Lee, Ka Soon;Kim, Gwan Hou;Seong, Bong Jae;Kim, Sun Ick;Han, Seung Ho;Lee, Sox Su;Yang, Hui;Yoo, Yung Choon
Korean Journal of Medicinal Crop Science
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v.22
no.6
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pp.449-456
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2014
Anti-inflammatory activity of the extracts of ginseng berry (GBE) was investigated through the evaluation of its inhibitory effect on the production of inflammatory meditator, nitric oxide(NO), tumor necrocis factor-alpha (TNF-${\alpha}$), interleukin-6 (IL-6) in LPS-induced RAW264.7 macrophage cells. GBE was fractionated using n-hexane, chloroform, ethylacetate, buthanol and $H_2O$, sequentially. RAW264.7 cells were induced $100ng/m{\ell}$ of lipopolysaccharide (LPS) and treated with 0, 1.6, 8, 40 and $200{\mu}g/m{\ell}$ of GBE fractions. LPS-induced NO production on all of GBE fractions was inhibited with increasing added concentration of GBE fractions. Chloroform fraction of GBE was the most effective in inhibiting LPS-induced TNF-${\alpha}$ production. Hexane, chloroform and $H_2O$ fractions of GBE exhibit strong inhibition LPS-induced IL-6 production. Especially, $H_2O$ fractions of GBE was the most effective in inhibiting LPD-induced IL-6 production without significant cytotoxicity in RAW264.7 cells, and reduced the activation of mitogen-activated protein kinases (MAPK) and IkB phosphorylation. These results indicate that $H_2O$ fractions of GBE exhibits strong anti-inflammatory effects by inhibition of NF-kB by inhibition of p-38 on MAPK and IkB phosphorylation.
Dea-Wook Kim;Yu Young Lee;Hyeonsoo Jang;Yun-Ho Lee;Jong Tag Youn;Hee Woo Lee;Hak Yong Lee;Young Mi Park
Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.183-183
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2022
This study was aimed to investigate the content of avenanthramides(AVNs) and other phenolic compounds in the grains of a naked oat cultivar, Daeyang, which were seeded in the fall and spring, and examine the effects of alcohol extracts from the grains on natural killer(NK) cell activity in vitro. The content of AVN-A, AVN-B, and AVN-C in the spring-seeded oat grains was 2.2 folds higher than the fall-seeded oat grains on average. Among these AVNs, the content of AVN-C was 1.9-folds higher in the spring-seeded oat grains(66.1㎍/g), comparing to the content in the fall-seeded oat grains(34.8㎍/g). The content of other phenolic compounds, such as phenolic acids and flavonoids in the spring-seeded oat grains was 1.1~4.7-folds higher than the fall-seeded oat grains. In particular, sinapinic acid was the most abundant phenolic acid in the spring-seeded oat grains(50.0㎍/g) and its content was 2.4-folds higher than its content in the fall-seed oat grains. Furthermore, NK cell activity in vitro treated with the spring-seeded oat grain extracts was 158%, and it was 18%p higher than NK cell activity treated with the extracts from the fall-seeded oat grain extracts. Our finding suggest that the bioactive properties of naked oat grains would be enhanced by spring seeding.
Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.202-202
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2022
Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r2=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.
The frequency and intensity of soil moisture stress associated with climate change has increasing, and the stability of field crop cultivation has decreasing. This experiment was conducted to investigate the effect of soil moisture management method on growth and yield of corn. Soil moisture was managed at the grade of WSM (wet soil moisture, 34.0~42.9%), OSM (optimum soil moisture, 27.8~34.0%), DSM (dry soil moisture, 20.3~27.8%), and ESM (extreme dry moisture, 16.6~20.3%) during V8 (8th leaf stage)-VT (tasseling stage). After VT, irrigation was limited. The treated amount of irrigation was 54.1, 47.7, 44.0 and 34.5% of total water requirement, respectively. The potential evapotranspiration during the growing period was $3.29mm\;day^{-1}$, and upward movement of soil water was estimated by the AFKAE 0.5 model in the order of ESM, DSM, OSM, and WSM. We could confirm this phenomenon from actual observations. There was no significant difference in leaf characteristics, dry matter, and primary productivity depending on the level of soil moisture, but leaf development was delayed and dry weight decreased in DSM. However, dry weight and individual productivity of DSM increased after irrigation withdrawal compared to that of OSM. In DSM, ear yield and number of kernels per ear decreased, but water use efficiency and harvest index were higher than other treatments. Therefore, it is considered that the soil moisture is concentratedly managed before the V8 period, the V8-VT period is controlled within the range of 100 to 500 kPa (20.3~27.8%), and no additional irrigation is required after the VT.
Lim, Chul-Hee;Kim, Gang Sun;Lee, Eun Jung;Heo, Seongbong;Kim, Teayeon;Kim, Young Seok;Lee, Woo-Kyun
Journal of Climate Change Research
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v.7
no.2
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pp.193-203
/
2016
The importance of forecasting agricultural production is receiving attention while climate change is accelerating. This study suggested three types of crop yield forecasting model for major vegetable crops by using downscaled meteorological information of main production area on farmland level, which identified as limitation from previous studies. First, this study conducted correlation analysis with seven types of farm level downscaled meteorological informations and reported crop yield of main production area. After, we selected three types of meteorological factors which showed the highest relation with each crop species and regions. Parameters were deducted from meterological factor with high correlation but crop species number was neglected. After, crop yield of each crops was estimated by using the three suggested types of models. Chinese cabbage showed high accuracy in overall, while the accuracy of daikon and onion was quiet revised by neglecting the outlier. Chili and garlic showed differences by region, but Kyungbuk chili and Chungnam, Kyungsang garlic appeared significant accuracy. We also selected key meteorological factor of each crops which has the highest relation with crop yield. If the factor had significant relation with the quantity, it explains better about the variations of key meteorological factor. This study will contribute to establishing the methodology of future studies by estimating the crop yield of different species by using farmland meterological information and relatively simplify multiple linear regression models.
Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.13-13
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2022
Recently, stable crop production is threatened by the effects of climate change. In particular, it is difficult to consistently maintain agricultural policies due to large price fluctuations depending on the difference in total domestic rice production from year to year. For stable rice production amid changes in the crop growing environment, development of varieties with improved disease resistance and abiotic stress stability is becoming more important. In here, drought and cold tolerant trait have been studied. First, for the development of drought tolerant varieties, we analyzed which agricultural traits are mainly affected by domestic drought conditions. As a result, it was observed that drought caused by the lack of water during transplanting season inhibits the development of the number of tiller and reduces the yield. 'Samgang' was selected as a useful genetic resource with strong drought tolerant and stable tiller number development even under drought conditions by phenotype screening. Three of drought tolerant QTLs were identified using doubled haploid (DH) population derived from a cross between Nacdong and Samgang, a drought sensitive and a tolerant, respectively. Among these QTLs, when qVDT2 and qVDTl1 were integrated, it was investigated that the tiller number development was relatively stable in the rainfed paddy field conditions. It is known that the high-yielding Tongil-type cultivars are severely affected by cold stress throughout the entire growth stage. In this study, we established conditions that can test the cold tolerance phenotype with alternate temperature to treat low temperatures in indoor growth conditions similar to those in field conditions at seedling stage. Three cold tolerant QTLs were explored using population derived from a cross between Hanareum2 (cold sensitive variety, Tongil-type) and Unkwang (cold tolerant variety, Japonica). Among these QTLs, qSCT12 showed strong cold tolerant phenotype, and when all of three QTLs were integrated, it was investigated that cold tolerant score was relatively similar to its donor parent, Unkwang, in our experimental conditions. We are performing that development of new variety with improved cold tolerant through the introduction of these QTLs.
Korean Journal of Agricultural and Forest Meteorology
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v.22
no.1
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pp.13-19
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2020
This study compared RGB color images with canopy light interception (LI) and leaf area index (LAI) measurements for low cost and low labor. LAI and LI were measured from vertical gap fraction derived from top of digital image in soybean canopy cover (cv Daewonkong, Deapongkong and Pungsannamulkong). RGB color images, LAI, and LI were collected from V4.5 stage to R5stage. Image segmentation was based on excess green minus excess red index (ExG-ExR). There was a linear relationship between LAI measured with LI (r2=0.84). There was alinear relation ship between LI measured with canopy cover on image (CCI) (r2=0.94). There was a significant positive relationship(r2=0.74) between LAI and CCI at all grow ingseason. Therefore, it is expected that in the future, the RGB color image could be able to easily measure the LAI and the LI at low cost and low labor.
Human influence on soil formation has dramatically increased with human civilization and industry development. Increase of anthropogenic soils induced researches on the anthropogenic soils; classification, chemical and physical characteristics of anthropogenic soils and plant growth from anthropogenic soils. However there have been no comprehensive analyses on soil pore or physical properties of anthropogenic soils from 3 dimensional images in Korea. The objectives of this study were to characterize physical properties of anthropogenic paddy field soils by depth and to find differences between natural and anthropogenic paddy field soils. Soil samples were taken from two anthropogenic and natural paddy field soils; anthropogenic (A_c) and natural (N_c) paddy soils with topsoil of coarse texture and anthropogenic (A_f) and natural (N_f) paddy soils with topsoil of fine texture. The anthropogenic paddy fields were reestablished during the Arable Land Remodeling Project from 2011 to 2012 and continued rice farming after the project. Natural paddy fields had no artificial changes or disturbance in soil layers up to 1m depth. Samples were taken at three different depths and analyzed for routine physical properties (texture, bulk density, etc.) and pore properties with computer tomography (CT) scans. The CT scan provided 3 dimensional images at resolution of 0.01 mm to calculate pore radius size, length, and tortuosity of soil pores. Fractal and configuration entropy analyses were applied to quantify pore structure and analyze spatial distribution of pores within soil images. The results of measured physical properties showed no clear trend or significant differences across depths or sites from all samples, except the properties from topsoils. The results of pore morphology and spatial distribution analyses provided detailed information of pores affected by human influences. Pore length and size showed significant decrease in anthropogenic soils. Especially, pores of A_c had great decrease in length compared to N_c. Fractal and entropy analyses showed clear changes of pore distributions across sites. The topsoil layer of A_c showed more degradation of pore structure than that of N_c, while pores of A_f topsoil did not show significant degradation compared with those of N_f. These results concluded that anthropogenic soils with coarse texture may have more effects on pore properties than ones with fine texture. The reestablished paddy fields may need more fundamental remediation to improve physical conditions.
After ten years of implementing the agricultural automation program in Taiwan, some positive effects and satisfactory results have been recognized by both the agricultural industry and local administrative bureaux. The automation of agriculture is a response to sophisticated demands for production and quality in countries with high labor costs. The development of sensor systems, control systems, precision agriculture systems, and engineering for plant culture systems will determine the degree of automation used for crop production in the 21st century. The engineering system will capitalize upon expertise from physiologists, pathologists, systems analysts, agronomists, horticulturists, computer programmers, economists, crop producers and managers in order to efficiently implement automated crop production.
Soil conservation management is necessary for sustainable agriculture, in highland areas, and cover crops are one of the best soil conservation methods for slopes. In this study, we evaluated the effects of alfalfa cultivation on maize production, as well as soil conservation and quality. There was an outstanding soil conservation effect with alfalfa cultivation in the fallow and maize growing seasons. In particular, alfalfa cultivation reduced soil loss by up to 98% compared with bare field. It also increased the activities of soil microorganisms and the supply of organic matter. Maize production with alfalfa cultivation showed no significant differences in yield. In conclusion, alfalfa is an advantageous perennial cover crop in highland agricultural slope areas, which can have positive effects on soil quality and conservation, as well as maize production.
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