두 방법을 통한 균열 검증 결과에는 모순이 있다. 이 연구에서는 구조설계기준, 구조설계기준 부록 및 Frosch 제안식을 통해 최대 철근 간격을 산정하고, 콘크리트 압축강도, 단면 높이 그리고 피복 두께에 따른 차이를 분석하였다. 연구 결과 구조설계기준 본문과 구조설계기준 부록에 따른 최대 철근 간격에는 큰 차이가 있는 것으로 나타났다. 따라서 직접 균열 제어와 간접 균열 제어 사이에 일관성을 확보할 수 있는 합리적인 균열 검증 모델을 제안하였다.

• 한국강구조학회 논문집
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• 제24권6호
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• pp.701-710
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• 2012

최근에는 고층구조물이나 해양구조물(플랜트)의 경우 대부분 대형화로 설계 및 시공되고 있다. 대형구조물의 경우 주로 강재(steel)를 사용하여 설계하고 있다. 구조물 설계는 설계단계에서 최상의 부재를 선택하는 최적화를 적용함으로서 비용을 줄이는 경제적인 효과를 얻을 수 있다. 본 연구에서는 트러스와 프래임 구조물의 최적설계에 연속변수와 이산변수를 사용하여 최적화 하였고, 설계에서 사용된 이산자료는 규격화된 강재에 대한 자료와 데이터화된 이산자료를 사용하였다. 최적화는 이 모두에 적합한 이산최적화를 적용하였다. 최적화 기법으로는 유전자 알고리즘을 사용하였으며, 사용된 구조물은 10-Bar, 25-Bar 트러스와 1경간 2층, 1경간 7층 프레임 구조물이다. 설계시 제약조건은 고유진동수와 부재응력, 변위제약조건들이 적용되었다. 유전자 알고리즘에는 번식과장에서 엘리트 개체가 2번 사용되도록 하였으며, 제약조건 위반시 패널티를 사용하는 방법을 사용하였다.

• 콘크리트학회논문집
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• 제21권1호
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• pp.65-74
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• 2009

이형철근의 부식이 우려되는 경우, GFRP 보강근의 사용이 사용될 수 있다. 이 연구에서는 GFRP 보강근으로 보강된 총 36개 보 및 일방향 슬래브의 휨 실험을 수행하였다. 4종의 GFRP 보강근을 실험에 사용하였고, 보강근 직경은 13 mm이었다. 대부분의 실험체의 보강근은 중앙부에서 겹침이음되었다. 모든 보 및 슬래브는 4점재하 되었으므로, 이음부는 균일한 모멘트를 받도록 계획하였다. 실험변수는 이음길이, 피복두께 및 보강근 간격이었다. 보수적으로 부착강도를 평가하기 위하여 이음부에는 스터럽을 사용하지 않았다. 실험결과 보강근과 콘크리트 간 발생한 부착응력을 비선형 단면해석을 통하여 결정하였다. 2변수 선형 회귀분석을 사용하여 평균부착강도의 예측식을 유도하였다. 5% 분위수 개념을 사용하여 이음길이 설계식을 제안하였다. 이 연구의 결과로 이론적인 이음길이 설계식이 제안되었으며 결과를 ACI 440 정착설계식과 비교하였다.

• Physical Therapy Rehabilitation Science
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• 제9권4호
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• pp.287-294
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• 2020

Objective: This study aimed to investigate the effects of an ultrasound-based bar graph proportional to the quadriceps muscle thickness as a real-time visual feedback training tool in the elderly. Design: Cross-sectional study. Methods: Twenty-four elderly persons participated in this study and were randomly divided into three groups: oral training group (n=8, group 1), ultrasound imaging group (n=8, group 2), and graph group (n=8, group 3). In the pre condition, all participants performed maximal voluntary isometric contraction (MVIC) of the quadriceps with knee extension three times with oral training. In the post condition, group 1 performed MVIC of the quadriceps with oral training, group 2 performed MVIC of the quadriceps with real-time visual feedback using ultrasound imaging, and group 3 performed MVIC of the quadriceps with real-time visual feedback using a bar graph proportional to the quadriceps muscle thickness, three times for all groups. Muscle thickness, activity (mean, peak), tone, stiffness, logarithmic decrement, relaxation, and creep were measured in both conditions in all participants. Results: Visual feedback with a bar graph showed significant effects on muscle thickness, mean muscle activity, and peak muscle activity compared with oral training and visual feedback with ultrasound imaging (p<0.05). Conclusions: Isometric training of the quadriceps with real-time visual feedback using a bar graph proportional to the quadriceps muscle thickness may be more effective than other methods in improving muscle thickness and muscle activity. This study presented a tool that can help increase muscle thickness in the elderly.

• Safety and Health at Work
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• 제4권1호
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• pp.27-36
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• 2013

Objectives: Mental health has been shown to be linked with certain underlying physiological mechanisms. The objective of this cross sectional study was to investigate the relationship between depressive symptoms and brachial artery reactivity (BAR) in an understudied population: police officers. Methods: Participants were 351 police officers who were clinically examined in the Buffalo Cardio-Metabolic Police Stress (BCOPS) study. BAR was performed using standard B-Mode ultrasound procedures. Depressive symptoms were measured using the Center for Epidemiological Studies Depression (CES-D) scale. Mean values of the difference between the baseline and maximum diameters of the brachial artery were determined across three categories of CES-D score using the analysis of variance and the analysis of covariance. p-values for linear trends were obtained from linear regression models. Results: The mean age (${\pm}$ standard deviation) of all officers was $40.9{\pm}7.2$ years. Women had a slightly higher mean CES-D score than men ($8.9{\pm}8.9$ vs. $7.4{\pm}6.4$) and a slightly higher percentage increase of BAR than men (6.90 vs. 5.26%). Smoking status significantly modified the associations between depressive symptoms and BAR. Among current smokers, mean absolute values of BAR significantly decreased as depressive symptoms increased after adjustment for age, gender, race/ethnicity, hypertension, and diabetes; the multivariate-adjusted p-values were 0.033 (absolute) and 0.040 (%). Associations between depressive symptoms and BAR were not statistically significant among former smokers or never smokers. Conclusion: Depressive symptoms were inversely associated with BAR among police officers who were current smokers and together may be considered a risk factor for cardiovascular disease among police officers. Further prospective research is warranted.

• 대한임상검사과학회지
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• 제36권2호
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• pp.127-130
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• 2004

Analytical sensitivity on TDM test is the lowest concentration that can be distinguished from background noise. The aim of study was to evaluate analytical sensitivity that is also referred to as the lower limit of detection(LLD) about difference between zero calibrator and isotonic saline sample. We tested for 10 days with zero calibrators and 0.85% saline samples while running trilevel control samples under control. Raw data divided by two groups calculated mean and standard deviation from two sample populations and analytical sensitivity by ${\bar{X}}+2SD$. In comparison with isotonic saline samples and zero calibrators, there were significant differences in phenytoin, phenobarbital and vancomycin, etc. Especially analytical sensitivity on phenytoin is at the same level as the upper limit of analytical measurement range with $40{\mu}g/mL$. We think the cause of this is matrix interference. In conclusion, we were sure that standard protocol for analytical sensitivity as lower limit of analytical measurement range on TDM test must be measured with zero standard rather than an isotonic saline sample and type 1 reagent DW for reducing matrix effects within interactions between different materials in a mixture.

• Computers and Concrete
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• 제11권2호
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• pp.105-121
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• 2013

This paper investigates the parameters that control the design of Fiber Reinforced Polymer (FRP) reinforced concrete flexural members proportioned following the ACI 440.1R-06. It investigates the critical parameters that control the flexural design, such as the deflection limits, crack limits, flexural capacity, concrete compressive strength, beam span and cross section, and bar diameter, at various Mean-Ambient Temperatures (MAT). The results of this research suggest that the deflection and cracking requirements are the two most controlling limits for FRP reinforced concrete flexural members.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 1998년도 춘계학술대회 논문집
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• pp.258-262
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• 1998

A structural design based of finite element analysis has been performed to investigate the reliable automatic transport system. For the procedure, different cross-sectional geometric factors were calculated and compared in detail. The von-Mises stresses were chosen to maintain a solid loading braring capacity for the safe design. Parametric studies showed that it is more inportant for the plate bending problem than the vertical loads of hanging bar to assess the safe and economic structural design.