• Journal of Marine Life Science
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• v.1 no.1
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• pp.25-30
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• 2016

We investigated the possibility of a bio-monitoring system for detecting hypoxic water in coastal area using shell valve movements of Pacific oyster (Crassostrea gigas), which showed most aquaculture production in Korea, with Hall element sensor. In filtrating water to confirm shell valve movement (SVM) under normal condition, it showed spikes which mean a relatively fast closing condition after opened condition of average 5~12 mm, and then the SVM showed back to opening condition slower than closing speed SVM numbers during light period were similar to that of dark period (p<0.05). When dissolved oxygen (DO) concentration was reduced from 7 mg l^-1 to 3 mg l^-1, SVM numbers were increasing with decreasing of DO, and showed abnormality SVMs as compare with normal condition. Moreover, in the condition of 2 mg l^-1, Distance between light and left shell showed gradually decreased, and then we could not detected SVMs due to closed condition. Thus, if we quickly detect abnormal environmental variations as hypoxia water using bio-monitoring of SVM, it may be contribute to increased productivity by dramatically reducing damages in aquaculture.

• Proceedings of the Zoological Society Korea Conference
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• 2000.04a
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• pp.83.1-83
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• 2000

No Abstract, See Full Text

• Proceedings of the Malacological Society of Korea Conference
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• 1999.11a
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• pp.14-15
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• 1999

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.385.2-386
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• 1998

• The Korean Journal of Malacology
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• v.12 no.2
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• pp.91-97
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• 1996

바윗굴의 산란유발 및 종묘생산을 위한 생물학적 기초자료를 얻고자 자극방법별 효과와 난발생 및 유생사육에 미치는 수온의 영향에 관하여 실험한 결과, 자극방법별 산란유발은 정자현탁액 첨가구에서 가장 많은 산란량과 높은 수정률을 나타냈고, 난발생 및 유생사육의 각 단계에 이르기까지의 수온(T, $^{\circ}C$)에 따른 발생속도(h, 시간)는 수온이 높을 수록 빨랐으며, 그 관계식은 다음과 같다. 담륜자기 :1/h= 0.0069T - 0.0950(r=0.9447)D형 유생 :1/h= 0.0006T - 0.0045(r=0.9288)초기 각정기 유생:1/h= 0.0002T - 0.0019(r=0.9358)후기 각정기 유생:1/h= 0.0002T - 0.0022(r=0.9868)부착기 유생:1/h= 0.0001T - 0.0013(r=0.9897)또한 바윗굴의 수온과 난발생 속도와의 관계에서 추정된 난발생의 생물학적 영도는 평균 10.96$^{\circ}C$였으며, 수온별 유생사육시 바윗굴의 생존율은 24$^{\circ}C$에서 6.8%로 가장 좋았다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.702-707
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• 2013

Sleep is an essential biological process of which the underlying regulatory mechanisms involve numerous anatomical structures and biochemical substances that can be compromised by stress and the immune system. Gamma aminobutyric acid (GABA) is the main inhibitory neurotransmitter of the central nervous system (CNS). It is well established that activation of $GABA_A$ receptors promotes sleep. L. brevis BJ20 fermentation of sea tangle and oysters resulted in stress reduction and sleep inducing effects. This is the first study to report that GABA has the ability to induce sleep related hormones in mice; therefore, it has potential use as a natural sleep aid. These results suggested that sea tangle and oysters fermented by L. brevis BJ20 can be used as potential agents for stress reduction and sleep promotion.

• The Korean Journal of Malacology
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• v.14 no.2
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• pp.103-111
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• 1998

참굴에 다환성방향족탄화수소(PAHs)의 축적성과 이 물질들이 미치는 독성을 평가하기 위해 해양환경에서 빈번히 검출되는 PAHs 중의 하나인 fluoranthene을 사용한 모델연구를 수행하였다. Fluoranthene을 0.01-1ppm의 농도로 2주간 참굴에 노출시켰을 때 참굴 조직내의 fluoranthene의 농도는 노출기간 및 노출농도에 비례하여 최고 40-70배까지 증가하였다. 소화맹낭과 폐각근의 지질 과산화물가는 노출기간 및 노출농도에 관련하여 현저히 증가하였으며 수용성단백질의 함량은 노출기간이 길어짐에 따라 서서히 감소하였다. 그러나 glycogen 함량, nucleoside/nucleotide, DNA 및 RNA 함량은 변화하지 않았다. 이 결과로 fluoranthene은 굴의 조직내로 원활하게 축적함을 확인하였다. 또한 과산화지질의 함량은 fluoranthene에의 오염에 대해 신속히 반응하는 지표의 하나로 추정되며 수용성단백질의 감소도 비록 시간적으로 다소 둔감하기는 하나 또 다른 지표가 될 수 있을 것으로 사료된다.

• The Korean Journal of Malacology
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• v.14 no.2
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• pp.113-120
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• 1998

참굴에 다환성방향족탄화수소(PAHs)의 축적성과 이 물질들이 미치는 독성을 평가하기 위해 해양환경에서 빈번히 검출되는 PAHs 중의 하나인 anthracene을 사용한 모델연구를 수행하였다. Anthracene을 0.01-1ppm의 농도로 2주간 참굴에 노출시켰을 때 참굴 조직내의 anthracene의 농도는 노출기간 및 노출강도에 비례하여 최고 25-85배까지 증가하였다. 소화맹낭과 폐각근의 지질 과산화물가는 노출기간 및 노출농도에 관련하여 현저히 증가하였으나 수용성단백질, glycogen, nucleoside/nucleotide 및 핵산의 함량은 변화하지 않았다. 이 결과로 anthracene은 굴의 조직내로 원할하게 축적됨을 확인하였으며 과산화지질의 함량은 anthracene에의 오염에 대한 예민한 생화학적 지표가 될 수 있을 것으로 사료된다.

• Journal of fish pathology
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• v.14 no.2
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• pp.103-105
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• 2001

The effect of opsonization of zymosan with serum on the repiratory burst of haemocytes isolated from the Pacific oyster Crassostrea gigas was investigated. The reactive oxygen species (ROS) produced by the respiratory burst of haemocytes in response to the opsonized or unopsonized zymosan were measured using chemiluminescence (CL). The CL values were increased according to the increment of haemocyte number. The degree of CL amplification by increase of haemocytes from $0.1{\times}10^6$ to $0.5{\times}10^6$ cells/ml was 3-5 times, but comparatively low amplification was elicited by increase of haemocytes from $0.5{\times}10^6$ to $1{\times}10^6$ cells/ml. Chemiluminescences produced by the haemocytes in response to the zymosan opsonized with oyster serum were considerably higher than the CL produced by the haemocytes in response to the unopsonized zymosan.

• Korean Journal of Pharmacognosy
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• v.4 no.1
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• pp.9-17
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• 1973

A score and more kinds of molluscan shells have been used as drugs in oriental medicine. The Korean Pharmacopoeia (K.P.) contains a monograph on oyster shell "Ostreae Testa" as an official drug. A huge deposit of the fossil shell Crassostrea gravitesta eoilensis has been recently excavated in the region of Wolsung, Kyong-Sang-Buk-Do. This study was conducted to determine the applicability of the fossil shell as a substiute for Ostreae Testa, K.P. The fossil shell not only met the K.P. requirements, but also exhibited an appropriate antacid activity. Amino acid survivals were also determined quantitatively with amino acid autoanalyzer.