• Title/Summary/Keyword: Cowpea chlorotic mottle virus

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Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR (Nested PCR을 이용한 Cowpea chlorotic mottle virus 정밀 진단 시스템 개발)

  • Min, Byung-Dae;Kim, Young-Suk;Lee, Siwon;Lee, Su-Heon
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.335-339
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    • 2014
  • Cowper chlorotic mottle virus (CCMV) is the 'controlled' quarantine virus as plant pathogenic virus that are classed as group VI (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.