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Production of doubled haploid population derived from the microspore culture of rapeseed (Brassica napus L.) F1 generation and analysis of fatty acid composition (유채 잡종 1세대의 소포자 배양에 의한 배가반수체 집단 선발 및 지방산 조성 분석)

  • Lee, Ji Eun;Park, Ju Hyun;Kim, Kwang Soo;An, Da Hee;Cha, Young Lok
    • Journal of Plant Biotechnology
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    • v.49 no.1
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    • pp.74-81
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    • 2022
  • Brassica napus, an oil crop that produces rapeseed oil, is an allotetraploid (AACC, 2n = 38) produced by natural hybridization between B. rapa and B. oleracea. In this study, microspore was cultured using the F1 developed from a cross between 'EMS26' line with high oleic acid content and 'J8634-B-30' lines. The flower bud size showing the nuclear development at the late uninucleate and binucleate stage with high embryogenesis rate was 2.6 ~ 3.5 mm. Microspores were cultured using only this size and after then most microspore embryo developed into secondary embryos and then regeneration plants obtained from the developed multilobe. The analysis of the ploidy of the plants revealed that 66.7% and 27.8% of the total lines were tetraploids and octoploids, respectively. The sizes of stomatal cells in tetraploids, octoploids, and diploids were 25.5, 35.6, and 19.9 ㎛, respectively, indicating that ploidy level was positively correlated with cell size. Furthermore, 62 tetraploid doubled haploid (DH) lines were selected. The average oleic acid (C18:1) and linolenic acid (C18:3) concentrations of DH were 72.3% and 6.2%, respectively. Oleic acid and linolenic acid concentrations exceeded the two parental values in 5 and 14 DH lines, respectively, suggesting that these two fatty acids had transgressive segregation. Therefore, the DH population can be utilized for the biosynthesis of unsaturated fatty acids in rapeseed and related genes. It can also be used as a breeding material for varieties with high oleic acid concentrations.

A Study on the Retrieval of River Turbidity Based on KOMPSAT-3/3A Images (KOMPSAT-3/3A 영상 기반 하천의 탁도 산출 연구)

  • Kim, Dahui;Won, You Jun;Han, Sangmyung;Han, Hyangsun
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1285-1300
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    • 2022
  • Turbidity, the measure of the cloudiness of water, is used as an important index for water quality management. The turbidity can vary greatly in small river systems, which affects water quality in national rivers. Therefore, the generation of high-resolution spatial information on turbidity is very important. In this study, a turbidity retrieval model using the Korea Multi-Purpose Satellite-3 and -3A (KOMPSAT-3/3A) images was developed for high-resolution turbidity mapping of Han River system based on eXtreme Gradient Boosting (XGBoost) algorithm. To this end, the top of atmosphere (TOA) spectral reflectance was calculated from a total of 24 KOMPSAT-3/3A images and 150 Landsat-8 images. The Landsat-8 TOA spectral reflectance was cross-calibrated to the KOMPSAT-3/3A bands. The turbidity measured by the National Water Quality Monitoring Network was used as a reference dataset, and as input variables, the TOA spectral reflectance at the locations of in situ turbidity measurement, the spectral indices (the normalized difference vegetation index, normalized difference water index, and normalized difference turbidity index), and the Moderate Resolution Imaging Spectroradiometer (MODIS)-derived atmospheric products(the atmospheric optical thickness, water vapor, and ozone) were used. Furthermore, by analyzing the KOMPSAT-3/3A TOA spectral reflectance of different turbidities, a new spectral index, new normalized difference turbidity index (nNDTI), was proposed, and it was added as an input variable to the turbidity retrieval model. The XGBoost model showed excellent performance for the retrieval of turbidity with a root mean square error (RMSE) of 2.70 NTU and a normalized RMSE (NRMSE) of 14.70% compared to in situ turbidity, in which the nNDTI proposed in this study was used as the most important variable. The developed turbidity retrieval model was applied to the KOMPSAT-3/3A images to map high-resolution river turbidity, and it was possible to analyze the spatiotemporal variations of turbidity. Through this study, we could confirm that the KOMPSAT-3/3A images are very useful for retrieving high-resolution and accurate spatial information on the river turbidity.

A fundamental study on the automation of tunnel blasting design using a machine learning model (머신러닝을 이용한 터널발파설계 자동화를 위한 기초연구)

  • Kim, Yangkyun;Lee, Je-Kyum;Lee, Sean Seungwon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.24 no.5
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    • pp.431-449
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    • 2022
  • As many tunnels generally have been constructed, various experiences and techniques have been accumulated for tunnel design as well as tunnel construction. Hence, there are not a few cases that, for some usual tunnel design works, it is sufficient to perform the design by only modifying or supplementing previous similar design cases unless a tunnel has a unique structure or in geological conditions. In particular, for a tunnel blast design, it is reasonable to refer to previous similar design cases because the blast design in the stage of design is a preliminary design, considering that it is general to perform additional blast design through test blasts prior to the start of tunnel excavation. Meanwhile, entering the industry 4.0 era, artificial intelligence (AI) of which availability is surging across whole industry sector is broadly utilized to tunnel and blasting. For a drill and blast tunnel, AI is mainly applied for the estimation of blast vibration and rock mass classification, etc. however, there are few cases where it is applied to blast pattern design. Thus, this study attempts to automate tunnel blast design by means of machine learning, a branch of artificial intelligence. For this, the data related to a blast design was collected from 25 tunnel design reports for learning as well as 2 additional reports for the test, and from which 4 design parameters, i.e., rock mass class, road type and cross sectional area of upper section as well as bench section as input data as well as16 design elements, i.e., blast cut type, specific charge, the number of drill holes, and spacing and burden for each blast hole group, etc. as output. Based on this design data, three machine learning models, i.e., XGBoost, ANN, SVM, were tested and XGBoost was chosen as the best model and the results show a generally similar trend to an actual design when assumed design parameters were input. It is not enough yet to perform the whole blast design using the results from this study, however, it is planned that additional studies will be carried out to make it possible to put it to practical use after collecting more sufficient blast design data and supplementing detailed machine learning processes.

A Study on Cinematic Representations of Posthuman Girls in South Korea-Focused on The Silenced and The Witch: Part 1. The Subversion (한국 영화에 나타난 포스트휴먼 소녀의 재현 양상 연구 -<경성학교: 사라진 소녀들>, <마녀>를 중심으로)

  • Kim, Eun Joung
    • Journal of Popular Narrative
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    • v.27 no.3
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    • pp.95-124
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    • 2021
  • As the symbolic images of girls besides its definition have varied according to the age and society, a posthuman girl character recently appears in the digital cinema. This study aims to analyze its cinematic representations and the social contexts in which they are created. For this purpose, the study focuses on what extent the society allows its imagined figurations as a future female body and the meanings revolving around the image of 'technologically body-enhanced female fighter'. Current digital visualization technology has developed to the extent any imaged future humans can be represented, but posthuman girls' representations have its limitation that only a human-like figuration can be allowed in accord with the traditionally idolized image of girls. It is because of the representation logic in which digital cinema is visualized based on perceptual realism that values audiences' experiences. Despite such less critical figuration which does not dare to cross the boundary between the image of human and inhuman, the posthuman girl characters create a new category of the 'dangerous girls' who are both void of sexual femininity and independent of motherhood and heterosexual romance narrative. Of course, they support the modern human-centered belief that humans can take entire control of technology with their moral behaviors and dispel the fear about the negative impact the nature of technology may have on society at large by showing their child-like figuration protecting ethical values. However, the new character of 'unruly girl' exerts her subversive act that seeks to fight against the human-centered liberal humanistic values and melancholic feeling and vulnerability that the neoliberalism and technocracy enforce. When posthuman girl characters are considered to be a marker through which we can see how different social forces are intervening and competing each other in the upcoming posthuman age, the limited figuration of the posthuman girl characters in South Korean movies illustrates the opinionated thoughts toward the instrumentalism in technology but their bloodshed struggles reveal how the corporate or state-governed techno-biopower has oppressively treated and appropriated the human body as the technology-object and also provide a meaningful opportunity to rethink its unethical violence.

(Im)Mobility as Dispositif and its Representations - Mobility-Based Textual Research Method Centered on Mobility and Foucault (장치로서의 (임)모빌리티와 그 재현 -『모빌리티와 푸코』를 중심으로 한 텍스트 연구 시론)

  • Kim, Na-Hyun
    • Journal of Popular Narrative
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    • v.27 no.3
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    • pp.195-228
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    • 2021
  • The purpose of this study is to review the mobility-based textual research methods raised in Mobility and Foucault and apply them to textual analysis. This book contains seven articles applying Foucault's terms to mobility studies, giving intellectual stimulation to both studies. Since Foucault examined discipline power operated through the technology of distinguishing between rational/irrational and normal/abnormal, his works seem to a study of closed spaces like prisons. However, the authors of this book note that Foucault's works already had sufficient insight on mobility, and them actively incorporated it into mobility study. When we concentrate Foucault's works on mobility as a governmentality and a dispositif, the tension and dynamics between mobility and immobility are emphasized. And then it is possible to cross the simple dichotomy in mobility studies. This paper analyzes Kim Joong-hyuk's short story 1F/B1 by applying this method. This story describes a building manager who seems to be fixed in a building, but the mobility of him in the story goes through stereotypes and creates new spaces. Kim Hye-jin's short stories also represent mobility that cannot move and hesitates. These stories are important in that they show the mobility as a dispositif that constitutes the subject. When referring to the achievements of Mobility and Foucault, we read this narrative again by paying attention to the dynamics of mobility and immobility in the text. The significance of this paper is that it expands mobility-based textual research anew. While text analysis applying mobility study was usually focused on clearly mobile narratives such as travel statements and diaspora narratives, Mobility and Foucault drives new textual research by paying attention to the relationship between power and mobility, mobility and immobility dynamics. Therefore, this paper is significant in confirming the new meaning of the text revealed when paying attention to the representation of mobility in the narrative that no one seems to be mobile, and seeking to expand the mobility-based textual research method.

Interpretation of Firing Temperature and Thermal Deformation of Roof Tiles from Ancient Tombs of Seokchon-dong in Seoul, Korea (서울 석촌동 고분군 출토 기와의 소성온도와 열변형 특성 해석)

  • Jin, Hong Ju;Jang, Sungyoon;Lee, Myeong Seong
    • Economic and Environmental Geology
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    • v.54 no.6
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    • pp.671-687
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    • 2021
  • This study investigated the firing temperature and thermal deformation process of roof tiles excavated from the connected stone-mound tomb in Seokchon-dong, Seoul, based on mineralogical and physical properties. A large number of roof tiles were excavated from the tomb site and some roof tiles were deformed by heat and were fired in uneven conditions. The colors of original roof tiles and their cores are mostly yellowish-brown, with high water absorption over 12%, containing fine-grained textures and some minerals such as quartz, feldspars, amphibole, and mica. It is estimated that the original roof tiles were fired below 900℃ in oxidation condition, showing loose matrices and mica layers by scanning electron microscopy. However, deformed roof tiles have the uneven surface color of reddish-brown and bluish-gray, and those cross-sections have sandwich structures in which dense reddish-brown surface and porous grey core coexist. They contained mullite and hercynite, so it was estimated to have been fired over 1,000℃, with 0.81~11% water absorption. In some samples, bloating pores by overfiring were observed, which means that they were fired at more than 1,200℃. In addition, the refirng experiments that the original roof tile was fired between 800℃ and 1,200℃ were carried out to investigate the physical and mineralogical properties of roof tiles compared to deformed ones. As a result, the water absorption decreased rapidly and the mineral phase started to change over 1,000℃. As the temperature gradually rises, the matrices are partially melted and recrystallized, resulting in similar thermal characteristics of deformed roof tiles. Therefore, the roof tiles from ancient tombs in Seokchon-dong seem to experience the secondary high temperature of 1,000 to 1,200℃ under uneven firing conditions, resulting in deformation characteristics such as shape transformation and mineral phase transition. It is considered to have been related to cremation rituals at the tombs of Seockchon-dong during the Baekje period.

Analysis of the Effect of Entry-Level 3D Printer Materials on CT Images (보급형 3D프린터 재료가 CT 영상에 미치는 영향 분석)

  • Se-Hwan, Park;Hyun-Jung, Jo;Sung-Jun, Lee;Song-Bin, Lee;Sang-Hyub, Park;Dae-Yeon, Ryu;Yeong-Cheol, Heo
    • Journal of the Korean Society of Radiology
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    • v.16 no.6
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    • pp.673-680
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    • 2022
  • In this study, based on PLA, we analyzed the Hounsfield Unit (HU) of materials containing 20% each of aluminum, wood, copper, carbon, and marble, and tried to analyze how they affect the image. A cylindrical phantom of 5×30×30 ㎣ (thickness×diameter×height) was fabricated using a entry-level 3D printer. The kV was changed to 80, 100 and 120, and the mAs was changed to 100 and 200 mAs, and the phantom in the center of the table was cross-scanned under a total of six conditions. A circular ROI was set using image J program and the quantification value of the material part HU and the quantification value of the peripheral part CNR were obtained. The HU average of the material part increased in the order of [PLA - wood 20%], [PLA - marble 20%], [PLA - carbon 20%], [PLA 100%], [PLA - aluminum 20%], [PLA - copper 20%] (p<0.05) a negative correlation was confirmed with the HU by increasing kV. It was confirmed that the CNR value in the peripheral area increased in the order of [PLA - marble 20%], [PLA - copper 20%], [PLA - carbon 20%], [PLA - wood 20%], [PLA - aluminum 20%], and [PLA - 100%] (p<0.05). Human organs with similar HU values for each material are [PLA - copper 20%] compact bone, [PLA - aluminum 20%] cancellous bone, [PLA 100%] coagulated blood, [PLA - carbon 20%] and [PLA - marble 20%] liver, muscle, spleen and [PLA - wood 20%] had similar values to fat. In addition, we confirmed the blur phenomenon that blurs the image around the filament with all materials, and confirmed that [PLA 100%] especially has the most blur around the filament. Therefore, it is considered desirable to reflect the HU value of the target organ and consider cloudiness around the phantom when selecting materials for medical phantom fabrication, and this research can provide basic data.

Estimation of ecological flow and fish habitats for Andong Dam downstream reach using 1-D and 2-D physical habitat models (1차원 및 2차원 물리서식처 모형을 활용한 안동댐 하류 하천의 환경생태유량 및 어류서식처 추정)

  • Kim, Yongwon;Lee, Jiwan;Woo, Soyoung;Kim, Soohong;Lee, Jongjin;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.55 no.12
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    • pp.1041-1052
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    • 2022
  • This study is to estimate the optimal ecological flow and analysis the spatial distribution of fish habitat for Andong dam downstream reach (4,565.7 km2) using PHABSIM (Physical Habiat Simulation System) and River2D. To establish habitat models, the cross-section informations and hydraulic input data were collected uisng the Nakdong river basic plan report. The establishment range of PHABSIM was set up about 410.0 m from Gudam streamflow gauging station (GD) and about 6.0 km including GD for River2D. To select representative fish species and construct HSI (Habitat Suitability Index), the fish survey was performed at Pungji bridge where showed well the physical characteristics of target stream located downstream of GD. As a result of the fish survey, Zacco platypus was showed highly relative abundance resulting in selecting as the representative fish species, and HSI was constructed using physical habitat characteristics of the Zacco platypus. The optimal range of HSI was 0.3~0.5 m/s at the velocity suitability index, 0.4~0.6 m at the depth suitability index, and the substrate was sand to fine gravel. As a result of estimating the optimal ecological flow by applying HSI to PHABSIM, the optimal ecological flow for target stream was 20.0 m3/sec. As a result of analysis two-dimensional spatial analysis of fish habitat using River2D, WUA (Weighted Usable Area) was estimated 107,392.0 m2/1000 m under the ecological flow condition and it showed the fish habitat was secured throughout the target stream compared with Q355 condition.

Nonlinear Vector Alignment Methodology for Mapping Domain-Specific Terminology into General Space (전문어의 범용 공간 매핑을 위한 비선형 벡터 정렬 방법론)

  • Kim, Junwoo;Yoon, Byungho;Kim, Namgyu
    • Journal of Intelligence and Information Systems
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    • v.28 no.2
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    • pp.127-146
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    • 2022
  • Recently, as word embedding has shown excellent performance in various tasks of deep learning-based natural language processing, researches on the advancement and application of word, sentence, and document embedding are being actively conducted. Among them, cross-language transfer, which enables semantic exchange between different languages, is growing simultaneously with the development of embedding models. Academia's interests in vector alignment are growing with the expectation that it can be applied to various embedding-based analysis. In particular, vector alignment is expected to be applied to mapping between specialized domains and generalized domains. In other words, it is expected that it will be possible to map the vocabulary of specialized fields such as R&D, medicine, and law into the space of the pre-trained language model learned with huge volume of general-purpose documents, or provide a clue for mapping vocabulary between mutually different specialized fields. However, since linear-based vector alignment which has been mainly studied in academia basically assumes statistical linearity, it tends to simplify the vector space. This essentially assumes that different types of vector spaces are geometrically similar, which yields a limitation that it causes inevitable distortion in the alignment process. To overcome this limitation, we propose a deep learning-based vector alignment methodology that effectively learns the nonlinearity of data. The proposed methodology consists of sequential learning of a skip-connected autoencoder and a regression model to align the specialized word embedding expressed in each space to the general embedding space. Finally, through the inference of the two trained models, the specialized vocabulary can be aligned in the general space. To verify the performance of the proposed methodology, an experiment was performed on a total of 77,578 documents in the field of 'health care' among national R&D tasks performed from 2011 to 2020. As a result, it was confirmed that the proposed methodology showed superior performance in terms of cosine similarity compared to the existing linear vector alignment.

Literature review and future tasks necessary to establish of Korean Dietary Reference Intake for choline (콜린의 한국인 영양소 섭취기준 제정 검토를 위한 문헌 고찰 및 향후 과제)

  • Shim, Eugene;Park, Jae-Hee;Lee, Yunjung;Park, Eunju
    • Journal of Nutrition and Health
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    • v.55 no.2
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    • pp.211-226
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    • 2022
  • Choline, an essential nutrient for humans, is required for the structural integrity of the cell membranes, methyl-group metabolism, synthesis of the neurotransmitter acetylcholine, synthesis of the membrane phospholipid components of the cell membranes, and the transport of lipids and cholesterol. Choline can be synthesized in the body, but it is insufficient to meet the daily requirements and hence it must be obtained through the diet. In the United States/Canada, Australia/New Zealand, Europe, China, and Taiwan, the adequate intake (AI) and tolerable upper intake level (UL) of choline have been established, while the establishment of the 2020 Dietary Reference Intakes for Koreans (KDRI) for choline was postponed due to the lack of a choline database for Korean foods and studies on the choline intake of Koreans. However, as part of the preparation work for the 2020 DRI revision and finalization, choline intake and the possibility of disease occurrence were verified through analysis of published data. The groundwork for the subsequent establishment of a choline DRI was laid through a literature search, evaluation, and review of the literature reported from 1949 up to 2019. This can be regarded as the culmination of this project. According to the results of randomized controlled trials (RCTs), cohort studies, case-control studies, and cross-sectional observational studies in humans, approximately 400-500 mg/day of choline intake was effective in preventing liver function damage (fatty liver), neural tube damage, cardiovascular disease, breast cancer, and cognitive function improvement. The same amount of choline intake, however, also correlated with the risk of prostate and colorectal cancer. At present, there is limited information available on choline intake and health outcomes, particularly for the Korean population. More human studies, including clinical trials on the requirements and the physiological benefits associated with dietary intake, are needed to establish the KDRI for choline.