• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.19-22
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• 2003

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We found that Drosaphiia FACT, a heterodimer of dSPT16 and dSSRPl, is associated with GAGA factor through its dSSRPl subunit, binds to a nucleosome and facilitates GAGA factor-directed chromatin remodeling. Immunostaining of polytene chromosomes revealed colocalization of GAGA factor and FACT in many specific loci. (omitted)

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.1
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• pp.130-137
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• 1990

Thermal shock stress intensity factor for an edge-cracked plate subjected to thermal shock is obtained from Bueckner's weight function method. It is shown that thermal shock stress intensity factor has maximum values with variation of time and crack length and that there is most dangerous crack length. By comparing thermal shock stress intensity factor with fracture toughness, the fracture time and critical temperature difference due to thermal shock are determined theoretically. Under constant thermal shock temperature difference, and increase of crack length is shown to increase fracture time. The theoretical fracture time is compared with experimental value measured by acoustic emission method with soda lime glass.

• Molecules and Cells
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• v.20 no.3
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• pp.364-370
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• 2005

We show that sulforaphane inhibits osteoclastogenesis in the presence of macrophage colony-stimulating factor (M-CSF) and receptor for activation of nuclear factor-${\kappa}B$ ligand (RANKL) in osteoclast (OC) precursors. Sulforaphane, an aliphatic isothiocyanate, is a known cancer chemo-preventative agent with anti-oxidative properties. Nuclear factor-${\kappa}B$ (NF-${\kappa}B$) is a critical transcription factor in RANKL-induced osteoclastogenesis, and electrophoretic mobility shift assays (EMSAs) and assay of NF-${\kappa}B$-mediated secreted alkaline phosphatase (SEAP) revealed that sulforaphane selectively inhibited NF-${\kappa}B$ activation induced by RANKL. Inhibition may involve interaction of sulforaphane with thiol groups, since it was prevented by reducing agents.

• Journal of Information Technology Applications and Management
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• v.9 no.4
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• pp.179-204
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• 2002

The purpose of this study is to empirically Investigate the critical success factors affecting the participation of B2B e-marketplace from a perspective of buyers and sellers. The research model presented in this study suggests that the success of B2B e-marketplace depends on the degree of participation of buyers and sellers. It is hypothesized that the participation of buyers and sellers is related with several contextual factors. The contextual factors included: (a) industry and market factor; (b) Internal environment factor; (c) product factor; (d) Inter-organizational factor; and (e) B2B e-marketplace system and strategy factor. An analysis of data from 31 buyer firms and 31 seller firms reveals that those five factors have a significant effect on the participation and success of B2B e-marketplace. The implications of the study are discussed and further research directions are proposed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.11
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• pp.1535-1539
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• 2000

A sonic nozzle is used as a reference flow meter in the area of gas flow rate measurement. The critical pressure ratio of sonic nozzle is an important factor in maintaining its operating condition. ISO9300 suggested the critical pressure ratio of sonic nozzle as a function of area ratio. In this study, 13 sonic nozzles were made by the design of ISC9300 with different half diffuser angles of 2。 to 8。 and throat diameters of 0.28 to 4.48 mm. The test results of half diffuser angles below 8。 ar quite similar to those of ISO9300. On the other hand, the critical pressure ratio for the nozzle of 8。 decreases by 5.5% in comparison with ISO9300. However, ISO9300 does not predict the critical pressure ratio at lower Reynolds numbers than 10(sup)5. Therefore, it is found that it is a better way for the flow of low Reynolds number to express the critical pressure ratio of sonic nozzle as a function of Reynolds number than area ratios. A correlation equation of critical pressure is introduced with uncertainty $\pm$3.2 % at 95% confidence level.

• Journal of Korean society of Dental Hygiene
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• v.19 no.6
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• pp.1037-1046
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• 2019

Objectives: This study was conducted to test the critical thinking tendency, clinical practice satisfaction, and clinical performance, and analyze the factors impacting clinical practice satisfaction and clinical performance of dental hygiene students. Methods: The study conducted a written survey during the period between 30 July ~20 August 2019, among 3rd and 4th year dental hygiene students from Seoul, Gyung-gi region, who had taken clinical practice courses. Using SPSS 22.0 program (IBM SPSS statistics, New York, USA), the study analyzed the final 174 cases. Results: Dental hygiene students scored 3.33±0.43, 3.48±0.83, and 3.30±0.58 for critical thinking, clinical practice satisfaction, and clinical performance, respectively. The clinical thinking score was higher when their study performance was good (p<0.001), clinical practice satisfaction was higher when their major satisfaction was satisfactory (p<0.001), higher when their relationship with their clinical practice partner was good, and the clinical performance was better when the students' major satisfaction was good (p<0.001). The study performance yielded better results when their study achievement was better (p<0.05). Clinical practice satisfaction (r=0.156) and clinical performance (r=0.393) showed a positive correlation with critical thinking, and clinical performance had a positive correlation with clinical practice satisfaction. The impact factor for clinical practice satisfaction appeared to be clinical performance (p<0.05) and major satisfaction (p<0.001), and the factors impacting the clinical performance were among the sub-causes of critical thinking (p<0.05), intellectual fairness (p<0.05), watchfulness (p<0.05), clinical practice satisfaction (p<0.05), and major satisfaction (p<0.01). Conclusions: Results of the study showed that for dental hygiene students, critical thinking, clinical practice satisfaction, clinical performance, and major satisfaction were the impact factors affecting the students' clinical performance. Therefore, the study recommends that development of educational programs and operation of a field-based curriculum is necessary to improve critical thinking, clinical practice satisfaction, and major satisfaction.

• Tuberculosis and Respiratory Diseases
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• v.70 no.2
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• pp.113-124
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• 2011

Background: Macrophages are one of the most important inflammatory cells in innate immunity. PU.1 is a macrophage-specific transcription factor. Ubiquitins are the ultimate regulator of eukaryotic transcription. The ubiquitination process for PU.1 is unknown. This study investigated the lipopolysaccharide (LPS)-induced activation of PU.1 and its relation to ubiquitins in the macrophages. Methods: Raw264.7 cells, the primary cultured alveolar, pulmonary, and bone marrow derived macrophages were used. The Raw264.7 cells were treated with MG-132, $NH_4Cl$, lactacytin and LPS. Nitric oxide and prostaglandin D2 and E2 were measured. Immunoprecipitation and Western blots were used to check ubiquitination of PU.1. Results: The PU.1 ubiquitination increased after LPS ($1{\mu}g$/mL) treatment for 4 hours on Raw264.7 cells. The ubiquitination of PU.1 by LPS was increased by MG-132 or $NH_4Cl$ pretreatment. Two hours of LPS treatment on macrophages, PU.1 activation was not induced nor increased with the inhibition of proteasomes and/or lysosomes. The ubiquitination of PU.1 was increased in LPS-treated Raw264.7 cells at 12- and at 24 hours. LPS-treated cells increased nitric oxide production, which was diminished by MG-132 or $NH_4Cl$. LPS increased the production of $PGE_2$ in the alveolar and peritoneal macrophages of wild type mice; however, $PGE_2$ was blocked or diminished in Rac2 null mice. Pretreatment of lactacystin increased $PGE_2$, however it decreased the $PGD_2$ level in the macrophages derived from the bone marrow of B57/BL6 mice. Conclusion: LPS treatment in the macrophages ubiquitinates PU.1. Ubiquitination of PU.1 may be involved in synthesis of nitric oxide and prostaglandins.

• Tuberculosis and Respiratory Diseases
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• v.75 no.3
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• pp.95-103
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• 2013

Background: Small cell lung cancer (SCLC) transformation during epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) treatment in lung cancer has been suggested as one of possible resistance mechanisms. Methods: We evaluated whether SCLC transformation or neuroendocrine (NE) differentiation can be found in the cell line model. In addition, we also investigated its effect on responses to conventional chemotherapeutic drugs of the SCLC treatment. Results: Resistant cell lines to various kinds of EGFR-TKIs such as gefitinib, erlotinib, CL-387,785 and ZD6474 with A549, PC-9 and HCC827 lung adenocarcinoma cell lines were established. Among them, two resistant cell lines, A549/GR (resistant to gefitinib) and PC-9/ZDR (resistant to ZD6474) showed increased expressions of CD56 while increased synaptophysin, Rb, p16 and poly(ADP-ribose) polymerase were found only in A549/GR in western blotting, suggesting that NE differentiation occurred in A549/GR. A549/GR cells were more sensitive to etoposide and cisplatin, chemotherapeutic drugs for SCLC, compared to parental cells. Treatment with cAMP and IBMX induced synaptophysin and chromogranin A expression in A549 cells, which also made them more sensitive to etoposide and cisplatin than parental cells. Furthermore, we found a tissue sample from a patient which showed increased expressions of CD56 and synaptophysin after development of resistance to erlotinib. Conclusion: NE differentiation can occur during acquisition of resistance to EGFR-TKI, leading to increased chemosensitivity.

• Tuberculosis and Respiratory Diseases
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• v.85 no.2
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• pp.155-164
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• 2022

Background: The remarkable efficacy of osimertinib in non-small cell lung cancer (NSCLC) with acquired T790M mutation has been widely documented in clinical trials and real-world practice. However, some patients show primary resistance to this drug. Even patients who initially show a favorable response have inconsistent clinical outcomes later. Therefore, the aim of this study was to identify additional clinical predictive factors for osimertinib efficacy. Methods: A prospective cohort of patients with acquired T790M positive stage IV lung adenocarcinoma treated with osimertinib salvage therapy in Hallym University Medical Center were analyzed. Results: Sixty-one eligible patients were analyzed, including 38 (62%) women and 39 (64%) who never smoked. Their mean age was 63.3 years. The median follow-up after treatment with epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) was 36.0 months (interquartile range, 24.7-50.2 months). The majority (n=45, 74%) of patients were deceased. Based on univariate analysis, low baseline neutrophil-to-lymphocyte ratios (NLR), age ≥50 years, never-smoking history, stage IVA at osimertinib initiation, and prolonged response to previous TKIs (≥10 months) were associated with a significantly longer progression-free survival (PFS). Multivariate analysis showed that never-smoking status (hazard ratio [HR], 0.54; 95% confidence interval [CI], 0.30-0.98; p=0.041) and a baseline NLR less than or equal to 3.5 (HR, 0.23; 95% CI, 0.12-0.45; p<0.001) were independently associated with a prolonged PFS with osimertinib. Conclusion: Smoking history and high NLR were independent negative predictors of osimertinib PFS in patients with advanced NSCLC developing EGFR T790M resistance after the initial EGFR-TKI treatment.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.5
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• pp.531-539
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• 1991

We develop an analytical model of the static and the dynamic characteristics of amorphous silicon thin film transistors (a-Si TFTs) in order to incorporate into a widely used circuit simulator such as SPICE. The critical parameters considered in our analytical model of a-Si TFT are the power factor (XN) of saturation source-drain current and the effective channel length (L') at saturation region. The power factor, XN must not always obey so-called