• Korean Journal of Soil Science and Fertilizer
• /
• v.26 no.4
• /
• pp.236-240
• /
• 1993

This study was conducted to investigate the chemical properties of soils under plastic house cultivation in northern Gyeonggi province(Yangju gun and Paju gun) in 1992. The results are summarized as follows: 1. Distributions of soil texture were in the order of sand loam(65%), silt loam(25%) and loam(5%). 2. Content of soil nutrient varied with difference with soil management practices and years of cultivation and chemical properties of soils. 3. Soil pH, Ca/Mg ratio and Mg/K ratio tended to be low, while other parameters high. 4. The average application rates of poultry dropping, cow dung and piggery waste were 6.0, 4.5, and 2.0ton/10a, respectively.

• Journal of Embryo Transfer
• /
• v.30 no.4
• /
• pp.327-333
• /
• 2015

The objectives of the present study were to select an effective basic medium including its hormone and protein supplementation for IVM of oocytes of indigenous zebu cows. The ovaries of cows were collected from slaughter house and the follicular fluid was aspirated from 2 to 8 mm diameter follicles. The COCs with more than 3 cumulus cell layers and homogenous cytoplasm were selected for maturation. The oocytes were matured in media for 24 hrs at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The maturation of oocytes was evaluated by examining the presence of first polar body under microscope. An efficient basic medium was determined after culturing COCs in either TCM 199 or SOF medium in Experiment 1. An efficient hormone supplementation was determined after culturing COCs in either FSH or gonadotrophin supplemented TCM 199 in Experiment 2. An efficient protein supplementation was determined after culturing COCs in either FBS or Oestrous cow serum (OCS) supplemented TCM 199 in Experiment 3. The oocyte recovery rate per ovary was 3.35. The overall rate of IVM was 74.6%. The maturation rate was $75.5{\pm}3.9$ and $62.2{\pm}20.2%$ in TCM and SOF medium, respectively (P>0.05). The maturation rate of oocytes was significantly higher ($76.6{\pm}13.2%$) in FSH supplemented medium than gonadotrphin supplemented counterpart ($69.7{\pm}10.8%$) (P<0.05). The maturation rates of oocytes were $81.7{\pm}12.9$ and $85.7{\pm}12.7%$ in medium supplemented with FBS and OCS, respectively (P>0.05). In conclusions, both TCM 199 and SOF supplemented with either FBS or OCS, and FSH may be used as medium for IVM of indigenous zebu oocytes in Bangladesh.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.2
• /
• pp.230-238
• /
• 2011

Effects of cooling and supplemental recombinant bovine somatotropin (rbST) on hemato-biochemical characteristics were studied at different stages of lactation of crossbred Holstein Friesian cows in a tropical environment. Ten primiparous cows were divided into two groups of five animals each. The first group was housed as the non-cooled animals in an open-sided barn with a tiled roof in a normal shaded house (NS), while the second group was housed as cooled cows in an open-sided barn with a tiled roof under misty fan cooling (MFC). Three injections with rbST (500 mg per dose) at each stage of lactation (early, mid and late lactation) significantly increased total milk yield as compared with pretreatment in both cooled and non-cooled cows. Milk fat was significantly increased, while total solids, solid not fat, milk protein and lactose were not affected by the rbST treatment. Hematological parameters, plasma proteins, albumin, glucose, triglyceride, cholesterol, creatinine, alkaline phosphatase (ALP), plasma inorganic phosphate and the activities of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected by supplemental rbST in cooled and non-cooled cows. Supplementation of rbST caused a significant decrease in plasma urea concentration, while plasma FFA concentrations significantly increased in both cooled and non-cooled cows. The results of the present study suggest that exogenous rbST is efficacious in increasing milk yield without adverse effects on lactating crossbred Holstein cows in a tropical environment.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.4
• /
• pp.295-303
• /
• 1997

To investigate the transformation characteristics of nitrogen and carbon from cow manure compost amended in soil under different moisture conditions, dynamics of nitrogen and carbon were determined periodically for 15 weeks of aerobic incubation at room temperature during July${\sim}$November, 1996. Cow manure compost matured with mixing saw dust was amended with the 4 ratios (0, 2, 4, 6%(wt/wt)) in Ap horizon soil, which collected from green house in Yesan, Chungnam. Moisture was controlled with 0.2, 0.3, 0.4, and 0.5 of mass water conte nt (${\theta}$m) to air dried soil, and water loss was compensated at every sampling. During incubation, soil pH was decreased continuously, that was caused by hydrogen generated from nitrification of ammonium nitrogen. And pH became higher with inclining cow manure compost amendment and water treatment, that meaned the increase of mineralization of organic-N to $NH_4\;^+-N$. Total nitrogen was reduced with increasing water content, but total carbon showed the contrast tendency with that of nitrogen. Therefore, C/N ratio slightly decreased in the low water condition (${\theta}$m 0.2) during incubation, but increased continuously in high water condition over ${\theta}$m 0.4. As a result, it was assumed that soil fertility is able to be reduced in the high water content over available water content. Nitrate transformation rate increased lasting in the low water content less than ${\theta}$m 0.3. Itdropped significantly in the first $2{\sim}3$ weeks of incubation over ${\theta}$m 0.4. In particular, nitrate was not detected in ${\theta}$m 0.5 of water content after the first $2{\sim}3$ weeks. In contrast, ammonium transformation was inclined with increasing water treatment. Nitrogen mineralization rate, which calculated with percentage ratio of (the sum of ex.$NH_4\;^+-N$ and $NO_3\;^--N$)/total nitrogen, was continuously increased in the low water content of ${\theta}$m 0.2 and 0.3. But it saw the different patterns in high water content over ${\theta}$m 0.4 that was drastically declined in the initial stage and then gradually inclined . From the above results, nitrogen transformation patterns differentiated decisively in water content between ${\theta}$m 0.3 and 0.4 in soil. Thus, it is very important for the maintain of suitable soil water content to enhance fertility of soil amended with manure compost. However, excess treatment of manure compost might enhance the possibility of contamination of small watershed and ground water around agricultural area.

• Journal of Life Science
• /
• v.26 no.5
• /
• pp.592-602
• /
• 2016

This study investigated the muscular dystrophin levels in freely moving Australian cattle mainly fed grass, freely moving Korean cattle fed mainly a grain diet, and Korean cattle fed a grain diet but housed in a relatively limited space of a cow house. The total skeletal muscle specimens of 244 cattle were collected and immediately fixed in 10% neutral formalin. The same area was biopsied from the cattle in both countries. The findings showed that fatty infiltration is highly correlated with membrane-associated protein degradation in skeletal muscle, and that among several membrane-associated proteins, dystrophin showed the most significant reduction in expression in the cattle with fatty infiltration. Similarly, CD36 was more highly expressed in the cattle with fatty infiltration of skeletal muscle. Various breeding factors, such as oxidative stress; the presence of oxidized lipids in the diet; and environmental factors such as exercise, temperature and amount of time spent, may have critical effects on the degradation of normal cytoskeleton proteins, which are required for maintaining normal skeletal muscle architecture. Among the sarcolemma membrane-associated proteins, dystrophin is the most sensitive membrane protein that is involved muscular dystrophy and muscular degeneration. Thus, the present findings may be useful for studies on muscular dystrophy in humans or the pathogenesis of muscular diseases in animal models.

• The Korean Journal of Soil Zoology
• /
• v.5 no.2
• /
• pp.125-131
• /
• 2000

Disposing technique for dairy cattle manure using earthworms and the possibility of utilizing earthworms in poultry feed were investigated. In addition, chemical properties of manure and cast of earthworms were investigated. Temperature in the vinyl-house for earthworm nursery was able to be maintained above 0$\^{C}$ in winter and 25-28$\^{C}$ in summer. In closed-covering method, the number of escaped earthworms was the highest among three covering methods. The time was longer after manure excretion, the numbers of escaped earthworm were higher. There were some incidences of mortality of earthworm in closed-covering site. The amount of manure ingested by earthworm was increased in order of non-covering, closed-covering and gap-covering method. Fresh manure was more ingested than old one by earthworm. Earthworms reduced manure about 50% on the dry weight base and reduced total amounts of N, P$_2$O$\_$5/ and K$_2$O of manure by 41, 50 and 60%, respectively. Earthworm could be utilized by adult poultry (>60 g) for feeding. However, adverse effect of live earthworm was observed with young poultry (<450 g).

• Korean Journal of Veterinary Service
• /
• v.31 no.3
• /
• pp.375-384
• /
• 2008

This study was performed to establish the discrimination of the corpus albicans that were formed after normal parturition and abortion in Holstein, The corpus albicans were obtained from 504 cows and 506 heifers slaughtered in the Agricultural Cooperative's Livestock Products Joint Market of Seoul City from March 2007 to October 2007. The difference in a gross examination (morphology, fibrogenesis, deposit state, color, and size) among the fixtures that exist in their ovaries was investigated. All of the cows had corpus albicans in their ovaries: 423 cows (83.9%) had less than three corpus albicans, 81 (16.1%) had more than four. In the heifers, however, 463 (91.05%) had no corpus albicans, 39 (7.7%) had one, and four (0.8%) had more than two. In the types of corpus albicans 73.2% of the cows had oval-type, 14.4% had circular-shaped, 12.4% had irregular-type. In the aborted heifers, however, oval-type appeared in 88.6% and circular-shaped appeared in 11.4%, and no irregular-type was found. There was no significant difference in the fibrogenesis and size of corpus albicans between cows and aborted heifers. However, slight difference in the color of the corpus albicans was observed between two types. In conclusion, it is not easy to discriminate by gross examination of ovary between cows and aborted heifers in the slaughter house.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.10 no.6
• /
• pp.482-493
• /
• 2005

Biomass is originally photosynthesized from inorgainic compounds such as $CO_2$, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellullosic or chitinoic materials to useful compounds. The biomass containing agricultural waste, unutilized wood and other garbage is expected to utilize as feed, food and fuel by microbial degradation and other metabolic functions. In this study we isolated several anaerobic, cellulolytic and chitinolytic bacteria from rumen fluid, compost and soil to study their related enzymes and genes. The anaerobic and cellulolytic bacteria, Clostridium thermocellum, Clostridium stercorarium, and Clostridium josui, were isolated from compost and the chitinolytic Clostridium paraputrificum from beach soil and Ruminococcus albus was isolated from cow rumen. After isolation, novel cellulase and xylanase genes from these anaerobes were cloned and expressed in Escherichia coli. The properties of the cloned enzymes showed that some of them were the components of the enzyme (cellulase) complex, i.e., cellulosome, which is known to form complexes by binding cohesin domains on the cellulase integrating protein (Cip: or core protein) and dockerin domains on the enzymes. Several dockerin and cohesin polypeptides were independently produced by E. coli and their binding properties were specified with BIAcore by measuring surface plasmon resonance. Three pairs of cohesin-dockerin with differing binding specificities were selected. Two of their genes encoding their respective cohesin polypeptides were combined to one gene and expressed in E. coli as a chimeric core protein, on which two dockerin-dehydrogenase chimeras, the dockerin-formaldehyde dehydrogenase and the dockerin-NADH dehydrogenase are planning to bind for catalyzing $CO_2$ reduction to formic acid by feeding NADH. This reaction may represent a novel strategy for the reduction of the green house gases. Enzymes from the anaerobes were also expressed in tobacco and rice plants. The activity of a xylanase from C. stercorarium was detected in leaves, stems, and rice grain under the control of CaMV35S promoter. The digestibility of transgenic rice leaves in goat rumen was slightly accelerated. C. paraputrificum was found to solubilize shrimp shells and chitin to generate hydrogen gas. Hydrogen productivity (1.7 mol $H_2/mol$ glucos) of the organism was improved up to 1.8 times by additional expression of the own hydrogenase gene in C. paraputrficum using a modified vector of Clostridiu, perfringens. The hydrygen producing microflora from soil, garbage and dried pelletted garbage, known as refuse derived fuel(RDF), were also found to be effective in converting biomass waste to hydrogen gas.

• Korean Journal of Veterinary Research
• /
• v.36 no.4
• /
• pp.919-927
• /
• 1996

In the last few years, methods for in vitro culture of early embryo stages from oocytes matured and fertilized in vitro using suitable cell culture systems have been established. But the factors affecting pregnancy rates following transfer of bovine embryos produced in vitro were not evaluated enough. So this study was performed to investigate the effects of quality and stage of embryos, parity and Corpus Luteum quality of recipients on pregnancy rates following non-surgical transfer of bovine embryos produced in vitro. Oocytes aspirated from small antral follicles of ovaries obtained at a local slaughter house were matured, fertilized with frozen-thawed semen and co-cultured for 6-7 days by utilizing co-culture system with bovine oviduct epithelial cell in vitro. After co-culture, embryos were transfered to recipients on day 7 (estrus=day 0). Recipients were monitored by ultrasonic scanning method or observation for estrus and rectal palpation after 50 days from transfer. The results of this study are follows. 1. Of the 70 recipients, 70%(49 of 70) had not showed estrus sign between day 0 and day 50, but 22.9%(16 of 70) was diagnosed not pregnant. Therefore the overall pregnancy rate of this study was 47.1%(33 of 70). 2. The pregnancy rate of recipients transfered with excellent(66.7%) and good(54.5%) embryos were higher than that of recipients transfered with fair embryos(15.8%) (p<0.05). 3. The pregnancy rate of recipients transfered with morula, compacted morula, blastocyst and expanded blastocysts were 46.2, 55.0, 62.5 and 50.0%, respectively. 4. The pregnancy rates of recipients transfered to heifer and cow were 54.5 and 55.2%, respectively. 5. The pregnancy rates of recipients with CL score I, II(66.7, 63.6%) were higher than those of recipients with CL score III (10%), (p<0.05). Success of transfer of embryos produced in vitro depends on many variables. The important factors identified in this study were the quality of embryos and the CL score of recipient animals after non-surgical transfer of embryos matured, fertilized and cultured in vitro.

• Korean Journal of Veterinary Research
• /
• v.22 no.2
• /
• pp.91-98
• /
• 1982

This study was conducted to determine the interrelationship between the weight of various visceral organs and the live weight of Korean native cattle with particular regard to female ones. One hundred and eighty-five of 2 to 6 years and one hundred and fifty-six Korean native cow of over 7 years of age were examined at the Busan slaughter house during the lest quarter of 1979. The results obtained are summarized as follows: 1. The average live weight of 72 years old cows examined was 297.09kg, while these weight of visceral organs such as liver, lung, heart, left kidney, right kidney and spleen being 4301.09, 2165.22, 1355.43, 346.74, 350 and 991.30g, respectively. The length, width and thickness of the spleen were 49.0, 12.92 and 2.95cm, respectively. 2. The average live weight of 3 years old cows examined was 306.56kg, while these weight of visceral organs such as liver, lung, heart, left kidney, right kidney and spleen being 4099.07, 2144.44, 1383.33, 326.85, 325.93 and 964.82g, respectively. The length, width and thickness of the spleen were 43.3, 12.37 and 2.80cm, respectively. 3. The average live weight of 4 years old cows examined was 314.67kg, while these weight of visceral organs such as liver, lung, heart, left kidney, right kidney and spleen being 4186.67, 2292.50, 1392.50, 346.67, 343.33 and 932.50g, respectively. The length, width and thickness of the spleen were 47.01, 12.55 and 3.08cm, respectively. 4. The average live weight of 5 years old cows examined was 328.90kg, while these weight of visceral organs such as liver, lung, hearts left kidney, right kidney and spleen being 4465.32, 2394.35, 1493.55, 370.16, 335.97 and 997.58g, respectively, The length, width and thickness of the spleen were 49.17, 12.76 and 3.16cm, respectively. 5. The average live weight of 6 years old cows examined was 334.97kg, while these weight of visceral organs such as liver, lung, heart, left kidney, right kidney and spleen being 4585.29, 2552.79, 1555.89, 364.70, 373.24 and 985.29g, respectively. The length, width and thickness of the spleen were 48.27, 12.66 and 2.78cm, respectively. 6. The average live weight of 156 cows (over 7 years of age) examined was 344.91kg, while these weight of visceral organs such as liver, lung, heart, left kidney, right kidney and spleen being 4730.77, 2651.44, 1554.39, 407.37, 409.42 and 1101.06g, respectively. The length, width and thickness of the spleen were 51.04, 13.21 and 3.08cm, respectively. 7. Total average live weight of 341 cows (from 2 to 6 and over 7 years of age) examined was 332.44kg, while these weight of visceral organs being 4550.95, 2497.13, 1500.76, 380.18, 382.27 and 1047.98g, respectively. The length, width and thickness of the spleen were 52.01, 12.96 and 3.02cm, respectively. 8. The correlation coefficients between the live weight of 341 cows and their visceral organs were found to be highly significant (P<0.01), except fort for the length of spleen