• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.630-635
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• 1996

For a survey of the occurrence of aflatoxin M$_{1}$ (AFM$_{1}$) in domestic cow's milk, we developed an enzyme-linked immunosorbent assay (ELISA) system, and quantitated the toxin in cow's milk. In order to produce specific antibodies AFM, conjugated to bovine serum albumin (AFM$_{1}$-BSA) and Freund's adjuvant were immunized subcutaneously to rabbits. By use of the antiserum showing the highest titer and AFB$_{1}$-HRP conjugate, we established a competitive direct ELISA (cdELISA) for AFM$_{1}$, whose detection limit was 0.003 ppb. The cross-reactivities of the antiserum against aflatoxin M$_{1}$ M$_{2}$, B$_{1}$, B$_{2}$, G$_{1}$, G$_{2}$, B$_{2a}$, and G$_{2a}$, were 100, 29.9, 25.0, 2.7, 13.0, 0.65, 0, and 0%, respectively. When the cdELISA was applied to the cow's milk spiked with AFM$_{1}$ and followed by cleanup with C$_{18}$ cartridge, the mean recovery of the assay was 104% (mean of CV, 6.4%) in the final concentration of 0.01-1 ppb (10-1, 000 ppt). When cow's milk samples gathered from markets and farms were assayed by the cdELISA, the mean concentration and SD of AFM$_{1}$ was 80.4 $\pm$ 55.0 ppt (n=64; range, 5.6-280 ppt).

• Parasites, Hosts and Diseases
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• v.46 no.3
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• pp.139-143
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• 2008

Ingestion of raw animal liver has been suggested as a possible mode of infection of human toxocariasis, We evaluated the relationship between toxocariasis and the ingestion of raw meat in patients with eosinophilia of unknown etiology. The study population consisted of 120 patients presenting with peripheral blood eosinophilia (> $500\;cells/{\mu}l$ or > 10% of the white blood cell count). They were divided into 2 groups: 104 seropositive patients based on a Toxocara excretory-secretory IgG ELISA and 16 seronegative patients. While 25.0% of seronegative patients had a recent history of eating raw cow liver, 87.5% of seropositive patients had this history. Multivariate statistical analysis showed that a recent history of eating raw cow liver was related to an increased risk of toxocariasis. Collectively, it is proposed that raw cow liver is a significant infection source of toxocariasis in the patients with eosinophilia of unknown etiology.

• Journal of Animal Environmental Science
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• v.7 no.2
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• pp.127-136
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• 2001

This experiment was carried out to investigate the effect of supplementing phosphorus to Korean cow manure on the growth and cast production of earthworm(Eisenia Foetida), and to estimate the optimal phosphorus level for the growth and cast production of earthworm and ratios of available phosphorus and calcium content of cast in optimal phosphorus levels. Phosphorus supplementing ratios to Korean native cow were 0, 1%, 2%, 4% and 8A%, respectively. The volume of raising box was 3375cm$^3$(15$\times$15$\times$15cm), and 500g of cow manure filled up to 10cm layer. Raising density was 90㎤ per worm during the experimental period(60 days). The maximum fresh weight and cast production of earthworm were obtained at 2~4% and 1% levels of phosphorus supplementation to Korea native cow manure. The total phosphorus and available phosphorus contents of earthworm tissues were not significant among in all treatments. There was a positive significantly differences between total phosphorus and available phosphorus of cast and residual matters. Available phosphorus content of cast were ranged from 9.3mg/g to 17.3mg/g at 1~4% levels of phosphorus supplementation. Ratios of available phosphorus and calcium contents of cast were 1.94~3.15:1 and 0.87~1.33:1 at 2~4% and 0~1% levels of phosphorus supplementation.

• Korean Journal of Veterinary Service
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• v.46 no.1
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• pp.81-85
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• 2023

This case report is about hemolymph nodes found in a dairy cow whose function is still not fully elucidated. A 4-month Holstein cow presented severe respiratory symptoms and hematochezia for a while with respiratory acidosis and metabolic alkalosis. Coccidiosis was diagnosed and treated immediately, but the cow died from respiratory acidosis and metabolic alkalosis. At necropsy, no abnormal appearance in thoracic and peritoneal organs was observed, but hemolymph nodes were observed being multifocally stuck on omasum serosa and the subcutaneous fascia of abdominal region, and the larger dark red lymph nodes were found along the omasum great curvature. Microscopically, lymphoid depletion and lymphadenitis in the lymph nodes were examined to point systemic infection, and in the hemolymph node, multifocally demarcated pale lesions with macrophage infiltration and fibrin deposition nearby subcapsular sinus. In subcapsular sinus of the hemolymph node, rod to linear gram-negative bacteria were found. Through this study, we might conclude that the hemolymph node is involved in pathogen phagocytosis.

• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.227-230
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• 2001

To know and apply the factors affecting calf's gender may increase our competitiveness of Hanwoo industry. Thus, this study was carried out to analyse the effect of expected factors on calf's gender and to make an alternative idea for desired calf's gender. Gender of calves born by AI was not affected by AI year and cow parity, but significantly affected by sire and farm. Therefore, to obtain calves' gender that we want, both selecting optimum sire for AI and providing optimum feeding environment for cow should be required.

• Journal of Institute of Control, Robotics and Systems
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• v.13 no.6
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• pp.560-567
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• 2007

The objective of this research is to optimize the designing parameters of the parallel manipulator with large orientation workspace at the boundary position of the constant orientation workspace (COW). The method uses a simple genetic algorithm(SGA) while considering three different kinematic performance indices: COW and the global conditioning index(GCI) to evaluate the mechanism's dexterity for translational motion of an end-effector, and orientation workspace of two angle of Euler angles to obtain the large rotation angle of an end-effector at the boundary position of COW. Total fifteen cases divided according to the combination of the sphere radius of COW and rotation angle of orientation workspace are studied, and to decide the best model in the total optimized cases, the fuzzy inference system is used for each case's results. An optimized model is selected as a best model, which shows better kinematic performances compared to the basis of the pre-existing model.

• Journal of Life Science
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• v.7 no.4
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• pp.336-341
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• 1997

This study was conducted to investigate the effects of carcass grade on the hardness, myofibrillar fragmentations index, protein extractability and Mg-ATPase activity of myofibril and actomyosin obtained from 1, 2, 3 and D carcass grade)subgrade) in Korean native cow. Proximate component, hardness, chewiness, myofibril fragmentation index, protein extractability and Mg-ATPase activity if myofibril or actomyosin were not significantly different between 1st and 2nd carcass grade loin. The hardness and chewiness of 2nd carcass grade loin's were significantly lower than 3th grade loin's, but the myofibril fragmentation index, sarcoplasmic protein extractability and Mg-ATPase activity of myofibril were higher. The myofibrillar protein extractability and Mg-ATPase activity of actomyosin obtained from 3th carcase grade loin's were significantly higher than D grade loin's, but the hardness, chewiness and stroma protein extractability were lower. In conclusion, the degree of toughness in Korean native cow's loin was not significantly different between 1st and 2nd grade, but 3rd and D carcass grade were significantly higher, regardless of before and after aging.

• Korean Journal of Veterinary Research
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• v.61 no.3
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• pp.24.1-24.5
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• 2021

A 4-year-old Holstein cow with progressive atrophy and ataxia was submitted for diagnosis. The postmortem examination revealed multifocal yellowish nodules in the pulmonary parenchyma and vegetative masses in the mitral and tricuspid valve of the heart. Both kidneys were severely enlarged, with multiple yellow nodules on the parenchyma. Histopathologically, pulmonary abscesses, vegetative endocarditis, suppurative glomerulonephritis, and fibrino-purulent arthritis were observed. The tiny β-hemolytic bacterial colonies were isolated from the lesions and identified as Arcanobacterium haemolyticum by the VITEK 2 system (bioMérieux, USA). This is the first documented report of an A. haemolyticum infection in a Holstein cow in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.259-260
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• 1989

• The Plant Pathology Journal
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• v.17 no.6
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• pp.363.3-363
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• 2001