• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.125-129
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• 2009

The genetic diversity plays a significant role in determining a species' survival and perseverance. Endangered species often lack genetic variation, which makes them vulnerable to numerous dangers of extinction including selection, genetic drifts and human interference. Knowing an endangered species' genetic background greatly enhances conservation efforts since it reveals why, what and how to conserve that species. Cotoneaster wilsonii is an endangered plant species endemic to Ulleung island, but not enough genetic research has been done on this taxon for its effective conservation plans. In this study, three populations of C. wilsonii in Ulleung island underwent allozyme analysis through starch gel electrophoresis. 10 loci were analyzed and F-statistics was calculated. Overall data indicated that C. wilsonii possessed low genetic diversity with intense inbreeding, heterozygote deficiency and low differentiation among populations. These results implied that C. wilsonii was recently introduced to the Ulleung island from ancestor species, and did not have much time to differentiate. Current status of C. wilsonii habitats is very fragile and vulnerable, with increasing tourism constantly threatening the species' survival. It is very likely that C. wilsonii will become extinct in near future unless organized conservation protects its populations and genetic diversity.

• Korean Journal of Medicinal Crop Science
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• v.27 no.3
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• pp.194-201
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• 2019

Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.

• Korean Journal of Medicinal Crop Science
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• v.27 no.6
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• pp.412-418
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• 2019

Background: Previously, studies have observed that the leaf extract of Cotoneaster wilsonii Nakai has potent antioxidant and anti-inflammatory activites. Therefore, further research was conducted to separate the active antioxidant and anti-inflammatory compounds in the leaf of the C. wilsonii. Methods and Results: The anti-oxidant effects were evaluated by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, estimating the totla phenolic content (TPC) and total flavonoid content (TFC), and using reducing power assay method. The ethyl acetate (EtOAc) fraction demonstrated the highest DPPH radical scavenging activity (IC₅₀; 9.69 ㎍/㎖), and the highest TPC and TFC (345.98 mg·GAE/g, and 74.23 mg·QE/g). Moreover, it indicated the highest effects on nitric oxide production. Therefore, the active compound was separated using the EtOAc fraction, determined on the basis of spectral data, and identified as (+)-catechin. Conclusions: The first compound separated was made from the leaf of C. wilsonii was (+)-catechin, which produced potent anti-oxidant and anti-inflammatory effects.

• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.133-143
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• 2003

The three wild populations including unrecorded two wild populations and Do-dong population, where designated as a Natural Monument NO 51, of Cotoneaster wilsonii in Ulnung Island were studied. The population at Yilmoldae, western coast of the island is in the threat by the visitors, while the population at Tonggumi is well conserved due to the geomorphologically difficult to access . The existing 25 to 50 maturing individuals are are potentially vital sources fer the island species recovery projects in the future. There are no statistical difference in the floral characteristics of the species such as both length and width of the petals, while statistical difference were shown from the leaf characteristics such as length, width and area of the leaves in the three studies areas.