• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.6
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• pp.130-137
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• 2010

Due to test facility and specialty, it is physically difficult to conduct the modal tests of large-sized structures such as truck, bus and airplane. So, in case of a large-sized truck, the mode analysis on a full vehicle model comprised of reliable cabin, frame, and deck has been generally performed. However, the reliability of overall vibrational characteristics of the analytic model has not been fairly guaranteed by the testified models of each subsystem owing to the existence of cab suspension and the nonlinear mounting between a chassis frame and a special deck system. In this paper, a method to find out the modal characteristics of a large-sized military truck is presented. New modal test equipment is developed to set the boundary conditions of three military truck variants as close as a free-free condition. And the mode analysis method using coupled structure and dynamic models is established to consider the above-mentioned dynamic non-linearities of the vehicle itself. The usefulness of the suggested method is verified by comparing with the modal test results. Finally, the modal parameters of the final variant are extracted using the proved analytic method.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.2
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• pp.155-162
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• 2020

Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC₅₀ values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

• Structural Engineering and Mechanics
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• v.58 no.2
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• pp.199-216
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• 2016

A large number of bridges were built several decades ago, and most of which have gradually suffered serious deteriorations or damage due to the increasing traffic loads, environmental effects, and inadequate maintenance. However, very few studies were conducted to investigate the vibration behaviors of a damaged bridge under moving vehicles. In this paper, the vibration behaviors of such vehicle-bridge system are investigated in details, in which the effects of the concrete cracks and bridge surface roughness are particularly considered. Specifically, two vehicle models are introduced, i.e., a simplified four degree-of-freedoms (DOFs) vehicle model and a more complex seven DOFs vehicle model, respectively. The bridges are modeled in two types, including a single-span uniform beam and a full scale reinforced concrete high-pier bridge, respectively. The crack zone in the reinforced concrete bridge is considered by a damage function. The bridge and vehicle coupled equations are established by combining the equations of motion of both the bridge and vehicles using the displacement relationship and interaction force relationship at the contact points between the tires and bridge. The numerical simulations and verifications show that the proposed modeling method can rationally simulate the vibration behaviors of the damaged bridge under moving vehicles; the effect of cracks on the impact factors is very small and can be neglected for the bridge with none roughness, however, the effect of cracks on the impact factors is very significant and cannot be neglected for the bridge with roughness.

• Earthquakes and Structures
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• v.6 no.1
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• pp.71-87
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• 2014

Interaction between closely-spaced buildings subject to earthquake induced strong ground motions, termed in the literature as "seismic pounding", occurs commonly during major seismic events in contemporary congested urban environments. Seismic pounding is not taken into account by current codes of practice and is rarely considered in practice at the design stage of new buildings constructed "in contact" with existing ones. Thus far, limited research work has been devoted to quantify the influence of slab-to-slab pounding on the inelastic seismic demands at critical locations of structural members in adjacent structures that are not aligned in series. In this respect, this paper considers a typical case study of a "new" reinforced concrete (R/C) EC8-compliant, torsionally sensitive, 7-story corner building constructed within a block, in bi-lateral contact with two existing R/C 5-story structures with same height floors. A non-linear local plasticity numerical model is developed and a series of non-linear time-history analyses is undertaken considering the corner building "in isolation" from the existing ones (no-pounding case), and in combination with the existing ones (pounding case). Numerical results are reported in terms of averages of ratios of peak inelastic rotation demands at all structural elements (beams, columns, shear walls) at each storey. It is shown that seismic pounding reduces on average the inelastic demands of the structural members at the lower floors of the 7-story building. However, the discrepancy in structural response of the entire block due to torsion-induced, bi-directionally seismic pounding is substantial as a result of the complex nonlinear dynamics of the coupled building block system.

• Journal of Korean Tunnelling and Underground Space Association
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• v.6 no.4
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• pp.279-290
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• 2004

The differential quadrature method (DQM) for a system of coupled differential equations governing the elastic stability of thin-walled curved members is presented, and is applied to computation of the eigenvalues of out-of-plane buckling of curved beams subjected to uniformly distributed radial loads including a warping contribution. Critical loads with warping, which were found to be significant, are calculated for a single-span wide-flange beam with various end conditions, opening angles, and stiffness parameters. The results are compared with the exact methods available. New results are given for the case of both ends clamped and clamped-simply supported ends without comparison since no data are available The differential quadrature method gives good accuracy and stability compared with previous theoretical results.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7473-7482
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• 2013

The G-protein coupled receptor 87 (GPR87) is a recently discovered orphan GPCR which means that the search of their endogenous ligands has been a novel challenge. GPR87 has been shown to be overexpressed in squamous cell carcinomas (SCCs) or adenocarcinomas in lungs and bladder. The 3D structure of GPR87 was here modeled using two templates (2VT4 and 2ZIY) by a threading method. Functional assignment of GPR87 by SVM revealed that along with transporter activity, various novel functions were predicted. The 3D structure was further validated by comparison with structural features of the templates through Verify-3D, ProSA and ERRAT for determining correct stereochemical parameters. The resulting model was evaluated by Ramachandran plot and good 3D structure compatibility was evidenced by DOPE score. Molecular dynamics simulation and solvation of protein were studied through explicit spherical boundaries with a harmonic restraint membrane water system. A DRY-motif (Asp-Arg-Tyr sequence) was found at the end of transmembrane helix3, where GPCR binds and thus activation of signals is transduced. In a search for better inhibitors of GPR87, in silico modification of some substrate ligands was carried out to form polar interactions with Arg115 and Lys296. Thus, this study provides early insights into the structure of a major drug target for SCCs.

• Environmental Analysis Health and Toxicology
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• v.7 no.1_2
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• pp.53-57
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• 1992

Amount of lead burden in a tissue reflects poisoning of lead in that tissue, so is the removal of lead directly connected to curement of lead poisoning. The purpose of present study was to investigate the relative effects of penicillamine and thiamine tetrahydrofurfuryl disulfide (TTFD) or thiamine propyl disulfide (TPD) in the removal of lead from rat brain tissue treated with excessive lead. Wistar rat pups of both sexes were used in this experiment. Within 1 day of parturition, experimental mothers nursing their pups as well as rat pups were given drinking water containing 0.2% lead acetate, TTFD 20mg/1.2 L (2 mg/kg/day), TPD 20 mg/1.2 L (2mg/kg/day), penicillamine 40 mg/1.2 L (40 mg/kg/day), 0.2% lead acetate+TTFD 20mg/1.2 L (2 mg/kg/day), 0.2% lead acetate+ TPD 20 mg/1.2 L (2 mg/kg/day) or 0.2% lead acetate+ penicillamine 40 mg/1.2 L (40 mg/kg/day) ad libitum, throughout the entire period of experiment. Rat pups in the control group received normal tap water. The animals were sacrificed by decapitation on the day when they become 2 or 8 weeks of age. Brains were dissected into five regions: telencephalon, diencephalon, midbrain, pons/medulla and cerebellum. The dissected brain tissues were lyophillized and then solubilized by acid mixture (nitric acid + sulfuric acid). Lead levels in the solubilized brain tissues were measured by the inductively coupled plasma. In lead-exposed rats, lead levels were significantly higher than those of control group in all brain legions, lead levels in brain regions of TTFD or TPD group were generally lower than those of control group. The simultaneous administration of lead with TTFD or TPD to animals caused significant decrement of lead from all brain regions. In the elimination of lead from brain regions, effectiveness of TTFD or TPD was equivalant to penicillamine.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.371-374
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• 2012

본 연구는 "사이클론과 관성충돌 및 백필터의 제진원리를 일체화한 고효율 멀티 제진시스템의 최적화 설계를 위한 수치 해석적 연구(I) : 집진기 입구 최적화 설계"에 이은 일련의 연구로서 앞선 연구에서 도출된 사이클론부 하단 벤츄리(Venturi)에서의 강한 하향 기류의 가속에 따른 분진 재비산 가능성에 대한 추가적 검토를 위한 연구이다. 사이클론부를 통과한 기류가 상향 흐름으로 방향을 전환할때 좀 더 가속시켜 빠르게 곡률반경을 형성하여 조대 입자의 분리를 극대화시키기 위한 목적으로 사이클론부 하단을 벤츄리 형상으로 설계하였으나, 유동장 분석 결과 벤츄리를 통과한 처리가스 흐름이 가속되면서 호퍼 하단까지 약 4~5 m/s의 강한 하향 흐름을 형성하고 호퍼 하부의 말단 부근에서 상향 흐름으로 방향 전환을 하고 있는 것으로 예측됨에 따라 설계 의도와는 달리 벤츄리 설치시 호퍼 하단에 포집된 분진의 재비산을 예방하는데는 크게 역할을 하지 못하는 것으로 나타났다.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.367-370
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• 2012

본 연구는 하나의 장치 내에서 "싸이클론-관성충돌-여과포 집진"이라는 다단 제진 단계가 동시에 이루어져 입경 분포별로 순차적인 제진이 가능한 멀티 집진기의 처리가스 제어 효율을 극대화시킬 수 있는 기류 흐름을 얻기 위한 최적화 설계 조건을 검토하는 것이다. 우선 1단계 제진 과정인 원심력을 이용하여 조대입자의 유선 이탈을 촉진시킬 수 있는 사이클론 유동을 최적화시키기 위한 집진기 입구 형상 설계 검토를 위해 수치 해석적 연구를 수행하였다. 그 결과, 멀티집진기 입구 형상을 일반적인 설계 방식인 접선 유입식으로만 설계한 것에 비해 선회류를 한번 더 가속화시킬 수 있는 가이드 베인을 추가로 설계한 경우 선회류의 pitch가 짧고 강하게 형성되어 사이클론부에서 조대입자의 유선 이탈을 촉진시키는데 매우 효과적인 것으로 예측되었다. 단, 사이클론부 하단 벤츄리 형상으로 인해 약 4~5 m/s의 강한 하향 흐름이 호퍼 하단까지 형성되고 있어 탈리 분진의 재비산 문제가 발생할 가능성이 크므로 벤츄리 형상 설치 유무에 대한 영향도 추가적으로 연구가 필요할 것으로 판단된다.

• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.159-165
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• 2014

In this study, Krill Eupausia superba oil was extracted using different solvents and supercritical carbon dioxide (SC-$CO_2$). During SC-$CO_2$ extraction, the pressure was set at 40 MPa and temperatures ranged from $40^{\circ}C$ to $55^{\circ}C$. We examined the differences in volatile compounds and safety profiles among extraction methods. Volatile compounds were determined using the thermal desorption system integrated with gas chromatography-mass spectrometry (GC-MS). Heavy metal content was analyzed by inductively coupled plasma mass spectrometry (ICP-MS). According to our results 10 volatile compounds were identified in krill sample. After SC-$CO_2$ extraction of oil, the concentrations of volatile compounds decreased, but increased after solvent extraction. In krill, heavy metal concentrations remained within the permissible limit. Moreover, Zn and Fe which have health benefits were detected at high concentrations. During a 90 days storage period at different temperatures, microbial activity was found to be lowest in SC-$CO_2$ extracted residues. Thus, the quality of krill oil and the residues obtained using SC-$CO_2$ extraction was higher and the oil was safer than those obtained using conventional solvent extraction. These results can be applied to the food industry to maintain high quality krill products.