• The Korean Journal of Physiology and Pharmacology
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• 제2권1호
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• pp.69-76
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• 1998

Cis-diamminedichloroplatinum II (cisplatin), an effective antitumor agent, induces acute renal failure by unknown mechanisms. To investigate direct toxic effects of cisplatin in the renal proximal tubular transport system, OK cell line was selected as a cell model and $Na^+/H^+$ antiport activity was evaluated during a course of cisplatin treatment. The cells grown to confluence were treated with cisplatin for 1 hour, washed, and incubated for up to 48 hours. At appropriate intervals, cells were examined for $Na^+/H^+$ antiport activity by measuring the recovery of intracellular pH (pHi) after acid loading. Cisplatin of less than 50 ${\mu}M$ induced no significant changes in cell viability in 24 hours, but it decreased the viability markedly after 48 hours. In cells exposed to 50 ${\mu}M$ cisplatin for 24 hours, the $Na^+-dependent$ pHi recovery (i.e., $Na^+/H^+$ antiport) was drastically inhibited with no changes in the $Na^+-independent$ recovery. Kinetic analysis of the $Na^+-dependent$ pHi recovery indicated that the Vmax was reduced, but the apparent Km was not altered. The cellular $Na^+$ and $K^+$ contents determined immediately before the transport measurement appeared to be similar in the control and cisplatin group, thus, the driving force for $Na^+-coupled$ transport was not different. These results indicate that cisplatin exposure impairs the $Na^+/H^+$ antiport capacity in OK cells. It is, therefore, possible that in patients treated with a high dose of cisplatin, proximal tubular mechanism for proton secretion (hence $HCO_3^-$ reabsorption) could be attenuated, leading to a metabolic acidosis (proximal renal tubular acidosis).

• Coupled systems mechanics
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• 제1권4호
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• pp.381-395
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• 2012

Dynamic response of Pile Supported Structures is highly depended on Soil Pile Structure Interaction. In this paper, by comparison of experimental and numerical dynamic responses of a prototype jacket offshore platform for both hinge based and pile supported boundary conditions, effect of soil-pile-structure interaction on dynamic characteristics of this platform is studied. Jacket and deck of a prototype platform is installed on a hinge-based case first and then platform is installed on eight skirt piles embedded on continuum monolayer sand. Dynamic characteristics of platform in term of natural frequencies, mode shapes and modal damping are compared for both cases. Effects of adding and removing vertical bracing members in top bay of jacket on dynamic characteristics of platform for both boundary conditions are also studied. Numerical simulation of responses for the studied platform is also performed for both mentioned cases using capability of ABAQUS and SACS software. The 3D model using ABAQUS software is created using solid elements for soil and beam elements for jacket, deck and pile members. Mohr-Coulomb failure criterion and pile-soil interface element are used for considering nonlinear pile soil structure interaction. Simplified modeling of soil-pile-structure interaction effect is also studied using SACS software. It is observed that dynamic characteristics of the system changes significantly due to soil-pile-structure interaction. Meanwhile, both of complex and simplified (ABAQUS and SACS, respectively) models can predict this effect accurately for such platforms subjected to dynamic loading in small range of deformation.

• Coupled systems mechanics
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• 제2권4호
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• pp.329-348
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• 2013

This study aims at observing the coupling behaviours between suspended ceilings and partition walls in terms of their global seismic performance using full-scale shake table tests. The suspended ceilings with planar dimensions of $6.0m{\times}3.6m$ were tested with two types of panels: acoustic lay-in and metal clip-on panels. They were further categorized as seismic-braced, seismic-unbraced, and non-seismic installations. Also, two configurations of 2.7 m high partition wall specimens, with C-shape and I-shape in the plane layouts, were tested. In total, seven ceiling-partition-coupling (CPC) specimens were tested utilizing a unidirectional seismic simulator. The test results indicate that the damage patterns of the tested CPC systems included failure of the ceiling grids, shearing-off of the wall top railing, and, most destructively, numerous partial detachments and falling of the ceiling panels. The loss of panels was mostly concentrated near the center of the tested partition wall. The testing results also confirmed that the failure mode of the non-seismic CPC systems was brittle: The whole system would collapse suddenly all at once when the magnitude of the inputs hit the capacity threshold, rather than displaying progressive damage. Overall, the seismic capacity of the unbraced and braced CPC systems could be up to 1.23 g and 2.67 g, respectively; these accelerations were both achieved at the base of the partition wall. Nonetheless, for practical applications, it is noteworthy that the three-dimensional nature of seismic excitations and the size effect of the ceiling area are parameters that exacerbate the CPC's seismic response so that their actual capacity may be dramatically decreased, leading to important losses even in moderate seismic events.

• Steel and Composite Structures
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• 제26권4호
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• pp.473-484
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• 2018

Free vibration of cross-ply laminated plates using a higher-order shear deformation theory is studied. The arbitrary number of layers is oriented in symmetric and anti-symmetric manners. The plate kinematics are based on higher-order shear deformation theory (HSDT) and the vibrational behaviour of multi-layered plates are analysed under simply supported boundary conditions. The differential equations are obtained in terms of displacement and rotational functions by substituting the stress-strain relations and strain-displacement relations in the governing equations and separable method is adopted for these functions to get a set of ordinary differential equations in term of single variable, which are coupled. These displacement and rotational functions are approximated using cubic and quantic splines which results in to the system of algebraic equations with unknown spline coefficients. Incurring the boundary conditions with the algebraic equations, a generalized eigen value problem is obtained. This eigen value problem is solved numerically to find the eigen frequency parameter and associated eigenvectors which are the spline coefficients.The material properties of Kevlar-49/epoxy, Graphite/Epoxy and E-glass epoxy are used to show the parametric effects of the plates aspect ratio, side-to-thickness ratio, stacking sequence, number of lamina and ply orientations on the frequency parameter of the plate. The current results are verified with those results obtained in the previous work and the new results are presented in tables and graphs.

• 한국자동차공학회논문집
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• 제18권6호
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• pp.130-137
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• 2010

Due to test facility and specialty, it is physically difficult to conduct the modal tests of large-sized structures such as truck, bus and airplane. So, in case of a large-sized truck, the mode analysis on a full vehicle model comprised of reliable cabin, frame, and deck has been generally performed. However, the reliability of overall vibrational characteristics of the analytic model has not been fairly guaranteed by the testified models of each subsystem owing to the existence of cab suspension and the nonlinear mounting between a chassis frame and a special deck system. In this paper, a method to find out the modal characteristics of a large-sized military truck is presented. New modal test equipment is developed to set the boundary conditions of three military truck variants as close as a free-free condition. And the mode analysis method using coupled structure and dynamic models is established to consider the above-mentioned dynamic non-linearities of the vehicle itself. The usefulness of the suggested method is verified by comparing with the modal test results. Finally, the modal parameters of the final variant are extracted using the proved analytic method.

• 한국동물생명공학회지
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• 제35권2호
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• pp.155-162
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• 2020

Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC₅₀ values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

• Structural Engineering and Mechanics
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• 제58권2호
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• pp.199-216
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• 2016

A large number of bridges were built several decades ago, and most of which have gradually suffered serious deteriorations or damage due to the increasing traffic loads, environmental effects, and inadequate maintenance. However, very few studies were conducted to investigate the vibration behaviors of a damaged bridge under moving vehicles. In this paper, the vibration behaviors of such vehicle-bridge system are investigated in details, in which the effects of the concrete cracks and bridge surface roughness are particularly considered. Specifically, two vehicle models are introduced, i.e., a simplified four degree-of-freedoms (DOFs) vehicle model and a more complex seven DOFs vehicle model, respectively. The bridges are modeled in two types, including a single-span uniform beam and a full scale reinforced concrete high-pier bridge, respectively. The crack zone in the reinforced concrete bridge is considered by a damage function. The bridge and vehicle coupled equations are established by combining the equations of motion of both the bridge and vehicles using the displacement relationship and interaction force relationship at the contact points between the tires and bridge. The numerical simulations and verifications show that the proposed modeling method can rationally simulate the vibration behaviors of the damaged bridge under moving vehicles; the effect of cracks on the impact factors is very small and can be neglected for the bridge with none roughness, however, the effect of cracks on the impact factors is very significant and cannot be neglected for the bridge with roughness.

• Earthquakes and Structures
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• 제6권1호
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• pp.71-87
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• 2014

Interaction between closely-spaced buildings subject to earthquake induced strong ground motions, termed in the literature as "seismic pounding", occurs commonly during major seismic events in contemporary congested urban environments. Seismic pounding is not taken into account by current codes of practice and is rarely considered in practice at the design stage of new buildings constructed "in contact" with existing ones. Thus far, limited research work has been devoted to quantify the influence of slab-to-slab pounding on the inelastic seismic demands at critical locations of structural members in adjacent structures that are not aligned in series. In this respect, this paper considers a typical case study of a "new" reinforced concrete (R/C) EC8-compliant, torsionally sensitive, 7-story corner building constructed within a block, in bi-lateral contact with two existing R/C 5-story structures with same height floors. A non-linear local plasticity numerical model is developed and a series of non-linear time-history analyses is undertaken considering the corner building "in isolation" from the existing ones (no-pounding case), and in combination with the existing ones (pounding case). Numerical results are reported in terms of averages of ratios of peak inelastic rotation demands at all structural elements (beams, columns, shear walls) at each storey. It is shown that seismic pounding reduces on average the inelastic demands of the structural members at the lower floors of the 7-story building. However, the discrepancy in structural response of the entire block due to torsion-induced, bi-directionally seismic pounding is substantial as a result of the complex nonlinear dynamics of the coupled building block system.

• 한국터널지하공간학회 논문집
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• 제6권4호
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• pp.279-290
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• 2004

이 논문은 I-단면 곡선강지보 (curved steel rib)의 등분포 하중 하에서, 비틀림 (warping)을 포함한, 외평면 (out-of-plane)의 안정성을 해석하였다. 미분구적법 (differential quadrature method, DQM)을 이용하여 다양한 경계조건, 굽힘각 (opening angles)과 강성매개변수 (stiffness parameter)에 따른 임계하중 (critical loads) 및 임계하중 매개변수 (dimensionless buckling parameter)를 계산하였고, Differential quadrature method (DQM)의 해석결과를 타 이론과 비교 분석 하였다. 또한 두 경계조건 (고정-고정, 고정-단순지지)하에서의 새로운 결과를 제시하였고, DQM을 이용한 곡선강지보의 좌굴해석은, 비교적 적은 요소 (grid points)를 사용하고도, 타 이론에 의한 해석적 결과에 비해 정확성과 안정성을 보여주었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7473-7482
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• 2013

The G-protein coupled receptor 87 (GPR87) is a recently discovered orphan GPCR which means that the search of their endogenous ligands has been a novel challenge. GPR87 has been shown to be overexpressed in squamous cell carcinomas (SCCs) or adenocarcinomas in lungs and bladder. The 3D structure of GPR87 was here modeled using two templates (2VT4 and 2ZIY) by a threading method. Functional assignment of GPR87 by SVM revealed that along with transporter activity, various novel functions were predicted. The 3D structure was further validated by comparison with structural features of the templates through Verify-3D, ProSA and ERRAT for determining correct stereochemical parameters. The resulting model was evaluated by Ramachandran plot and good 3D structure compatibility was evidenced by DOPE score. Molecular dynamics simulation and solvation of protein were studied through explicit spherical boundaries with a harmonic restraint membrane water system. A DRY-motif (Asp-Arg-Tyr sequence) was found at the end of transmembrane helix3, where GPCR binds and thus activation of signals is transduced. In a search for better inhibitors of GPR87, in silico modification of some substrate ligands was carried out to form polar interactions with Arg115 and Lys296. Thus, this study provides early insights into the structure of a major drug target for SCCs.