• Proceedings of the Korean Society of Grassland Science Conference
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• 2002.09b
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• pp.131-156
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• 2002

Although economically viable, the livestock industry is currently facing a number of challenging environmental problems and highly complex social issues, many of which are related to its size and geographically concentrated nature. Increased emphasis on environmental quality has also placed new demands on livestock producers to ensure that their production practices are in harmony with natural environment. In terms of sustainable agricultural systems, ruminants have served and will continue to serve a valuable role. They are particularly useful in converting vast renewable resources from rangeland, pasture, and crop residues or other by-product into food edible for human. With ruminants, land that is too poor or too erodable to cultivate becomes productive. Also, nutrients in by-products are utilized and do not become a waste-disposal problem. In Korea, however, native and dairy cattle production is not consistent with the advantageous roles of ruminant livestock in sustainable agricultural system because imported feed grains become the main basis for cattle raising. At present the ruminant livestock producers are heavily concentrated in and around the urban areas. About 75% of all the nation's cattle herds are kept on the outskirts of urban areas. As a result, the amount of pasture and forage land available per head of cattle is generally small. Furthermore, animals are raised in a cattle shed with high density. This situation is rather unfavorable for the national economic and environmental points of view As nation income increased, the demand for livestock products grew at an unforeseeable pace. But the pasture area involving in current utilization is tended to decrease during recent years. Based on the above figures more than 250,000 ha of pasture ought to be available for the present herd of cattle. It is obvious that these needs can scarcely be met with arable lands. Lands area for the establishment of new grassland have to be found in the hills and mountains which have not yet been used for crop framing or livestock. The development of extensive grasslands in the hill and woodland areas is now a declared aim. The starting point of the present work is the lack of knowledge of forage production and utilization in hill pasture and woodland in spite of indispensable necessity for livestock production in Korea. The importance of pastoral system in hill region and woodland is particularly emphasized in a standpoint of sustainable livestock production. Main chapter comprises the principle and techniques applicable for improving the utilization of hill pasture and woodland. We finally discussed the problems to solve and future works for a successful livestock production in hill and mountainous area in Korea.

• Korean Journal of Weed Science
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• v.13 no.4
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• pp.234-249
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• 1993

Herbicide is an essential agricultural chemical in the modern agriculture. Due to its bioactivity, however, risk of herbicide use against non-target organisms should be seriously considered. Among the unfavorable aspects given by herbicide, the residue is the most important because herbicide residue in soil and agricultural product is closely related to human safety. The residue in soil and crop is dependent on conditions of soil, weather, herbicide use and crop cultivation, etc. In general, the residue in soil or agricultural product in Korea is known to be not serious at this moment, except for some problems like carry-over effect on succeeding crops. To secure safety of herbicide use for the health, soil ecology and other environment, researches on herbicide residue including monitoring survey should be done more frequently and extensively. Safety guide for herbicide usage should be kept by farmers and development of long toxic herbicide should be accelerated.

• Korean Journal of Environmental Agriculture
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• v.37 no.1
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• pp.57-65
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• 2018

BACKGROUND: An analytical method was developed using HPLC-UVD/MS to precisely determine the residue of flusulfamide, a benzenesulfonamide fungicide used to inhibit spore germination. METHODS AND RESULTS: Flusulfamide residue was extracted with acetone from representative samples of five raw products which comprised apple, green pepper, Kimchi cabbage, hulled rice, and soybean. The extract was diluted with large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. For the hulled rice and soybean samples, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized Florisil column chromatography. On an octadecylsilyl column in HPLC, flusulfamide was successfully separated from co-extractives of sample, and sensitively quantitated by ultraviolet absorption at 280 nm with no interference. Accuracy and precision of the proposed method was validated by the recovery experiment on every crop sample fortified with flusulfamide at 3 concentration levels per crop in each triplication. CONCLUSION: Mean recoveries ranged from 82.3 to 98.2% in five representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation (LOQ) of flusulfamide was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring technique was also provided to clearly identify the suspected residue.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.24-34
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• 2020

Objective: The effects of Pleurotus ostreatus on the feed utilization of broad bean stalks (BBS), rape straw (RS), paddy straw (PS), and corn stalk (CS) was examined. Methods: The four roughages were co-cultured with Pleurotus ostreatus. The chemical composition; enzyme activities of laccase, carboxymethylcellulase (CMCase) and xylanase; carbohydrate and protein fractions (based on The Cornell Net Carbohydrate and Protein System [CNCPS]) were assessed at different days after inoculation (7, 14, 21, 28 d) and un-inoculated roughages (control, 0 d). The digestibility of nutrient components and the gas production of roughage with various incubation times were monitored at 0, 2, 4, 6, 9, 12, 24, 36, 48, 60, and 72 h using an in vitro ruminal fermentation method. Results: A higher CMCase activity (0.1039 U/mL) and earlier time to peak (14 d) were detected in Pleurotus ostreatus cultured with CS (p<0.05). Significantly, the incubation length-dependent responses of cumulative gas production were observed from 24 to 72 hours post fermentation (p<0.05), and these incubation length-dependent effects on cumulative gas production of PS and CS appeared earlier (24 h) for PS and CS than those (48 h) for BBS and RS (p<0.05). The fast-degradable carbohydrate (CA) content for all four roughages significantly increased over time (p<0.05). Nonetheless, increased degradation efficiency for CA treated with Pleurotus ostreatus was detected at both 21 and 28 days of incubation (p<0.05). With the exception of PS (p<0.05), there were no significant difference among the roughages (p>0.05) in slowly-degradable carbohydrate (CB2) at different incubation times (p<0.05). Conclusion: Assessment of the alterations in chemical composition, CNCPS system fractions, and the fermentation kinetics after biological pretreatment may yield a valuable database for evaluating the biological pretreatment of Pleurotus ostreatus in ruminant feed.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.309-317
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• 2015

BACKGROUND: An analytical method was developed using GC-ECD/MS to precisely determine the residue of fipronil, a phenylpyrazole insecticide used to control a wide range of foliar and soil-borne pests.METHOD AND RESULTS: Fipronil residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, Kimchi cabbage, green pepper, and apple. The extract was diluted with saline water, and fipronil was partitioned into n-hexane/dichloromethane (20/80, v/v) to remove polar co-extractives in the aqueous phase. Florisil column chromatography was additionally employed for final purification of the extract. Fipronil was separated and quantitated by GC-ECD using a DB-17 capillary column. Accuracy of the proposed method was validated by the recovery from crop samples fortified with fipronil at 3 levels per crop in each triplication.CONCLUSION: Mean recoveries ranged from 86.6% to 106.0% in five representative agricultural commodities. The coefficients of variation were less than 10%. Limit of quantitation of fipronil was 0.004 mg/kg as verified by the recovery experiment. A confirmatory technique using GC/MS with selected-ion monitoring was also provided to clearly identify the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fipronil in agricultural commodities.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.182-187
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• 2003

In order to elucidate a degradation characteristics of herbicide mefenacet in soil, the persistence in soils was studied under laboratory conditions for $90\sim120$ days at $28^{\circ}C$. Mefenacet residues were determined from the two soils which pre-treated by sterilization and flooding, respectively. Non-sterilized upland soil was used as a control. When 70 days elapsed from application time, $55\sim63%$ of mefenacet applied were dissipated in control soils. However, $32\sim33%$ of mefenacet applied were dissipated in the sterilized soils and $33\sim35%$ was dissipated in the flooded soils. 까 lese results indicated that the degradation of mefenacet was assumed to be due to microorganism, especially aerobic microbes. In order to elucidate the influence of water content on the persistence of mefenacet in soil, water content in soils was adjusted to 20, 50, and 80% of the water-holding capacity(Field capacity, WHC). The half-life of mefenacet in soil containing 20% and 50% of WHC were 82 and 73 days, respectively, after incubation for 90 days. However, the half-life in soil containing 80% of WHC was shortened to 61 days. These results indicated that degradation of mefenacet in soil was influenced by the activity of soil microorganism, organic matter content and water content.

• Food Science and Preservation
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• v.14 no.2
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• pp.124-130
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• 2007

Lettuce (Lactuca Sativa L.) is the most popular salad vegetable crop The purpose of this study was to analyze the quality of lettuce, and residual pesticides in this vegetable, during growth after transplanting. The eatable weight was 40% of the total weight at the commencement of transplanting, but had doubted at harvest after transplanting. As lettuce grew after transplanting, the pH, the sugar level, and the moisture content increased slightly, and the color tended towards yellow rather than greenThe SPDA chlorophyll measurement was 16.84 at 42 day post-ransplantation, and increased to 26.44 at 67 day after transplanting. The contents of vitamin C were 2.5mg/100g wet weight at 60 d after transplantation, but decreased to 2.2mg/100g wet weight 67 day post-transplantation. Mineral (Ca, Fe, K, Mg, and Na) levels rose after transplantation, and attained their highest levels 60 day post-transplantation. The lettuce crop was analyzed for residues of each of 48 pesticides nt 54 after transplantation, and the only such chemical detected was chlorthalonil(0.03 0.25mg/kg wet weight).

• Korean Journal of Environmental Agriculture
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• v.14 no.2
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• pp.186-201
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• 1995

In order to elucidate the fate of the residues of the pyrethroid acaricide-insecticide, acrinathrin in soil, maize plants were grown for one month on the specially-made pots filled with two different types of soils containing fresh and one-month-aged residues of [$^{14}C$]acrinathrin, respectively. The mineralization of [$^{14}C$]acrinathrin to $^{14}CO_2$ during the one-month period of aging and of maize cultivation amounted to $23{\sim}24%$ and $24{\sim}33%$, respectively, of the original $^{14}C$ activities. At harvest after one-month growing, the shoots and roots contained less than 0.1% and 1% of the originally applied $^{14}C$ activity, respectively, whereas the $^{14}C$ activity remaining in soil was $65{\sim}80%$ in both soils. Three degradation products with m/z 198(3-phenoxybenzaldehyde), m/z 214(3-phenoxybenzoic acid), and m/z 228(methyl 3-phenoxybenzoate) besides an unknown were identified from acetone extracts of both soils without and with maize plants after treatment of [$^{14}C$]acrinathrin, by autoradiography and GC-MS, and those with m/z 225(3-phenoxybenzaldehyde cyanohydrin) and m/z 198 (3-phenoxybenzaldehyde) from acetone extract of the Soil A treated with 50 ppm acrinathrin and grown with maize plants for 30 days were identified by mass spectrometry. These results suggested that the hydrolytic cleavage of the ester linkage adjacent to the $^{14}C$ with a cyano group, forming 3-phenoxybenzaldehyde cyanohydrin. The removal of hydrogen cyanide therefrom leads to the formation of 3-phenoxybenzaldehyde as one of the major products. The subsequent oxidation of the aldehyde to 3-phenoxybenzoic acid, followed by decarboxylation would evolve $^{14}CO_2$. Solvent extractability of the soils where maize plants were grown for 1 month and/or [$^{14}C$]acrinathrin was aged for 1 month was less than 31% of the original $^{14}C$ activity and over 95% of the total $^{14}C$ activity in soil extracts was distributed in the organic phase. Accordingly, acrinathrin turned out to be degraded rapidly in both soils and be bound to soil constituents as well, not being available to crops.

• Korean Journal of Environmental Agriculture
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• v.14 no.2
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• pp.202-212
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• 1995

In order to elucidate the degrading characteristics of the pyrethroid acaricide-insecticide, acrinathrin in two different types of soils, Soil A(pH, 5.8; organic matter, 3.4%; C.E.C., 115 mmol(+)/kg soil; texture, sandy loam) and Soil B(pH, 5.7; organic matter, 2.0%; C.E.C., 71 mmol(+)/kg soil; texture, sandy loam), residualities of the non-labeled compound under the field and laboratory conditions, extractability with organic solvents and formation of non-extractable bound residues, and degradabilities of [$^{14}C$]acrinathrin as a function of aging temperature and aging period were investigated. The half lives of acrinathrin in Soil A treated once and twice were about 18 and 22 days and in Soil B about 13 and 15 days, respectively, in the field, whereas, in the laboratory, those in Soil A and B were about 36 and 18 days, respectively, suggesting that the compound would be non-persistent in the environment. The amounts of $^{14}CO_2$ evolved from [$^{14}C$]acrinathrin in Soil A and B during the aging period of 24 weeks were 81 and 62%, respectively, of the originally applied $^{14}C$ activity, and those of the non-extractable soil-bound residues of [$^{14}C$]acrinathrin were about 70% of the total $^{14}C$ activity remaining in both soils, increasing gradually with the aging period. Degradation of [$^{14}C$]acrinathrin in both soils increased with the aging temperature. Three degradation products of m/z 198(3-phenoxy benzaldehyde), m/z 214(3-phenoxybenzoic acid), and m/z 228(methyl 3-phenoxybenzoate) as well as an unknown were detected by autoradiography of acetone extracts of both soils treated with [$^{14}C$]acrinathrin and aged for 15, 30, 60, 90, 120, and 150 days, respectively, and the degradation pattern of acrinathrin was identical in both soils. Acrinathrin in soil turned out to be degraded to 3-phenoxybenzaldehyde cyanohydrin by hydrolytic cleavage of the ester linkage adjacent to the $^{14}C$ with a cyano group, the removal of hydrogen cyanide therefrom led to the formation of 3-phenoxybenzaldehyde as one of the major products, and the subsequent oxidation of the aldehyde to 3-phenoxybenzoic acid, followed by decarboxylation would lead to the evolution of $^{14}CO_2$.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.121-130
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• 1985

The phenolic compounds identified from rye and wheat residues seemed to be p-coumaric, p-hydroxybenzoic, vanillic, ferulic, salicylic and syringic acids. Total phenol content of rye and wheat straw determined at heading stage was 0.1803% and 0.1702%, respectively. Total phenol content in straw was higher than that of root at all growth stages. The germination and growth of plants, such as Oryza sativa, Echinochloa creugalli, Cyperus serotinus, Portulaca oleacea, Amaranthus retroflexus, Digitaria saguinalis, and C. album were inhibited by treatments of authentic phenolic compounds as the concentrations increased. However, at the early stage, the germination and shoot growth of P. distinctus were markedly stimulated by them, and then the further growth of shoot and root was markedly inhibited by the prolonged treatment of phenolic compounds. The aqueous extracts from rye and wheat straw completely inhibited the germination of A. retroflexus and C. album at a high concentrations. The content of starch and protein in bulbs of P. distinctus was lower in the p-hydroxybenzoic acid treated plot, at $10^{-2}M$, than the untreated control.