• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.92-97
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• 2012

Antimicrobial peptides (AMPs) are important components of living organisms acting against Gram-negative and Gram-positive bacterial and fungal pathogens. Cathelicidin human peptides have a variety of biological activities that can be used in clinical applications. AMPs are not produced naturally in large quantities, and chemical synthesis is also economically impractical, especially for long peptides. Therefore, as an alternative, heterologous expression of AMPs by recombinant techniques has been studied as a means to reduce production costs. E. coli is an excellent host for the expression of AMPs, as well as other recombinant proteins, because of the low cost involved and its easy manipulation. However, overexpression of AMPs in E. coli has been shown to cause difficulties resulting from the toxicity of the subsequently produced AMPs. Therefore, fusion expression was theorized to be a solution to this problem. In this study, AMPs were expressed as fused proteins with the glutathione S-transferase (GST) binding protein to protect against the toxicity of AMPs when expressed in E. coli. The LL37, and hybrid gaegurin and LL37 (GGN4(1-16)-LL37(17-32), which we designated as GL32, peptides were expressed as GST-fusion proteins in E. coli and the fusion proteins were then purified by affinity columns. The purified peptides were obtained by removal of GST and were confirmed by western blot analysis. The purified antimicrobial peptides then demonstrated antimicrobial activities against Gram-negative and Gram-positive bacterial strains.

• The KIPS Transactions:PartD
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• v.15D no.6
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• pp.793-802
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• 2008

The development methods which utilize OSSs have been tried as new alternative to solve limits of the previous software developments such as the quality of software, and time and cost of developments. Especially, small and medium companies are suffering from difficulty in applying large-scale development methodology whenever they develop softwares. Therefore, in the current situation that demand for small-scale development methodology is increasing, the methods of utilizing OSSs can become an efficient way to save costs and reduce a development period. Accordingly, analysis of open-source is no fixed procedure or method to utilize open-source for software developments in the field. In this thesis, to solve such problems, we propose the procedures and methods for identifying and selecting suitable open-source, and effective methods for improvement and integration through least modification on the basis of synthesis of existing researches and experiences in development projects. For selection of OSSs, we did identify correct requirements for the software to be developed, investigate the open-source just matching with such requirements, draw a candidate index, establish assessment criteria, and the consequently present a method to select OSSs. And, we deduced the validity and improvement of each detailed activity from practical application to an actual project and assessment.

• Management & Information Systems Review
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• v.36 no.3
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• pp.239-254
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• 2017

This study examines the relationship between R&D spending and patent rights, which are suggested by firms as a way to increase their firm value. Specifically, we examine the relationship between research and development costs and patent rights, and see if there are any differences in the influence of two variables on firm value. The period of this study is from 2005 to 2016, and the sample of companies used in the research including the patenting companies is composed of 333 companies in total. The results of the study are as follows. First, the cost of R&D expenditure and capitalized R&D expenditure showed a significant positive correlation with patents. Second, R&D spending did not show a significant relationship with firm value, but patent rights showed a significant positive correlation with firm value. However, firms that spend a large amount of R&D expenditure (RDD), such as research and development expenses, showed a significant positive (+) value and a patent dummy (PATD). Third, in the analysis of the difference between the dummy of research and development and the patent dummy, the enterprise value of a company that invested a lot of patents and research and development expenses was high. The contribution of this study is to examine the relevance of corporate value to R&D investment for patents. On the other hand, there are various variables that can be used as a sample of patents.

• Journal of Korean Tunnelling and Underground Space Association
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• v.21 no.1
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• pp.137-153
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• 2019

Estimating tunnel construction time and costs are the most fundamental part of a tunnel project planning, which has been generally assessed on a deterministic basis until now. In this paper, excavation cycle time was investigated for two road tunnels and one subway tunnel, and the results were compared with the Standard of Construction Estimate (SE), which is made for the estimation of construction time and cost in a design stage. The results show that the difference in cycle time between SE and actual cycle time is 50%, 7% and 31% respectively for the three tunnels, which means that SE does not reflect practical operation time. The major reasons of the difference are skilled level of tunneling workers, the change of operation sequences for more effective operations, much more complicated working atmosphere in a tunnel than the assumption of SE etc. Finally, even though the results can not be generalized since investigated tunnels are only 3, but it is thought that SE needs to be upgraded into the model able to consider quite common situations through additional tunnel investigation and studies in the future.

• The Journal of Engineering Geology
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• v.34 no.1
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• pp.39-49
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• 2024

The construction of underground infrastructure is garnering growing increasing research attention owing to population concentration and infrastructure overcrowding in urban areas. An important associated task is establishing a monitoring system to evaluate stability during infrastructure construction and operation, which relies on developing techniques for ground investigation that can evaluate ground stability, verify design validity, predict risk, facilitate safe operation management, and reduce construction costs. The method proposed here uses satellite imaging in a cost-effective and accurate ground investigation technique that can be applied over a wide area during the construction and operation of infrastructure. In this study, analysis was performed using Synthetic Aperture Radar (SAR) data with the time-series radar interferometric technique to observe surface displacement during the construction of urban underground roads. As a result, it was confirmed that continuous surface displacement was occurring at some locations. In the future, comparing and analyzing on-site measurement data with the points of interest would aid in confirming whether displacement occurs due to tunnel excavation and assist in estimating the extent of excavation impact zones.

• Journal of Korea Water Resources Association
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• v.44 no.10
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• pp.787-795
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• 2011

Extreme water events such as heavy rainfalls due to recent climate change are continually increasing and their scale has also shown an increasing trend. To overcome these natural disasters, this policy study suggests securing lateral river space as an effective method for extreme flood. To support the importance of restoration and expansion of lateral river space, Gumi upstream region of the Nakdong River basin was chosen as a target area and flood reduction analysis of the washland by using LISFLOOD model have been examined. The 500-year frequency flood was simulated for the estimation of possibly occurable flood level and it turns out that the secured lateral river space on the selected site effectively lowers about 0.53 m flood level and reduces the flood damage of the city on the lower reaches of the river. In addition, based on this result, multilateral river space securing plans were compared, and conservation easement and natural disaster insurance were suggested for sustainable and cost-effective alternatives. The costs of land purchase and conservation easement for securing the river space were also compared.

• Environmental and Resource Economics Review
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• v.29 no.3
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• pp.363-388
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• 2020

The purposes of this study are to not only estimate optimal harvests and efforts using the surplus production methods for Spanish mackerel caught by multiple fishing gears, but provide dynamic optimal fisheries management for these gears using the current value Hamiltonian method. To achieve the above purposes this study uses several models such as Gavaris's general linear model for standardizing fishing efforts, surplus production method for estimating biological and technological coefficients, current value Hamiltonian method for estimating dynamic optimal harvest and efforts, and sensitivity analysis for diagnosing economic influences of these fisheries. As a result, this study showed that Spanish mackerel was overfished by multiple fishing gears based on surplus production method and the current value Hamiltonian method. Also, this study found that when the price and cost proportionally changed, the optimal harvest and fishing effort sensitively responded to the stock level of Spanish mackerel. Next, this study suggested that the multiple fishing gears for Spanish mackerel should reduce unnecessary costs such as operating time or inefficient fuel consumption. Finally, this study provided reasons Spanish mackerel should be included in the TAC system in a view of profit maximization based on sustainable use of the Spanish mackerel.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.05a
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• pp.121-122
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• 2023

As containerized maritime transport began in earnest, the size of container ships has steadily increased, and recently, the operation of 24,000 TEU-class vessels has become regular. However, concerns about the efficiency and productivity of such mega container ships from a port operational perspective have continued to be raised. The 10^th Busan International Port Conference requested an in-depth study on the trends of container ship enlargement by analyzing the order status of ultra-large container ships from major global liners. Generally, the factor that drives the upsizing of ships is the realization of economies of scale that lowers transportation costs per TEU, which leads to a higher level of cost reduction per unit transportation compared to the increase in fuel consumption due to transporting large amounts of cargo with a single ship. However, it is necessary to examine whether this trend of container vessel enlargement is feasible for port operations. To this end, this study compares and analyzes the productivity and efficeiency of different ship sizes to evaluate the effect of ship size on port operations.

• Korean Journal of Construction Engineering and Management
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• v.24 no.6
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• pp.66-73
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• 2023

The construction industry suffers losses due to failures in demand forecasting due to price fluctuations in construction raw materials, increased user costs due to project cost changes, and lack of forecasting system. Accordingly, it is necessary to improve the accuracy of construction raw material price forecasting. This study aims to predict the price of construction raw materials and verify applicability through the improvement of the Data Refactor technique. In order to improve the accuracy of price prediction of construction raw materials, the existing data refactor classification of low and high frequency and ARIMAX utilization method was improved to frequency-oriented and ARIMA method utilization, so that short-term (3 months in the future) six items such as construction raw materials lumber and cement were improved. ), mid-term (6 months in the future), and long-term (12 months in the future) price forecasts. As a result of the analysis, the predicted value based on the improved Data Refactor technique reduced the error and expanded the variability. Therefore, it is expected that the budget can be managed effectively by predicting the price of construction raw materials more accurately through the Data Refactor technique proposed in this study.

• Information Systems Review
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• v.25 no.4
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• pp.27-45
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• 2023

The importance of information security has grown alongside the development of information and communication technology. However, companies struggle to select suitable countermeasures within their limited budgets. Sönmez and Kılıç (2021) proposed a model using AHP and mixed integer programming to determine the optimal investment combination for mitigating information security breaches. However, their model had limitations: 1) a lack of objective measurement for countermeasure efficacy against security threats, 2) unrealistic scenarios where risk reduction surpassed pre-investment levels, and 3) cost duplication when using a single countermeasure for multiple threats. This paper enhances the model by objectively quantifying countermeasure efficacy using the beta probability distribution. It also resolves unrealistic scenarios and the issue of duplicating investments for a single countermeasure. An empirical analysis was conducted on domestic SMEs to determine investment budgets and risk levels. The improved model outperformed Sönmez and Kılıç's (2021) optimization model. By employing the proposed effectiveness measurement approach, difficulty to evaluate countermeasures can be quantified. Utilizing the improved optimization model allows for deriving an optimal investment portfolio for each countermeasure within a fixed budget, considering information security costs, quantities, and effectiveness. This aids in securing the information security budget and effectively addressing information security threats.